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Using MALDI-TOF MS coupled with a high-mass detector to directly analyze intact proteins in thyroid tissues

Science China Chemistry 2018年第7期61卷 871-878页

作者： shan-shan Wang Yun-Jun Wang jing Zhang Jun Xiang Tuan-Qi Sun yin-Long Guo State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in shanghai Center for Excellence in Molecular Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences Department of Head and Neck Surgery Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University

Protein analysis is vital for biological and clinical research, but the measurement of unseparated, intact and high-mass proteins is also a challenging task by mass spectrometry-based methods. Here, we present a protocol for rapid and high-throughput analysis of intact proteins in tissue samples using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDITOF MS） combined with a high-mass detector platform. The method involves tissue specimens that undergo a simple protein extraction before MALDI-MS analysis. Using this method, the high abundance proteins in human thyroid carcinoma and paracarcinoma tissues were successfully investigated, and the mass spectra of the tissues of the 30 illustrated thyroid cancers showed remarkable differences. The peak intensity revealed a significant increase in human albumin in thyroid carcinoma tissues（p＆lt;0.05）. To validate the feasibility and credibility of this method, label-free proteomics quantitative analysis and Western blotting were used to relatively quantify the proteins in these tissues. Those results demonstrated a nearly 3-fold difference in human albumin levels between thyroid carcinoma and para-carcinoma tissues, which were consistent with the results of our method. The advantages of our method are easy sample handling, remarkable reproducibility and the ability to analyze high-mass proteins without digestion, which make them have the potential to be used in biological research and in clinical practice.

关键词： matrix-assisted laser desorption ionization time of flight mass spectrometry intact proteins analysis thyroid carcinoma high-mass detector

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A Generalized Equation of State for High-Pressure Liquids

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Journal of China University of Mining and Technology 2005年第4期15卷 376-378页

作者： LIANG Yan-bo TONG jing-shan Department of Chemistry Xuzhou Normal University Xuzhou Jiangsu 221009 China Department of Chemical Engineering Tsinghua University Beijing 100084 China

An equation of state （EOS） for high-pressure liquids, i.e., Tait EOS, is deduced according to isothermal 1 3V compressibility KT= -1/V· （2V/2p）T·.Based on the equation, a generalized EOS for high pressure-liquids is established by using the reduced state principle and introducing a characteristic parameter-configuration factor ξ. Reasonably satisfactory P-V-T data for many organic compounds, including some polar components, were calculated by using the equation.

关键词： equation of state for high-pressure liquids organic compounds P-V-T data

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Pharmacokinetic characteristics of mesenchymal stem cells in translational challenges

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Signal Transduction and Targeted Therapy 2024年第10期9卷 4314-4340页

作者： Yunlong shan Mengying Zhang Enxiang Tao jing Wang Ning Wei yi Lu Qing Liu Kun Hao Fang Zhou Guangji Wang Key Laboratory of Drug Metabolism and Pharmacokinetics Haihe Laboratory of Cell EcosystemState Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjingChina Jiangsu Renocell Biotech Co.Ltd. NanjingChina

Over the past two decades,mesenchymal stem/stromal cell(MSC)therapy has made substantial strides,transitioning from experimental clinical applications to commercial *** therapies hold considerable promise for treating refractory and critical conditions such as acute graft-versus-host disease,amyotrophic lateral sclerosis,and acute respiratory distress *** recent successes in clinical and commercial applications,MSC therapy still faces challenges when used as a commercial *** detection methods have limitations,leaving the dynamic biodistribution,persistence in injured tissues,and ultimate fate of MSCs in patients *** the relationship between the pharmacokinetic characteristics of MSCs and their therapeutic effects is crucial for patient stratification and the formulation of precise therapeutic ***,the development of advanced imaging and tracking technologies is essential to address these clinical *** review provides a comprehensive analysis of the kinetic properties,key regulatory molecules,different fates,and detection methods relevant to MSCs and discusses concerns in evaluating MSC druggability from the perspective of integrating pharmacokinetics and efficacy.A better understanding of these challenges could improve MSC clinical efficacy and speed up the introduction of MSC therapy products to the market.

