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cABYR binds to AKAP3 and Ropporin in the human sperm fibrous sheath

Asian Journal of Andrology 2011年第2期13卷 266-274页

作者： Yan-Feng Li Wei He Arabinda Mandal Young-Hwan Kim Laura Digilio Ken Klotz charles J Flickinger john c herr Department of Urology Daping Hospital Institute of Surgery Research Third Military Medical University Chongqing 400042 China Department of Obstetrics and Gynecology Southwest Hospital Third Military Medical University Chongqing 400038 China center for Research in contraceptive and Reproductive Health Department of Cell BiologyUniversity of Virginia Charlottesville VA 22908 USA

calcium-binding tyrosine phosphorylation-regulated protein （cABYR） is a highly polymorphic calcium-binding tyrosine- and serine-/threonine-phosphorylated fibrous sheath （FS） protein involved in capacitation. A putative domain （amino acids 12-48） homologous to the regulatory subunit of type II cAMP-dependent protein kinase A （RII） dimerisation and A kinase-anchoring protein （AKAP）-binding domains of protein kinase A at the N-terminus suggests that cABYR may self-assemble and bind to AKAPs. Moreover, there is evidence that cABYR has limited interaction with AKAPs. However, further evidence and new relationships between cABYR and other FS proteins, including AKAPs, will be helpful in understanding the basic physiology of FS. In this study, a new strategy for co-immunoprecipitation of insoluble proteins, as well as the standard co-immunoprecipitation method in combination with mass spectrometry and western blot, was employed to explore the relationship between cABYR, AKAP3 and Ropperin. The results showed that AKAP3 was *** with cABYR by the anti-cABYR-A polyclonal antibody, and, conversely, cABYR was also *** with AKAP3 by the anti-AKAP3 polyclonal antibody. Another RIl-like domain containing protein, Ropporin, was also co-immunoprecipitated with cABYR, indicating that Ropporin is one of cABYR＇s binding partners. The interactions between cABYR, AKAP3 and Ropporin were confirmed by yeast two-hybrid assays. Further analysis showed that cABYR not only binds to AKAP3 by its RII domain but binds to Ropporin through other regions besides the RIl-like domain. This is the first demonstration that cABYR variants form a complex not only with the scaffolding protein AKAP3 but also with another Rll-like domain-containing protein in the human sperm FS.

关键词： AKAP3 cABYR fibrous sheath Ropporin sperm tail spermatozoa Western blotting

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人精子纤维鞘多态性蛋白cABYR的寡聚化作用

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第三军医大学学报 2010年第21期32卷 2263-2268页

作者：李彦锋何畏梁培禾张军孙中义张勇王洛夫靳风烁 charles J. Flickinger john c. herr 第三军医大学大坪医院野战外科研究所泌尿外科重庆400042 第三军医大学西南医院妇产科重庆400038 22908USA 美国弗吉尼亚大学细胞生物学系避孕和生殖健康研究中心

目的验证钙结合酪氨酸磷酸化调节蛋白（calcium-binding tyrosine phosphorylation-regulated protein,cABYR）不同异构体之间寡聚体的形成。方法人精子提取物进行非还原和还原条件下的SDS-PAGE、二维IEF/SDS-PAGE、对角线电泳、二维blu... 详细信息

目的验证钙结合酪氨酸磷酸化调节蛋白（calcium-binding tyrosine phosphorylation-regulated protein,cABYR）不同异构体之间寡聚体的形成。方法人精子提取物进行非还原和还原条件下的SDS-PAGE、二维IEF/SDS-PAGE、对角线电泳、二维blue native/SDS-PAGE电泳和免疫印迹分析。结果在人精子中丰度最高的cABYR亚型是相对分子质量86×103仅含cRA编码区表达序列,具有钙结合能力的异构体。同时也存在既含cRA又含cRB编码区表达序列的cABYR亚型。在非还原条件下,丰度最高的130×103二聚体是由仅含cRA表达序列的86×103异构体和既含cRA又含cRB表达序列的50×103异构体组成。（200-220）×103寡聚体是由86×103、50×103异构体,以及35×103（可能既含cRA又含cRB表达序列）的异构体和仅含cRA表达序列的30×103异构体组成。结论证实cABYR异构体可形成寡聚体,cABYR主要通过86×103和50×103异构体形成异质二聚体,与其他cABYR异构体结合进一步形成更大的寡聚体,参与了人精子纤维鞘超分子结构的装配和形成。

关键词：钙结合酪氨酸磷酸化调节蛋白精子纤维鞘寡聚化作用多态性

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FScB蛋白在鼠精子发生过程中的动态表达和迁移研究

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重庆医科大学学报 2009年第9期34卷 1171-1175页

