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High-density Linkage Map of Cultivated Allotetraploid Cotton Based on SSR, TRAP, SRAP and AFLP Markers

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Journal of Integrative Plant Biology 2007年第5期49卷 716-724页

作者： Jiwen Yu Shuxun Yu Cairui Lu Wu Wang Shuli Fan Meizhen Song Zhongxu Lin Xianlong zhang jinfa zhang National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China Key Laboratory for Cotton Genetic Improvement Ministry of Agriculture Anyang 455004 China China Cotton Research Institute Chinese Academy of Agricultural Science Anyang 455004 China Department of Plant and Environmental Sciences New Mexico State University Las Cruces NM 88003 USA

A high-density linkage map was constructed for an F2 population derived from an Interspecific cross of cultivated allotetraploid species between Gossypium hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the Interspecific cross of ＂CRI 36 × Hal 7124＂ were genotyped at I 252 polymorphic loci Including a novel marker system, target region amplification polymorphism （TRAP）. The map consists of 1 097 markers, including 697 simple se- quence repeats （SSRs）, 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were Identified In tetraploid cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton.

关键词： cotton linkage map target region amplification polymorphism

A comparative analysis of small RNAs between two Upland cotton backcross inbred lines with different fiber length: Expression and distribution

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The Crop Journal 2019年第2期7卷 198-208页

作者： Guoyuan Liu Man Wu Wenfeng Pei Xihua Li Nuohan Wang Jianjiang Ma Xinshan Zang Shuxun Yu jinfa zhang Jiwen Yu State Key Laboratory of Cotton Biology Cotton Institute of the Chinese Academy of Agricultural Sciences Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture Department of Plant and Environmental Sciences New Mexico State University

The cotton fiber is the most important raw material for the textile industry and an ideal model system for studying cell elongation. However, the genetic variation of fiber elongation in relation to miRNA is poorly understood. A high-throughput comparative RNA-seq of two lines differing in fiber length(FL) from a backcross inbred line(BIL)population of G. hirsutum × G. barbadense revealed differentially expressed(DE) miRNAs and their targets in rapidly elongating fibers. A real-time quantitative PCR analysis was further performed to validate the results. A total of 463(including 47 DE) miRNAs were identified,and seven DE miRNAs were co-localized with seven FL quantitative trait loci(QTL)identified in the G. hirsutum × G. barbadense population. Of 82(including 21 DE) targets identified, nine(including one DE) were also co-localized with the seven FL QTL. The relationship between the allopolyploid and its diploid ancestral species with respect to miRNAs and their targets was also characterized. These results will facilitate the understanding of the molecular genetic mechanism of fiber elongation with regards to miRNAs in cotton.

关键词： Allopolyploid Gossypium Fiber elongation miRNA QTL hotspots

A targeted QTL analysis for fiber length using a genetic population between two introgressed backcrossed inbred lines in upland cotton(Gossypium hirsutum)

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The Crop Journal 2019年第3期7卷 273-282页

作者： Guoyuan Liua Wenfeng Pei Dan Li Jianjiang Ma Yupeng Cui Nuohan Wang Jikun Song Man Wu Libei Li Xinshan Zang Shuxun Yu jinfa zhang Jiwen Yu State Key Laboratory of Cotton Biology Cotton Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Cotton Genetic ImprovementMinistry of AgricultureAnyang 455000HenanChina Xinjiang Research Base State Key Laboratory of Cotton BiologyXinjiang Agricultural UniversityUrumqi 830001XinjiangChina Department of Plant and Environmental Sciences New Mexico State UniversityLas CrucesNM 88003USA

Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in *** trait locus(QTL)mapping based on high-density genetic maps is an efficient approach to identify genetic regions for *** our study, two backcrossed inbred lines(BILs) were chosen as parents to construct a high-density genetic map in F2 which was used to fine map FL QTL in F2:3 *** genetic map had a total size of 3462.8 cM, containing 9182 singlenucleotide polymorphisms(SNPs) based on *** FL related stable QTL were identified on two chromosomes(qFL-A08–1 on A08 and qFL-D03–1 on D03),and qFL-A08–1 was confirmed by a *** previously obtained RNA-seq data for the two BILs and qRT-PCR analysis, two candidate genes annotated as cytochrome b5(CB5, Gh_A08 G1729) and microtubule end-binding 1 C(EB1C, Gh_D03 G0232) that may regulate FL during the fiber elongation stage were *** addition, nine recombination hotspots in this population were *** results of this study will provide an important foundation for further studies on the molecular and genetic regulation of fiber elongation.

