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The Use of Zein and Shuanghuangbu for Periodontal Tissue Engineering

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International Journal of Oral Science 2010年第3期2卷 142-148页

作者： yan-zhi Xu jing-jing Wu yan-ping Chen Jian Liu Na Li Feng-ying yang Department of Stomatology the Fourth Hospital of Hebei Medical University Shijiazhuang China

Aim Tissue engineering is a promising area with a broad range of applications in the fields of regenerative medicine and human health. The emergence of periodontal tissue engineering for clinical treatment of periodontal disease has opened a new therapeutic avenue. The choice of scaffold is crucial. This study was conducted to prepare zein scaffold and explore the suitability of zein and Shuanghuangbu for periodontal tissue *** A zein scaffold was made using the solvent casting/particulate leaching method with sodium chloride （NaC1） particles as the porogen. The physical properties of the zein scaffold were evaluated by observing its shape and determining its pore structure and porosity. Cytotoxicity testing of the scaffold was carried out via in vitro cell culture experiments, including a liquid extraction experi- ment and the direct contact assay. Also, the Chinese medicine Shuanghuangbu, as a growth factor, was diluted by scaffold extract into different concentrations. This Shuanghuangbu-scaffold extract was then added to periodontal ligament cells （PDLCs） in order to determineits effect on cell proliferation. Results The zein scaffold displayed a sponge-like structure with a high porosity and sufficient thickness. The porosity and pore size of the zein scaffold can be controlled by changing the porogen particles dosage and size. The porosity was up to 64.1%-78.0%. The pores were well-distributed, interconnected, and porous. The toxicity of the zein scaffold was graded as 0-1. Furthermore, PDLCs displayed full stretching and vigorous growth under scanning electronic microscope （SEM）. Shuanghuangbu-scaffold extract could reinforce proliferation activity of PDLCs compared to the control group, especially at 100 μ***^-1 （P〈0.01）. Conclusion A zein scaffold with high porosity, open pore wall structure, and good biocompatibility is conducive to the growth of PDLCs. Zein could be used as scaffold to repair periodontal tissue defects. Also, Shuanghu

关键词： zein periodontal tissue engineering scaffold,periodontal ligament cells Shuanghuangbu

Prognostic value of T cell immunoglobulin and mucin-domain containing-3 expression in upper gastrointestinal tract tumors:A meta-analysis

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World Journal of Gastrointestinal Oncology 2023年第12期15卷 2212-2224页

作者： jing-jing yan Bing-Bing Liu yan yang Meng-Ru Liu Han Wang Zhen-Quan Deng Zhi-Wei Zhang College of Clinical Medicine Hebei University of EngineeringHandan 056000Hebei ProvinceChina Department of Oncology Handan First HospitalHandan 056002Hebei ProvinceChina Department of Oncology Affiliated Hospital of Hebei University of EngineeringHandan 056000Hebei ProvinceChina

BACKGROUND There is a lack of robust prognostic markers for upper gastrointestinal(GI)tract cancers,including esophageal,gastric,and esophagogastric junction cancers.T cell immunoglobulin and mucin-domain containing-3(TIM3)plays a key immunomodulatory role and is linked to the prognosis of various ***,the significance of TIM3 in upper GI tract tumors is still *** To investigate the prognostic value of TIM3 expression in upper GI tract *** A literature search was conducted on the PubMed,Embase,and Web of Science databases for relevant studies published until June *** screening and quality assessment,studies that met the criteria were included in the *** methods were used for the pooled analysis to assess the association of TIM3 expression in upper GI tract tumors with the prognosis and clinicopathological *** results were reported with the hazard ratio(HR)and 95%confidence interval(CI).RESULTS Nine studies involving 2556 patients with upper GI tract cancer were *** TIM3 expression was associated with a worse prognosis in upper GI tract cancer(HR:1.17,95%CI:1.01-1.36).Positive expression of TIM3 in gastric cancer was correlated with the T and N stage,but the difference was not statistically ***,TIM3 overexpression was significantly correlated with the TNM stage(odds ratio:1.21,95%CI:0.63-2.33;P<0.05).TIM3 expression showed no association with the other clinicopathological *** High expression of TIM3 in the upper GI tract cancer is associated with a worse prognosis and advanced T or N stages,indicating its potential value as a prognostic *** findings may provide a basis for the personalized treatment of upper GI tract cancers.

