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Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay

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Food Quality and Safety 2022年第4期6卷 597-607页

作者： Jiaxu Xiao Hongfei Yang liangni Qin Jixiang Liang Long Li Xiaohui Fan Dapeng Peng National Reference Laboratory of Veterinary Drug Residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug Residues in Foods Huazhong Agricultural UniversityWuhanChina Wuhan Shangcheng Biotechnology Co. Ltd.WuhanChina Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhenChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human health.Here,this study aims to develop a colloidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on site.Materials and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against FQs.By using a strategy of heterologous coating antigen,different coating antigens are applied to detect FQs.The gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and antibody.Results:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:1024000.The combination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting concentration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A comparative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs residues.Conclusions:The results indicated that the test strip can realize full coverage recognition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.

关键词： fluoroquinolones colloidal gold microwell purification residues

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Flexible ureteroscopic treatment of kidney stones: How do the new laser systems change our concepts?

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Asian Journal of Urology 2024年第2期11卷 156-168页

作者： Simin Yu Linhu Liu Ya Li liang Zhou jixiang Chen Hong Li Kunjie Wang Department of Urology Institute of Urology(Laboratory of Reconstructive Urology)West China HospitalSichuan UniversityChengduSichuanChina

Objective: Flexible ureteroscopy (fURS) has become a widely accepted and effective technique for treating kidney stones. With the development of new laser systems, the fURS approach has evolved significantly. This literature review aims to examine the current state of knowledge on fURS treatment of kidney stones, with a particular focus on the impact of the latest laser technologies on clinical outcomes and patient safety.Methods: We conducted a search of the PubMed/PMC, Web of Science Core Collection, Scopus, Embase (Ovid), and Cochrane Databases for all randomized controlled trial articles on laser lithotripsy in September 2023 without time restriction.Results: We found a total of 22 relevant pieces of literature. Holmium laser has been used for intracavitary laser lithotripsy for nearly 30 years and has become the golden standard for the treatment of urinary stones. However, the existing holmium laser cannot completely powder the stone, and the retropulsion of the stone after the laser emission and the thermal damage to the tissue have caused many problems for clinicians. The introduction of thulium fiber laser and Moses technology brings highly efficient dusting lithotripsy effect through laser innovation, limiting pulse energy and broadening pulse frequency.Conclusion: While the holmium:yttrium-aluminum-garnet laser remains the primary choice for endoscopic laser lithotripsy, recent technological advancements hint at a potential new gold standard. Parameter range, retropulsion effect, laser fiber adaptability, and overall system performance demand comprehensive attention. The ablation efficacy of high-pulse-frequency devices relies on precise targeting, which may pose practical challenges.

关键词： Laser lithotripsy Urolithiasis Thulium laser Holmium:yttrium-aluminum-garnet Moses effect

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The dose regimen formulation of doxycycline hydrochloride and florfenicol injection based on ex vivo pharmacokinetic-pharmacodynamic modeling against the Actinobacillus pleuropneumoniae in pigs

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Animal Diseases 2023年第4期3卷 286-298页

作者： Yuanyuan Yuan Boyu An Shuyu Xie Wei Qu Haihong Hao Lingli Huang Wanhe Luo Jixiang Liang Dapeng Peng National Reference Laboratory of Veterinary Drug Residues(HZAU)and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods Huazhong Agricultural UniversityWuhanHubei 430070People's Republic of China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhanHubei 430070People's Republic of China College of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070HubeiPeople's Republic of China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityWuhan 430070HubeiPeople's Republic of China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenPeople's Republic of China

