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Alterations of the Ca2＋ signaling pathway in pancreatic beta-cells isolated from db/db mice

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Protein & Cell 2014年第10期5卷 783-794页

作者： Kuo Liang Wen Du jingze lu Fei Li lu Yang Yanhong Xue Bertil Hille Liangyi Chen Department of General Surgery XuanWu Hospital Capital Medical University Beijing 100053 China National Key Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Science Beijing 100101 China The State Key Laboratory of Biomembrane and Membrane Biotechnology Beijing Key Laboratory of CardiometabolicMolecular Medicine Institute of MolecuLar Medicine Peking University Beijing 100871 China Department of Physiology and Biophysics University of Washington School of Medicine Seattle WA 98195 USA

Upon glucose elevation, pancreatic beta-cells secrete insulin in a Ca2＋-dependent manner. In diabetic animal models, different aspects of the calcium signaling path- way in beta-cells are altered, but there is no consensus regarding their relative contributions to the development of beta-cell dysfunction. In this study, we compared the increase in cytosolic Ca2＊（[Ca2＊]~） via Ca2＋ influx, Ca2＊ mobilization from endoplasmic reticulum （ER） calcium stores, and the removal of Ca2＋ via multiple mechanisms in beta-cells from both diabetic db/db mice and non- diabetic C57BL/6J mice. We refined our previous quan- titative model to describe the slow [Ca2＋]i recovery after depolarization in beta-cells from db/db mice. According to the model, the activity levels of the two subtypes of the sarco-endoplasmic reticulum Ca2＋-ATPase （SERCA） pump, SERCA2 and SERCA3, were severely down-reg- ulated in diabetic cells to 65% and 0% of the levels in normal cells. This down-regulation may lead to a reduc- tion in the Ca2＋ concentration in the ER, a compensatory up-regulation of the plasma membrane Na＋/Ca2＋ exchanger （NCX） and a reduction in depolarizationevoked Ca2＋ influx. As a result, the patterns of glucosestimulated calcium oscillations were significantly different in db/db diabetic beta-cells compared with normal cells. Overall, quantifying the changes in the calcium signaling pathway in db/db diabetic beta-cells will aid in the development of a disease model that could provide insight into the adaptive transformations of beta-cell function during diabetes development.

关键词： diabetic beta-cells calcium signalingalterations SERCA pump db/db mice

Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells

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Protein & Cell 2012年第8期3卷 618-626页

作者： Du Wen Yanhong Xue Kuo Liang Tianyi Yuan jingze lu Wei Zhao Tao Xu Liangyi Chen National Key Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Lab of Cell Secretion and Metabolism Institute of Molecular MedicinePeking University and National Center for Nanoscience and TechnologyBeijing100871China Department of General Surgery Xuanwu HospitalCapital Medical UniversityBeijing 100053China Graduate School of the Chinese Academy of Sciences Beijing 100864China

Although bulk endocytosis has been found in a number of neuronal and endocrine cells,the molecular mechanism and physiological function of bulk endocytosis remain *** pancreatic beta cells,we have observed bulk-like endocytosis evoked both by flash photolysis and trains of ***-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca^(2+) entry and suppressed by the inhibition of dynamin ***,defects in bulklike endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice,which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.

关键词： bulk-like endocytosis clathrin-independent endocytosis dynamin diabetic KKAy mice

HID-1 is a peripheral membrane protein primarily associated with the medial-and transGolgi apparatus

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Protein & Cell 2011年第1期2卷 74-85页

作者： Lifen Wang Yi Zhan Eli Song Yong Yu Yaming Jiu Wen Du jingze lu Pingsheng Liu Pingyong Xu Tao Xu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate School of the Chinese Academy of Sciences Beijing 100864China College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China

Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation(Hid)*** the fact that the hid-1 gene encodes a novel protein(HID-1)which is highly conserved from Caenorhabditis elegans to mammals,the domain structure,subcellular localization,and exact function of HID-1 remain *** studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional *** this study,we revealed that mammalian HID-1 localized to the medial-and transGolgi apparatus as well as the cytosol,and the localization was sensitive to brefeldin A ***,we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the ***,we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi *** propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.

关键词： HID-1 Golgi peripheral membrane protein fluorescent recovery after photobleaching N-myristoylation

hTAC internalizes via both clathrin-dependent and clathrin-independent endocytosis in mammalian cells

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Protein & Cell 2018年第10期9卷 896-901页

作者： Xinyu Zhu Min Li Xiaojun Xu Rui Zhang Xiaofei Zhang Zhuo Ma jingze lu Tao Xu Junjie Hou Eli Song Key Laboratory of Molecular Biophysics of the Ministry of Educa-tion College of Life Science and Technology Huazhong Universityof Science and Technology Wuhan 430074 China National Laboratory of Biomacromolecules CAS Center forExcellence in Biomacromolecules Institute of Biophysics ChineseAcademy of Sciences Beijing 100101 China College of Life Sciences University of Chinese Academy ofSciences Beijing 100049 China

