IMAGING REDOX STATE HETEROGENEITY WITHIN INDIVIDUAL EMBRYONIC STEM CELL COLONIES

作     者：HE N.XU RUSSELL C.ADDIS DAVIDA F.GOINGS SHOKO NIOKA BRITTON CHANCE JOHN D.GEARHART LIN Z.LI 

作者机构：Molecular Imaging LaboratoryDepartment of Radiology University of PennsylvaniaSchool of Medicine PhiladelphiaPA 19104USA Institute for Regenerative Medicine and Department of Cell and Developmental Biology University of PennsylvaniaSchool of Medicine PhiladelphiaPA 19104USA Britton Chance Laboratory of Redox Imaging Johnson Research Foundation Department of Biochemistry and Biophysics University of PennsylvaniaSchool of Medicine PhiladelphiaPA 19104USA Institute of Translational Medicine and Therapeutics University of PennsylvaniaPhiladelphiaPA 19104USA 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2011年第4卷第3期

页      面：279-288页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：provided by the Institute for Regenerative Medicine at University of Pennsylvania with an IRM pilot grant(L.Z.Li) 

主　　题：Redox imaging pluripotency NADH flavoproteins redox ratio 

摘      要：Redox state mediates embryonic stem cell(ESC)differentiation and thus offers an important complementary approach to understanding the pluripotency of stem *** redox ratio(NADH/(Fp t NADH)),where NADH is the reduced form of nicotinamide adenine dinucleotide and Fp is the oxidizedflavoproteins,has been established as a sensitive indicator of mitochondrial redox *** this paper,we report our redox imaging data on the mitochondrial redox state of mouse ESC(mESC)colonies and the implications *** low-temperature NADH/Fp redox scanner was employed to image mESC colonies grown on a feeder layer of gamma-irradiated mouse embryonicfibroblasts(MEFs)on glass cover *** result showed significant heterogeneity in the mitochondrial redox state within individual mESC colonies(size:~200-440μm),exhibiting a core with a more reduced state than the *** more reduced state positively correlates with the expression pattern of Oct4,a well-established marker of *** observation is thefirst to show the heterogeneity in the mitochondrial redox state within a mESC colony,suggesting that mitochondrial redox state should be further investigated as a potential new biomarker for the stemness of embryonic stem cells.