Doxycycline hydrochloride and florfenicol combination(DoxHcl&FF)is an effective treatment for respiratory diseases.In the study,our objective Was to evaluate the activity of DoxHcl&FF against Actinobacillus pleuropneumoniae(APP)in porcine pulmonary epithelial lining fluid(PELF)and the optimal dosage scheme to avoid the development of resistance.The DoxHcl&FF Was administered intramuscularly(IM)at 20mg/kg,and the PELF was collected at differ-ent time points.The minimum inhibitory concentration(MIC)and time-mortality curves were also included in the study.Based on the sigmoid Emax equation and dose equations,the study integrated the in vivo pharmacokinetic data of infected pigs and ex vivo pharmacodynamic data to obtain the area under concentration time curve(AUCo-24h)MIC values in PELF and achieve bacteriostatic activity,bactericidal activity and the virtual eradication of bacteria.The study showed that the combination of DoxHcl and FF caused no significant changes in PK parameters.The peak concentration(Cmax)of FF in healthy and diseased pigs was 8.87±0.08 and 8.67±0.07μg/mL,the_AUCo-24h were.172.75±2.52 and 18022±3.13 h-μg/mL,the Cmax of DoxHcl was 7.91±0.09 and 7.99±0.05μg/mL,and the AUCo-24h was 129.96±3.70 h-μg/mL and 169.82±4.38 h-μg/mL.DoxHcl&FF showed strong concentra-tion-dependent tendencies.The bacteriostatic,bactericidal,and elimination activity were calculated as 5.61,18.83 and 32.68 h,and the doses were 1.37(bacteriostatic),4.59(bactericidal)and 7.99(elimination)mg/kg.These findings indicated that the calculated recommended dose could assist in achieving more precise administration,increasing the effectiveness of DoxHcl&FF treatment for APP infections.

关键词： Florfenicol Doxycycline hydrochloride PK-PD Pig Dose regimen Actinobacillus pleuropneumoniae

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The strength and function of hip abductors following anterolateral minimally invasive total hip arthroplasty

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Chinese Journal of Traumatology 2014年第2期17卷 73-78页

作者： Tan jixiang Chen Hong Chen Cheng liang Xi Huang Wei Department of Emergency & Critical Care Medicine First Affiliated Hospital Chongqing Medical UniversityChongqing 400016 China Department of Orthopedic Department First AffiliatedHospital Chongqing Medical University Chongqing400016 China

Objective:To analyze the extent of postoperative hip abductor insufficiency in primary total hip arthroplasty (THA) patients undergoing anterolateral minimally invasive (ALM1) approach, and to investigate whether the clinical outcomes are more favorable in femoral neck fracture (FNF) patients than in non-femoral neck fracture (nFNF) patients. Methods:A total of 48 patients were enrolled in this study. Each patient underwent a clinical examination preoperatively and 6, 12, 24 and 48 weeks postoperatively. The abductor torque, Trendelenburg's sign, gait velocity, Harris hip score, Oxford hip score, Westren Ontario and McMaster Universities (WOMAC) score and visual analog scale pain score were recorded. Statistical evaluation was performed with SPSS software version 18.0. The significance level was set at P<0.05. Results:The abductor torque of the operated hip and the recovery ratio showed a gradual improving tendency from 6 weeks postoperatively until the last follow-up. Gait velocity, Harris hip score, Oxford hip score and WOMAC score improved significantly after the operation until 24 weeks postoperatively. In the FNF group, the abductor torque of the operated side and the recovery ratio were significantly higher than in nFNF group at 6 weeks postoperatively, however, as time passed, this trend tended to disappear. Conclusion:This study demonstrates that patients can obtain good abductor strength and function in the early postoperative period and the hip abductor function of patients who suffer from hip osteoarthritis, rheumatoid arthritis, avascular necrosis of the femoral head could be significantly improved following ALMI THA.

关键词：髋关节肌力置换 Harris 类风湿性关节炎强度临床检查骨性关节炎

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Simulation of the neck growth of non-isometric biosphere during initial sintering

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Acta Metallurgica Sinica(English Letters) 2009年第4期22卷 263-274页

作者： jixiang DU Shuhua liang Xianhui WANG Zhikang FAN Department of Material Science ＆ Engineering Xi＇an University of Technology Xi＇an 710048 China

Because powders are mostly non-isometric during the sintering process, copper powders were chosen to study the effects of four material transport mechanisms, including surface diffusion, grain-boundary diffusion, volume diffusion, and multi-couplings. These material transport mechanisms were studied with respect to sintering neck growth of a non-isometric biosphere during initial sintering. The evolution of the neck growth in the four transport mechanisms was simulated by Visual C＋＋ as well based on the model of different particles. The results show that the increase of the sintering temperature, both the grain-boundary diffusion and volume diffusion play primary roles in neck growth, while surface diffusion gradually becomes the secondary mechanism. Both the sintered neck and the shrinkage of the two centers increase with increasing temperature by means of the coupling diffusion mechanism. The radius of the sintering neck decreased, and the shrinkage rate of the two centers increased with an increase of the diameter ratio of the two spheres.

