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Novel technologies in cfDNA analysis and potential utility in clinic

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Chinese Journal of Cancer Research 2021年第6期33卷 708-718页

作者： Jie Li Mengyue Xu Junya Peng jingqiao wang Yupei Zhao Wenming Wu Xun Lan Department of Basic Medical Sciences School of MedicineTsinghua UniversityBeijing 100084China Department of General Surgery Peking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegeBeijing 100730China Department of Medical Research Peking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegeBeijing 100730China Tsinghua-Peking Joint Center for Life Sciences Tsinghua UniversityBeijing 100084China Department of State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100730China

The profiling of plasma cell-free DNA(cfDNA)is becoming a valuable tool rapidly for tumor diagnosis,monitoring and *** plasma cfDNA technologies have been in routine or emerging use,including analyses of mutations,copy number alterations,gene fusions and DNA ***,new technologies in cfDNA analysis have been developed in laboratories,and potentially reflect the status of epigenetic modification,the immune microenvironment and the microbiome in tumor *** this review,the authors discuss the principles,methods and effects of the current cfDNA assays and provide an overview of studies that may inform clinical applications in the near future.

关键词： cfDNA liquid biopsy cancer diagnosis recurrence monitoring therapy response

Newly Identified Essential Amino Acids Affecting Chlorella ellipsoidea DGAT1 Function Revealed by Site-Directed Mutagenesis

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Newly Identified Essential Amino Acids Affecting Chlorella e...

Curve interpolation based on Catmull-Clark subdivision scheme

中国生物工程学会第十三届学术年会暨2019年全国生物技术大会

作者： Baocheng Sun Xuejie Guo Chengming Fan Yuhong Chen jingqiao wang Zanmin Hu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences Institute of Economical Crops Yunnan Agricultural Academy

Diacylglycerol acyltransferase(DGAT) is a rate-limiting enzyme in the synthesis of triacylglycerol(TAG), the most important form of energy storage in plants. Some residues have previously been proven to be crucial for DGAT1 activity. In this study, we used site-directed mutagenesis of the CeDGAT1 gene from Chlorella ellipsoidea to alter 16 amino acids to investigate effects on DGAT1 function. Of the 16 residues(L482 R, E542 R, Y553 A, G577 R, R579 D, Y582 R, R596 D,H603 D, H609 D, A624 R, F629 R, S632 A, W650 R, A651 R, Q658 H, and P660 R), we newly identified 5(L482, R579, H603, A651, and P660) as being essential for DGAT1 function and 7(E542, G577, R596,H609, A624, S632, and Q658) that significantly affect DGAT1 function to different degrees, as revealed by heterologous expression of the mutants in yeast strain INVSc1. Importantly, compared with Ce DGAT1, expression of the mutant CeDGAT1 Y553 A significantly increased the total fatty acid and TAG contents of INVSc1. Comparison among CeDGAT1 Y553 A, Gm DGAT1 Y341 A, At DGAT1 Y364 A,BnDGAT1 Y347 A, and Bo DGAT1 Y352 A, in which tyrosine at the position corresponding to the 553 rd residue in CeDGAT1 is changed into alanine, indicated that the impact of changing Y to A at position 553 is specific for CeDGAT1. Overall, the results provide novel insight into the structure and function of DGAT1, as well as a mutant gene with high potential for lipid improvement in microalgae and plants.

关键词： diacylglycerol acyltransferase site-directed mutagenesis heterologous expression total fatty acid content triacylglycerol

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Progress in Natural Science:Materials International 2003年第2期 64-70页

作者： ZHANG jingqiao and wang Guojin(State Key Laboratory of CAD&CG, Institute of Computer Images and Graphics, Zhejiang University, Hangzhou 310027, China)

Abstract An efficient algorithm for curve interpolation is proposed. The algorithm can produce a subdivision surface that can in-terpolate the predefined cubic B-spline curves by applying the Catmull-Clark scheme to a polygonal mesh containing "symmetric zonalmeshes", which possesses some special properties. Many kinds of curve interpolation problems can be dealt with by this algorithm, such asinterpolating single open curve or closed curve, a mesh of nonintersecting or intersecting curve. The interpolating surface is C~2 everywhereexcepting at a finite number of points. At the same time, sharp creases can also be modeled on the limit subdivision surface by duplicatingthe vertices of the tagged edges of initial mesh, i. e. the surface is only C~0 along the cubic B-spline curve that is defined by the taggededges. Because of being simple and easy to implement, this method can be used for product shape design and graphic software develop-ment.

