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Identification and Fine Mapping of rhm1 Locus for Resistance to Southern Corn Leaf Blight in Maize

Journal of Integrative Plant Biology 2012年第5期54卷 321-329页

作者： Yuanzeng zhao Xiaomin Lu Chaoxian Liu Haiying Guan Mei Zhang Zhongfeng Li Hongwei Cai jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Department of Life Science and Technology Henan Institute of Science and TechnologyXinxiang 453003China Henan Academy of Agricultural Sciences Zhengzhou 450002China

rhml is a major recessive disease resistance locus for Southern corn leaf blight （SCLB）. To further narrow down its genetic position, F2 population and BCIFI population derived from the cross between resistant （H95rhm） and susceptible parents （H95） of maize （Zea mays） were constructed. Using newly developed markers, rhml was initially delimited within an interval of 2.5 Mb, and then finally mapped to a 8.56 kb interval between InDel marker IDP961-503 and simple sequence repeat （SSR） marker A194149--1. Three polymorphic markers IDP961-504, IDP B2-3 and A194149-2 were shown to be co-segregated with the rhml locus. Sequence analysis of the 8.56 kb DNA fragment revealed that it contained only one putative gene with a predicted amino acid sequence identical to lysine histidine transporter 1 （LHT1）. Comparative sequence analysis indicated that the LHT1 in H95rhrn harbors a 354 bp insertion in its third exon as compared with that of susceptible alleles in B73, H95 and Mo17. The 354 bp insertion resulted in a truncation of the predicted protein of candidate resistance allele （LHT1-H95rhm）. Our results strongly suggest LHTI as the candidate gene for rhml against SCLB. The tightly linked molecular markers developed in this study can be directly used for molecular breeding of resistance to Southern corn leaf blight in maize.

关键词： Bipolaris maydis fine-mapping maize rhml Southern corn leaf blight Zea mays.

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Defective Kernel 39 encodes a PPR protein required for seed development in maize

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Journal of Integrative Plant Biology 2018年第1期60卷 45-64页

作者： Xiaojie Li Wei Gu Silong Sun Zongliang Chen Jing Chen Weibin Song Haiming zhao jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural University

RNA editing is a posttranscriptional process that is important in mitochondria and plastids of higher plants. All RNA editing-specific trans-factors reported so far belong to PLS-class of pentatricopeptide repeat(PPR)proteins. Here, we report the map-based cloning and molecular characterization of a defective kernel mutant dek39 in maize. Loss of Dek39 function leads to delayed embryogenesis and endosperm development, reduced kernel size, and seedling lethality. Dek39 encodes an E subclass PPR protein that targets to both mitochondria and chloroplasts, and is involved in RNA editing in mitochondrial NADH dehydrogenase3(nad3) at nad3-247 and nad3-275. C-to-U editing of nad3-275 is not conserved and even lost in Arabidopsis, consistent with the idea that no close DEK39 homologs are present in Arabidopsis. However, the amino acids generated by editing nad3-247 and nad3-275 are highly conserved in many other plant species, and the reductions of editing at these two sites decrease the activity of mitochondria NADH dehydrogenase complex I,indicating that the alteration of amino acid sequence is necessary for Nad3 function. Our results indicate that Dek39 encodes an E sub-class PPR protein that is involved in RNA editing of multiple sites and is necessary for seed development of maize.

关键词： PPR Defective Kernel 39 encodes a PPR protein required for seed development in maize

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Genome-wide Nucleosome Occupancy and Organization Modulates the Plasticity of Gene Transcriptional Status in Maize

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Molecular Plant 2017年第7期10卷 962-974页

作者： Jian Chen En Li Xiangbo Zhang Xiaomei Dong Lei Lei Weibin Song Haiming zhao jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and Breeding China Agricultural UniversityBeijing 100193 P. R. China

