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Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice

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Journal of Integrative Plant Biology 2023年第4期65卷 918-933页

作者： Yingxiu li Shichen Han Xingming Sun Najeeb Ullah Khan Qun Zhong Zhanying Zhang Hongliang Zhang Feng Ming Zichao li Jinjie Li State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Sanya Institute of China Agricultural University Sanya 572025China

Drought is a major factor restricting the production of rice(Oryza sativa L.).The identification of natural variants for drought stress-related genes is an important step toward developing genetically improved rice varieties.Here,we characterized a member of the SQUAMOSA PROMOTER BINDING PROTEIN-liKE(SPL)family,OsSPL10,as a transcription factor involved in the regulation of drought tolerance in rice.OsSPL10 appears to play a vital role in drought tolerance by controlling reactive oxygen species(ROS)production and stomatal movements.Haplotype and allele frequency analyses of OsSPL10 indicated that most upland rice and improved lowland rice varieties harbor the OsSPL10Hap1allele,whereas the OsSPL10Hap2allele was mainly present in lowland and landrace rice varieties.Importantly,we demonstrated that the varieties with the OsSPL10Hap1allele showed low expression levels of OsSPL10 and its downstream gene,OsNAC2,which decreases the expression of OsAP37 and increases the expression of OsCOX11,thus preventing ROS accumulation and programmed cell death(PCD).Furthermore,the knockdown or knockout of OsSPL10 induced fast stomatal closure and prevented water loss,thereby improving drought tolerance in rice.Based on these observations,we propose that OsSPL10 confers drought tolerance by regulating OsNAC2 expression and that OsSPL10Hap1could be a valuable haplotype for the genetic improvement of drought tolerance in rice.

关键词： drought tolerance haplotype OsNAC2 OsSPL10 ROS

Time-frequency Feature Extraction Method of the Multi-Source Shock Signal Based on Improved VMD and Bilateral Adaptive Laplace Wavelet

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Chinese Journal of Mechanical Engineering 2023年第2期36卷 166-179页

作者： Nanyang Zhao jinjie Zhang Zhiwei Mao Zhinong Jiang He li Beijing Key Laboratory of High-end Mechanical Equipment Health Monitoring and Self-Recovery Beijing University of Chemical TechnologyBeijing 100029China

Vibration signals have the characteristics of multi-source strong shock coupling and strong noise interference owing to the complex structure of reciprocating machinery.Therefore,it is difficult to extract,analyze,and diagnose mechanical fault features.To accurately extract sensitive features from the strong noise interference and unsteady monitoring signals of reciprocating machinery,a study on the time-frequency feature extraction method of multi-source shock signals is conducted.Combining the characteristics of reciprocating mechanical vibration signals,a targeted optimization method considering the variational modal decomposition(VMD)mode number and second penalty factor is proposed,which completed the adaptive decomposition of coupled signals.Aiming at the bilateral asymmetric attenuation characteristics of reciprocating mechanical shock signals,a new bilateral adaptive Laplace wavelet(BALW)is established.A search strategy for wavelet local parameters of multi-shock signals is proposed using the harmony search(HS)method.A multi-source shock simulation signal is established,and actual data on the valve fault are obtained through diesel engine fault experiments.The fault recognition rate of the intake and exhaust valve clearance is above 90%and the extraction accuracy of the shock start position is improved by 10°.

关键词： Shock Signal processing Wavelet VMD Fault diagnosis Diesel engine

Synthesis of cycloparaphenylene under spatial nanoconfinement

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Chinese Chemical Letters 2023年第6期34卷 298-300页

作者： Jinjie Li Huixian Jin Zhikun Shang Jie Wang Donglai Tian Yun Ding Aiguo Hu Shanghai Key Laboratory of Advanced Polymeric Materials School of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China

Suzuki coupling reactions between symmetrical monomers were conducted in various mesoporous silica nanoreactors grafted with palladium catalysts,enabling the selective formation of[12]cycloparaphenylene precursor with separate yield up to 25%in one-pot reactions,much higher than that in homogeneous reaction.The spatial nanoconfinement of the nanoreactors promotes the macrocyclization while limits the concomitant linear oligomer formation,offering more possibilities for the synthesis of macrocycles from symmetrical monomers in one-pot reaction.

