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Response of bacterial communities to short-term pyrene exposure in red soil

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Frontiers of Environmental Science & Engineering 2013年第4期7卷 559-567页

作者： jingjing peng Hong LI Jianqiang SU Qiufang ZHANG Junpeng RUI Chao CAI Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China Lancaster Environment Centre Lancaster University Lancaster LA1 4YQ UK College of Resources and Environmental Sciences China Agricultural University Beijing 100193 China

Pyrene, a representative polycyclic aromatic hydrocarbon （PAH） compound produced mainly from incomplete combustion of fossil fuels, is hazardous to ecosystem health. However, long-term exposure studies did not detect any significant effects of pyrene on soil microorganism. In this study, short-term microcosm experiments were conducted to identify the immediate effect of pyrene on soil bacterial communities. A freshly- collected pristine red soil was spiked with pyrene at 0, 10, 100, 200, and 500 ***-~ and incubated for one day and seven days. The bacterial communities in the incubated soils were analyzed using 16S rRNA sequencing and terminal restriction fragment length polymorphism （T- RFLP） methods. The results revealed high bacterial diversity in both unspiked and pyrene-spiked soils. Only at the highest pyrene-spiking rate of 500 ***-~, two minor bacteria groups of the identified 14 most abundant bacteria groups were completely suppressed. Short-term exposure to pyrene resulted in dominance of Proteobac- teria in soil, followed by Acidobacteria, Firmutes, and Bacteroidetes. Our findings showed that bacterial commu- nity structure did respond to the presence of pyrene but recovered rapidly from the perturbation. The intensity of impact and the rate of recovery showed some pyrene dosage-dependent trends. Our results revealed that differ- ent levels of pyrene may affect the bacterial community structure by suppressing or selecting certain groups of bacteria. It was also found that the bacterial community was most susceptible to pyrene within one day of the chemical addition.

关键词： pyrene bacterial communities terminalrestriction fragment length polymorphism short-term

Soil microbial ecology through the lens of metatranscriptomics

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Soil Ecology Letters 2024年第3期6卷 1-15页

作者： jingjing peng Xi Zhou Christopher Rensing Werner Liesack Yong-Guan Zhu State Key Laboratory of Nutrient Use and Management College of Resources and Environmental SciencesNational Academy of Agriculture Green DevelopmentKey Laboratory of Plant-Soil InteractionsMinistry of EducationChina Agricultural UniversityBeijing 100193China Institute of Environmental Microbiology College of Resource and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China Max Planck Institute for Terrestrial Microbiology Marburg 35043Germany State Key Laboratory of Urban and Regional Ecology Research Centre for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China

Metatranscriptomics is a cutting-edge technology for exploring the gene expression by,and functional activities of,the microbial community across diverse ecosystems at a given time,thereby shedding light on their metabolic responses to the prevailing environmental *** double-RNA approach involves the simultaneous analysis of rRNA and mRNA,also termed structural and functional *** contrast,mRNA-centered metatranscriptomics is fully focused on elucidating community-wide gene expression profiles,but requires either deep sequencing or effective rRNA *** this review,we critically assess the challenges associated with various experimental and bioinformatic strategies that can be applied in soil microbial ecology through the lens of functional *** particular,we demonstrate how recent methodological advancements in soil metatranscriptomics catalyze the development and expansion of emerging research fields,such as rhizobiomes,antibiotic resistomes,methanomes,and *** review provides a framework that will help to design advanced metatranscriptomic research in elucidating the functional roles and activities of microbiomes in soil ecosystems.

关键词： metatranscriptomics mRNA MAGs rhizobiont resistome virome

Beyond microbial carbon use efficiency

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National Science Review 2024年第4期11卷 33-35页

作者： Ke-Qing Xiao Chao Liang Zimeng Wang jingjing peng Yao Zhao Ming Zhang Mingyu Zhao Shuiqing Chen Yong-Guan Zhu Caroline L.Peacock State Key Lab of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of Sciences University of Chinese Academy of Sciences Institute of Applied Ecology Chinese Academy of Sciences Department of Environmental Science and Engineering Fudan University College of Resources and Environmental Sciences China Agricultural University State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences State Key Laboratory of Agricultural Microbiology College of Resources and Environment Huazhong Agricultural University Key Laboratory of Cenozoic Geology and Environment Institute of Geology and Geophysics Chinese Academy of Sciences School of Earth and Environment University of Leeds

Microbial carbon use efficiency (CUE)is defined as the proportion of microbial biomass growth C versus substrate C uptake, and thus provides a useful measure of microbially driven accumulation and loss of soil organic carbon (SOC)[1]. In a recent study published in Nature [2],the authors used a data-driven machinelearning approach to conclude that CUE promotes global SOC storage based on a positive correlation between CUE and SOC content and that, based on sensitivity analysis, CUE is at least four times as important as six other evaluated factors,namely plant C inputs, C input allocation, non-microbial C transfer, substrate decomposability, environmental modifications and vertical transport.