关键词： Immune checkpoint T cell immunoglobulin-3 Upper gastrointestinal tract cancer Overall survival Clinicopathological features

Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第9期11卷 719-727页

作者： Wei WANG Li WANG Xin-xiu LI Xia CHEN Hai-yan ZHANG Yu HE jing-jing WANG Yong-yan ZHAO Bao-le ZHANG Yin-xue XU College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China

Bone morphogenetic proteins(BMPs) play a critical role in the growth and steroidogenesis of granulosa cells(GCs).BMP signals act through membrane-bound heteromeric serine/threonine kinase *** ligand binding,BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell *** objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs.A strategy of RNA interference(RNAi)-mediated 'gene silencing' of Smad4,a core molecule mediating the intracellular BMP/Smad signal transduction pathways,was used to interrupt endogenous BMP/Smad *** indicate that Smad4-small interfering RNA(siRNA) caused specific inhibition of Smad4 mRNA and protein expression after *** endogenous BMP/Smad signaling significantly inhibited growth,and induced apoptosis of porcine GCs,while decreasing estradiol *** addition,interrupted BMP/Smad signaling significantly(P<0.05) changed the expression of Cyclin D2,CDK4,Bcl-2,and *** findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.

关键词： Porcine granulosa cells Bone morphogenetic protein(BMP)/Smad Smad4 RNA interference(RNAi) Growth Steroidogenesis

Clinical features of acute hepatitis E super-infections on chronic hepatitis B

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World Journal of Gastroenterology 2016年第47期22卷 10388-10397页

作者： Chong Chen Shu-Ye Zhang Dan-Dan Zhang Xin-yan Li Yu-Ling Zhang Wei-Xia Li jing-jing yan Min Wang jing-Na Xun Chuan Lu Yun Ling Yu-Xian Huang Liang Chen Wenzhou Medical University Wenzhou 325000 Zhejiang Province China Shanghai Public Health Clinical Center Fudan University Shanghai 201508 China Institutes of Biomedical Sciences Fudan UniversityShanghai 200433 China School of Life Sciences Hebei Normal University Shijiazhuang 050000 Hebei Province China

AIM To examine the clinical features and risk factors for adverse outcomes in chronic hepatitis B(CHB) superimposed with hepatitis E virus(HEV).METHODS This retrospective cohort study included 228 patients with acute HEV infection(showing clinical acute hepatitis symptomology and positivity for anti-HEV immunoglobulin M) with underlying CHB(confirmed by positivity for hepatitis B surface antigen and/or hepatitis B virus(HBV) DNA over 6 mo) who had been admitted to the Shanghai Public Health Clinical Center, which represents the regional tertiary hospital for infectious diseases in Shanghai city, China. Data for adverse outcomes were collected, and included severe liver diseases(defined as liver failure and/or acute liver decompensation) and liver-related mortality. Logistic regression modeling was performed to determine the risk factors for adverse *** The symptoms caused by superimposed acute hepatitis E(AHE) were much more severe in cirrhotic patients(n = 94) than in non-cirrhotic patients(n = 134), as evidenced by significantly higher liver complications(77.7% vs 28.4%, P < 0.001) and mortality rate(21.3% vs 7.5%, P = 0.002). Most of the cirrhotic patients(n = 85, 90.4%) had no prior decompensation. Among the non-cirrhotic patients, superimposed AHE caused progressively more severe diseases that corresponded with the CHB disease stages, from immune tolerant to immune reactivation phases. Few risk factors were identified in the cirrhotic patients, but risk factors for non-cirrhotic patients were found to be intermediate HBV DNA levels(OR: 5.1, P = 0.012), alcohol consumption(OR: 6.4, P = 0.020), and underlying diabetes(OR: 7.5, P = 0.003) and kidney diseases(OR: 12.7, P = 0.005). Only 28.7% of the cirrhotic patients and 9.0% of the non-cirrhotic patients had received anti-HBV therapy previously and, in all cases, the efficacy had been suboptimal. CONCLUSION CHB-related cirrhosis and intermediate HBV DNA level were associated with severe disease in sup

关键词： Cirrhosis Co-infections Liver failure Liver decompensation Stages of hepatitis B virus infection

Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion

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World Journal of Gastroenterology 2008年第1期14卷 29-37页

作者： Wei-Xing Chen Fang Wang, Yu-Ying Liu, Qing-Jiang Zeng, Kai Sun, Xin Xue, Xiang Li, Ji-Ying yang, Li-Hua An,Bai-He Hu, Jin-Hui yang, Chuan-She Wang, Zhi-Xin Li, Lian-Yi Liu, yan Li, Jun Zheng, Fu-Long Liao, Dong Han,jing-Yu Fan, jing-yan Han Fang Wang Yu-Ying Liu Qing-Jiang Zeng Kai Sun Xin Xue Xiang Li Ji-Ying yang Li-Hua An Bai-He Hu Jin-Hui yang Chuan-She Wang Zhi-Xin Li Lian-Yi Liu yan Li Jun Zheng Fu-Long Liao Dong Han jing-Yu Fan jing-yan Han Tasly Microcirculation Research Center Peking University Health Science Center Beijing 100083 China Department of Pharmacology Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Department of Integrated Chinese and Western Medicine School of Basic Medical Sciences Peking University Beijing 100083 China

AIM： To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion （I/R） in mice. METHODS： The superior mesenteric artery （SMA） of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-rain reperfusion. In another set of experiments, R1 was continuously infused （10 mg/kg per hour） from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell （RBC） velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase （LDH）, alanine aminotransferase （ALl＇） and aspartate transaminase （AST） in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor- alpha （TNF-α）, interleukin-6 （IL-6） and monocyte chemotactic protein-1 （MCP-1） in plasma were evaluated by flow Oltometry. E-selectin and intercellular adhesion molecule-1 （ICAM-1） in hepatic tissue were examined by ***： After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1. CONCLUSION： R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury, The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils.

