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Science China(Life Sciences) 2015年第2期58卷 178-186页

作者： tong jingou SUN XiaoWen State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Heilongjiang Fisheries Research Institute Chinese Academy of Fishery Sciences

The traits of cultured fish must continually be genetically improved to supply high-quality animal protein for human *** important fish traits are controlled by multiple gene quantitative trait loci(QTL),most of which have minor effects,but a few genes may have major effects useful for molecular *** this review,we chose relevant studies on some of the most intensively cultured fish and concisely summarize progress on identifying and verifying QTLs for such traits as growth,disease and stress resistance and sex in recent *** potential applications of these major-effect genes and their associated markers in marker-assisted selection and molecular breeding,as well as future research directions are also *** genetic and genomic analyses will be valuable for elucidating the mechanisms modulating economically important traits and to establish more effective molecular breeding techniques in fish.

关键词： aquaculture fish economic traits QTL major genes molecular markers molecular breeding

Construction of the first high-density genetic map for growth related QTL analysis in Ancherythroculter nigrocauda

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Journal of Oceanology and Limnology 2021年第3期39卷 1118-1130页

作者： Yanhong SUN Pei LI Guiying WANG Renli SUN Jian CHEN Qi ZHOU jingou tong Qing LI Fisheries Research Institute Wuhan Academy of Agricultural SciencesWuhan 430207China Wuhan Xianfeng Aquaculture Technology Co.Ltd. Wuhan 430207China Wuhan Academy of Agricultural Sciences Wuhan 430207China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China

Ancherythroculter nigrocauda is a fish endemic to the upper areas of the Changjiang(Yangtze)River in *** trait locus(QTL)mapping is a powerful tool to identify potential genes affecting traits of economic importance in domestic *** this study,a high-density genetic map was constructed with 5901 single nucleotide polymorphism(SNP)makers by sequencing 92 individual fish from a F1 family using the specific-locus amplified fragment sequencing ***,48 QTLs for total length,body length,body height,and body weight were identified according to the high density of the genetic map with 24 LGs,a total length of 3839.4 cM,and marker spacing of about 0.82 *** QTLs explained 27.1%-49.9%of phenotypic *** results of this study suggest that major QTLs are responsible for the growth of ***,and these are potentially useful in comparative genomics research,genome assembly,and marker-assisted breeding programs for this species.

关键词： Ancherythroculter nigrocauda specific-locus amplified fragment high-density genetic map quantitative trait locus

Characterization of stmn1a and genetic effects of a 22-bp Indel in its promoter region of bighead carp Hypophthalmichthys nobilis

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Water Biology and Security

Water Biology and Security 2024年第2期3卷 73-82页

作者： Xiaoyu Zhou Yanhong Wu Junru Wang Xiaomu Yu jingou tong State Key Laboratory of Freshwater Ecology and Biotechnology Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture of CASHubei Hongshan LaboratoryInnovation Academy of Seed DesignInstitute of HydrobiologyChinese Academy of SciencesWuhan430072China College of Fisheries Xinyang Agriculture and Forestry UniversityXinyang464399China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing100049China

Stathmin 1(stmn1)gene,which is involved in the control of cell cycle progression,cell proliferation,differentiation and motility,is a crucial relay in the signal transduction of multiple signaling *** on the function of stmn1 in fish are *** this study,the characterization and function of stmn1a gene were explored in an important food fish,namely bighead carp(Hypophthalmichthys nobilis).The bighead carp stmn1a(Hynstmn1a)genomic sequence,which has 2995 bp with an ORF of 447 bp,is evolutionarily conserved in teleost *** highest level of expression of Hynstmn1a is in the un-fertilized eggs and spleen of adult bighead *** overexpression of Hynstmn1a facilitates cell cycle progression and suppresses apoptosis in EPC *** an siRNA assay,disruption of stmn1a in EPC cells resulted in the inhibition of cell cycle progression and promotion of *** of promoter activity indicated that an upstream transcription factor(foxo4)regulates *** mutants with stmn1a knockout by CRISPR/Cas9 exhibited a significant retardation of body length(p<0.05).There was no significant difference in body weight between stmn1a-mutant zebrafish and wildtype(p=0.053).A 22-bp Indel in the promoter region of Hynstmn1a with an observed heterozygosity(Ho)of 0.136 in a bighead carp population(n=191).The polymorphism of this 22-bp Indel genotype is significantly associated with body length(BL),head length(HL)(p<0.05),body weight(BW),head height(HH),and head width(HW)(p<0.01),showing its genetic effects on growth *** study sheds light on the function of stmn1a in fish and demonstrates the potential of the 22-bp Indel of Hynstmn1a as a gene marker when selecting breeding individuals for faster growth and a bigger head of bighead carp in aquaculture.

