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Effects of Methanolic and Aqueous Extracts of Griffonia simplicifolia (Fabaceae) on the Inhibition of Falciformation of Human Hb SS Erythrocytes

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Open Journal of Applied Sciences 2023年第1期13卷 24-35页

作者： joel akakpo-akue Gnamien Marcel Ahon Tatiana Kangah Mireille Kplé Loukou Marie Louise Djaha Fofie Yvette Sanogo Ibrahime Bahi Calixte Djaman Allico Joseph Laboratory of Biology and Health UFR Biosciences Université Félix Houphou&euml t-Boigny (UFHB) Abidjan C&ocirc te d’Ivoire National Pedagogical Institute of Technical and Professional Education Abidjan C&ocirc te d’Ivoire Pharmacognosy Laboratory UFR of Pharmaceutical and Biological Sciences Université Félix Houphou&euml t -Boigny Abidjan C&ocirc te d’Ivoire Department of Clinical Haematology University Hospital of Yopougon Abidjan C&ocirc te d’Ivoire Institut Pasteur de Cô te d’Ivoire Abidjan C&ocirc te d’Ivoire

Sickle cell disease (SCD) is a genetic blood disorder that affects the shape and transportation of red blood cells (RBCs) in blood vessels, leading to various clinical complications. Sickle cell disease is a widespread genetic disease in Black Africa. The objective of this study was to evaluate the sickling inhibition activity of Griffonia simplicifolia. Quantitative and qualitative tests were used to determine the major groups of secondary metabolites present in the Griffonia simplicifolia leaves extracts and the modified Emmel test was used to perform the study of sickling inhibition activity. The OECD 423 toxicity study showed that at a single dose of 2000 mg/kg bw, Griffonia simplicifolia leaves extracts are not toxic. All tested substances inhibit erythrocyte falciformation in a dose-dependent manner. The percentages of inhibition were 50.35%, 73% and 94.23% for aqueous extract, hydromethanolic extract and phenylalanine respectively at the concentration of 15 mg/mL. The methanolic extract (70%) had higher activity compared to the aqueous extract. These results suggest that the Griffonia simplicifolia extracts have some potential to be used as alternative antisickling therapy in SCD management.

关键词： Griffonia simplicifolia Antisickling Aqueous Extract Methanolic Extract SS Erythrocyte

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Phytochemical Characterization of Three Plants and Their Antisickling Activity in the Management of Sickle Cell Disease

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Journal of Biosciences and Medicines 2020年第6期8卷 100-112页

作者： Tatiana Kangah Mireille Kplé joel akakpo-akue Julien Koffi Golly Yvette Fofie Marcel Gnamien Ahon Mattieu Adou Kra Ibrahime Sanogo Antoinette Chiayé C. Yapo-Crezoit Laboratory of Biology and Health UFR Biosciences Université Félix Houphou&#235 t-Boigny (UFHB) Abidjan C&#244 te d’Ivoire Immunity Biology Center Institut Pasteur de C&#244 te d’Ivoire Abidjan C&#244 te d’Ivoire UFR of Pharmaceutical and Biological Sciences Université Félix Houphou&#235 t-Boigny (UFHB) Abidjan C&#244 te d’Ivoire Clinical Haematology Department University Hospital of Yopougon Abidjan C&#244 te d’Ivoire

Natural plant products have been used by the population of the south-eastern part of Côte d’Ivoire in the management of sickle cell anemia. This study was aimed at investigating the antisickling activity of the hydro-ethanolic extract of a combination of the leaves of J. secunda, J. gossypiifolia and P. nigrescens. These three plants species were used in the Ivorian traditional herbal medicine. Preliminary phytochemistry was carried out using standard methods. As for the sickling reversal test, the Hb SS blood sickling was induced with 2% sodium metabisulfite. After 120 minutes of incubation, the plant extract was added. Every 30 minutes for a period of 120 minutes, a drop of the prepared solution was observed at (40×) magnification and the percentage of reversion calculated. The phytochemical analysis revealed the presence of Alkaloids, Flavonoids, Polyphenols, Catechic Tannins, Sterols and Polyterpenes. The results of the reversal test showed that the percentage of sickling reversal effect of the combination of the plants (75.00 ± 4.33b,c) was highly superior than the negative control (10.17 ± 0.55d) but was significantly (p J. secunda: 83.50 ± 2.33<

关键词： Sickle Cell Disease Antisickling Activity Hydro-Ethanolic Extract J. gossypiifolia P. nigrescens J. secunda Côte d’Ivoire

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