Constructing Nanoscale Topology on the Surface of Microfibers Inhibits Fibroblast Fibrosis

作     者：Yongjie Jiao Xiaojing Li Jinjie Chen Chaojing Li Laijun Liu Xingxing Liu Fujun Wang Guang Chen Lu Wang 

作者机构：Key Laboratory of Textile Science and TechnologyMinistry of EducationCollege of TextilesDonghua UniversityShanghai 201620China Healthcare Department of Taizhou Hospital Affiliated to Wenzhou Medical University(Zhejiang Province Taizhou Hospital)Taizhou 317000China Department of Vascular SurgeryTaizhou Hospital Affiliated to Wenzhou Medical University(Zhejiang Province Taizhou Hospital)Taizhou 317000China 

出 版 物：《Advanced Fiber Materials》 (先进纤维材料（英文）)

年 卷 期：2022年第4卷第5期

页      面：1219-1232页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0701[理学-数学] 

基　　金：Supported by Shanghai Sailing Program(Grant No.20YF1400800) Shanghai Science and Technology Development Fund(21S31900700) 111 Project 2.0(Grant no.BP0719035). 

主　　题：Electrospinning Topology Fibroblast Fibrosis 

摘      要：Fibrosis is a common problem in soft tissue regeneration,often caused by the differentiation of fibroblasts into myofibro-blasts.Because of the nanoscale topology that regulates the mechanical transduction of cells,nanofibers or nanoparticles are commonly used to modulate fibroblast differentiation.However,the strength of nanofibers is insufficient,and the physi-ological toxicity of nanoparticles still remains to be verified.In this study,self-induced crystallization was used to construct nano-protrusions on the random and aligned polycaprolactone microfibers to regulate the behavior of fibroblasts.The results revealed that the mechanical properties of microfibers with a nanoscale topology were improved.Immunofluorescence staining manifested that nano-protrusions impeded the activation of integrins and vinculins,thereby inhibiting the nuclear transfer of Yes-associated protein,resulting in a decrease in the expression ofα-smooth muscle actin.Nanoscale topology of microfibers hampered the activation of the Rho/ROCK signalling pathway.In general,we used a simple process to con-struct a fibrous scaffold with a micro-nano multilevel structure.This structure can hinder the transformation of fibroblasts into myofibroblasts on both random and aligned fibers,which is expected to prevent fibrosis.