作者：李彦锋何畏张军张克勤梁培禾孙中义刘顺利靳风烁 charles J.Flickinger john c.herr 第三军医大学大坪医院野战外科研究所泌尿外科重庆400042 第三军医大学西南医院妇产科重庆400038 美国弗吉尼亚大学细胞生物学系避孕和生殖健康研究中心弗吉尼亚22908

目的:探讨一种新的睾丸特异性蛋白FScB(Fibrous sheath cABYR binding protein)在精子内的精细动态表达和迁移过程,并研究该蛋白在正常和畸形精子中的形态定位特征。方法:应用过碘酸-希夫氏染色获得鼠睾丸曲精小管上皮中精子发生的时相... 详细信息

目的:探讨一种新的睾丸特异性蛋白FScB(Fibrous sheath cABYR binding protein)在精子内的精细动态表达和迁移过程,并研究该蛋白在正常和畸形精子中的形态定位特征。方法:应用过碘酸-希夫氏染色获得鼠睾丸曲精小管上皮中精子发生的时相性背景特征,然后应用间接免疫荧光方法对FScB蛋白在睾丸和精子内的动态表达和迁移过程进行观察。结果:FScB最初表达于长精细胞的第11步(时相Ⅺ),呈胞浆内弥漫性染色;随之从第11步(时相Ⅺ)至第15步(时相Ⅵ)表达逐渐加强;在精子形成的第15步(时相Ⅳ至时相Ⅵ)FScB开始向精子鞭毛迁移,最终于第16步(时相Ⅷ)FScB蛋白整体局限定位于睾丸精子鞭毛上,胞浆内荧光染色消失。进一步研究显示该蛋白在正常精子中定位于鞭毛主段、精子头部、鞭毛颈段、中段和末段均无表达。在畸形精子中,该蛋白表达存在3种异常改变:①鞭毛内无阳性表达;②表达节段前移并缩短,提示这类精子缺乏中段;③呈现间断分布的串珠状改变。结论:FScB是纤维鞘的重要组成蛋白,在精子纤维鞘形成的后期装配于其表面,可能是纤维鞘生物合成的最后装配步骤。该蛋白在畸形精子中存在明显表达异常,其表达异常与精子运动功能之间的关系有待深入研究。

关键词：精子纤维鞘精子发生睾丸特异性蛋白

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Impact of cOVID-19 pandemic on cardiovascular health in sedentary and athletes:consensus,uncertainties,and ways for mitigation

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Sports Medicine and Health Science 2024年第3期6卷 205-208页

作者： john c.Quindry Lei Xi School of Integrative Physiology and Athletic Training University of MontanaMissoulaMT59812USA Pauley Heart center Department of Internal MedicineSchool of MedicineVirginia Commonwealth UniversityRichmondVA23298-0204USA

More than 4 years has elapsed since the World Health Organization(WHO)ofﬁcially announced the coronavirus disease 2019(cOVID-19)as a pandemic outbreak in March 11,*** the ongoing investigations and political debates upon the origin,exacerbation,resurgence,andﬁnal control of cOVID-19,medical and scientiﬁc consensus indicates that the now endemic cOVID-19 virus is one of the worst public health crises in our *** every person on earth has been directly or indirectly impacted by the adverse effects of the highly contagious viral pathogen-SARS-coV-2(severe acute respiratory syndrome coronavirus 2).Indeed,the population reach of the cOVID-19 infection includes governmental response measures(e.g.,lockdowns and social distancing,mandates for masks and face coverings,vaccines,etc.)that have restricted entrenched societal routines,personal mobility,and ***,the host of health problems linked to this viral infection linger to this day,with selective groups of people experiencing a higher risk for untoward medical outcomes and/or elevated levels of vulnerability to the post-acute sequelae of cOVID-19(***-cOVID).For example,a 2021 study by Logue et *** initial evidence that 10%–30%of individuals in United States may experience prolonged symptoms following SARS-coV-2 infection,including cardiovascular abnormalities.1 Similarly,a 2021 retrospective cohort study in England suggest that the incidence of major adverse cardiovascular events(*** failure,myocardial infarction,ischemic stroke.