关键词： Nanoreactor Spatial confinement Cyclization reaction One-pot reaction Suzuki coupling reaction

Micro-Sized Pinhole Inspection with Segmented Time Reversal and High-Order Modes Cluster Lamb Waves Based on EMATs

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Chinese Journal of Mechanical Engineering 2023年第1期36卷 224-236页

作者： jinjie Zhou Yang Hu Xiang li Yang Zheng Sanhu Yang Yao liu School of Mechanical Engineering North University of ChinaTaiyuan030051China School of Aeronautics and Astronautics University of Electronic Science and Technology of ChinaChengdu611731China China Special Equipment Inspection and Research Institute Beijing100029China

Pinhole corrosion is difficult to discover through conventional ultrasonic guided waves inspection,particularly for micro-sized pinholes less than 1 mm in diameter.This study proposes a new micro-sized pinhole inspection method based on segmented time reversal(STR)and high-order modes cluster(HOMC)Lamb waves.First,the principle of defect echo enhancement using STR is introduced.Conventional and STR inspection experiments were conducted on aluminum plates with a thickness of 3 mm and defects with different diameters and depths.The parameters of the segment window are discussed in detail.The results indicate that the proposed method had an amplitude four times larger than of conventional ultrasonic guided waves inspection method for pinhole defect detection and could detect micro-sized pinhole defects as small as 0.5 mm in diameter and 0.5 mm in depth.Moreover,the segment window location and width(5-10 times width of the conventional excitation signal)did not affect the detection sensitivity.The combination of low-power and STR is more conducive to detection in different environments,indicating the robustness of the proposed method.Compared with conventional ultrasonic guided wave inspection methods,the proposed method can detect much smaller defect echoes usually obscured by noise that are difficult to detect with a lower excitation power and thus this study would be a good reference for pinhole defect detection.

关键词： Pinhole corrosion High-order modes cluster Lamb waves Segmented time reversal inspection Electromagnetic acoustic transducer

On the electric behaviour of conductive grease inside the contact zone

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Friction 2024年第4期12卷 683-697页

作者： Yuxin ZHOU Jinjie LiU Xinming li He CHONG Bo HAN Qinghua BAI SKF(Shanghai)Automotive Technologies Co. Ltd.Shanghai 201814China School of Mechanical and Automotive Engineering Qingdao University of TechnologyQingdao 266250China

Conductive grease has been considered as a potential solution in addressing electric erosion issue inside motor bearings.Understanding the conductive grease performance,especially the electric and tribological behaviour is crucial for its application.This paper combines bulk grease characterization and contact film study considering both electrical and tribological loading conditions.The results show that the electric performance for the selected conductive grease sample is electric field dependent,including both frequency and voltage amplitude.Its film impedance inside the contact area is closely related to the external electric field,running condition,and lubrication regime.The following film impedance calculation tells that not only bulk grease electric parameters,but the contact parameter,lubricant film contour and lubrication condition should be considered when performing the overall bearing electrical modelling.

关键词： Electric erosion lubricant conductivity grease film impedance

A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13

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Science China(life Sciences) 2022年第7期65卷 1456-1465页

作者： lina li Canxing Duan Jianfeng Weng Xiantao Qi Changlin liu Xinhai li jinjie Zhu Chuanxiao Xie National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural Sciences(CAAS)Beijing 100081 China Biotechnology Research Institute Chinese Academy of Agricultural Sciences(CAAS)Beijing 100081China

