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Regulation of biotic interactions and responses to abiotic stresses by MAP kinase pathways in plant pathogenic fungi

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Stress Biology 2021年第1期1卷 46-64页

作者： xue Zhang Zeyi Wang Cong Jiang jin-rong xu State Key Laboratory of Crop Stress Biology for Arid Areas and NWAFU-Purdue Joint Research Center College of Plant ProtectionNorthwest A&F UniversityYangling 712100ShaanxiChina Department of Botany and Plant Pathology Purdue UniversityWest LafayetteIN 47907USA

Like other eukaryotes,fungi use MAP kinase(MAPK)pathways to mediate cellular changes responding to external *** the past two decades,three well-conserved MAP kinase pathways have been characterized in various plant pathogenic fungi for regulating responses and adaptations to a variety of biotic and abiotic stresses encountered during plant infection or survival in *** invasive growth(IG)pathway is homologous to the yeast pheromone response and filamentation *** plant pathogens,the IG pathway often is essential for pathogenesis by regulating infection-related morphogenesis,such as appressorium formation,penetration,and invasive *** cell wall integrity(CWI)pathway also is important for plant infection although the infection processes it regulates vary among fungal *** its universal function in cell wall integrity,it often plays a minor role in responses to oxidative and cell wall *** the IG and CWI pathways are involved in regulating known virulence factors as well as effector genes during plant infection and mediating defenses against mycoviruses,bacteria,and other *** contrast,the high osmolarity growth(HOG)pathway is dispensable for virulence in some fungi although it is essential for plant infection in *** regulates osmoregulation in hyphae and is dispensable for appressorium turgor *** HOG pathway also plays a major role for responding to oxidative,heat,and other environmental stresses and is overstimulated by phenylpyrrole ***,these three MAPK pathways crosstalk and coordinately regulate responses to various biotic and abiotic *** IG and CWI pathways,particularly the latter,also are involved in responding to abiotic stresses to various degrees in different fungal pathogens,and the HOG pathway also plays a role in interactions with other microbes or ***,some infection processes or stress responses are co-regulated by MAPK pathways with cAMP or Ca^(2+)

关键词： Fungal pathogens Signal transduction Pathogenesis Virulence Oxidative stress Osmotic stress Fungicide resistance Bacterial-fungal interactions

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Deletion of all three MAP kinase genes results in severe defects in stress responses and pathogenesis in Fusarium graminearum

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Stress Biology 2022年第1期2卷 621-633页

作者： jingyi Ren Yuhan Zhang Yuhua Wang Chengliang Li Zhuyun Bian xue Zhang Huiquan Liu jin-rong xu Cong Jiang State Key Laboratory of Crop Stress Biology for Arid Areas and NWAFU-Purdue Joint Research Center College of Plant ProtectionNorthwest A&F UniversityYangling 712100ShaanxiChina Department of Botany and Plant Pathology Purdue UniversityWest LafayetteIN 47907USA

Mitogen-activated protein kinase(MAPK)cascades are activated by external stimuli and convert signals to cellular *** MAPKs have been characterized in a number of plant pathogenic fungi for their roles in pathogenesis and responses to biotic or abiotic ***,mutants deleted of all the MAPK genes have not been reported in filamentous *** determine the MAPK-less effects in a fungal pathogen,in this study we generated and characterized mutants deleted of all three MAPK genes in the wheat scab fungus Fusarium *** Gpmk1 mgv1 Fghog1 triple mutants had severe growth defects and was *** was defective in infection cushion formation and DON *** was reduced in the triple mutant,which often produced elongated conidia with more septa than the wild-type *** triple mutant was blocked in sexual reproduction due to the loss of female *** of any MAPKs resulted in an increased sensitivity to various abiotic stress including cell wall,osmotic,oxidative stresses,and phytoalexins,which are likely related to the defects of the triple mutant in environmental adaptation and plant *** triple mutant also had increased sensitivity to the biocontrol bacterium Bacillus velezensis and fungus Clonostachys *** co-incubation assays with ***,the Gpmk1 mgv1 Fghog1 mutant had more severe growth limitation than the wild type and was defective in conidium germination and germ tube *** confrontation assays,the triple mutant was defective in defending against mycoparasitic activities of *** and the latter could grow over the mutant but not wild-type ***-seq and metabolomics analyses showed that the MAPK triple mutant was altered in the expression of many ATP-binding cassette(ABC)and major facilitator superfamily(MFS)transporter genes and the accumulation of metabolites related to arachidonic acid,linoleic acid,and alpha-linolenic acid ***,as the first