关键词： curve interpolation B-spline subdivision scheme.

Extra-Large Seed Germplasms of Brassica Napus Created through Microspore Culture

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Journal of Environmental Science and Engineering(A) 2016年第2期5卷 90-96页

作者： Chen Wei Zhao Kaiqin Zhang Guojian Zhou Picai Zhao Desheng Qu Guan Luo Yangqing Zu Feng Li Jingfeng Dong Yunsong wang jingqiao Research Institute of Industrial Crops Academy of Agricultural Science Kunming 650205 China Department of Agriculture Yunnan Vocational and Technical College of Agriculture Kunming 650031 China Institute of Agricultural Promotion Academy of Agricultural Science Chuxiong 675000 China Research Institute of Oil Crops Academy of Agricultural Science Yuxi 653100 China

Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cross was made： H8--a Yunnan spring early-maturing rapeseed variety, ＂Legeney＂--a Canadian canola variety and ＂020010＂--a semi-winter late-maturing rapeseed variety. One hundred and forty eight doubled hyploid lines were obtained from the F~ plants of three-way cross through isolated mierospore culture. Among them, the TSW of GM01 amounted to 8.68 g and the TSW of 53 lines were above 5.0 g. The TSWs of GM01 were relatively stable among the multi-location field trials from 2007 to 2014 with variation being only 10%-15% among the locations and years. Compared with H8, GM01 had larger flowers, stigmas, siliques and seed diameters, but less branches, siliques per plant and seeds per silique.

关键词： Brassica napus germplasm seed weight morphological trait microspore culture.

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面向应急事件的卫星任务规划技术

航天返回与遥感 2022年第3期43卷 105-112页

作者：王静巧杨磊庄超然史小金中国四维测绘技术有限公司北京100094 中国资源卫星应用中心北京100094

陆地观测卫星在轨运控中,面向用户成像需求的任务规划通常分为常规和应急两种类型。常规任务规划即根据需求的类型及空间位置,结合卫星轨道信息进行需求过境计算及常规任务安排,以获取卫星影像数据;应急任务规划即通过技术手段实现卫星... 详细信息

陆地观测卫星在轨运控中,面向用户成像需求的任务规划通常分为常规和应急两种类型。常规任务规划即根据需求的类型及空间位置,结合卫星轨道信息进行需求过境计算及常规任务安排,以获取卫星影像数据;应急任务规划即通过技术手段实现卫星快速应急成像。文章通过分析卫星常规及应急运控模式,提出了卫星应急成像任务规划模式下需求统筹、卫星资源综合调度、应急可行性分析、测控和接收资源调度、应急计划快速调整等关键技术,总结了中国陆地观测卫星应急成像能力,对应急任务规划系统设计提出可行性建议,有效地提高了卫星应急时效性。

关键词：任务规划应急成像智能匹配动态任务规划陆地观测卫星

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高低轨遥感卫星联合监测火灾模式分析

航天器工程 2019年第6期28卷 101-105页

作者：王静巧车航宇史小金曾湧中国资源卫星应用中心

高低轨遥感卫星联合监测火灾模式是指使用高轨卫星每日针对重点区域巡查,发现火点,对已发现火点,快速调动高轨卫星应急规划,高频次获取灾区观测数据,掌握火势发展情况;通过低轨高分辨卫星侧摆成像,详查灾害区域,进行过火面积评估。文章... 详细信息