关键词： Gossypium Single-nucleotide polymorphism Fiber length Quantitative trait locus

利用马克隆值差异显著的BILs鉴定棉纤维品质相关基因

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棉花学报 2020年第1期32卷 52-62页

作者：吴嫚李龙云裴文锋 zhang jinfa 于霁雯中国农业科学院棉花研究所/棉花生物学国家重点实验室郑州大学研究基地河南安阳455000 Department of Plant and Environmental Science New Mexico State UniversityLas CrucesNM88003USA

【目的】马克隆值是衡量棉纤维品质的重要指标之一。利用马克隆值存在显著差异的2个陆海回交近交系材料进行高通量基因芯片分析,旨在筛选和鉴定棉纤维发育相关的关键基因。【方法】从SG747(Gossypium hirsutum L.)和Giza75(Gossypium Ba... 详细信息

【目的】马克隆值是衡量棉纤维品质的重要指标之一。利用马克隆值存在显著差异的2个陆海回交近交系材料进行高通量基因芯片分析,旨在筛选和鉴定棉纤维发育相关的关键基因。【方法】从SG747(Gossypium hirsutum L.)和Giza75(Gossypium Barbadense L.)高世代回交近交系(Backcross Inbred Lines,BILs)群体中选取NMGA-140(马克隆值6.2)和NMGA-051(马克隆值4.0)为材料,利用Affymetrix公司的棉花寡聚核苷酸基因芯片对其开花后10 DPA(Days after anthesis)的棉纤维进行了表达谱分析。【结果】获得了2655个差异表达基因,包括功能预测基因(15.57%),翻译、核糖体结构与生物合成相关基因(13.54%)和翻译后修饰、蛋白质转换、分子伴侣相关基因(9.31%)等。推测β-tub10正向调控棉纤维发育过程,β-tub1负向调控棉纤维发育过程,Ghi.3578.1.S1_s_at在棉纤维发育早期起到了作用。【结论】为棉花纤维品质的遗传改良提供了丰富的基因资源,为分子标记辅助育种开发更多新的分子标记。

关键词：棉纤维品质马克隆值基因芯片差异表达基因

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磁集成开关变换器的小信号建模方法

电工技术学报 2015年第24期30卷 103-109页

作者：卢增艺陈为章进法台达电子企业管理(上海)有限公司上海201209 福州大学电气工程与自动化学院福州350108

全面认识和客观评价磁集成技术对开关变换器稳态和动态性能的影响,需要建立磁集成开关变换器的小信号模型。论文以集成磁件分析方法为基础,结合状态空间平均法,分别提出三种磁集成开关变换器小信号建模方法,为这类变换器的动态特性研究... 详细信息

全面认识和客观评价磁集成技术对开关变换器稳态和动态性能的影响,需要建立磁集成开关变换器的小信号模型。论文以集成磁件分析方法为基础,结合状态空间平均法,分别提出三种磁集成开关变换器小信号建模方法,为这类变换器的动态特性研究和控制设计建立理论基础。以磁集成有源钳位正激变换器为研究对象,分别对这三种建模方法做详细阐述。

关键词：磁集成小信号建模状态空间正激变换器

Genome-wide association study of micronaire using a natural population of representative upland cotton(Gossypium hirsutum L.)

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Journal of Cotton Research 2021年第2期4卷 114-125页

作者： SONG Jikun PEI Wenfeng MA Jianjiang YANG Shuxian JIA Bing BIAN Yingying XIN Yue WU Luyao ZANG Xinshan QU Yanying zhang jinfa WU Man YU Jiwen State Key Laboratory of Cotton Biology Ministry of Agriculture and Rural Affairs/Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang455000HenanChina Engineering Research Centre of Cotton Ministry of Education/College of AgricultureXinjiang Agricultural UniversityUrumqi830052XinjiangChina Department of Plant and Environmental Sciences New Mexico State UniversityLas Cruces880033NMUSA

Background: Micronaire is a comprehensive index reflecting the fineness and maturity of cotton *** is one of the important internal quality indicators of the cotton fiber and is closely related to the value of the cotton *** the genetic basis of micronaire is required for the genetic improvement of the ***,the genetic architecture of micronaire at the genomic level is *** present genome-wide association study(GWAS)aimed to identify the genetic mechanism of the micronaire trait in 83 representa:tive upland cotton lines grown in multiple *** GWAS of micronaire used 83 upland cotton accessions assayed by a Cotton 63 K Illumina Infinium single nucleotide polymorphism(SNP)array.A total of 11 quantitative trait loci(QTLs)for micronaire were detected on 10 *** 11 QTLs included 27 identified genes with specific expression patterns.A novel QTL,qFM-A12–1,included 12 significant SNPs,and GhFLA9 was identified as a candidate gene based on haplotype block analysis and on strong and direct linkage disequilibrium between the significantly related SNPs and ***9 was expressed at a high level during secondary wall thickening at 20∼25 days *** expression level of GhFLA9 was significantly higher in the low micronaire line(Msco-12)than that in the high micronaire line(Chuangyou-9).Conclusions: This study provides a genetic reference for genetic improvement of cotton fiber micronaire and a foundation for verification of the functions of GhFLA9.

