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Genes & Diseases 2023年第6期10卷 2233-2236页

作者： Xiangjun Liu jingang gui Xiaolin Wang Laboratory of Tumor Immunology Beijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's Health(NCCH)Beijing 100045China

Severe acute respiratory syndrome coronavirus-2(SARSCoV-2),which causes coronavirus disease 2019(COVID-19)and initially infects epithelial cells of the upper respiratory tract,has recently caused a global health emergency and is still a potential threat to humans.1 Multiple studies have suggested alterations in the immune system,including excessive neutrophil activation and lymphopenia,and excessive inflammatory responses as the key mechanisms for severesymptoms.2However,which cell types contribute to excessive inflammation remains to be *** analysis of human blood immune cells provides insights into the coordinated response to SARSCoV-2 infections and is a significant task for restraining the virus and controlling disease progression.

关键词： neutrophil respiratory alterations

Spontaneous NETosis and type I IFN signaling activation in resting neutrophils of chronic granulomatous disease patients with CYBB mutations

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Genes & Diseases 2024年第6期11卷 8-11页

作者： Zhengjing Lu Wenjun Mou Jianxin He Xuedong Ge Xiaolin Wang jingang gui Laboratory of Tumor Immunology Key Laboratory of Major Diseases in ChildrenMinistry of EducationBeijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijing 100045China Department of Respiratory Medicine Beijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthChina National Clinical Research Center for Respiratory DiseasesBeijing 100045China Information Center PLA Rocket Force Characteristic Medical CenterBeijing 100088China

Mutations in CYBB,encoding gp91^(phox)subunit of NADPH oxidase in phagocytes,impair the respiratory burst of neutrophils and result in X-linked chronic granulomatous disease(CGD).While inflammatory response and NETosis are important modalities employed by neutrophils for pathogen clearance,variants in these cell functions in CGD neutrophils(CGD-PMN)could possibly explain the insufficient defense and accumulation of phagocytes in the sites of infection.

关键词： neutrophils granuloma respiratory

Cell-mediated immunity to SARS-CoV-2

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Pediatric Investigation 2020年第4期4卷 281-291页

作者： Xiaolin Wang jingang gui Laboratory of Tumor Immunology Beijing Pediatric Research InstituteBeijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)viruses spread unscrupulously virtually every corner on the planet in a very quick speed leading to an unprecedented world pandemic of COVID-19 claiming a great many of people’s *** importance has been given to the studies on the virus itself including genomic variation and viron structure,as well as cell entry pathway and tissue *** than that,to learn the main characteristic of host immunity responding to SARS-CoV-2 infection is an eminent task for restraining virus and controlling disease *** antibody production in response to SARS-CoV-2 infection,host cellular immunity plays an indispensable role in impeding virus replication and expansion at various stages of COVID-19 *** this review,we summarized the recent knowledge regarding the aberrant regulation and dysfunction of multiple immune cells during SARS-CoV-2 *** includes the dysregulation of immune cell number,Th polarity,cytokine storm they implicated with,as well as cell function exhaustion after chronic virus *** that many obstacles remain to be overcome,studies on immunotherapy for COVID-19 treatment based on the known features of host immunity in response to SARS-CoV-2 infection offer us tangible benefits and hope for making this SARS-CoV-2 pandemic under control.

关键词： SARS-CoV-2 COVID-19 Innate immune T-cell exhaustion Cytokine storm Immunotherapy

Severe cases of BCGosis-susceptible primary immunodeficiency diseases identified by next-generation sequencing:Implications for adjustment of BCG vaccination timing in China

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Journal of Genetics and Genomics 2020年第4期47卷 229-232页

作者： Gang Liu Haijuan Xiao Linlin Liu Lingyun Guo Ruolan Guo Xuyun Hu Chanjuan Hao jingang gui Weiwei Jiao Fang Xu Adong Shen Wei Li Department of Infectious Disease Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijing 100045China Beijing Pediatric Research Institute Genetics and Birth Defects Control CenterBeijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijing 100045China

The World Health Organization(WHO)recommends neonatal Bacillus Calmette-Guerin(BCG)vaccination in countries with a high prevalence of tuberculosis(TB),which has been practiced in more than 150 countries(WHO,2004).The prevalence of TB is a serious condition in China,ranking as the second in the high epidemic countries globally(WHO,2018).China initiated a neonatal BCG vaccination program in 1986 and reached an average vaccination rate of 99%in 2016 with a great success in preventing the incidence of TB(WHO,2018).