关键词： beyond carbon efficiency microbial

OPPORTUNITIES AND APPROACHES FOR MANIPULATING SOIL-PLANT MICROBIOMES FOR EFFECTIVE CROP NITROGEN USE IN AGROECOSYSTEMS

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Frontiers of Agricultural Science and Engineering 2022年第3期9卷 333-343页

作者： jingjing peng Olatunde OLADELE Xiaotong SONG Xiaotang JU Zhongjun JIA Hangwei HU Xuejun LIU Shuikuan BEI Anhui GE Limei ZHANG Zhenling CUI College of Resources and Environmental Sciences National Academy of Agriculture Green DevelopmentKey Laboratory of Plant-Soil InteractionsMinistry of EducationChina Agricultural UniversityBeijing 100193China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Tropical Crops Hainan UniversityHaikou 570228China Key Labortatory of Mollisols Agroecology Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China Faculty of Veterinary and Agricultural Sciences The University of MelbourneParkvilleVIC.3010Australia University of Chinese Academy of Sciences Beijing 100049China.

Soil microbiomes drive the biogeochemical cycling of nitrogen and regulate soil N supply and loss,thus,pivotal nitrogen use efficiency(NUE).Meanwhile,there is an increasing awareness that plant associated microbiomes and soil food web interactions is vital for modulating crop productivity and N *** rapid advances in modern omics-based techniques and biotechnologies make it possible to manipulate soil-plant microbiomes for improving NUE and reducing N environmental *** paper summarizes current progress in research on regulating soil microbial N cycle processes for NUE improvement,plant-microbe interactions benefiting plant N uptake,and the importance of soil microbiomes in promoting soil health and crop *** also proposes a potential holistic(rhizosphere-root-phyllosphere)microbe-based approach to improve NUE and reduce dependence on mineral N fertilizer in agroecosystems,toward nature-based solution for nutrient management in intensive cropping systems.

关键词： nitrogen microbiome NUE rhizosphere phyllosphere soil food web

Analysis on the Cause and Forecast Deviation of an Extreme Rainstorm in Changsha Urban Area

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Meteorological and Environmental Research 2023年第1期14卷 50-55,59页

作者： Yun FANG Tianyao SHEN Sheng ZHOU jingjing peng Lijun CHENG Changsha Meteorological Bureau Changsha 410205China Hunan Provincial Weather Modification Leading Group Office Changsha 410118China

Using NCEP reanalysis data,high-altitude and ground observation data,numerical model data,satellite and radar data,formation cause and forecast deviation of an extreme rainstorm process in Changsha urban area at night on June 9,2020 were *** results showed that(1)the extreme rainstorm process developed near the surface convergence line,with strong localization,short duration and large hourly rainfall intensity.(2)Under the high temperature and high humidity environment,the low-level cold advection and the hot low-pressure system interacted,and the potential con-vective unstable energy was released,and a strong convective weather was formed.(3)The convergence of water vapor in the lower layer and the strong upward movement provided sufficient water vapor for the *** low-centroid thunderstorm was the main reason for the extreme rainstorm.(4)The forecast deviation of the numerical model to the low-level shear line and the mesoscale convergence line was an important reason for the forecast deviation of the heavy rainfall area.

关键词： Extreme rainstorm Ground convergence line Unstable energy

Fasudil hydrochloride differentiates bone marrow mesenchymal stem cells into neurons via notch signaling

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Neural Regeneration Research 2010年第11期5卷 814-819页

作者： Eryi Zhao Liudong Wang Quanqing Wen Wenjuan Guan jingjing Lu Tao peng Boai Zhang Yanjie Jia Department of Neurology First Affiliated Hospital Zhengzhou University Zhengzhou 450052 Henan Province China

BACKGROUND： Notch signaling regulates bone marrow mesenchymal stem cell （MSC） proliferation, differentiation, and apoptosis, Notch signaling and Rho kinase signaling exhibit a crosstalk phenomenon with JAK/STAT, and both participate in the neuronal dendritic spine development. Inhibition of RhoA/Rho kinase signaling may regulate MSC differentiation into neuronal-like cells. OBJECTIVE： To investigate the effect of Notch1 signaling on the differentiation of rat MSCs into neurons induced by fasudil hydrochloride （C14H17N3O2S-HCI）, a Rho kinase inhibitor, through a siRNA approach. DESIGN, TIME AND SETTING： An in vitro cytological experiment was performed in the Cell Laboratory of Henan Academy of Medical and Pharmaceutical Sciences between December 2007 and May 2009. MATERIALS： MSCs were obtained from Wistar rat femoral bone, fasudil hydrochloride was provided by -Tianjin Chase Sun Pharmaceutical Co., Ltd. Rn-notchl-siRNa, negative control siRNA （Cy3 label） and Rn-MAPK1 control siRNA were provided by QIAGEN, Coloqne, German. METHODS： The cultured MSCs were divided into non-transfected, transfected group （transfected with Rn-Notchl-siRNA）, positive control （transfected with Rn-MAPK-1 control siRNA）, and negative control （transfected with negative control siRNA） groups. Fasudil hydrochloride was applied to induce MSCs to differentiate into neurons. MAIN OUTCOME MEASURES： The fluorescence expression by the transfected MSCs was observed under an inverted fluorescence microscope; the expression of Notch1 mRNA, Hesl mRNA, and MAPK1 mRNA in MSCs was detected by reverse transcription polymerase chain reaction; the expression of Notch1 protein, nestin, neurofilament M, and glial fibrillary acidic protein was detected by immunocytochemistry. The viability of MSCs was detected by tetrazolium bromide assay. RESULTS： MSC fluorescence increased following a 72-hour siRNA transfection, with transfection efficiencies of up to （0.91 ± 0.04）; the Notch1 mRNA and Hesl mRNA expressed by tra