关键词： characteristics translational precise

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Effects of Acupuncture at Tianshu(ST25)and shangjuxu(ST37)on the Intestinal Mucosa and Metabolites of Local Skin Tissues at Tianshu(ST25)in 2,4,6-Trinitrobenzene Sulfonic Acid and Ethanol-induced Rats

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World Journal of Traditional Chinese Medicine 2023年第2期9卷 131-140页

作者： jing-ying Zhou yi-Tian Lai Ling Ren Lei Lan Guo-shan Zhang Mi Liu Department of Acupuncture and Moxibustion College of AcupunctureMoxibustion and TuinaHunan University of Chinese MedicineChangshaHunanChina Department of Professional Acupuncture and Moxibustion College of AcupunctureMoxibustion and TuinaHunan University of Chinese MedicineChangshaHunanChina Michael G.DeGroote Institute for Pain Research and Care McMaster UniversityHamiltonOntarioCanada

Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal *** normal rats and four CD modeling rats were selected at random after model identification was ***,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture *** in the acupuncture group underwent acupuncture of Tianshu(ST25)and shangjuxu(ST37)for 7 *** the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)*** local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in ***:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell *** the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly *** choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).

关键词： Acupuncture Crohn's disease metabonomics point Tianshu(ST25)

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Shrinking lung syndrome in autoimmune inflammatory diseases:A case series and review of literature

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World Journal of Emergency Medicine 2022年第3期13卷 254-256页

作者： Xiao-chan Chen shan He jing Xue Department of Rheumatology the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Rheumatology and Immunology the Affiliated Jinhua Hospital of Zhejiang University School of MedicineJinhua 321000China

Dear editor,Shrinking lung syndrome(SLS),a rare complication of autoimmune inflammatory diseases,is mainly associated with systemic lupus erythematosus(SLE),with an occurrence in 1%-2%of cases.^([1])It is characterized by restrictive defects on pulmonary function tests(PFTs)associated with reduced lung *** has also been reported in cases of primary Sjogren syndrome(pSS),^([2,3])scleroderma,^([4])rheumatoid arthritis,^([5])mixed connective tissue disease,⑹and undifferentiated connective tissue ***,we present three case reports of SLS,including two patients with SLE and one patient with adultonset StilFs disease(AOSD)(Table 1).

关键词： lung autoimmune diseases

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Properties of TRPO-HNO_3 complex used for direct dissolution of lanthanide and actinide oxides in supercritical fluid CO_2

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Science China Chemistry 2007年第6期50卷 759-763页

作者： DUAN WuHua ZHU LiYang jing shan ZHU YongJun Institute of Nuclear and New Energy Technology Tsinghua University Beijing 102201 China Institute of Nuclear and New Energy Technology Tsinghua University Beijing 102201 China Institute of Nuclear and New Energy Technology Tsinghua University Beijing 102201 China Institute of Nuclear and New Energy Technology Tsinghua University Beijing 102201 China

The mixed trialkylphosphine oxide-nitric acid (TRPO-HNO3) complex prepared by contacting pure TRPO with concentrated HNO3 may be used as additives for direct dissolution of lanthanide and actinide ox- ides in the supercritical fluid carbon dioxide (SCF-CO2). Properties of the TRPO-HNO3 complex have been studied. Experimental results show when the initial HNO3/TRPO volume ratio is varied from 1:7 to 5:1, the concentration of HNO3 in the TRPO-HNO3 complex changes from 2.12 to 6.16 mol/L, the [HNO3]/[TRPO] ratio of the TRPO-HNO3 complex changes from 0.93 to 3.38, and the content of H2O in the TRPO-HNO3 complex changes from 0.97% to 2.70%. All of the density, viscosity and surface tension of the TRPO-HNO3 complex change with the concentration of HNO3 in the complex. The protons of HNO3 and H2O in the complex undergo rapid exchange to exhibit a singlet resonance peak in NMR spectra with D2O insert. When the TRPO-HNO3 complex dissolves in a low dielectric constant solvent, small droplets of HNO3 appear which can be detected by NMR.

关键词： TRPO-HNO3 complex, property, supercritical fluid carbon dioxide, dissolution

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Quantum Teleportation Through a Two-Qubit Heisenberg XXZ Chain

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Communications in Theoretical Physics 2008年第6期49卷 1435-1438页

作者： GUO Jin-Liang NIE jing SONG He-shan School of Physics and Optoelectronic Technology Dalian University of Technology Dalian 116024 China

We consider a two-qubit Heisenberg XXZ chain as a resource for quantum teleportation via the standard teleportation protocol To. The effects of anisotropic on teleportation fidelity and entanglement are studied in detail. We find anisotropic not only improves the criticM temperature Tc and criticM magnetic field Bc, beyond which quantum teleportation is inferior to classicM communication protocol, but also enhances the fidelity for fixed magnetic field B and temperature T. For entanglement teleportation, the effects of magnetic field on average fidelity and output entanglement are studied.