Nucleosomes are fundamental units of chromatin that play critical roles in gene regulation by modulating DNA accessibility. However, their roles in regulating tissue-specific gene transcription are poorly understood. Here, we present genome-wide nucleosome maps of maize shoot and endosperm generated by sequencing the micrococcal nuclease digested nucleosomal DNA. The changes of gene transcriptional status between shoot and endosperm were accompanied by preferential nucleosome loss from the promoters and shifts in the first nucleosome downstream of the transcriptional start sites （＋1 nucleosome） and upstream of transcriptional termination sites （-1 nucleosome）. Intrinsically DNA-encoded nucleosome orga- nization was largely associated with the capacity of a gene to alter its transcriptional status among different tissues. Compared with tissue-specific genes, constitutively expressed genes showed more pronounced 5＇ and 3＇ nucleosome-depleted regions as well as further ＋1 nucleosome to transcriptional start sites and -1 nucleosome to transcriptional termination sites. Moreover, nucleosome organization was more highly correlated with the plasticity of gene transcriptional status than with its expression level when examined using in vivo and predicted nucleosome data. In addition, the translational efficiencies of tissue-specific genes appeared to be greater than those of constitutively expressed genes. Taken together, our results indicate that intrinsically DNA-encoded nucleosome organization is important, beyond its role in regulating gene expression levels, in determining the plasticity of gene transcriptional status.

关键词： Nucleosome Gene transcription Plasticity Maize

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Clopidogrel loading dose versus maintenance dose to treat patients with acute ischaemic stroke in China(CLASS-China):results from a prospective double-blind randomised clinical trial

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Stroke & Vascular Neurology 2017年第3期2卷 118-123页

作者： Ying zhao Wanyong Yang Zefeng Tan Wenmin Wang Weimin Xiao jinsheng Zeng Anding Xu Department of Neurology and Stroke Center The First Affiliated HospitalJinan UniversityGuangzhouChina Department of Neurology The First Affiliated HospitalKunming Medical CollegeKunmingYunnanChina Department of Neurology Dongguan People HospitalDongguanGuangdongChina Department of Neurology and Stroke Center First Affiliated HospitalSun Yat-Sen UniversityGuangzhouGuangdongChina

Aim The role of clopidogrel in treating patients with acute ischaemic stroke is *** have conducted the clinical trial in order to evaluate the efficacy and safety of clopidogrel with a loading dose in treating patients with non-cardiogenic acute ischaemic *** Clopidogrel loading dose versus maintenance dose to treat patients with acute ischaemic stroke in China(CLASS-China)was a prospective,randomised,double-blind and placebo-controlled clinical trial in *** with acute ischaemic stroke of non-cardiogenic origin within 48 hours of onset were enrolled and those received thrombolysis were *** patients were divided into two treatment groups:loading dose and routine *** primary outcome was the incidence of stroke recurrence or progression within 7 *** safety outcome was measured by life-threatening *** intent-to-treat analysis was used for the statistical *** From March 2008 to March 2010,a total of 303 patients from 16 centres were recruited into this study;six were excluded because of lack of basic *** the enrolment was slow and the study drug expired in March 2010,this clinical trial was stopped earlier than *** significant baseline and demographic differences were seen between the two *** was no difference in primary outcome between the loading dosage group 16.1%(24/149)and control group 14.9%(22/148),respectively(p=0.782).The mortality and disability rate within 90 days in loading dose group(19.6%)was slightly lower than that in controlled group(23.4%),p=*** dose group had two(1.3%)cases of fatal haemorrhage and control group had four(2.7%)within 90 days,p=*** significant difference was detected in other adverse events between the *** In our study stopped early due to slow enrolment,loading dose of clopidogrel does not reduce the risk of recurrent *** trials with sufficient number of patients enrolled are needed to re-e

关键词： patients dosage routine

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OS1 functions in the allocation of nutrients between the endosperm and embryo in maize seeds

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Journal of Integrative Plant Biology 2019年第6期61卷 706-727页

作者： Weibin Song Jinjie Zhu Haiming zhao Yingnan Li Jiangtao Liu Xiangbo Zhang Liangliang Huang jinsheng Lai State Key Laboratory of Agrobiotechnology and National Maize Improvement Center Department of Plant Genetics and Breeding ChinaAgricultural University

