检索结果-南通市图书馆

Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

引用

World Journal of Stem Cells 2024年第3期16卷 305-323页

作者： Jian-jing lin Tong Ning Shi-Cheng Jia Ke-Jia Li Yong-Can Huang Qiang Liu Jian-Hao lin Xin-Tao Zhang Department of Sports Medicine and Rehabilitation Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Institute of Medical Science The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250033Shandong ProvinceChina Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijing 100871China Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China

BACKGROUND Mesenchymal stem cells(MSCs)modulated by various exogenous signals have been applied extensively in regenerative medicine ***,nanosecond pulsed electric fields(nsPEFs),characterized by short duration and high strength,significantly influence cell phenotypes and regulate MSCs differentiation via multiple ***,we used transcriptomics to study changes in messenger RNA(mRNA),long noncoding RNA(lncRNA),microRNA(miRNA),and circular RNA expression during nsPEFs *** To explore gene expression profiles and potential transcriptional regulatory mechanisms in MSCs pretreated with *** The impact of nsPEFs on the MSCs transcriptome was investigated through whole transcriptome *** were pretreated with 5-pulse nsPEFs(100 ns at 10 kV/cm,1 Hz),followed by total RNA *** transcript was normalized by fragments per kilobase per *** change and difference significance were applied to screen the differentially expressed genes(DEGs).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate gene functions,complemented by quantitative polymerase chain reaction *** In total,263 DEGs were discovered,with 92 upregulated and 171 *** were predominantly enriched in epithelial cell proliferation,osteoblast differentiation,mesenchymal cell differentiation,nuclear division,and wound *** cellular components,DEGs are primarily involved in condensed chromosome,chromosomal region,actin cytoskeleton,and *** aspect of molecular functions,DEGs are mainly involved in glycosaminoglycan binding,integrin binding,nuclear steroid receptor activity,cytoskeletal motor activity,and steroid *** real-time polymerase chain reaction confirmed targeted transcript *** Our systematic investigation of the wide-ranging transcriptional pattern modulated by nsPEFs revealed the differential expression of 26

关键词： Nanosecond pulsed electric fields Whole transcriptome sequencing Mesenchymal stem cells Genetic response Stem cell engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Temperature-and Strain-Compensated Directional Curvature Sensor Based on Bragg Gratings in Three-Core Fiber with Bridged Waveguides

引用

中国激光 2024年第18期 1-10页

作者： Wu Zhifang Wei Yefen lin jing Wang Hupo Huang Ruimin Wang Xiaoyan Pu Jixiong Fujian Key Laboratory of Light Propagation and Transformation College of Information Science and Engineering Huaqiao University

Fiber-based curvature sensors, especially those capable of discerning the direction of curvature, have attracted more and more interest due to their promising applications in structural health monitoring, high-precision measurement, medical and biological diagnosis-treat instruments, and so on. Here, we propose and demonstrate a compact directional curvature sensor that comprises two bridged waveguides and three Bragg gratings in a section of three-core fiber（TCF）. Both the waveguides and gratings are integrated by femtosecond laser micromachining method. The waveguides, connecting the TCF outer cores to the lead-in single-mode fiber core, function as beam couplers to realize simultaneous interrogation of all three gratings without any separate fan-in/out component. Owing to the spatial specificity, the outer-core gratings exhibit high and direction-dependent sensitivity to curvature, whereas the central-core grating is nearly insensitive to curvature but shows similar sensitivities to ambient temperature and axial strain as the outer-core gratings. It can be used to compensate the cross impact of temperature and strain when the outer-core gratings are applied for curvature detection. Moreover, the wavelength interval between two outer-core gratings is also proposed as an indicator for curvature sensing. It features with a much higher sensitivity to curvature and reduced sensitivities to temperature and axial strain. The corresponding maximum sensitivity to curvature is as high as 191.89 pm/m-1, while the sensitivities to temperature and strain are only 0.3 pm/℃ and 0.0218 pm/με, respectively. Therefore, our proposed device provides a compact and robust all-infiber solution for directional curvature sensing. It not only offers high sensitivity and accuracy but also immunity to temperature and axial strain fluctuations, making it a promising tool for a wide range of applications.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

STAT3-Dependent Effects of Polymeric Immunoglobulin Receptor in Regulating Interleukin-17 Signaling and Preventing Autoimmune Hepatitis