关键词： Sintering simulation Non-isometric biosphere model Diffusion mechanism Sintering neck

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Electrocatalytic biofilm reactor for effective and energyefficient azo dye degradation:the synergistic effect of MnO_(x)/Ti flow-through anode and biofilm on the cathode

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Frontiers of Environmental Science & Engineering 2023年第4期17卷 127-141页

作者： Yinghui Mo Liping Sun Lu Zhang Jianxin Li jixiang Li Xiuru Chu liang Wang State Key Laboratory of Separation Membranes and Membrane Processes National Center for International Joint Research on Membrane Science and TechnologyTiangong UniversityTianjin 300387China School of Environmental Science and Engineering Tiangong UniversityTianjin 300387China School of Materials Science and Engineering Tiangong UniversityTianjin 300387China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 200120China University of Chinese Academy of Sciences Beijing 100049China Tianjin Water Engineering Co. Ltd.Tianjin 300222China

Dyeing wastewater treatment remains a challenge.Although effective,the in-series process using electrochemical oxidation as the pre-or post-treatment of biodegradation is long.This study proposes a compact dual-chamber electrocatalytic biofilm reactor(ECBR)to complete azo dye decolorization and mineralization in a single unit via anodic oxidation on a MnO_(x)/Ti flow-through anode followed by cathodic biodegradation on carbon felts.Compared with the electrocatalytic reactor with a stainlesssteel cathode(ECR-SS)and the biofilm reactor(BR),the ECBR increased the chemical oxygen demand(COD)removal efficiency by 24%and 31%(600 mg/L Acid Orange 7 as the feed,current of 6 mA),respectively.The COD removal efficiency of the ECBR was even higher than the sum of those of ECR-SS and BR.The ECBR also reduced the energy consumption(3.07 kWh/kg COD)by approximately half compared with ECR-SS.The advantages of the ECBR in azo dye removal were attributed to the synergistic effect of the MnO_(x)/Ti flow-through anode and cathodic biofilms.Catalyzed by MnIV=O generated on the MnO_(x)/Ti anode under a low applied current,azo dyes were oxidized and decolored.The intermediate products with improved biodegradability were further mineralized by the cathodic aerobic heterotrophic bacteria(non-electrochemically active)under the stimulation of the applied current.Taking advantage of the mutual interactions among the electricity,anode,and bacteria,this study provides a novel and compact process for the effective and energyefficient treatment of azo dye wastewater.

关键词： Azo dye removal Electrocatalytic biofilm reactor Anodic oxidation Electricity-stimulated biodegradation Energy consumption

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一种利用平面绕轴转动进行轴标定的方法

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计算机应用与软件 2024年第5期41卷 107-112页

作者：徐杰李振亮梁吉祥北京城市学院3D打印研究院北京100083

提出一种基于平面绕轴转动计算转轴直线方程的方法。分别讨论了多平面方程组的秩为3和秩为2两种情况的解法。除了秩为1的情形外,该方法适用于大部分的转轴标定情况。实验表明转轴标定误差与平面方程系数的误差大小成正比,标定精度优于... 详细信息

提出一种基于平面绕轴转动计算转轴直线方程的方法。分别讨论了多平面方程组的秩为3和秩为2两种情况的解法。除了秩为1的情形外,该方法适用于大部分的转轴标定情况。实验表明转轴标定误差与平面方程系数的误差大小成正比,标定精度优于基于空间点绕轴转动方法的标定精度。

关键词：转轴标定平面转动

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中晚期原发性肝癌患者经导管肝动脉化疗栓塞术前后血清抗Survivin抗体变化及其与患者预后的关系

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中华普通外科杂志 2023年第9期38卷 669-673页

作者：李冠群梁超杰伍冀湘首都医科大学附属北京同仁医院普外科北京100730 山西医科大学第一医院肝胆胰外科太原030001

目的探讨中晚期原发性肝癌患者经导管肝动脉化疗栓塞术(transcatheter hepatic arterial chemoembolization,TACE)前后血清抗Survivin抗体变化及其与患者预后的关系。方法选择同仁医院收治的中晚期原发性肝癌患者103例,均行TACE术治疗,... 详细信息