For some Cas nucleases,trans-cleavage activity triggered by CRISPR/Cas-mediated cis-cleavage upon target nucleic acid recognition has been explored for diagnostic detection.Portable single and multiplex nucleic acid-based detection is needed for crop pathogen management in agriculture.Here,we harnessed and characterized RfxCas13d as an additional CRISPR/Cas nucleic acid detection tool.We systematically characterized AsCas12a,LbCas12a,LwaCas13a,and RfxCas13d combined with isothermal amplification to develop a CRISPR/Cas nucleic acid-based tool for single or multiplex pathogen detection.Our data indicated that sufficient detection sensitivity was achieved with just a few copies of DNA/RNA targets as input.Using this tool,we successfully detected DNA from Fusarium graminearum and Fusarium verticillioides and RNA from rice black-streaked dwarf virus in crude extracts prepared in the field.Our method,from sample preparation to result readout,could be rapidly and easily deployed in the field.This system could be extended to other crop pathogens,including those that currently lack a detection method and have metabolite profiles that make detection challenging.This nucleic acid detection system could also be used for single-nucleotide polymorphism genotyping,transgene detection,and qualitative detection of gene expression in the field.

关键词： nucleic acid detection AsCas12a LbCas12a LwaCas13a RfxCas13d maize ear rot Fusarium head blight rice black-streaked dwarf virus(RBSDV)

Polyphenols Extracted from Enteromorpha clathrata Induce Apoptosis in Hepa1-6 Cell by Activating the Mitochondrial Apoptosis Signaling Pathways

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中国海洋大学学报:英文版 2023年第5期22卷 1393-1402页

作者： ZHANG Panxue SUN Bolun TONG Jingjing XIA Geran ZHANG jinjie li Chao YANG Wenge College of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315211China Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province Ningbo UniversityNingbo 315211China

Green alga Enteromorpha clathrata(E.clathrata)contains a variety of bioactive compounds,including polysaccharides,polyphenols and fat-soluble pigments etc.,among which polyphenols exhibit a wide range of medicinal properties.E.clathrata polyphenols(ECPs)have shown various biological activities such as antioxidant,anti-inflammatory and antidiabetic effects;however,the potential of ECPs as an anti-cancer reagent remains unclear.The aim of this study was to investigate the anti-tumor activity and underlying mechanisms of ECPs on hepatocellular carcinoma.The cytotoxicity of Hepa1-6 cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and lactate dehydrogenase(LDH)assay.Flow cytometry and fluorescence microscope analysis of cell apoptosis after annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI)staining.2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA)assay was used for intracellular reactive oxygen species(ROS)detection.caspase-9 activity was determined using cspase-9 colorimetric assay.Mitochondrial transmembrane potential(Δψm)was measured using JC-1.Western blot and quantitative real-time PCR(qPCR)were used to assess the expressions of the apoptosis regulators Bax,Bcl-2,cytochrome c and caspase-3.It was found that ECPs showed a dose-dependent cytotoxicity against Hepa1-6 cells by inducing apoptosis.The apoptosis in ECPs-treated Hepa1-6 cells was accompanied by the loss of mitochondrial membrane potential,elevated ROS generation,increased release of mitochondrial cytochrome c,and up-regulation of caspase-9 and caspase-3.The expressions of Bax(pro-apoptotic molecule)and Bcl-2(apoptosis suppressor)were up-regulated and down-regulated,respectively,at both mRNA and protein levels.These molecular alterations revealed that ECPs caused apoptosis of cells through the mitochondrial pathway,suggesting that ECPs are potential candidates to be developed for liver cancer treatment.