关键词： Upland cotton(Gossypium hirsutum L.) Fiber micronaire GWAS Candidate genes GhFLA9

DETERMINATION OF ELECTRON TEMPERATURE IN PLASMA VIA DEIONIZED PERIOD MEASUREMENT BY OPTOCAL DELAY METHOD

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Journal of Electronics(China) 1988年第1期5卷 16-19页

作者：甄汉生吴锦发张二力 Department of Radio & Electronics Qinghua University

For the determination of electron temperature in plasma,this paper presents a method tomeasure the deionized period by optical attenuation during *** apparatus was a tube filled to 1mmHg helium with a probe for *** results agreed with the values measured by the *** tousing plasma radiation,one need not worry that the discharge space would be disturbed and the probe wouldbe contaminated by depositing discharge products,So it is a simple and convenient method.

关键词： Gas discharge plasma Electron tempeatue Deionzed time

棉花陆海回交自交系群体叶绿素含量性状QTL定位

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棉花学报 2020年第5期32卷 463-471页

作者：耿延会边盈盈裴文锋刘国元吴嫚臧新山李丹李兴丽 zhang jinfa 于霁雯棉花生物学国家重点试验室/中国农业科学院棉花研究所/农业部棉花生物学与遗传育种重点试验室河南安阳455000 新墨西哥州立大学美国新墨西哥州拉斯克鲁塞斯88003

【目的】对棉花叶片叶绿素含量数量性状位点(Quantitative trait loci, QTL)进行定位。【方法】以中36为轮回亲本与海7124配制的195个BC1F7家系为研究材料。根据本课题已构建的高密度遗传图谱,利用QTL IciMapping 4.1中的BIP(QTL mappin... 详细信息

【目的】对棉花叶片叶绿素含量数量性状位点(Quantitative trait loci, QTL)进行定位。【方法】以中36为轮回亲本与海7124配制的195个BC1F7家系为研究材料。根据本课题已构建的高密度遗传图谱,利用QTL IciMapping 4.1中的BIP(QTL mapping in bi-parental populations)和MET(QTL mapping for multi-enviromental trials)模型采用完备区间作图法(Inclusive composite interval mapping, ICIM)分别对初花期和结铃期基于Soil and Plant Analyzer Development(SPAD)读值的叶绿素含量进行QTL分析。【结果】检测到9个与叶绿素含量相关的QTLs分布在6条染色体上。其中,与初花期叶绿素含量显著相关的q-SPAD-A11-1与结铃期检测到的q-SPAD-A11-2具有较大重叠,表型贡献率分别为5.08%和5.75%;q-SPAD-D08-2位于D08染色体的48.71~53.65 Mb与前人定位结果相近。【结论】本研究首次鉴定的与叶绿素含量性状稳定相关q-SPAD-A11为叶绿素含量性状进一步的精细定位奠定了基础。

关键词：数量性状叶绿素含量棉花 QTL定位

腹腔镜胆囊切除术与开腹胆囊切除术治疗老年急性胆囊炎的疗效比较

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国际医药卫生导报 2016年第17期22卷 2628-2630页

作者：张进法叶县人民医院普外科平顶山467200

目的探讨与比较腹腔镜胆囊切除术与开腹胆囊切除术治疗老年急性胆囊炎的临床疗效。方法收集本院2013年8月至2015年8月收治的急性胆囊炎老年患者100例前瞻性研究且随机分为两组，每组50例，腹腔镜组患者给予腹腔镜胆囊切除术治疗，开腹... 详细信息

目的探讨与比较腹腔镜胆囊切除术与开腹胆囊切除术治疗老年急性胆囊炎的临床疗效。方法收集本院2013年8月至2015年8月收治的急性胆囊炎老年患者100例前瞻性研究且随机分为两组，每组50例，腹腔镜组患者给予腹腔镜胆囊切除术治疗，开腹组则给予开腹胆囊切除术治疗，比较两组患者的手术相关指标、术后相关临床指标与并发症情况。结果腹腔镜组患者切口长度、术中出血量与手术时间显著低于开腹组[（2．19±0．57）cm vs．（8．78±1．87）cm、（58．47±20．14）ml vs．（95．49±34．47）ml、（59．34±13．75）min vs．（78．28±18．67）min]，两组比较差异有统计学意义（P〈0．01）；腹腔镜组患者肠道恢复时间、拆线时间与住院时间显著低于开腹组[（3．07±1．11）d vs．（5．87±1．85）d、（7．05±1．35）d vs．（14．35±2．72）d、（7．98±1．46）d vs．（12．76±2．25）d]，两组比较差异有统计学意义（P〈0．01）；腹腔镜组患者总体并发症发生率显著低于开腹组（6．0％vs．30．0％），两组比较差异有统计学意义（P〈0．01）。结论腹腔镜胆囊切除术治疗老年急性胆囊炎术中创伤小、术后恢复快、安全性高，总体临床效果优于开腹胆囊切除术，具有借鉴性。

关键词：腹腔镜胆囊切除术开腹胆囊切除术急性胆囊炎并发症