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微生物菌肥对油茶生长、抗逆性和土壤环境的影响

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世界林业研究 2024年第3期37卷 28-32页

作者：舒惠琳胡文涛陈启洲陈惠珊陈倩虹黄久香黄永芳潘澜华南农业大学林学与风景园林学院广州510642

微生物菌肥是一种环保、低碳、无污染的有机生物肥料,可通过增加土壤中微生物的数量和活性调整养分平衡,有效改善土壤生态环境,进而促进植物生长、增加产量和提高果实品质。文中阐述了微生物菌肥的促生抗逆作用机理,明确指出菌肥能够有... 详细信息

微生物菌肥是一种环保、低碳、无污染的有机生物肥料,可通过增加土壤中微生物的数量和活性调整养分平衡,有效改善土壤生态环境,进而促进植物生长、增加产量和提高果实品质。文中阐述了微生物菌肥的促生抗逆作用机理,明确指出菌肥能够有效促进土壤微生物活性、改善土壤理化性质、改善植物营养状况和增强植物光合作用,从而促进油茶的生长和养分吸收,提高产量及品质,并有效提高油茶抗旱能力,抑制病原菌的生长。总结微生物菌肥在油茶上的应用前景以及重点研究方向,旨在更好地推动微生物菌肥在油茶上的应用,为提高油茶品质和产量奠定基础。

关键词：油茶微生物菌肥土壤性质生理生态植物抗性

Phylogenetic estimation and morphological evolution of Alsineae(Caryophyllaceae)shed new insight into the taxonomic status of the genus Pseudocerastium

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Plant Diversity 2021年第4期43卷 299-307页

作者： Gang Yao Bine Xue Kun Liu Yuling Li jiuxiang huang Junwen Zhai College of Forestry and Landscape Architecture South China Agricultural UniversityGuangzhou510642China College of Horticulture and Landscape Architecture Zhongkai University of Agriculture and EngineeringGuangzhou510225GuangdongChina College of Life Sciences Anhui Normal UniversityWuhu241000AnhuiChina College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou350002FujianChina

Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to *** genus has been widely accepted since it was described in 1998,however its phylogenetic position within Caryophyllaceae has never been *** the present study,the whole plastid genome and nuclear ribosomal internal transcribed spacer(ITS)sequences of Pseudocerastium stellarioides was obtained through genome skimming,and the phylogenetic position of the species was studied for the first *** phylogenomic analysis of Caryophyllaceae revealed that Pseudocerastium is clustered within the tribe Alsineae with strong *** analyses based on an enlarged taxon sampling of Alsineae using five DNA regions(matK,rbcL,rps16 intron,trnL-F and ITS)revealed that *** was nested deeply within Cerastium with strong *** of morphological character evolution suggest that the ancestral states in Alsineae include three styles and a six-lobed capsule at the apex,while both Cerastium and Pseudocerastium have five styles and ten lobes at the apex of the capsule,further supporting their close *** species Pseudocerastium stellarioides is similar to Cerastium wilsonii in morphology,but differs in having villous indumentum on the lower part of the filaments and compressed globose ***,based on the present molecular and morphological evidence,the generic name Pseudocerastium is reduced here as a new synonym of Cerastium and the species *** is transferred to Cerastium as ***.

关键词： Alsineae Cerastium Character evolution Genome skimming Molecular phylogeny Taxonomy

Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts

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Journal of Genetics and Genomics 2024年第2期51卷 222-229页

作者： Wenbin huang Shiying Zhang jiuxiang Lin Yi Ding Nan Jiang Jieni Zhang Huaxiang Zhao Feng Chen Department of Orthodontics Peking University School and Hospital of StomatologyBeijing100081China Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’anShaanxi710004China Guangdong Provincial High-level Clinical Key Specialty Guangdong Province Engineering Research Center of Oral Disease Diagnosis and TreatmentDepartment of OrthodonticsStomatological CenterPeking University Shenzhen HospitalShenzhen Peking University-The Hong Kong University of Science and Technology Medical CenterShenzhenGuangdong 518036China Department of Physiology and Pathophysiology School of Basic Medical SciencesXi'an Jiaotong UniversityXi'anShaanxi710049China National Center for Stomatology National Clinical Research Center for Oral DiseasesNational Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory for Digital StomatologyResearch Center of Engineering and Technology for Computerized Dentistry Ministry of HealthNMPA Key Laboratory for Dental MaterialsBeijing100101China Central Laboratory Peking University School and Hospital of StomatologyBeijing100081China