关键词： Ischemia/reperfusion Notoginsenoside R1 Leukoytes adhesion E-selectin Hepatic injury

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong jing-jing Hu yan Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu Xiao-Jia yang Qian-Ru He Fei Ding Hai-yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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不同储藏温湿度对玉米中蛋白质的影响

食品工业科技 2017年第3期38卷 331-335页

作者：殷晶晶赵妍田晓花河南工业大学粮油食品学院河南郑州450001 山东商务职业学院山东烟台264670

以"郑单958"品种玉米为对象,模拟中国蒙新、华北、华中和华南储粮微环境条件(15℃,50%RH;20℃,65%RH;28℃,75%RH和35℃,85%RH),以研究储藏期间玉米蛋白质的变化规律,结果表明储藏时间对玉米的总蛋白含量几乎无影响(p>0.05);随着储藏时... 详细信息

以"郑单958"品种玉米为对象,模拟中国蒙新、华北、华中和华南储粮微环境条件(15℃,50%RH;20℃,65%RH;28℃,75%RH和35℃,85%RH),以研究储藏期间玉米蛋白质的变化规律,结果表明储藏时间对玉米的总蛋白含量几乎无影响(p>0.05);随着储藏时间延长,玉米中清蛋白、球蛋白、醇溶蛋白和巯基含量呈下降趋势(p<0.05),而谷蛋白和二硫键含量则呈现上升趋势;储藏微环境对蛋白组分以及巯基和二硫键含量的变化影响显著(p<0.05)。与低温低湿条件(15℃,50%RH)相比,高温高湿条件(35℃,85%RH)下,玉米中清蛋白、球蛋白、醇溶蛋白和巯基含量分别减少了0.24、0.13、0.26 g/100 g和1.19μmol/g,谷蛋白和二硫键含量分别增加了0.74 g/100 g和2.05μmol/g。因此,低温低湿环境(15℃,50%RH)更利于玉米安全储藏。

关键词：玉米储藏微环境蛋白质组分巯基二硫键

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-yan Sun Lingyun Nie jing Zhang Xue Fang Hongmei Luo Chuanhai Fu Zhiyi Wei Ai-Hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

Inflammatory responses of the rat lungs in cold-dryness syndrome in the northwest of China

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Journal of Traditional Chinese Medicine 2012年第2期32卷 234-237页

作者：高振哈木拉提·吾甫尔荆晶廖春燕徐丹李风森 Traditional Chinese Medicine Hospital affiliated with Xinjiang Medical University & National Clinical Research Base of Traditional Chinese Medicine Xin Jiang Medical University

OBJECTIVE:To examine changes in body weight and the lung inflammation factors interleukin-1β(IL-1β),interleukin-8(IL-8),IL-10 and tumor necrosis factor-α(TNF-α) in a rat model of cold-dryness syndrome in Northwest(Xinjiang) China to provide a reference for treating chronic obstructive pulmonary disease(COPD) with local ***:The rat COPD model was established by intratracheal instillation of porcine pancreatic elastase(PPE) in combination with cigarette smoking(CS).The rat model of cold-dryness syndrome of COPD in the northwest of China was set up by intratracheal instillation of PPE in combination with CS and environmental cold-dryness *** level of IL-1β,IL-8,IL-10 and TNF-α in Bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay(ELISA).The data were analyzed using the software SPSS ***:1) Body weight was less in the two model groups than that of control group(P<0.01),PPE plus CS cold-dryness group was less than that of PPE plus CS group(P<0.01).2) IL-1β in BALF significantly increased in PPE plus CS and cold-dryness group than that of control group(P<0.01).3) IL-8 and TNF-α in BALF significantly increased in PPE plus CS and cold-dryness group and PPE plus CS group than that of control group(P<0.01).CONCLUSION:Body weight in COPD model rats was reduced compared with ***-dryness may aggravate such a condition Lung inflammation in the model was mainly manifested by an increase in IL-1β,IL-8 and TNF-α levels,with no change in IL-10 ***-dryness may aggravate lung inflammation of COPD.

关键词： Pulmonary disease Chronic obstructive pulmonary disease Cold-dryness syndrome Northwest China Inflammatory reaction