关键词： thermal entanglement negativity two-qubit Heisenberg XXZ chain

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Modelling the crop yield gap with a remote sensing-based process model:A case study of winter wheat in the North China Plain

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Journal of Integrative Agriculture 2023年第10期22卷 2993-3005页

作者： YANG Xu ZHANG Jia-hua YANG shan-shan WANG jing-wen BAI Yun ZHANG Sha Research Center for Remote Sensing and Digital Earth College of Computer Science and TechnologyQingdao UniversityQingdao 266071P.R.China Aerospace Information Research Institute Chinese Academy of SciencesBeijing 100094P.R.China

Understanding the spatial distribution of the crop yield gap(YG)is essential for improving crop *** studies have typically focused on the site scale,which may lead to considerable uncertainties when scaled to the regional *** mitigate this issue,this study used a process-based and remote sensing driven crop yield model for winter wheat(PRYM-Wheat),which was derived from the boreal ecosystem productivity simulator(BEPS),to simulate the YG of winter wheat in the North China Plain from 2015 to *** validation based on statistical yield data revealed good performance of the PRYM-Wheat Model in simulating winter wheat actual yield(Ya).The distribution of Ya across the North China Plain showed great heterogeneity,decreasing from southeast to *** remote sensing-estimated results show that the average YG of the study area was 6400.6 kg ha^(–1).The YG of Jiangsu Province was the largest,at7307.4 kg ha^(–1),while the YG of Anhui Province was the smallest,at 5842.1 kg ha^(–1).An analysis of the responses of YG to environmental factors showed no obvious correlation between YG and precipitation,but there was a weak negative correlation between YG and accumulated *** addition,the YG was positively correlated with *** general,studying the specific features of the YG can provide directions for increasing crop yields in the future.

关键词： remote sensing PRYM-Wheat Model yield gap environmental factors North China Plain

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A Study on Service-Oriented Smart Medical Systems Combined with Key Algorithms in the IoT Environment

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China Communications 2019年第9期16卷 235-249页

作者： shan Lu Anzhi Wang Shenqi jing Tao shan Xin Zhang Yongan Guo Yun Liu Department of Geriatrics the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Department of Health Education the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Engineering Research Center of Health Service System Based on Ubiquitous Wireless Networks Ministry of EducationNanjing University of Posts and TelecommunicationsNanjing 210003China

A smart medical service system architecture is proposed in this paper to increase medical resource utilization and improve the efficiency of the medical diagnosis process for complex business scenarios in the Medical Internet of Things(MIoT)*** resource representation model theory,multi-terminal aggregation algorithm,and the resource discovery algorithm based on latent factor model are also studied.A smart medical service system within the IoT environment is then developed,based on the open source *** results using real-world datasets illustrate that the proposed smart medical service system architecture can promote the intelligent and efficient management of medical resources to an extent,and assists in the develop towards digitization,intelligence,and precision in the field of medicine.

关键词： Internet of Things resource representationmodel resource discovery algorithm smart medical service system

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Nonreciprocal mechanical entanglement in a spinning optomechanical system

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Chinese Physics B 2024年第11期33卷 200-206页

作者： shan-shan Chen jing-jing Zhang Jia-Neng Li Na-Na Zhang Yong-Rui Guo Huan Yang School of Optoelectronic Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China

Quantum entanglement between distant massive mechanical oscillators is an important resource in sensitive measurements and quantum information *** achieve the nonreciprocal mechanical entanglement in a compound optomechanical device consisting of two mechanical oscillators and a spinning whispering-gallery mode(WGM)optical *** is found that obvious nonreciprocal mechanical entanglement emerges in this system in the presence of the Sagnac effect which is induced by the rotation of the WGM resonator,and the nonreciprocal region can be controlled by tuning the angular velocity of the *** nonreciprocity originates from the breaking of the time-reversal symmetry of this multimode system due to the presence of the Sagnac *** optomechanical coupling and the mechanical interaction provide cooling channels for the first and second mechanical oscillators,*** mechanical oscillators can be cooled *** simultaneous cooling and the mechanical coupling of two mechanical oscillators ensure the generation of mechanical ***,an optimal mechanical entanglement can be achieved when the moderate optical frequency detuning and the driving power are *** thermal noise of the mechanical environment has a negative effect on mechanical *** scheme provides promising opportunities for research of quantum information processing based on phonons and sensitive measurements.

关键词： optomechanical system quantum entanglement nonreciprocity Sagnac effect

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