Dear Editor, Endocytosis is a crucial process employed by cells to internal- ize nutrients and turnover membrane components and is essential for many functions, including nutrient uptake, signal transduction, cytokinesis, morphogenesis, cell adhesion and migration. Endocytosis is classified as clathrin-dependent endocytosis （CDE） or clathrin-independent endocytosis （CIE） according to its dependence on clathrin. Several different CIE pathways have been proposed, including caveolin-dependent endocytosis, flotillin-dependent endocytosis, the clathrin-inde- pendent carrier pathway, ARF6-dependent endocytosis, phagocytosis, macropinocytosis, the IL2RI3 pathway （Doherty and McMahon, 2009）, the newly identified fast endophilin-me- diated endocytosis pathway （Boucrot et al., 2015） and the EGFR-NCE pathway （Caldieri et al., 2017）.

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基于膨胀栅格的多目标站点AGV路径规划方法

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机械设计与研究 2023年第6期39卷 6-10,21页

作者：吕竞则乔文俊刘顺西南大学计算机与信息科学学院软件学院重庆400715 上海交通大学机械与动力工程学院上海200240 上海交大智邦科技有限公司上海201306

针对离散制造、环境多变的脉动装配线特点,以及常规栅格建模环境下自动导引车(Automated Guided Vehicle,ACV)单一目标运动的局限性,提出一种膨胀栅格建模的改进方法,并结合蚁群算法进行优化与应用。首先在常规栅格基础上增设膨胀栅格... 详细信息

针对离散制造、环境多变的脉动装配线特点,以及常规栅格建模环境下自动导引车(Automated Guided Vehicle,ACV)单一目标运动的局限性,提出一种膨胀栅格建模的改进方法,并结合蚁群算法进行优化与应用。首先在常规栅格基础上增设膨胀栅格进行建模,以此适应环境多变的脉动生产线特点;然后,在传统路径规划的蚁群算法基础上,引入基于精英策略的信息素更新方法与最大最小蚂蚁系统,提出改进的多任务轨迹规划算法。经仿真实验验证,优化蚁群算法在整体路径长度与收敛性上优于传统算法和其他路径规划算法。仿真实验还分别模拟了分段路径与不同占比障碍栅格单元的优化修正效果:当分成若干段路径时,中转站越靠近障碍栅格,优化算法影响越大;当障碍栅格占比不同时,在路径可控范围内的障碍栅格占比越高,优化算法的效果越理想。

关键词：自动导引车(AGV) 路径规划蚁群算法膨胀栅格

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低油价下投资加拿大油气面临的挑战和机会

国际石油经济 2015年第10期23卷 6-10页

作者：王阳陆京泽国家开发银行

介绍低油价对加拿大石油公司的影响,分析加拿大石油公司在低油价环境下的投资策略,并对未来在低油价环境下的投资策略进行探讨,认为目前加拿大油气市场值得一些具有长期投资目标的资金(包括中国投资者)予以关注。建议投资者一是寻求能... 详细信息

介绍低油价对加拿大石油公司的影响,分析加拿大石油公司在低油价环境下的投资策略,并对未来在低油价环境下的投资策略进行探讨,认为目前加拿大油气市场值得一些具有长期投资目标的资金(包括中国投资者)予以关注。建议投资者一是寻求能够产出现金流的项目,利用市场的低谷来找到投资机会,等待油价回升;二是寻求长期资金并以股权方式为主进行融资,降低财务风险;同时要有一个优秀的加拿大本地管理团队,以便充分利用加拿大的公司运作、企业文化、石油行业的规则以及本地资源等。

关键词：加拿大低油价油气行业石油公司油砂项目投资机会

Effects of urea-N and CO_(2) coupling fertilization on the growth,photosynthesis,yield and anthocyanin content of hydroponic purple cabbage Brassica campestris ***

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International Journal of Agricultural and Biological Engineering 2023年第5期16卷 123-131页

作者： Danyan Chen Yuanyuan Feng Ya Liu Juan Hu Shilong Li jingze Ma Xinyu lu Xiaorui Jiang Shuhui Sun Zhi Yang Changyi Wang College of Horticulture Jinling Institute of TechnologyNanjing 210038China Co-Innovation Center for Sustainable Forestry in Southern China College of ForestryNanjing Forestry UniversityNanjing 210037China School of Software Engineering Jinling Institute of TechnologyNanjing 211169China Powerchina Zhongnan Engineering Co. LtdChangsha 410014China College of Agricultural Science and Engineering Hohai UniversityNanjing 210098China China Coal Technology Engineering Group Nanjing Design and Research Institute Co.Ltd Nanjing 210014China