高低轨遥感卫星联合监测火灾模式是指使用高轨卫星每日针对重点区域巡查,发现火点,对已发现火点,快速调动高轨卫星应急规划,高频次获取灾区观测数据,掌握火势发展情况;通过低轨高分辨卫星侧摆成像,详查灾害区域,进行过火面积评估。文章针对遥感卫星高低轨联合监测过程中卫星数据获取时效性存在的问题及要求,建立高轨卫星一键式应急任务规划系统,并构建森林火灾应急需求与低轨卫星资源匹配的规则和算法,实现应急任务一键式分配至各低轨卫星任务规划系统,提升高低轨卫星应急响应时间。

关键词：森林火灾高轨卫星低轨卫星联合观测

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中国陆地观测卫星应急成像时效性分析

航天返回与遥感 2022年第4期43卷 111-122页

作者：史小金王静巧庄超然曾湧中国四维测绘技术有限公司北京100094 中国资源卫星应用中心北京100094

为研究中国陆地观测卫星全球应急成像能力,通过分析卫星应急任务规划中涉及的卫星资源、测控资源及数传资源等,综合卫星过境窗口、卫星载荷能力、应急指令执行能力,提出了中国陆地观测卫星应急成像时效性分析方法;对境内及周边国家、欧... 详细信息

为研究中国陆地观测卫星全球应急成像能力,通过分析卫星应急任务规划中涉及的卫星资源、测控资源及数传资源等,综合卫星过境窗口、卫星载荷能力、应急指令执行能力,提出了中国陆地观测卫星应急成像时效性分析方法;对境内及周边国家、欧洲及非洲、南北美洲等地区的应急成像与应急影像数据接收时效性进行分析计算,相关结果紧贴中国陆地观测卫星的测控能力、测控资源、数传资源的实际现状,可为中国陆地观测卫星的测控和数传资源的发展建设提供良好依据。

关键词：陆地观测卫星星地资源应急能力应急时效性

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几个Bézier、有理Bézier曲线性质的算子化证明

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河海大学学报（自然科学版） 1999年第4期27卷 12-15页

作者：陈正鸣王锦桥河海大学常州分校常州213022

利用Ｂéｚｉｅｒ、有理Ｂéｚｉｅｒ曲线的算子表示，非常简捷地证明了Ｂéｚｉｅｒ曲线和有理Ｂéｚｉｅｒ曲线的分段性和包络性．类似的方法很容易推广到Ｂéｚｉｅｒ、有理Ｂéｚｉｅｒ曲面上．

关键词： CAGD 算子 Bézier曲线有理Bézier曲线

伴前庭水管扩大的非综合征性听力障碍耳聋基因突变谱研究

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中国康复理论与实践 2023年第1期29卷 119-124页

作者：王屹陈振波李勇王晶俏刘志忠中国康复研究中心北京博爱医院检验科北京市100068 中国康复研究中心北京博爱医院影像科北京市100068 首都医科大学康复医学院北京市100068 中国医学科学院整形外科医院放射科北京市100144

目的观察伴前庭水管扩大(EVA)的非综合征性听力障碍(NSHI)患者耳聋基因突变谱。方法2015年10月至2016年8月,对湖北宜昌特殊教育学校确诊为NSHI的患者85例进行颞骨CT检查,基质辅助激光解吸/电离飞行时间质谱对GJB2、GJB3、SLC26A4和线粒... 详细信息

目的观察伴前庭水管扩大(EVA)的非综合征性听力障碍(NSHI)患者耳聋基因突变谱。方法2015年10月至2016年8月,对湖北宜昌特殊教育学校确诊为NSHI的患者85例进行颞骨CT检查,基质辅助激光解吸/电离飞行时间质谱对GJB2、GJB3、SLC26A4和线粒体DNA(mtDNA)12S rRNA的20个耳聋相关基因突变位点进行检测。结果颞骨CT显示EVA患者31例。与非EVA患者相比,EVA患者携带突变的比例明显增高(χ^(2)=11.160,P=0.001),主要表现为SLC26A4基因中c.919-2A>G突变水平显著增高(χ^(2)=23.870,P<0.001)。结论伴EVA的NSHI患者耳聋基因突变谱不同于非EVA患者,可优化耳聋基因检测方案,为伴EVA的NSHI的早诊断、早干预提供帮助。

关键词：非综合征性听力障碍前庭水管扩大基因突变