关键词： Abiotic stresses Fungal-bacterial interaction Gibberella zeae Mycoparasitism Signal transduction

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Herbicide 2,4-dichlorophenoxyacetic acid interferes with MAP kinase signaling in Fusarium graminearum and is inhibitory to fungal growth and pathogenesis

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Stress Biology 2023年第1期3卷 345-361页

作者： Kaili Duan Qifang Shen Yu Wang Ping Xiang Yutong Shi Chenfei Yang Cong Jiang Guanghui Wang jin-rong xu xue Zhang State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYangling 712100ShaanxiChina Department of Botany and Plant Pathology Purdue UniversityWest LafayetteIN 47907USA

Plant hormones are important for regulating growth,development,and plant-pathogen *** of them are inhibitory to growth of fungal pathogens but the underlying mechanism is not *** this study,we found that hyphal growth of Fusarium graminearum was significantly reduced by high concentrations of IAA and its metabolically stable analogue 2,4-dichlorophenoxyacetic acid(2,4-D).Besides inhibitory effects on growth rate,treatments with 2,4-D also caused significant reduction in conidiation,conidium germination,and germ tube *** with 2,4-D had no obvious effect on sexual reproduction but significantly reduced TRI gene expression,toxisome formation,and DON *** importantly,treatments with 2,4-D were inhibitory to infection structure formation and pathogenesis at concentrations higher than 100μ*** presence of 1000μM 2,4-D almost completely inhibited plant infection and invasive *** ***,2,4-D induced ROS accumulation and FgHog1 activation but reduced the phosphorylation level of Gpmk1 MAP *** analysis showed that the accumulation of a number of metabolites such as glycerol and arabitol was increased by 2,4-D treatment in the wild type but not in the Fghog1 *** expressing the dominant active FgPBS2^(S451D T455D) allele were less sensitive to 2,4-D and had elevated levels of intracellular glycerol and arabitol induced by 2,4-D in *** together,our results showed that treatments with 2,4-D interfere with two important MAP kinase pathways and are inhibitory to hyphal growth,DON biosynthesis,and plant infection in ***.

关键词： Fungal pathogenesis MAP kinase pathways 2,4-D,Fusarium graminearum

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Genome-wide A-to-I RNA editing during sexual reproduction in filamentous ascomycetes

Genome-wide A-to-I RNA editing during sexual reproduction in...

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中国菌物学会2016年学术年会

作者： Huiquan Liu Yang Li jin-rong xu Purdue-NWAFU Joint Research Center State Key Laboratory of Crop Stress Biology for Arid AreasCollege of Plant ProtectionNorthwest A&F University Dept.of Botany and Plant Pathology Purdue University

＜正＞Yeasts and filamentous fungi do not have Adenosine Deaminase Acting on RNA（ADAR）orthologs and are believed to lack A-to-I RNA editing,which is the most prevalent m RNA editing m RNA in ***,we recently found that A-to-I editing occurs specifically during sexual reproduction in the wheat scab fungus Fusarium ***-wide analysis identified 26,056 perithecium-specific A-to-I editing *** those in animals,

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Evaluation of hepatitis B viral replication and proteomic analysis of HepG2.2.15 cell line after knockdown of HBx

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Hepatobiliary & Pancreatic Diseases International 2011年第3期10卷 295-302页

作者： Hai-Yang Xie,Jun Cheng,Chun-Yang Xing,jin-jin Wang,rong Su,xu-Yong Wei,Lin Zhou and Shu-Sen Zheng Author Affiliations:Key Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health Key Laboratory of Organ Transplantation Zhejiang Province and Division of Hepatobiliary and Pancreatic Surgery,Department of Surgery,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health Key Laboratory of Organ Transplantation Zhejiang Province and Division of Hepatobiliary and Pancreatic Surgery Department of Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Hepatitis B virus (HBV) is one of the major pathogens of human liver *** have shown that HBV X protein (HBx) plays an important role in promoting viral gene expression and *** this study we performed a global proteomic profiling to identify the downstream functional proteins of HBx,thereby detecting the mechanisms of action of HBx on virion ***:HBx in the HepG2.2.15 cell line was knocked down by the transfection of small interfering RNA (siRNA).The replication level of HBV was evaluated by microparticle enzyme immunoassay analysis of HBsAg and HBeAg in the culture supernatant,and real-time quantitative PCR analysis of HBV ***-dimensional electrophoresis combined with MALDI-TOF/TOF was performed to analyze the changes in protein expression profile after treatment with HBx ***:Knockdown of HBx disturbed HBV replication in *** target siRNA significantly inhibited the expression of HBsAg,HBeAg and the replication of HBV *** significantly changed proteins (7 upregulated and 5 downregulated) were successfully identified by MALDI-TOF/TOF using proteomics differential expression analysis after the knockdown of *** these identified proteins,HSP70 was validated by Western ***:The results of the study indicated the positive effect of HBx on HBV replication,and a group of downstream target proteins of HBx may be responsible for this effect.