目的探讨中晚期原发性肝癌患者经导管肝动脉化疗栓塞术(transcatheter hepatic arterial chemoembolization,TACE)前后血清抗Survivin抗体变化及其与患者预后的关系。方法选择同仁医院收治的中晚期原发性肝癌患者103例,均行TACE术治疗,检测患者术前1 d、术后1个月血清抗Survivin抗体表达水平。分析血清抗Survivin抗体水平变化与患者肿瘤近期治疗疗效、临床病理特征及预后的关系。结果疾病缓解患者术后抗Survivin抗体水平显著低于疾病未缓解患者(81.84±9.30比90.84±10.21,P<0.05),疾病缓解患者抗Survivin抗体变化显著高于疾病未缓解患者(30.93±5.63比22.75±4.52,P<0.05)。TACE手术前后,抗Survivin抗体变化与患者TNM分期、肿瘤最大径及分化程度有关(P<0.05)。生存情况分析结果显示,△抗Survivin抗体减少患者术后生存情况显著优于△抗Survivin抗体未减少患者(P<0.05)。△抗Survivin抗体预测原发性肝癌患者1年内死亡的价值,ROC曲线下面积为0.850。结论中晚期肝癌患者TACE手术前后抗Survivin抗体变化差异与患者近远期疗效有关。

关键词：肝肿瘤化学栓塞,治疗性抗凋亡抑制因子抗体预后

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腹腔镜结肠切除术在轻症结直肠穿孔诊治中的应用

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实用医学杂志 2018年第12期34卷 2039-2041页

作者：彭吉祥梁顺宇广州市第一人民医院胃肠外科广州510180

目的探讨轻症结直肠穿孔患者行腹腔镜病变肠段切除治疗的可行性。方法回顾性研究我院2006年6月至2016年6月间收治的61例病变肠段切除治疗的轻症结直肠穿孔患者的临床资料,对开腹手术组与腹腔镜手术组患者的术中及术后临床指标进行比较... 详细信息

目的探讨轻症结直肠穿孔患者行腹腔镜病变肠段切除治疗的可行性。方法回顾性研究我院2006年6月至2016年6月间收治的61例病变肠段切除治疗的轻症结直肠穿孔患者的临床资料,对开腹手术组与腹腔镜手术组患者的术中及术后临床指标进行比较分析。结果腹腔镜手术组相较开腹手术组,术中失血量相对较少[(100±165)m L vs.(200±205)m L,P=0.032],术后住院时间短[(7±5.21)d vs.(10±9.2)d,P=0.009],但手术时间偏长[(213.7±65.7)min vs.(173.5±47.2)min,P=0.047];开腹组与腹腔镜组在术中输血量[(0.8±1.1)U vs.(0.4±0.7)U,P=0.270]、术后ICU监护时间[(2±0.9)d vs.(2±0.7)d,P=0.009]、术后并发症(25/38 vs.14/23,P=0.70)、住院费用[(31 758±29 283)元vs.(36243±27 584)元,P=0.673]均未有明显差异。结论轻症结直肠穿孔患者选择性的采用腹腔镜肠切除是安全可行的。

关键词：结直肠穿孔腹腔镜结肠切除

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Coexistence of metamagnetism and single chain magnet behavior in a Fe^Ⅲ_2Co^Ⅱ layer compound

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Science China Chemistry 2016年第6期59卷 735-739页

作者： Wenjing Jiang Yanjuan Zhang Chengqi Jiao liang Zhao jixiang Hu Junli Wang Tao Liu State Key Laboratory of Fine Chemicals Dalian University of Technology Dalian 116012 China

A cyanide-bridged FeⅢ2CoⅡ double zigzag chain, {[FeⅢ（bipy）（CN）4]2Co～Ⅱ（btab）2}n [bipy=2,2′-bipyridine, btab=4,4′-（1,3-phenylene）-bis-4H-1,2,4-triazole]（1）, was obtained with tetracyanometalate precursors and CoII ions. The chains were further linked by the ditopic btab ligands to a layer. Magnetic property studies demonstrate that 1 shows both metamagnetism with a critical field Hc=400 Oe and single-chain magnet behavior.

关键词：磁性转变单链行为磁体化合物共存氰根桥联 bipy

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