Effects of Methanolic and Aqueous Extracts of Griffonia simplicifolia (Fabaceae) on the Inhibition of Falciformation of Human Hb SS Erythrocytes

作     者：Joel Akakpo-Akue Gnamien Marcel Ahon Tatiana Kangah Mireille Kplé Loukou Marie Louise Djaha Fofie Yvette Sanogo Ibrahime Bahi Calixte Djaman Allico Joseph Joel Akakpo-Akue;Gnamien Marcel Ahon;Tatiana Kangah Mireille Kplé;Loukou Marie Louise Djaha;Fofie Yvette;Sanogo Ibrahime;Bahi Calixte;Djaman Allico Joseph

作者机构：Laboratory of Biology and Health UFR Biosciences Université Félix Houphouë t-Boigny (UFHB) Abidjan Cô te d’Ivoire National Pedagogical Institute of Technical and Professional Education Abidjan Cô te d’Ivoire Pharmacognosy Laboratory UFR of Pharmaceutical and Biological Sciences Université Félix Houphouë t -Boigny Abidjan Cô te d’Ivoire Department of Clinical Haematology University Hospital of Yopougon Abidjan Cô te d’Ivoire Institut Pasteur de Cô te d’Ivoire Abidjan Cô te d’Ivoire 

出 版 物：《Open Journal of Applied Sciences》 (应用科学（英文）)

年 卷 期：2023年第13卷第1期

页      面：24-35页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Griffonia simplicifolia Antisickling Aqueous Extract Methanolic Extract SS Erythrocyte 

摘      要：Sickle cell disease (SCD) is a genetic blood disorder that affects the shape and transportation of red blood cells (RBCs) in blood vessels, leading to various clinical complications. Sickle cell disease is a widespread genetic disease in Black Africa. The objective of this study was to evaluate the sickling inhibition activity of Griffonia simplicifolia. Quantitative and qualitative tests were used to determine the major groups of secondary metabolites present in the Griffonia simplicifolia leaves extracts and the modified Emmel test was used to perform the study of sickling inhibition activity. The OECD 423 toxicity study showed that at a single dose of 2000 mg/kg bw, Griffonia simplicifolia leaves extracts are not toxic. All tested substances inhibit erythrocyte falciformation in a dose-dependent manner. The percentages of inhibition were 50.35%, 73% and 94.23% for aqueous extract, hydromethanolic extract and phenylalanine respectively at the concentration of 15 mg/mL. The methanolic extract (70%) had higher activity compared to the aqueous extract. These results suggest that the Griffonia simplicifolia extracts have some potential to be used as alternative antisickling therapy in SCD management.