关键词： vaccination neonatal diseases

DCX and CRABP2 are candidate genes for differential diagnosis between pre-chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients

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Pediatric Investigation 2021年第2期5卷 106-111页

作者： Nian Sun Yeran Yang Shengcai Wang Jie Zhang jingang gui Jun Tai Lejian He Jiatong Xu Yanzhen Li Xuexi Zhang Qiaoyin Liu Zhiyong Liu Yongli Guo Xin Ni Department of Otolaryngology Head and Neck SurgeryBeijing Children's HospitalCapital Medical UniversityNational Center for Children's HealthBeijingChina Beijing Key Laboratory for Pediatric Diseases of Otolaryngology Head and Neck SurgeryMOE Key Laboratory of Major Diseases in ChildrenBeijing Pediatric Research InstituteBeijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina Laboratory of Tumor Immunology Beijing Pediatric Research InstituteBeijing Children's HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina Department ofOtorhinolaryngology Children’s HospitalCapital Institute of PediatricsBeijingChina Department of Pathology Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina

Importance Rhabdomyosarcoma(RMS)is the most common soft tissue sarcoma in *** than 90%of cases are classified as embryonic RMS(ERMS)or alveolar RMS(ARMS).ERMS has a worse prognosis than *** differential diagnosis is of paramount importance for optimization of *** To identify genes that are differentially expressed between ARMS and ERMS,which can be used for accurate rhabdomyosarcoma *** Three Gene Expression Omnibus datasets composed of ARMS and ERMS samples were screened and 35 differentially expressed genes(DEGs)were *** operating characteristic curve analysis and area under the curve analysis was performed for these 35 DEGs and seven candidate genes with the best differential expression scores between ARMS and ERMS were *** expression of these seven candidate genes was validated by immunohistochemical analysis of pre-chemotherapy ARMS and ERMS *** The levels of DCX and CRABP2 were confirmed to be remarkably different between paraffin-embedded ARMS and ERMS tissues,while EGFR abundance was only marginally different between these two RMS *** DCX and CRABP2 are potential biomarkers for distinguishing ARMS from ERMS in pre-chemotherapy pediatric patients.

关键词： Alveolar rhabdomyosarcoma Embryonic rhabdomyosarcoma DCX CRABP2 Immunohistochemistry

自建气动阀式微米按需液滴喷射系统及对细胞活性的影响

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北京工业大学学报 2017年第4期43卷 496-501页

作者：王志海聂志雄王梦仝帅陈曦桂晋刚北京工业大学信息学部北京100124 北京工业大学激光工程研究院北京100124 首都医科大学附属北京儿童医院北京儿科研究所北京100035

为了喷射细胞并保持高的细胞活性,设计并搭建了一套基于气动阀控技术的微液滴按需喷射装置.该装置主要部件包括储液腔体、位于腔体底部的100μm直径喷嘴、以高速电磁阀和放气管为核心的气路以及液滴拍照系统.该装置利用高速电磁阀产生... 详细信息

为了喷射细胞并保持高的细胞活性,设计并搭建了一套基于气动阀控技术的微液滴按需喷射装置.该装置主要部件包括储液腔体、位于腔体底部的100μm直径喷嘴、以高速电磁阀和放气管为核心的气路以及液滴拍照系统.该装置利用高速电磁阀产生气体压强脉冲,迫使储液腔内液体挤出喷嘴,形成液滴;气体随后经放气管排出,恢复储液腔内气压平衡.经拍照系统验证,该装置实现了稳定的单液滴喷射,喷射频率可以达到20 Hz,液滴直径约200μm,实现了对人外周血淋巴细胞和培养基的喷射.流式细胞检测显示,细胞经喷射过程的存活率接近100%.