Water and fertilizer coupling is a high-efficiency technology for the development of facility ***,the interaction effect of nitrogen(N)and air carbon dioxide(CO_(2))on hydroponic purple cabbage,especially on its leaf anthocyanins under hydroponic solution systems,remains *** this study,six treatments were set as C0N0,C0N2,C0N4,C1N0,C1N2 and C1N4,with N0,N2 and N4 being 0.0 g/L,0.2 g/L and 0.4 g/L exogenous urea-N to hydroponic solution dilution,respectively.C0 and C1 were set as with and without CO_(2)fertilizer(i.e.,800 g CO_(2)agent added one week after transplanting and 600 g CO_(2)agent added when the plant reached 15 cm in height),*** experiments were conducted to investigate the interaction effect of N and air CO_(2)(N×CO_(2))on the growth,photosynthesis,yield and anthocyanin content of hydroponic purple cabbage Brassica campestris *** results showed N×CO_(2)extremely significantly influenced plant height(H),net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular oxidation concentration(Ci),transpiration rate(Tr),leaf water use efficiency(LWUE)and *** C1N0 treatment had the largest yield at 262.5 g/plant,with higher values for root length,root weight,plant height and leaf number than the other *** Pn,Ci and Tr of C1N4 were the highest at 3.05μmol CO_(2)/m2·s,352.8μmol CO_(2)/m2·s and 2.31 mmol H2O/m2·s,*** C1N2 treatment received the largest Gs value of 0.70 mol H2O/m2·s and the largest Tr of 2.31 mmol H2O/m2·*** was the highest LWUE for C0N2(1.41)and the highest anthocyanin content for C1N2(1.35 mg/kg).There was a significant negative correlation between leaf number and anthocyanin(r=-0.414,plution dilution is a potential N×CO_(2)coupling strategy to increase anthocyanin and the yield of purple cabbage.

关键词： nitrogen concentration carbon dioxide purple cabbage hydroponic solution anthocyanins

A genome-wide association study of facial morphology identifies novel genetic loci in Han Chinese

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Journal of Genetics and Genomics 2021年第3期48卷 198-207页

作者： Yin Huang Dan Li lu Qiao Yu Liu Qianqian Peng Sijie Wu Manfei Zhang Yajun Yang jingze Tan Shuhua Xu Li Jin Sijia Wang Kun Tang Stefan Grunewald Chinese Academy of Sciences Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesCASShanghai 200031China DeepBlue Technology(Shanghai)Co. LtdShanghai 200336China State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology School of Life SciencesFudan UniversityShanghai 200433China Fudan-Taizhou Institute of Health Sciences Taizhou 225300China Collaborative Innovation Center of Genetics and Development Shanghai 200438China f Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

The human face is a heritable surface with many complex sensory *** recent years,many genetic loci associated with facial features have been reported in different populations,yet there is a lack of studies on the Han Chinese ***,we report a genome-wide association study of 3D normal human faces of 2,659 Han Chinese with autosegment phenotypes of facial *** identify singlenucleotide polymorphisms(SNPs)encompassing four genomic regions showing significant associations with different facial regions,including SNPs in DENND1 B associated with the chin,SNPs among PISRT1 associated with eyes,SNPs between DCHS2 and SFRP2 associated with the nose,and SNPs in VPS13 B associated with the *** replicate 24 SNPs from previously reported genetic loci in different populations,whose candidate genes are DCHS2,SUPT3 H,HOXD1,SOX9,PAX3,and *** results provide a more comprehensive understanding of the genetic basis of variation in human facial morphology.

关键词： Genome-wide association study Facial morphology Automatic phenotyping Visualization

二酰基甘油指引网格蛋白包裹小窝沿微管运动到胞吐位置介导胞吞胞吐的耦联

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科学新闻 2016年第1期 103-103页

作者： Tianyi Yuan Lin Liu Yongdeng Zhang Lisi Wei Shiqun Zhao Xiaolu Zheng Xiaoshuai Huang Jerome Boulanger Charles Gueudry jingze lu Lihan Xie Wen Du Weijian Zong lu Yang Jean Salamero 刘彦梅陈良怡北京大学分子医学研究所

文章简介细胞生物学教科书上认为,受体介导的胞吞由组成性的网格蛋白包裹小窝所介导,后者在细胞膜随机的位置出芽并缓慢地从细胞膜上脱离(60～100秒)。与之观点相反,在可兴奋细胞中,与调节型胞吐耦联发生的网格蛋白介导的胞吞发生在胞... 详细信息

文章简介细胞生物学教科书上认为,受体介导的胞吞由组成性的网格蛋白包裹小窝所介导,后者在细胞膜随机的位置出芽并缓慢地从细胞膜上脱离(60～100秒)。与之观点相反,在可兴奋细胞中,与调节型胞吐耦联发生的网格蛋白介导的胞吞发生在胞吐位置附近,且在胞吐后迅速发生(10秒)。调节型胞吞与胞吐时空耦联的背后机制仍不清楚。本研究发现与胞吐耦联的胞吞利用预先形成的网格蛋白包裹小窝,在囊泡分泌后迅速运动到胞吐位置,然后被

关键词：胞吐胞吞出胞网格蛋白小窝微管介导包裹耦联