引用

Engineering 2024年第5期36卷 209-222页

作者： Ting Li Tongtong Pan Nannan Zheng Xiong Ma Xiaodong Wang Fang Yan Huimian Jiang Yuxin Wang Hongwei lin jing lin Huadong Zhang Jia Huang lingming Kong Anmin Huang Qingxiu Liu Yongping Chen Dazhi Chen Hepatology Diagnosis and Treatment Center The First Affiliated Hospital of Wenzhou Medical University&Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver DiseasesWenzhou 325035China Department of Clinical Medicine Hangzhou Medical CollegeHangzhou 310053China State Key Laboratory for Oncogenes and Related Genes Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Wenzhou Medical University Wenzhou 325035China Division of Infectious Diseases Lishui People’s HospitalLishui 323000China

One-third of patients with autoimmune hepatitis(AIH)have cirrhosis at the time of *** relevance of these variables,although unknown,is believed to be critical in AIH because of suspected interactions between the gut microbiome and genetic *** of the gut flora and elevated polymeric immunoglobulin receptor(pIgR)levels have been observed in both patients and mouse ***,there is a direct relationship between pIgR expression and transaminase levels in patients with *** this study,we aimed to explore how pIgR influences the secretion of regenerating islet-derived 3 beta(Reg3b)and the flora composition in AIH using in vivo experiments involving patients with AIH and a concanavalin A-induced mouse model of ***3b expression was reduced in pIgR gene(Pigr)-knockout mice compared to that in wild-type mice,leading to increased microbiota ***,exogenous pIgR supplementation increased Reg3b expression and maintained microbiota *** sequencing revealed the participation of the interleukin(IL)-17 signaling pathway in the regulation of Reg3b through ***,the introduction of external pIgR could not restore the imbalance in gut microbiota in AIH,and the decrease in Reg3b expression was not apparent following the inhibition of signal transducer and activator of transcription 3(STAT3).In this study,pIgR facilitated the upregulation of Reg3b via the STAT3 pathway,which plays a crucial role in preserving the balance of the intestinal microbiota in *** this research,we discovered new molecular targets that can be used for the diagnosis and treatment of AIH.

关键词： Autoimmune hepatitis Polymeric immunoglobulin receptor Regenerating islet-derived 3 beta Intestinal microbiota Signal transducer and activator of transcription 3

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Personification in Chinese Diplomatic Discourse and the Construction of National Identity Based on Corpus:A Case Study of the Regular Press Conference of Ministry of Foreign Affairs

引用

Journal of Literature and Art Studies 2024年第5期14卷 360-364页

作者： XIONG jing-lin ZHOU jing Wuhan University of Technology WuhanChina

With the development of the major country diplomacy with Chinese characteristics,diplomatic discourse,as an important part of building China’s international discourse power,has attracted much *** is a common means in diplomatic *** giving anthropomorphic expressions to countries,regions,institutions and policies,the purpose of building national identity and establishing national image can be *** on conceptual metaphor theory and national identity theory,this paper focuses on personification in diplomatic discourse with a case study of the regular press conference of Ministry of Foreign *** is found that the spokesperson skillfully constructed China’s positive national identity,such as peace-loving,openness and inclusiveness by using a large number of body metaphor,kinship metaphor and role metaphor.

关键词： diplomatic discourse personification identity construction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aflibercept combined with triamcinolone acetonide in the treatment of diabetic macular edema:optical coherence tomography and optical coherence tomography angiography

引用

International Journal of Ophthalmology(English edition) 2024年第4期17卷 670-675页

作者： De-Shuang Li Hong-Xia Liao Chuan-He Zhang Jian-Guo Huang Wei Chen jing-lin Zhang Bo Qin Shenzhen Aier Eye Hospital Aier Eye HospitalJinan UniversityShenzhen 518031Guangdong ProvinceChina Department of Ophthalmology the University of Hong Kong-Shenzhen HospitalShenzhen 518000Guangdong ProvinceChina Guangzhou Aier Eye Hospital Jinan UniversityGuangzhou 510040Guangdong ProvinceChina