关键词：按需液滴喷射气动细胞打印人外周血淋巴细胞流式细胞术

气动阀控式微米按需液滴喷射过程对细胞活性的影响

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北京工业大学学报 2019年第7期45卷 631-637页

作者：王志海王梦王璨王飞聂志雄陈曦桂晋刚北京工业大学信息学部北京100124 北京工业大学激光工程研究院北京100124 首都医科大学附属北京儿童医院北京儿科研究所北京100035

为了提高气动阀控式微液滴产生装置打印过程中的细胞活性,利用自建气动阀控式微米按需液滴(drop-on-demand,DOD)产生装置,在10~70Hz微液滴产生频率下对2种人体活细胞做喷射实验.2种细胞分别是人外周血单个核细胞(hPBMCs,直径10~15μm)... 详细信息

为了提高气动阀控式微液滴产生装置打印过程中的细胞活性,利用自建气动阀控式微米按需液滴(drop-on-demand,DOD)产生装置,在10~70Hz微液滴产生频率下对2种人体活细胞做喷射实验.2种细胞分别是人外周血单个核细胞(hPBMCs,直径10~15μm)和人支气管上皮细胞(hBECs,直径50~70μm).该喷射装置的喷嘴直径为100μm,微液滴直径180~200μm.实验中,将未经喷射细胞悬液记为对照组,每种频率下向加有磷酸盐缓冲液的样品管中喷射大约6000滴细胞液.随后以7-AAD试剂进行荧光抗体染色,并用流式细胞仪进行细胞活性检测.结果显示,hPBMCs和hBECs喷射后的细胞相对成活率分别为0.991±0.009和0.996±0.014.经分析,喷射频率对细胞活性无显著影响.气动阀控式打印后的细胞活性较高,归因于打印的细胞悬液黏度较低,细胞喷射过程中产生的剪切应力较小.气动阀控微液滴产生技术有望应用在生物细胞打印领域,并为实现体外构建组织器官提供参考.

关键词：气动按需液滴喷射细胞活性喷射频率人外周血单个核细胞人支气管上皮细胞

穿心莲内酯磺化物雾化吸入治疗儿童中重度急性呼吸窘迫综合征疗效探讨

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中国小儿急救医学 2022年第7期29卷 503-509页

作者：方伯梁钱素云桂晋刚国家儿童医学中心首都医科大学附属北京儿童医院重症医学科北京100045 国家儿童医学中心首都医科大学附属北京儿童医院北京市儿科研究所免疫研究室北京100045

目的了解穿心莲内酯磺化物(ADS)雾化吸入对需要有创机械通气的儿童中重度急性呼吸窘迫综合征(ARDS)的治疗效果。方法前瞻性随机对照单盲研究,收集2018年11月1日至2019年12月31日入住首都医科大学附属北京儿童医院PICU,年龄29 d~18岁,且... 详细信息

目的了解穿心莲内酯磺化物(ADS)雾化吸入对需要有创机械通气的儿童中重度急性呼吸窘迫综合征(ARDS)的治疗效果。方法前瞻性随机对照单盲研究,收集2018年11月1日至2019年12月31日入住首都医科大学附属北京儿童医院PICU,年龄29 d~18岁,且需要有创机械通气治疗的中重度ARDS患儿。试验组患儿吸入ADS,对照组吸入生理盐水。试验前后分别留取支气管肺泡灌洗液行细胞因子检测。分析组间人口统计学、细胞因子水平及临床诊疗的差异。结果共纳入患儿20例,男15(75.00%)例,中位年龄2.15(1.48,8.01)岁,中位第2代儿童死亡风险评分为12.25(4.53,16.30)分。两组患儿的人口统计学、基础临床资料及预后比较差异无统计学意义(P>0.05)。试验组患儿的单核细胞趋化蛋白-1降低,对照组上升,差异有统计学意义[967.50(119.25,5206.00)pg/mL比-945.00(-3935.50,495.09)pg/mL,P=0.041]。试验组白细胞介素(IL)-8下降、对照组升高[303.22(-452.00,1172.38)pg/mL比-490.14(-780.25,240.52)pg/mL,P=0.151];试验组IL-6升高幅度低于对照组[-24.53(-501.76,135.27)pg/mL比-325.85(-633.22,133.75)pg/mL,P=0.364];差异均无统计学意义(P>0.05)。试验组患儿研究结束时氧合指数优于对照组[11.35(6.00,15.83)比20.65(6.23,38.35),P=0.374],ARDS好转率较高(80%比60%,P=0.628),但差异均无统计学意义(P>0.05)。两组患儿病死率及机械通气时间差异无统计学意义(P>0.05)。结论ADS雾化吸入或可降低中重度ARDS患儿支气管肺泡灌洗液内IL-6升高幅度,并降低单核细胞趋化蛋白-1、IL-8浓度,且可能改善肺部氧合功能。尚需进一步研究验证上述结论。