Orofacial clefts (OFCs) are the most common congenital craniofacial disorders, of which the etiology is closely related to rare coding variants. Filamin B (FLNB) is an actin-binding protein implicated in bone formation. FLNB mutations have been identified in several types of syndromic OFCs and previous studies suggest a role of FLNB in the onset of non-syndromic OFCs (NSOFCs). Here, we report two rare heterozygous variants (p.P441T and p.G565R) in FLNB in two unrelated hereditary families with NSOFCs. Bioinformatics analysis suggests that both variants may disrupt the function of FLNB. In mammalian cells, p.P441T and p.G565R variants are less potent to induce cell stretches than wild type FLNB, suggesting that they are loss-of-function mutations. Immunohistochemistry analysis demonstrates that FLNB is abundantly expressed during palatal development. Importantly, Flnb^(−/−) embryos display cleft palates and previously defined skeletal defects. Taken together, our findings reveal that FLNB is required for development of palates in mice and FLNB is a bona fide causal gene for NSOFCs in humans.

关键词： Or ofacial clefts FLNB Loss-of-function mutati on Cleft palate Filamin B Actin flannent Knockout mouse

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白塞病肾病：附8例临床分析

中华肾脏病杂志 1989年第5期5卷 302-303页

作者：黄久香邱志亮内蒙古自治区医院江苏南通市第一人民医院

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海南岛青梅AFLP标记的遗传多样性

林业科学 2008年第5期44卷 46-52页

作者：黄久香黄妃本许涵李意德庄雪影华南农业大学林学院广州510642 中国林业科学研究院热带林业研究所广州510520

采用AFLP标记检测海南岛青梅7个主要自然居群192株个体的遗传多样性及遗传结构。应用筛选出的6对引物共扩增出频率大于5.00%的位点777个,其中多态位点比例为99.61%(774)。在种级水平上,Nei’s基因多样性指数(H)为0.2661,Shannon多态性... 详细信息

采用AFLP标记检测海南岛青梅7个主要自然居群192株个体的遗传多样性及遗传结构。应用筛选出的6对引物共扩增出频率大于5.00%的位点777个,其中多态位点比例为99.61%(774)。在种级水平上,Nei’s基因多样性指数(H)为0.2661,Shannon多态性信息指数(I)为0.4204;总基因多样度(Ht)为0.2684。居群水平的平均多态性位点数和多态性比例为617个和79.45%;H和I平均值分别为0.2153和0.3352。遗传分化指数(Fst)和基因流(Nm)分别为0.1980和1.0127。UPGMA聚类和遗传距离与空间距离相关性分析结果表明,居群间遗传亲缘关系与其地理位置关系不明显(P=0.2300,r2=0.2325)。综合研究结果表明,青梅具有较高的遗传多样性,居群间有一定的遗传分化。建议采取人工促进天然林下层苗木生长和人工造林等措施,促进现有青梅居群遗传多样性保育和发展。

关键词：青梅 AFLP 遗传多样性遗传分化保育措施

基于多个叶绿体基因序列片段重建广义苋科系统发育关系

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植物学报 2020年第4期55卷 457-467页

作者：黄久香陈文娜李玉玲姚纲华南农业大学林学与风景园林学院中国南方石灰岩植物研究中心广州510642 河北工程大学园林与生态工程学院邯郸056038

苋科(Amaranthaceaesensulato)是石竹目(Caryophyllales)第二大科,目前被普遍接受的苋科为其广义概念,含狭义苋科(Amaranthaceae sensu stricto)和藜科(Chenopodiaceae)。然而到目前为止,藜科是否应作为独立的科还存在争议。此外,广义苋科内部各亚科之间的系统关系也尚未厘清。对广义苋科所有13个亚科代表类群进行取样(共59种),基于8个叶绿体序列片段重建其系统发育关系,并结合分子钟估算,对该科及其主要分支的起源与分化时间进行推测。结果表明,广义苋科与狭义苋科都是很好的单系,但藜科并非单系,因此不支持藜科在科级水平的地位,支持广义苋科的观点。除了多节草亚科(Polycnemoideae)之外,其它亚科的系统位置均得到很好的分辨。分子钟估算结果表明,广义苋科于白垩纪晚期约69.9Ma分化出该科的2个主要分支,且该科在白垩纪-古近纪边界附近时期(约66.0Ma)可能发生过快速辐射分化事件。