Uncovering the genetic basis of seed develop-ment will provide useful tools for improving both crop yield and nutritional value, However, the genetic regulatory networks of maize (Zea mays) seed development remain largely unknown. The maize opaque endosperm and small germ 1 (osl) mutant has opaque endosperm and a small embryo,Here, we cloned OSi1 and show that it encodes a putative transcription factor containing an RWP-RK domain-Transcriptional analysis indicated that OSi1 expression is elevated in early endosperm development, especially in the basal endosperm transfer layer (BETL), conducting zone (CZ), and central starch endospcrm (CSE) cells. RNA sequencing (RNA-Seq) analysis of the osi mutant revealed sharp downregulation of certain genes in specific cell types, including ZmMRP-1 and Megl in BETL cells and a majority of zein-and starch-related genes in CSE cells. Using a haploid induction system, we show that wild-type endosperm could rescue the smaller size of osi embryo, which suggests that nutrients are allocated by tho wild type endosperm. Therefore, our dsta imply that the network regulated by 051 accomplishes a key step in nutrient allocation between endosperm and embryo within maize seeds. Identification of this network will help uncover the mechanisms regulating the nutritional balance between endosperm and embryo.

关键词： 0S1 functions the allocation of nutrients between the endosperm and embryo in maize seeds^FA

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Maize WI5 encodes an endo-1,4-β-xylanase required for secondary cell wall synthesis and water transport in xylem

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Journal of Integrative Plant Biology 2020年第10期62卷 1607-1624页

作者： Xiaojiao Hu Yang Cui Xiaomin Lu Weibin Song Lei Lei Jinjie Zhu jinsheng Lai Lizhu E Haiming zhao State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China

Water transport from roots to leaves through xylem is important for plant growth and *** in water transport can cause drought stress,even when there is adequate water in the ***,we identi-fied the maize(Zea mays)wilty5(wi5)mutant,which ex-hibits marked dwarfing and leaf wilting throughout most of its life cycle under normal growth ***5 seed-lings exhibited lower xylem conductivity and wilted more rapidly under drought,NaCl,and high temperature treat-ments than wild-type ***-based cloning revealed that WI5 encodes an active endo-1,4-β-xylanase from gly-cosyl dehydration family 10,which mainly functions in degrading and reorganizing cell wall ***-transcription polymerase chain reaction andβ-glucuronidase assays revealed that WI5 is highly expressed in stems,especially in internodes undergoing secondary wall *** sequencing suggested that WI5 plays a unique role in internode *** and electron microscopy confirmed that wi5 is defective in xylan deposition and secondary cell wall *** deposition and xylan content were markedly re-duced in wi5 compared to the wild-type *** results suggest that WI5 functions in xylem cell wall thickening through its xylanase activity and thereby regulates xylem water transport,the drought stress response,and plant growth in maize.

关键词： dwarf fir transport

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Genesis of the Gold Deposit in the Indus-Yarlung Tsangpo Suture Zone,Southern Tibet:Evidence from Geological and Geochemical Data

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Acta Geologica Sinica(English Edition) 2017年第3期91卷 947-970页

作者： ZHANG Xiong DENG Xueguo YANG Zhusen HOU Zengqian ZHENG Yuanchuan LIU Yingchao zhao Xiaoyan XU Bo PEI Yingru ZHOU jinsheng zhao Miao YUAN Jianfei School of Earth sciences and Resources China University of GeosciencesBeijing MLR Key Laboratory of Metallogeny and Mineral Assessment Institute of Mineral ResourcesChinese Academy of Geological Sciences Institute of Metallurgical Geology and Exploration Sichuan Institute of Geology Chinese Academy of Geological Sciences ARC Centre of Excellence for Core to Crust Fluid System(CCFS) and GEMOC Department Of Earth and Planetary SciencesMacquarie University