关键词： Indel Functional analyses Growth-related traits Hypophthalmichthys nobilis stmn1a

Genomic polymorphisms at the crhr2 locus improve feed conversion efficiency through alleviation of hypothalamus-pituitary-interrenal axis activity in gibel carp(Carassius gibelio)

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Science China(Life Sciences) 2022年第1期65卷 206-214页

作者： Chuang Shi Qiyong Lou Beide Fu Junyan Jin Jianfei Huang Yao Lu Xia Jin Jiangyan He Gang Zhai Shouqi Xie jingou tong Jianfang Gui Zhan Yin State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100864China

Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish ***,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain ***,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress ***,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries.

关键词： feed conversion efficiency crhr2 stress responses endocrine regulation genetic marker for fish breeding

Microsatellite Development for an Endangered Bream Megalobrama pellegrini(Teleostei,Cyprinidae)Using 454 Sequencing

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Microsatellite Development for an Endangered Bream Megalobra...

鳙bmi1b基因单核苷酸多态性及其与生长和体型性状的关联性

2012年中国西部动物学首届学术研讨会

作者： WANG Jinjin YU Xiaomu ZHAO Kai ZHANG Yaoguang tong jingou PENG Zuogang Key Laboratory of Freshwater Fish Reproduction and Development(Ministry of Education) Key Laboratory of Aquatic Science of ChongqingSchool of Life ScienceSouthwest University State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences Key Laboratory of Adaptation and Evolution of Plateau Biota(AEPB) Northwest Plateau Institute of BiologyChinese Academy of Sciences

Megalobrama pellegrini is an endemic fish species found in the upper Yangtze River basin in *** species has become endangered due to the construction of the Three Gorges Dam and ***,the available genetic data for this species is ***,we developed 26 polymorphic microsatellite markers from the *** genome using next-generation sequencing techniques.A total of 257,497 raw reads were obtained from a quarter-plate run on 454 GS-FLX titanium platforms and 49,811 unique sequences were generated with an average length of 404 bp;24,522(49.2%)sequences contained microsatellite *** the 53 loci screened,33 were amplified successfully and 26 were *** genetic diversity in *** was evaluated in a population level with 30 samples,which showed a moderate genetic diversity in *** with an average of 3.08 alleles per locus,and the mean observed and expected heterozygosity were0.47 and 0.51,*** addition,we tested cross-species amplification for all 33 loci in four additional breams:***,***,***,and Sinibrama wui,each with five *** cross-species amplification showed a significant high level of transferability(79%-97%),which might be due to their dramatically close genetic *** polymorphic microsatellites developed in the current study will not only contribute to further conservation genetic studies and parentage analyses of this endangered species,but also facilitate future work on the other closely related species.

关键词： high-throughput microsatellite isolation next-generation sequencing breams cross-amplification

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中国水产科学 2022年第4期29卷 503-514页

作者：高一凡俞小牧桂建芳童金苟大连海洋大学水产与生命学院淡水生态与生物技术国家重点实验室湖北洪山实验室中国科学院种子创新研究院中国科学院水生生物研究所

bmi1b基因作为转录抑制因子,在维持多种干细胞的自我更新和增殖活性方面起重要作用。本研究对鳙(Hypophthalmichthys nobilis) bmi1b基因进行了单核苷酸多态性发掘及其与生长和体型性状的关联性分析。鳙bmi1b全长5800 bp,包含9个外显子... 详细信息

bmi1b基因作为转录抑制因子,在维持多种干细胞的自我更新和增殖活性方面起重要作用。本研究对鳙(Hypophthalmichthys nobilis) bmi1b基因进行了单核苷酸多态性发掘及其与生长和体型性状的关联性分析。鳙bmi1b全长5800 bp,包含9个外显子、8个内含子,其氨基酸序列在进化上较为保守。bmi1b在鳙下丘脑和肝脏中的表达量较高,胚胎发育阶段从未受精卵至囊胚期都有极高的表达,原肠期后表达量显著降低,具有母源基因的表达特征。采用PCR扩增产物直接测序的方法,在鳙bmi1b 3′UTR获得2个SNPs g.5224 T>A和g.5550 C>T。利用来源于1个鳙混合群体的169尾鱼进行SNP基因分型及其与生长和体型性状的相关性分析,结果表明:g.5224T>A与体重和头高呈显著相关(PT与体重和体型性状的相关性未达到显著水平。2个SNP位点等位基因间的组合分析显示,双倍型D2(ATCC)为优势基因型组合,其体重和体型性状的平均值显著高于其他双倍型。这些结果为进一步研究鱼类bmi1b基因的功能提供了参考;同时鳙bmi1b基因SNP标记在生长和体型性状的分子育种研究中也具有良好的应用潜力。