关键词： Enteromorpha clathrate polyphenol anti-cancer activity apoptosis mitochondrial-dependent pathway

Whisker of biphasic calcium phosphate ceramics: Osteoimmunomodulatory behaviors

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Nano Research 2022年第10期15卷 9169-9182页

作者： jinjie Wu Cong Feng Menglu Wang Hongfeng Wu Xiangdong Zhu Xiangfeng li Xuening Chen Xingdong Zhang National Engineering Research Center for Biomaterials College of Biomedical EngineeringSichuan UniversityChengdu 610064China

Immune systems play a critical role in the regulation of bone formation and homeostasis,which arouses a growing interest in the development of biomaterials that can modulate both immune response and osteogenesis.In this study,biphasic calcium phosphate(BCP)ceramics were modified with different whiskered surface,and their effects on macrophage polarization and functional status were investigated.The results showed that compared to BCP-W ceramics with long and solid whiskers,BCPHW ceramics with short and hollow whiskers surface were conducive to protein adsorption and macrophage elongation.Furthermore,BCP-HW ceramics down-regulated the expression of M1 macrophage markers(Il1β,Tnfα,and iNos),promoted the expression of M2 macrophage markers(Il10 and Arg)and growth factors(Tgfβ1 and Bmp2),which might be attributed to the differential integrin expression regulated by different whisker structures.The conditioned medium derived from the supernatant of macrophage/whiskered ceramic co-culture was further used to culture MC3T3-E1 pre-osteoblasts to evaluate the effects of whiskered ceramic-mediated macrophage secretion on osteogenesis in vitro.Compared with BCP-W ones,the secretion pattern induced by BCP-HW ceramics could promote the expression of bone markers in pre-osteoblasts,which might due to the activation of intracellular signaling cascades like BMP/Smad and TGF-β/Smad signaling pathways.A murine intramuscular implantation model suggested that after implantation for 1,2,and 3 weeks,BCP-HW ceramics drove the switch of macrophages to ARG+wound-healing M2 phenotype,while BCP-W ceramics increased the proportion of iNOS+M1 inflammatory macrophages.At 2 months,only BCP-HW could induce ectopic bone formation.Taken together,these results indicated that BCP ceramics with hollow whiskers were capable of creating a proper inflammatory microenvironment to induce bone formation.These whiskered BCP ceramics with good osteo-immunomodulatory capacity hold promise in serving as bone grafts to achieve desired bone repair and regenerat

关键词： whisker macrophage polariztion osteoinduction biphasic calcium phosphate surface structure

Constructing Nanoscale Topology on the Surface of Microfibers Inhibits Fibroblast Fibrosis

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Advanced Fiber Materials 2022年第5期4卷 1219-1232页

作者： Yongjie Jiao Xiaojing li jinjie Chen Chaojing li Laijun liu Xingxing liu Fujun Wang Guang Chen Lu Wang Key Laboratory of Textile Science and Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai 201620China Healthcare Department of Taizhou Hospital Affiliated to Wenzhou Medical University(Zhejiang Province Taizhou Hospital) Taizhou 317000China Department of Vascular Surgery Taizhou Hospital Affiliated to Wenzhou Medical University(Zhejiang Province Taizhou Hospital)Taizhou 317000China

Fibrosis is a common problem in soft tissue regeneration,often caused by the differentiation of fibroblasts into myofibro-blasts.Because of the nanoscale topology that regulates the mechanical transduction of cells,nanofibers or nanoparticles are commonly used to modulate fibroblast differentiation.However,the strength of nanofibers is insufficient,and the physi-ological toxicity of nanoparticles still remains to be verified.In this study,self-induced crystallization was used to construct nano-protrusions on the random and aligned polycaprolactone microfibers to regulate the behavior of fibroblasts.The results revealed that the mechanical properties of microfibers with a nanoscale topology were improved.Immunofluorescence staining manifested that nano-protrusions impeded the activation of integrins and vinculins,thereby inhibiting the nuclear transfer of Yes-associated protein,resulting in a decrease in the expression ofα-smooth muscle actin.Nanoscale topology of microfibers hampered the activation of the Rho/ROCK signalling pathway.In general,we used a simple process to con-struct a fibrous scaffold with a micro-nano multilevel structure.This structure can hinder the transformation of fibroblasts into myofibroblasts on both random and aligned fibers,which is expected to prevent fibrosis.

关键词： Electrospinning Topology Fibroblast Fibrosis