关键词： Notch Rho fasudil hydrochloride marrow mesenchymal stem cells neuronal-like cells transfection nerve stem cells neural regeneration

Multi-scale validation strategy for satellite albedo products and its uncertainty analysis

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Science China Earth Sciences 2015年第1期58卷 573-588页

作者： peng jingjing LIU Qiang WEN JianGuang LIU QinHuo TANG Yong WANG LiZhao DOU BaoCheng YOU DongQin SUN ChangKui ZHAO XiaoJie FENG YouBin SHI Jian State Key Laboratory of Remote Sensing Science Institute of Remote Sensing and Digital Earth of Chinese Academy of Sciences College of Global Change and Earth System Science Beijing Normal University

Coarse-resolution satellite albedo products are important for climate change and energy balance research because of their capability to characterize the spatiotemporal patterns of land surface parameters at both the regional and global scales. The accuracy of coarse-resolution products is usually assessed via comparison with in situ measurements. The key issue in the comparison of remote sensing observations with in situ measurements is scaling and uncertainty. This paper presents a strategy for validating 1-km-resolution remote sensing albedo products using field measurements and high-resolution remote sensing observations. Field measurements were collected to calibrate the high-resolution(30 m) albedo products derived from HJ-1a/b images. Then, the calibrated high-resolution albedo maps were resampled(i.e., upscaled) to assess the accuracy of the coarse-resolution albedo products. The samples of field measurements and high-resolution pixels are based on an uncertainty analysis. Two types of coarse-resolution albedo datasets, from global land surface satellite(GLASS) and moderate-resolution imaging spectroradiometer(MODIS), are validated over the middle reaches of the Heihe River in China. The results indicate that the upscaled HJ(Huan Jing means environment in Chinese and this refers to a satellite constellation designed for environment and disaster monitoring by China) albedo, which was calibrated using field measurements, can provide accurate reference values for validating coarse-resolution satellite albedo products. However, the uncertainties in the upscaled HJ albedo should be estimated, and pixels with large uncertainties should be excluded from the validation process.

关键词： MCD43B3 GLASS02 uncertainty analysis validation remote sensing albedo

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出血性卒中患者早期活动安全性的研究进展

护理学杂志 2024年第22期39卷 121-125页

作者：彭茜彭静静重庆医科大学附属第一医院神经外科

从出血性卒中患者早期活动定义、安全活动方案和安全评估3个方面对国内外研究现状及进展进行综述，旨在为出血性卒中患者正确制订诊疗护理计划、保障临床护理实践的安全实施提供参考。

关键词：出血性卒中自发性脑出血动脉瘤性蛛网膜下腔出血早期活动活动方案评估方法康复护理综述文献

矩阵不等式约束下矩阵方程AX=B的双对称解

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计算数学 2013年第2期35卷 137-150页

作者：李姣芬彭振赟彭靖静桂林电子科技大学数学与计算科学学院广西桂林541004

本文讨论矩阵不等式CXD≥E约束下矩阵方程AX=B的双对称解,即给定矩阵A,B,C,D和E,求双对称矩阵X,使得AX=B和CXD≥E,其中CXD≥E表示矩阵CXD-E非负.本文将问题转化为矩阵不等式最小非负偏差问题,利用极分解理论给出了求其解的迭代方法,并... 详细信息

本文讨论矩阵不等式CXD≥E约束下矩阵方程AX=B的双对称解,即给定矩阵A,B,C,D和E,求双对称矩阵X,使得AX=B和CXD≥E,其中CXD≥E表示矩阵CXD-E非负.本文将问题转化为矩阵不等式最小非负偏差问题,利用极分解理论给出了求其解的迭代方法,并结合相关矩阵理论说明算法的收敛性.最后给出数值算例验证算法的有效性.

关键词：矩阵不等式矩阵方程双对称矩阵迭代法极分解