Effects of Acupuncture at Tianshu(ST25)and Shangjuxu(ST37)on the Intestinal Mucosa and Metabolites of Local Skin Tissues at Tianshu(ST25)in 2,4,6-Trinitrobenzene Sulfonic Acid and Ethanol-induced Rats

作     者：Jing-Ying Zhou Yi-Tian Lai Ling Ren Lei Lan Guo-Shan Zhang Mi Liu 

作者机构：Department of Acupuncture and MoxibustionCollege of AcupunctureMoxibustion and TuinaHunan University of Chinese MedicineChangshaHunanChina Department of Professional Acupuncture and MoxibustionCollege of AcupunctureMoxibustion and TuinaHunan University of Chinese MedicineChangshaHunanChina Michael G.DeGroote Institute for Pain Research and CareMcMaster UniversityHamiltonOntarioCanada 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2023年第9卷第2期

页      面：131-140页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：National Natural Science Foundation of China(No.81603705,No.81774438) China Postdoctoral Science Foundation Project(No.2107M612567) Natural Science Foundation of Hunan Province(No.2021JJ30513,No.2017JJ3245) Scientific Research Project of Hunan Provincial Department of Education(No.21A0235,No.20B444) Hunan Traditional Chinese Medicine Research Project(No.C2022027) Hunan Province Youth Backbone Teacher Training Target Project(2021) Changsha Natural Science Foundation(No.kq2208183) 

主　　题：Acupuncture Crohn's disease metabonomics point Tianshu(ST25) 

摘      要：Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn s disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal *** normal rats and four CD modeling rats were selected at random after model identification was ***,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture *** in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 *** the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)*** local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in ***:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell *** the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly *** choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P0.05 or P0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P0.05 or P0.01).