AIM:To analyze the relationship between optical coherence tomography(OCT)and OCT angiography(OCTA)imaging in patients with diabetic macular edema(DME)who are treated with a combination of aflibercept and triamcinolone acetonide(TA).METHODS:A total of 76 eyes newly diagnosed DME were included in this *** were randomly assigned to receive either aflibercept or a combination of aflibercept and *** once a month for a total of three *** macular thickness(CMT),number of hyperreflective foci(HRF),height of subretinal fluid(SRF),and area of foveal avascular zone(FAZ)were evaluated using OCT and OCTA at baseline and after each monthly ***:Both groups showed improvement in best corrected visual acuity(BCVA)and reduction in macular edema after treatment,and the difference in BCVA between the two groups was statistically significant after each treatment(P<0.05).The difference in CMT between the two groups was statistically significant after the first two injections(P<0.01),but not after the third injection(P=0.875).The number of HRF(1mo:7.41±8.25 vs 10.86±7.22,P=0.027;2mo:5.33±6.13 vs 9.12±8.61,P=0.034;3mo:3.58±3.00 vs 6.37±5.97,P=0.007)and height of SRF(1mo:82.39±39.12 vs 105.77±42.26μm,P=0.011;2mo:36.84±10.02 vs 83.59±37.78μm,P<0.01;3mo:11.57±3.29 vs 45.43±12.60μm,P<0.01)in combined group were statistically significant less than aflibercept group after each injection,while the area of FAZ showed no significant change before and after treatment in both ***:The combination therapy of aflibercept and TA shows more significant effects on DME eyes with decreased HRF and ***,both aflibercept and combination therapy show no significant change in the area of FAZ.

关键词： diabetic macular edema optical coherence tomography optical coherence tomography angiography hyperreflective foci subretinal fluid foveal avascular zone aflibercept

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acute liver failure:A clinically severe syndrome characterized by intricate mechanisms

引用

World Journal of Hepatology 2024年第7期16卷 1067-1069页

作者： Ran An jing-lin Wang Division of Hepatobiliary and Transplantation Surgery Department of General SurgeryNanjing Drum Tower HospitalNanjing 210008Jiangsu ProvinceChina

Acute liver failure presents as a clinical syndrome characterized by swift deterioration and significant mortality *** underlying mechanisms are intricate,involving intricate interplays between various *** the current scarcity of treatment options,there's a pressing need to diligently uncover the disease's core mechanisms and administer targeted therapies accordingly.

关键词： Acute liver failure Hepatocyte Macrophage Necroptosis Pyroptosis Ferroptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

First Trimester Preeclampsia Screening and Prevention:Perspective in Chinese Mainland

引用

Maternal-Fetal Medicine 2024年第2期6卷 84-91页

作者： Jiao Liu Yunyu Chen Sin Ting Tai Long Nguyen-Hoang Kunping Li jing lin Xiaohong Lu Liona C.Poon Department of Obstetrics and Gynecology The Chinese University of Hong KongShatinHong Kong 999077China

Preeclampsia(PE),a multisystem disorder in pregnancy,is one of the leading causes of perinatal morbidity and mortality that poses financial and physical burdens *** PE with delivery at<37 weeks of gestation is associa... 详细信息

关键词： Preeclampsia Chinese population First trimester Screening Prevention

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

L-theanine/α-Ketoglutarate/Nrf2 axis promotes exercise-mediated amelioration of NASH in mice

引用

Food Science and Human Wellness 2024年

作者： Wang-jing Mu Min Chen Jie-Ying Zhu Hong-Yang Luo Yang Li Shan Li lin-jing Yan Ruo-ying Li Liang Guo School of Exercise and Health and Collaborative Innovation Center for Sports and Public Health， Shanghai University of Sport Shanghai Frontiers Science Research Base of Exercise and Metabolic Health， Shanghai University of Sport Key Laboratory of Exercise and Health Sciences of the Ministry of Education， Shanghai University of Sport