关键词：鳙 bmi1b 单核苷酸多态性生长性状体型关联性分析

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黄河鲤全同胞家系的微卫星标记亲子鉴定

中国水产科学 2016年第5期23卷 1023-1031页

作者：王新华俞小牧冯建新童金苟中国科学院水生生物研究所淡水生态与生物技术国家重点实验室中国科学院大学河南省水产科学研究院

本研究利用11个高度多态的微卫星标记对26个黄河鲤(Cyprinus carpio haematoperus)全同胞家系中的417尾体重较大的正常体色个体(黄河鲤较大个体)和52尾红色个体(红鲤表型个体)进行了亲子鉴定。11个微卫星座位的平均等位基因数、平均观... 详细信息

本研究利用11个高度多态的微卫星标记对26个黄河鲤(Cyprinus carpio haematoperus)全同胞家系中的417尾体重较大的正常体色个体(黄河鲤较大个体)和52尾红色个体(红鲤表型个体)进行了亲子鉴定。11个微卫星座位的平均等位基因数、平均观测杂合度(H)、平均期望杂合度(H)和平均多态信息含量(PIC)分别为8.2、0.792、0.792和0.76。当双亲未知且置信度为95%时,11个座位的累积排除概率达99.79%。除29尾黄河鲤较大个体以外,388尾黄河鲤较大个体和52尾红鲤表型个体准确地找到了其父母本,实际鉴定率为93.82%。通过对子代数目超过20尾的7个黄河鲤家系生长性状的比较分析,本研究成功鉴定出子代生长性状优良的黄河鲤亲本组合及其家系,同时也鉴定出包含隐性红色基因的黄河鲤杂合亲本,为进一步选育表型和体色纯正且生长快速的黄河鲤新品种提供了理论依据和技术手段。

关键词：黄河鲤亲子鉴定微卫星鉴定率

团头鲂性腺发育不同时期组织学观察及Kiss2/Kiss2r基因表达分析

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南方水产科学 2013年第3期9卷 44-50页

作者：温久福高泽霞罗伟童金苟王卫民华中农业大学水产学院农业动物遗传育种与繁育教育部重点实验室农业部淡水生物繁育重点实验室湖北武汉430070 中国科学院水生生物研究所淡水生态与生物技术国家重点实验室湖北武汉430072

Kiss基因(Kisspeptin)及其受体Kissr基因(Kiss receptor)组成的系统参与下丘脑-垂体-性腺轴功能的调节,在脊椎动物青春期启动的过程中发挥重要作用。该研究通过组织切片技术观察了团头鲂(Megalobrama amblyceph-ala)卵巢和精巢的不同分... 详细信息

Kiss基因(Kisspeptin)及其受体Kissr基因(Kiss receptor)组成的系统参与下丘脑-垂体-性腺轴功能的调节,在脊椎动物青春期启动的过程中发挥重要作用。该研究通过组织切片技术观察了团头鲂(Megalobrama amblyceph-ala)卵巢和精巢的不同分期,进一步采用荧光定量PCR技术分析比较了团头鲂Kiss2和Kiss2r基因在雌、雄性腺不同时期对应的脑、垂体、性腺组织中的表达量。组织切片观察明确鉴别出团头鲂卵巢和精巢发育Ⅰ期至Ⅵ期的性腺组织结构,基因荧光定量PCR分析结果表明Kiss2/Kiss2r系统主要是通过增加团头鲂卵巢发育Ⅱ期时脑和垂体的表达量以及Ⅲ期精巢的表达量来发挥生殖启动作用。

关键词：团头鲂 Kiss2 Kiss2r系统性腺发育基因表达

金沟河二级水电站引水渠首设计与排沙措施研究

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广东水利水电 2019年第5期 13-16页

作者：童亮新疆水利水电勘测设计研究院

金沟河为多泥沙河流,径流主要靠冰川、永久性积雪的消融和夏季降水补给,河道水量小,河床多为砂砾石阶地,厚度可达数十米。该文介绍金沟河二级水电站引水渠首设计及泥沙处理措施,可为类似工程设计提供借鉴。

关键词：渠首前池排沙漏斗金沟河