关键词：苋科石竹目分化时间系统发育

A thermodynamic perspective on electrode poisoning in solid oxide fuel cells

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International Journal of Minerals,Metallurgy and Materials 2024年第6期31卷 1449-1455页

作者： Kevin huang Department of Mechanical Engineering University of South CarolinaColumbiaUSA

A critical challenge to the commercialization of clean and high-efficiency solid oxide fuel cell(SOFC)technology is the insuf-ficient stack lifespan caused by a variety of degradation mechanisms,which are associated with cell components and chemical *** components related degradation refers to thermal/chemical/electrochemical deterioration of cell materials under operating conditions,whereas the latter regards impurities in feedstocks of oxidant(air)and reductant(fuel).This article provides a thermodynamic perspective on the understanding of the impurities-induced degradation mechanisms in *** discussion focuses on using thermodynamic ana-lysis to elucidate poisoning mechanisms in cathodes by impurity species such as Cr,CO_(2),H_(2)O,and SO_(2) and in the anode by species such as S(or H_(2)S),SiO_(2),and P_(2)(or PH_(3)).The author hopes the presented fundamental insights can provide a theoretical foundation for search-ing for better technical solutions to address the critical degradation challenges.

关键词： impurities air fuel reaction activity

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青梅ISSR标记的遗传多样性分析

广东林业科技 2015年第3期31卷 40-45页

作者：黄妃本黄久香庄雪影广东省岭南综合勘察设计院广东广州510520 华南农业大学林学与风景园林学院广东广州510642

采用ISSR分子标记检测海南岛青梅(Vatica mangachapoi)7个自然居群192株个体的遗传多样性及遗传结构。筛选的10条ISSR引物共扩增出清晰位点132个,其中多态性位点130个,多态性百分率(PPB)达98.48%。物种水平上,Nei's基因多样性指数(H)为0... 详细信息

采用ISSR分子标记检测海南岛青梅(Vatica mangachapoi)7个自然居群192株个体的遗传多样性及遗传结构。筛选的10条ISSR引物共扩增出清晰位点132个,其中多态性位点130个,多态性百分率(PPB)达98.48%。物种水平上,Nei's基因多样性指数(H)为0.339 1,Shannon多态性信息指数(I)为0.509 5,总基因多样度(Ht)为0.344 1,反映了较高的遗传多样性。AMOVA分析显示,青梅的遗传变异主要存在于居群内个体间,但亦产生了相当程度的居群间遗传分化,Nei′s基因分化系数Gst为0.332 9。Mantel检测表明,青梅的遗传距离与地理距离间没有显著相关性(r2<0.2),地理隔离对青梅各自然居群间的基因流影响不明显。

关键词：青梅 ISSR 遗传多样性遗传分化基因流

Fast Blood Oxygenation through Hemocompatible Asymmetric Polymer of Intrinsic Microporosity Membranes

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Research 2024年第1期2023卷 261-270页

作者： Xinxi huang Junping huang Pengcheng Su Wanbin Li Guangdong Key Laboratory of Environmental Pollution and Health School of EnvironmentJinan UniversityGuangzhou 511443China

Membrane technology has attracted considerable attention for chemical and medical applications,among *** organs play important roles in medical science.A membrane oxygenator,also known as artificial lung,can replenish O_(2) and remove CO_(2) of blood to maintain the metabolism of patients with cardiopulmonary ***,the membrane,a key component,is subjected to inferior gas transport property,leakage propensity,and insufficient *** this study,we report efficient blood oxygenation by using an asymmetric nanoporous membrane that is fabricated using the classic nonsolvent-induced phase separation method for polymer of intrinsic *** intrinsic superhydrophobic nanopores and asymmetric configuration endow the membrane with water impermeability and gas ultrapermeability,up to 3,500 and 1,100 gas permeation units for CO_(2) and O_(2),***,the rational hydrophobic–hydrophilic nature,electronegativity,and smoothness of the surface enable the substantially restricted protein adsorption,platelet adhesion and activation,hemolysis,and thrombosis for the ***,during blood oxygenation,the asymmetric nanoporous membrane shows no thrombus formation and plasma leakage and exhibits fast O_(2) and CO_(2) transport processes with exchange rates of 20 to 60 and 100 to 350 ml m^(−2) min^(−1),respectively,which are 2 to 6 times higher than those of conventional *** concepts reported here offer an alternative route to fabricate high-performance membranes and expand the possibilities of nanoporous materials for membrane-based artificial organs.

关键词： porosity asymmetric Oxygen