关键词： cardiovascular routine Impact

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Review of Networked Microgrid Protection:Architectures,challenges,Solutions,and Future Trends

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cSEE Journal of Power and Energy Systems 2024年第2期10卷 448-467页

作者： Jorge de la cruz Ying Wu john E.candelo-Becerra Juan c.Vasquez Josep M.Guerrero center for Research on Microgrids(cROM) Department of Energy TechnologyAalborg University9220 AalborgDenmark Facultad de Minas Departamento de Energia Eléctrica y AutomaticaUniversidad Nacional de ColombiaSede MedellinCarrera 80 No.65-223RobledoMedellin 050041Colombia. center for Research on Microgrids at UPc(UPc cROM) Department of Electronic EngineeringTechnical University of Catalonia(UPC)08019 Barcelona Spain IcREA Pg.Lluis Companys 2308010 BarcelonaSpain center for Research on Microgrids at AAU(AAU cROM) AAU EnergyAalborg University(AAU)9220 Aalborg EastDenmark

Design and selection of advanced protection schemes have become essential for reliable and secure operation of networked *** protection schemes that allow correct operation of microgrids have been proposed for individual systems in different topologies and ***,protection schemes for networked microgrids are still in devel-opment,and further research is required to design and operate advanced protection in interconnected *** of these microgrids in different nodes with various intercon-nection technologies increases fault occurrence and complicates protection *** paper aims to point out challenges in developing protection for networked microgrids,potential solutions,and research areas that need to be addressed for their ***,this article presents a systematic analysis of different microgrid clusters proposed since 2016,including several architectures of networked microgrids,operation modes,components,and utilization of renewable sources,which have not been widely explored in previous review ***,the paper presents a discussion on protection systems currently available for microgrid clusters,current challenges,and solutions that have been proposed for these ***,it discusses the trend of protection schemes in networked microgrids and presents some conclusions related to ***—Adaptive eprotection,microgrid cluster,microgrid,multiple microgrid,networked microgrid,real-time simulation,smart grid.

关键词： Adaptive eprotection microgrid cluster microgrid multiple microgrid networked microgrid real-time simulation smart grid

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Assessment of clinical depression metrics in cardiac patients using the patient health Questionnaire-9 before and after phase-II cardiac rehabilitation

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Sports Medicine and Health Science 2024年第3期6卷 240-245页

作者： john c.Quindry Michael McNamara carrie Oser crystelle Fogle Integrative Physiology and Athletic Training University of MontanaMissoulaMTUSA St.Patrick's Hospital MissoulaMTUSA cardiovascular Health Program–Montana Department of Public Health and Human Services HelenaMTUSA

cardiovascular disease remains a leading cause of morbidity and mortality,a fact that is commonly associated with co-morbidities such as clinical *** phase II cardiac rehabilitation is an established intervention for those with cardiovascular disease,its effect on patients who also suffer from depression are under ***:To quantify Pre-and Post-cardiac rehabilitation questionnaire scores collected from a large patient data *** this investigation,27670 patients completed Patient Health Questionnaire-9 questionnaires both Pre-and Post-rehabilitation(averaging[28.08.7]phase II sessions).Findings reveal that questionnaire scores decreased by 40%–48%across all groups,a finding that was independent of assigned sex,race,and ***,when data were stratified for questionnaire scores that may indicate major and minor depressive disorder,phase II cardiac rehabilitation outcomes were lower by 61%and 49%*** all groups exhibited lower questionnaire scores following cardiac rehabilitation participation,numerical differences at Preand Post-rehabilitation time points indicate that males and White patients have more favorable *** latter observation,while not confirmed currently,appears to be linked to referral rates to phase II cardiac rehabilitation,which remain poor for females,racial and ethnic minorities.

关键词： clinical depression Exercise Psychological health

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Boron neutron capture therapy of cancer:where do we stand now?

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cancer communications 2024年第8期44卷 889-892页

作者： Rolf F.Barth Gong Wu Maria da Graca H.Vicente john c Grecula Nilendu Gupta Department of Pathology The Ohio State UniversityColumbusOhioUSA Mass Spectrometry and Proteomics Facility The Ohio State UniversityColumbusOhioUSA Department of chemistry Louisiana State UniversityBaton RougeLouisianaUSA Department of Radiation Oncology The Ohio State UniversityColumbusOhioUSA

1 INTRODUcTION,This is the third Editorial/commentary that one of us(***)has written relating to boron neutron capture therapy(BNcT)[1,2].For those readers who are unfamiliar with BNcT we would refer them to several recent comprehensive reviews[3–5].The second Editorial ended on a hopeful note that with the introduction of acceleratorbased neutron sources(ABNSs),BNcT would enter into the mainstream of radiation therapy[2].