关键词：儿童急性呼吸窘迫综合征穿心莲内酯磺化物细胞因子雾化吸入

EBV感染Jurkat T细胞生理生化特性以及转录组分析

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河北医学 2024年第12期30卷 1999-2005页

作者：时瑞柴文佳桂晋刚王微刘祥军首都医科大学附属北京儿童医院肿瘤与免疫研究室北京100037

目的:通过对EBV刺激过表达CD21的Jurkat T细胞进行转录组测序分析,研究EBV感染T细胞的机制。方法:经过PCR技术克隆目的基因CD21,再通过分子克隆技术将CD21基因插入到过表达慢病毒载体上,使其在Jurkat T细胞表面稳定表达。通过EBV刺激过... 详细信息

目的:通过对EBV刺激过表达CD21的Jurkat T细胞进行转录组测序分析,研究EBV感染T细胞的机制。方法:经过PCR技术克隆目的基因CD21,再通过分子克隆技术将CD21基因插入到过表达慢病毒载体上,使其在Jurkat T细胞表面稳定表达。通过EBV刺激过表达CD21的Jurkat T细胞(EBV-LV-Jurkat),然后与未经处理的Jurkat T细胞和仅经过慢病毒感染的Jurkat T细胞(LV-Jurkat)均进行普通转录组测序,并对EBV-LV-Jurkat T与LV-Jurkat T这两组进行差异表达基因的富集通路分析。结果:通过对EBV感染过表达CD21的Jurkat T细胞(EBV-LV-Jurkat T)与过表达CD21的Jurkat T细胞(LV-Jurkat T)差异表达基因(DEGs)GO富集分析发现,其中在分子功能方面主要富集于MAP激酶酪氨酸磷酸酶活性信号通路,其相关基因DUSP1、DUSP10、DUSP5、DUSP8等被显著富集。对EBV-LV-Jurkat T与LV-Jurkat T的DEGs进行KEGG代谢通路分析发现,多种与EBV感染B细胞之后相似的细胞通路以及分子主要富集于MAPK、IL-17、HIF-1、NF-κB与PI3K/AKT等信号通路,以及免疫反应系统相关基因也被显著富集。结论:成功构建了CD21过表达的Jurkat T细胞模型,并通过转录组测序揭示了EBV刺激能够显著上调MAPK信号通路。这为进一步探索EBV感染T细胞的机制提供了重要的初步数据。

关键词： EB病毒基因克隆 CD21基因转录组测序慢病毒载体

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特警队员接触性皮炎的过敏原调查

武警医学 2017年第3期28卷 254-256页

作者：岳红红桂晋刚姜威刘喜花武警总医院过敏科北京100039 北京儿童医院北京市儿科研究所免疫研究室 100045

目的初步了解武警部队特警队员接触性皮炎的发病情况,明确引起接触性皮炎常见的过敏原种类,为接触性皮炎的防治提供依据。方法采用润敏江斑贴试验器,对111名健康特警队员进行斑贴试验,并对试验结果进行统计分析。结果 111位受试健康特... 详细信息

目的初步了解武警部队特警队员接触性皮炎的发病情况,明确引起接触性皮炎常见的过敏原种类,为接触性皮炎的防治提供依据。方法采用润敏江斑贴试验器,对111名健康特警队员进行斑贴试验,并对试验结果进行统计分析。结果 111位受试健康特警队员当中,58例贴斑试验呈阳性反应,阳性率为52.25%。居前6位的常见过敏原依次为卡松、卡巴混合物、硫柳汞、甲醛、芳香混合物和对苯类。1种过敏原阳性反应的有26例,阳性率45%,2种过敏原阳性反应的有20例,阳性率35%,有3种及3种以上过敏原阳性反应的共12例,阳性率21%。在58例阳性患者中,男30例,阳性率为42.25%(30/71);女28例,阳性率为70%(28/40)。女性斑贴试验阳性检出率(70%)明显高于男性(42.25%)。女性对卡松(32.50%,11.27%)、氯化钴(7.50%,0.00%)、硫酸镍(5.00%,0.00%)、松香(12.50%,0.00%)、硫柳汞(25%,7.04%)的阳性检出率明显高于男性。结论特警队员接触性皮炎的过敏原种类与性别有关,斑贴试验有助于明确过敏原。

关键词：接触性皮炎过敏原斑贴试验