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IMAGING REDOX STATE HETEROGENEITY WITHIN INDIVIDUAL EMBRYONIC STEM CELL COLONIES

Journal of Innovative Optical Health Sciences 2011年第3期4卷 279-288页

作者： HE N.XU RUSSELL C.ADDIS DAVIDA F.GOINGS SHOKO NIOKA BRITTON CHANCE john D.gearhart LIN Z.LI Molecular Imaging Laboratory Department of Radiology University of PennsylvaniaSchool of Medicine PhiladelphiaPA 19104USA Institute for Regenerative Medicine and Department of Cell and Developmental Biology University of Pennsylvania School of Medicine PhiladelphiaPA 19104USA Britton Chance Laboratory of Redox Imaging johnson Research Foundation Department of Biochemistry and Biophysics University of Pennsylvania School of Medicine PhiladelphiaPA 19104USA Institute of Translational Medicine and Therapeutics University of Pennsylvania PhiladelphiaPA 19104USA

Redox state mediates embryonic stem cell(ESC)differentiation and thus offers an important complementary approach to understanding the pluripotency of stem *** redox ratio(NADH/(Fp t NADH)),where NADH is the reduced form of nicotinamide adenine dinucleotide and Fp is the oxidizedflavoproteins,has been established as a sensitive indicator of mitochondrial redox *** this paper,we report our redox imaging data on the mitochondrial redox state of mouse ESC(mESC)colonies and the implications *** low-temperature NADH/Fp redox scanner was employed to image mESC colonies grown on a feeder layer of gamma-irradiated mouse embryonicfibroblasts(MEFs)on glass cover *** result showed significant heterogeneity in the mitochondrial redox state within individual mESC colonies(size:~200-440μm),exhibiting a core with a more reduced state than the *** more reduced state positively correlates with the expression pattern of Oct4,a well-established marker of *** observation is thefirst to show the heterogeneity in the mitochondrial redox state within a mESC colony,suggesting that mitochondrial redox state should be further investigated as a potential new biomarker for the stemness of embryonic stem cells.

关键词： Redox imaging pluripotency NADH flavoproteins redox ratio

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Handbook of Stem Cells

引用

丛书名： v. 1

2004年

作者： Robert Lanza john gearhart Brigid Hogan Douglas Melton Roger Pedersen James Thomson Michael West

ISBN: (数字)9780080533735

ISBN: (纸本)9780124366435

New discoveries in the field of stem cell research have frequently appeared in the news and in scientific literature. Research in this area promises to lead to new therapies for cancer, heart disease, diabetes, and a wide variety of other diseases. This two-volume reference integrates this exciting area of biology, combining the prerequisites for a general understanding of adult and embryonic stem cells, the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations, as well as a presentation by the world's experts of what is currently known about each specific organ system. The editors of the Handbook of Stem Cells include: Robert Lanza, Helen Blau, john gearhart, Brigid Hogan, Douglas Melton, Malcolm Moore, Roger Pedersen, E. Donnall Thomas, James Thomson, Catherine Verfaillie, Irving Weissman, and Michael West. The Editorial Board includes: W. French Anderson, Peter Andrews, Anthony Atala, Jose Cibelli, Giulio Cossu, Robert Edwards, Martin Evans, Elaine Fuchs, Margaret Fuller, Fred Gage, Richard Gardner, Margaret Goodell, Ronald Green, William Haseltine, Joseph Itskovitz-Eldor, Rudolf Jaenisch, Ihor Lemischka, Dame Anne McLaren, Richard Mulligan, Stuart Orkin, Martin Pera, Benjamin Reubinoff, Janet Rossant, Hans Scholer, Austin Smith, Evan Snyder, Davor Solter, Alan Trounson, and Leonard Zon. This comprehensive set should be a much-needed addition to the library of students and researchers alike.

关键词： Stem cells.

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