关键词： boron 1,2 neutron

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Optimizing oncolytic virus design:a“Swiss army knife”approach to create a systemically delivered therapeutic

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Signal Transduction and Targeted Therapy 2024年第5期9卷 1791-1792页

作者： carolina S.Ilkow john cameron Bell Ottawa Hospital Research Institute 501 Smyth RoadOttawaONK1H 8L6Canada

In a recent paper published in Nature Biotechnology,chen et al.1 describe a multiplex oncolytic virus platform that coupled with adoptive T cell therapy could solve the problem of repeat,systemic delivery of virus based *** is widely accepted that multi-targeted therapeutic approaches attacking several facets of cancer biology are necessary to effectively deal with the tumor heterogeneity that exists within and between patients.

关键词： therapeutic al. targeted

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Hepatic angiotensin-converting enzyme 2 expression in metabolic dysfunction-associated steatotic liver disease and in patients with fatal cOVID-19

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World Journal of Gastroenterology 2024年第31期30卷 3705-3716页

作者： Angus K Jacobs Steven D Morley Kay Samuel Katie Morgan Lyndsey Boswell Timothy J Kendall David A Dorward Jonathan A Fallowfield Peter c Hayes john N Plevris Hepatology Laboratory University of EdinburghEdinburgh EH164SBUnited Kingdom Scottish National Blood Transfusion Service Jack Copland CentreEdinburgh EH144BEUnited Kingdom Institute for Regeneration and Repair University of EdinburghEdinburgh EH164UUUnited Kingdom Edinburgh Pathology University of EdinburghEdinburgh EH164SBUnited Kingdom

BAcKGROUND Metabolic dysfunction-associated steatotic liver disease(MASLD),characterised by hepatic lipid accumulation,causes inflammation and oxidative stress accompanied by cell damage and *** injury(LI)is also frequently reported in patients hospitalised with coronavirus disease 2019(cOVID-19),while preexisting MASLD increases the risk of LI and the development of cOVID-19-associated *** of injury at the cellular level remain unclear,but it may be significant that severe acute respiratory syndrome coronavirus 2(SARS-coV-2)which causes cOVID-19,uses angiotensin-converting expression enzyme 2(AcE2),a key regulator of the‘anti-inflammatory’arm of the renin-angiotensin system,for viral attachment and host cell *** To determine if hepatic AcE2 levels are altered during progression of MASLD and in patients who died with severe *** AcE2 protein levels and localisation,and histological fibrosis and lipid droplet accumulation as markers of MASLD were determined in formalin-fixed liver tissue sections across the MASLD pathological spectrum(isolated hepatocellular steatosis,metabolic dysfunction-associated steatohepatitis(MASH)+/-fibrosis,end-stage cirrhosis)and in post-mortem tissues from patients who had died with severe cOVID-19,using AcE2 immunohistochemistry and haematoxylin and eosin and picrosirius red staining of total collagen and lipid droplet areas,followed by quantification using machine learning-based image pixel *** AcE2 staining is primarily intracellular and concentrated in the cytoplasm of centrilobular hepatocytes and apical membranes of bile duct ***,AcE2 protein levels are elevated in non-fibrotic MASH compared to healthy controls but not in the progression to MASH with fibrosis and in ***2 protein levels and histological fibrosis are not associated,but AcE2 and liver lipid droplet content are significantly correlated across the MASLD *** A

关键词： Metabolic dysfunction-associated steatotic liver disease Angiotensin-converting enzyme 2 Immunohistochemistry cOVID-19 cOVID-19-associated cholangiopathy

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Interfacial friction induced capillary flow within nanofiber-supported ionic liquid droplets

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Green Energy & Environment 2024年第5期9卷 789-791页

作者： Yuanyuan Zhao Gang Xia Yintung Lam john Haozhong Xin School of Fashion and Textiles The Hong Kong Polytechnic UniversityHong KongChina Shenzhen Research Institute The Hong Kong Polytechnic UniversityShenzhenChina

As global economic growth increases,the demand for energy sources *** fossil fuels have traditionally satisﬁed this demand,their environmental inﬂuence and limited reserves require *** fuel combustion contributes substantially to greenhouse gas emissions,with a pressing need to halve these emissions by 2030 and target net-zero by *** energy sources,contributing currently to 29%of global electricity,are viewed as promising *** wind energy's potential,Zheng's team developed a novel method to harness even low wind speeds using well-aligned nanoﬁbers and an innovative“drop wind generator”.This system,combining moisture-saturated ionic liquid 3-Methyl-1-octylimidazolium chloride with speciﬁc nanoﬁber arrays,exploits wind-inducedﬂows for energy *** study highlights the vast untapped potential of low-speed wind as a sustainable energy source potentially for electronics.

关键词： Wind energy Low-speed wind Ionic liquid Electronic devices

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