The Nianzha gold deposit, located in the central section of the Indus-Yarlung Tsangpo suture （IYS） zone in southern Tibet, is a large gold deposit （Au reserves of 25 tons with average grade of 3.08 g/t） controlled by a E-W striking fault that developed during the main stage of Indo-Asian collision （-65-41 Ma）. The main orebody is 1760 m long and 5.15 m thick, and occurs in a fracture zone bordered by Cretaceous diorite in the hanging wall to the north and the Renbu tectonic melange in the footwall to the south. High-grade mineralization occurs in a fracture zone between diorite and ultramafic rock in the Renbu tectonic melange. The wall-rock alteration is characterized by silicification in the fracture zone, serpentinization and the formation of talc and magnesite in the uitramafic unit, and chloritization and the formation of epidote and calcite in diorite. Quartz veins associated with Au mineralization can be divided into three stages. Fluid inclusion data indicate that the deposit formed from H20-NaCl-organic gas fluids that homogenize at temperatures of 203℃-347℃ and have salinities of 0.35wt%-17.17wt% NaCI equivalent. The quartz veins yield δ18Ofluid values of 0.15‰-10.45‰, low δDv-SMow values （-173%o to -96%o）, and the δ13C values of-17.6‰ to -4.7‰, indicating the ore-forming fluids were a mix of metamorphic and sedimentary orogenic fluids with the addition of some meteoric and mantle-derived fluids. The pyrite within the diorite has δ34SV-CDT values of -2.9‰-1.9‰（average -1.1‰）, 206pb/204pb values of 18.47- 18.64, 207pb/204pb values of 15.64-15.74, and 208pb/204pb values of 38.71-39.27, all of which are indicative of the derivation of S and other ore-forming elements from deep in the mantle. The presence of the Nianzha, Bangbu, and Mayum gold deposits within the IYS zone indicates that this area is highly prospective for large orogenic gold deposits. We identified three types of mineralization within the IYS, namely Bangbu-type accretionary, Mayum-type micro

关键词： geology gold mineralization Nianzha Deposit Indus-Yarlung Tsangpo suture zone Tibet

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Effect of acupuncture treatment on smoking cessation in smokers from Hong Kong

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Journal of Traditional Chinese Medicine 2016年第5期36卷 634-639页

作者： Wang Yingying Liu zhao Zhang Ou Chen Min Huang Lingling Wu Yuan Zhang Fanfan Wu Guanyi He Xiuqing Yang jinsheng Institute of Acupuncture and Moxibustion China Academy of Chinese Medical Sciences Beijing 100700 China Tobacco Medicine Research and Clinical Smoking Cessation Center China-Japan Friendship Hospital Beijing 100029 China Acupuncture specialist centre of Hong Kong Pok Oi Hospital Hong Kong China

OBJECTIVE: To analyze the characteristics of smokers treated with acupuncture for smoking cessation in Hong ***: A total of 2051 subjects were recruited in a clinical pilot research project "acupuncture for smoking cessation", which was conducted jointly by Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences and Hong Kong Pok Oi Hospital from January of 2011 to December of 2013. The characteristics of study subjects, including baseline information, smoking background, intention to quit and influencing factors were ***: The majority of subjects treated with acupuncture for smoking cessation in Hong Kong was male(66.7%), but the proportion of female smokers in this study(33.3%) was higher than that of female smokers in Hong Kong population(13.8%, P <0.05). Subjects were at the mean age of 43.83 years old, of which the percentage of females aged 31-40 years was the highest(38.8%, P < 0.05). The mean duration of smoking was 25.49 years. The number of cigarettes smoked per day was 17.57 *** Test for Nicotine Dependence(FTND)was 5.29 points. Most of the subjects had attempted quitting smoking(81.42%). The confidence index(7.44 points) and the readiness to quit smoking(8.13 points) were high. Subjects quitting smoking were mostly due to health cause(81.91%). The majority of subjects were at the level of middle school(61.63%). The higher the educational level was, the lower the tobacco dependence was and the higher the confidence in successfully quitting smoking was. 50.27% of subjects chose acupuncture for smoking cessation mainly through friends, television and network publicity. Subjects who had received acupuncture had the highest confidence index, while those who wanted to try a new method had the lower confidence ***: Acupuncture for smoking cessation was more popular in female smokers, especially those aged 31-40 years. The effectiveness of acupuncture-smoking cessation was most sign