L-Theanine (LTA) is a non-protein amino acid mainly found in tea plants with many beneficial effects. Exercise exerts a wide range of benefits in metabolic health. Here， we show that exercise or gastric lavage intervention on mice with LTA improves diet-induced NASH in mice. Meanwhile， combinatory therapy shows that exercise and LTA synergistically improve obesity-related metabolic disorders and NASH phenotypes， including hepatic steatosis， inflammation， cell death and oxidative stress. In vivo studies indicate that LTA inhibits free fatty acid (FFA)-induced hepatocyte injury， including steatosis， oxidative stress and apoptosis. Knockdown of Nrf2 blunts the role of LTA in inhibiting FFA-induced hepatocyte oxidative stress and dysfunction. Mechanistically， LTA increases the α-ketoglutarate (α-KG) level in hepatocytes， which increases the transcription of Nrf2 by inducing active DNA demethylation on its promoter. Moreover， LTA promote the above α-KG/Nrf2 axis in synergy with exercise， thereby more efficiently inhibiting hepatic oxidative stress and ameliorating diet-induced NASH in mice. Our results suggest that， through promoting the α-KG/Nrf2 axis-mediated anti-oxidative pathway， the combination of LTA and exercise may provide an effective measure for the prevention and control of NASH.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Id2 epigenetically controls CD8^(+)T-cell exhaustion by disrupting the assembly of the Tcf3-LSD1 complex

引用

Cellular & Molecular Immunology 2024年第3期21卷 292-308页

作者： Yiming Li Mingwei Han Haolin Wei Wan Huang Zhinan Chen Tianjiao Zhang Meirui Qian lin jing Gang Nan Xiuxuan Sun Shuhui Dai Kun Wang Jianli Jiang Ping Zhu Liang Chen Department of Cell Biology of National Translational Science Center for Molecular Medicine and Department of Clinical Immunology of Xijing Hospital Fourth Military Medical UniversityXi’anShaanxi 710032China State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases GanzhouJiangxi341000Xi’anShaanxi 710032China School of Medicine Shanghai UniversityShanghai 200444China

CD8^(+)T-cell exhaustion is a state of dysfunction that promotes tumor progression and is marked by the generation of Slamf6^(+)progenitor exhausted(Tex^(prog))and Tim-^(3+)terminally exhausted(Tex^(term))*** of DNA binding protein 2(Id2)has been shown to play important roles in T-cell development and CD8^(+)T-cell ***,the role of Id2 in CD8^(+)T-cell exhaustion is ***,we found that Id2 transcriptionally and epigenetically regulates the generation of Texprog cells and their conversion to Texterm *** deletion of Id2 dampens CD8^(+)T-cell-mediated immune responses and the maintenance of stem-like CD8^(+)T-cell subpopulations,suppresses PD-1 blockade and increases tumor ***,through its HLH domain,Id2 binds and disrupts the assembly of the Tcf3-Tal1 transcriptional regulatory complex,and thus modulates chromatin accessibility at the Slamf6 promoter by preventing the interaction of Tcf3 with the histone lysine demethylase ***,Id2 increases the abundance of the permissive H3K4me2 mark on the Tcf3-occupied E-boxes in the Slamf6 promoter,modulates chromatin accessibility at the Slamf6 promoter and epigenetically regulates the generation of Slamf6+Texprog *** LSD1 inhibitor GSK2879552 can rescue the Id2 knockout phenotype in tumor-bearing *** of LSD1 increases the abundance of Slamf6^(+)Tim-3^(−)Tex^(prog) cells in tumors and the expression level of Tcf1 in Id2-deleted CD8+T *** study demonstrates that Id2-mediated transcriptional and epigenetic modification drives hierarchical CD8^(+)T-cell exhaustion,and the mechanistic insights gained may have implications for therapeutic intervention with tumor immune evasion.

关键词： Id2 T-cell exhaustion Epigenetic modification Immune evasion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

High-Power Raman Soliton Generation at 1.7 μm in All-Fiber Polarization-Maintaining Erbium-Doped Amplifier

引用

Chinese Physics Letters 2024年第5期41卷 35-39页

作者：徐子鹏王萱姚传飞杨林京李平雪 Institute of Ultrashort Pulsed Laser and Application Beijing University of TechnologyBeijing 100124China

An all-fiber polarization maintaining high-power laser system operating at 1.7 μm based on the Ramaninduced soliton self-frequency shifting effect is demonstrated. The entirely fiberized system is built by erbiumdoped oscillator and two-stage amplifiers with polarization maintaining commercial silica fibers and devices, which can provide robust and stable soliton generation. High-power soliton laser with the average power of 0.28 W,the repetition rate of 42.7 MHz, and pulse duration of 515 fs is generated directly from the main *** experiment provides a feasible method for high-power all-fiber polarization maintaining femtosecond laser generation working at 1.7 μm.

关键词： polarization fiber amplifier

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：