关键词： Acupuncture Smoking cessation Tobacco use disorder

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Improved atmospheric mercury simulation using updated gas-particle partition and organic aerosol concentrations

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Journal of Environmental Sciences 2022年第9期34卷 106-118页

作者： Kaiyun Liu Qingru Wu Shuxiao Wang Xing Chang Yi Tang Long Wang Tonghao Liu Lei Zhang Yu zhao Qin’geng Wang jinsheng Chen State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex Beijing 100084China Institute of Atmospheric Environment Guangdong provincial academy of environmental scienceGuangzhou 510045China China National Environmental Monitoring Centre Beijing 100012China State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing210023China Center for Excellence in Regional Atmos.Environ. Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China

The gaseous or particulate forms of divalent mercury(HgⅡ) significantly impact the spatial distribution of atmospheric mercury concentration and deposition flux(FLX). In the new nested-grid GEOS-Chem model, we try to modify the HgⅡ gas-particle partitioning relationship with synchronous and hourly observations at four sites in China. Observations of gaseous oxidized Hg(GOM), particulate-bound Hg(PBM), and PM 2.5 were used to derive an empirical gas-particle partitioning coefficient as a function of temperature( T) and organic aerosol(OA) concentrations under different relative humidity(RH). Results showed that with increasing RH, the dominant process of HgⅡ gas-particle partitioning changed from physical adsorption to chemical desorption. And the dominant factor of HgⅡ gas-particle partitioning changed from T to OA concentrations. We thus improved the simulated OA concentration field by introducing intermediate-volatility and semi-volatile organic compounds(I/SVOCs) emission inventory into the model framework and refining the volatile distributions of I/SVOCs according to new filed tests in the recent literatures. Finally, normalized mean biases(NMBs) of monthly gaseous element mercury(GEM), GOM, PBM, WFLX were reduced from-33%–29%, 95%–300%, 64%–261%, 117%–122% to-13%–0%,-20%–80%,-31%–50%,-17%–23%. The improved model explains 69%–98% of the observed atmospheric Hg decrease during 2013–2020 and can serve as a useful tool to evaluate the effectiveness of the Minamata Convention on Mercury.

关键词： Nested GEOS-Chem model HgⅡgas-particle partitioning Organic aerosol Atmospheric mercury Mercury deposition flux

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新工科信息类专业开放式实践教学平台研究

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廊坊师范学院学报（自然科学版） 2022年第1期22卷 99-103页

作者：任建强张玲娟冯越张春红王顺晔赵金生朱得华廊坊师范学院河北廊坊065000 廊坊师范学院学科建设办公室河北廊坊065000 廊坊师范学院电子信息工程学院河北廊坊065000

高校新工科建设对培养创新型卓越人才意义重大。实践教学质量的提升是新工科人才培养的关键,但当前很多院校普遍存在教学模式单调、实验时间受限等现实问题。对此,以信息类专业这一新工科重点建设专业群为例,基于微信应用号和嵌入式系... 详细信息

高校新工科建设对培养创新型卓越人才意义重大。实践教学质量的提升是新工科人才培养的关键,但当前很多院校普遍存在教学模式单调、实验时间受限等现实问题。对此,以信息类专业这一新工科重点建设专业群为例,基于微信应用号和嵌入式系统开发技术设计实现了一种开放式实践教学平台,进行了教学视频、文档资源、开放实验管理、实践报告及辅助功能等模块的建设。试用测试结果表明,平台为学生移动学习、实验预约及开放设备自动管理提供了良好支持,具有使用更方便、功能更完备的特点,能够使学生随时、随地、随需地全时空开放式学习、实践及互动。

关键词：高校新工科开放式实践教学

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