关键词： hepatitis B virus HBx replication proteomics

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Complete response of gallbladder cancer treated with gemcitabine and cisplatin chemotherapy combined with durvalumab:A case report and review of literature

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 226-232页

作者： Kai Chen xu Feng Yuan Shi Xin-Lin Li Zheng-rong Shi Xiang Lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Gallbladder cancer(GBC)is the most common and aggressive subtype of biliary tract cancer(BTC)and has a poor prognosis.A newly developed regimen of gemcitabine,cisplatin,and durvalumab shows promise for the treatment of advanced ***,the efficacy of this treatment for GBC remains *** SUMMARY In this report,we present a case in which the triple-drug regimen exhibited marked effectiveness in treating locally advanced GBC,thus leading to a long-term survival benefit.A 68-year-old man was diagnosed with locally advanced GBC,which rendered him ineligible for curative *** three cycles of therapy,a partial response was *** one year of combined therapy,a clinical complete response was successfully *** maintenance therapy with durvalumab monotherapy resulted in a disease-free survival of 9 months for the *** patient experienced tolerable toxicities of reversible grade 2 nausea and *** adverse events were observed in the patient throughout the entirety of the *** The combination of gemcitabine and cisplatin chemotherapy with durvalumab was proven to be an effective treatment approach for advanced GBC,with manageable adverse *** research is warranted to substantiate the effectiveness of the combined regimen in the context of GBC.

关键词： Gallbladder cancer Chemotherapy Immunotherapy Durvalumab Complete response Case report

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HCV NS4基因在大肠杆菌中表达及蛋白质提纯

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武汉大学学报（理学版） 2001年第4期47卷 468-470页

作者：王晓玲叶林柏郜金荣徐进平吴正辉武汉大学病毒研究所湖北武汉430072

应用 PCR方法扩增出人丙型肝炎病毒全长 N S4基因的 DNA片段 ,克隆入表达载体 p QE3 2并转化*** JM10 9,经 IPTG诱导表达出 4 2× 10 3蛋白 .利用 Ni- NTA- agarose纯化得到纯化产物 ,Western- blot鉴定该蛋白能够与抗 NS4的单克隆抗体... 详细信息

应用 PCR方法扩增出人丙型肝炎病毒全长 N S4基因的 DNA片段 ,克隆入表达载体 p QE3 2并转化*** JM10 9,经 IPTG诱导表达出 4 2× 10 3蛋白 .利用 Ni- NTA- agarose纯化得到纯化产物 ,Western- blot鉴定该蛋白能够与抗 NS4的单克隆抗体发生特异性反应 .

关键词：丙型肝炎病毒 NS4蛋白纯化 NS4基因大肠杆菌基因表达生物功能

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丙型肝炎病毒NS5B基因在昆虫细胞中的表达

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武汉大学学报（自然科学版） 2001年第2期47卷 217-219页

作者：李磊郜金荣徐进平叶林柏张斌吴正辉武汉大学病毒研究所湖北武汉430072

将含有丙型肝炎病毒 (HCV) NS5 B基因的转移载体 p Blue Bac5 B与野生型杆状病毒 (Ac NPV) DNA共转染 Sf9昆虫细胞 ,通过空斑纯化获得带有 NS5 B基因的重组病毒 Ac NS5 B.提取重组病毒基因组 DNA进行Southern分析 ,发现有一条 1.8kb的 ... 详细信息

将含有丙型肝炎病毒 (HCV) NS5 B基因的转移载体 p Blue Bac5 B与野生型杆状病毒 (Ac NPV) DNA共转染 Sf9昆虫细胞 ,通过空斑纯化获得带有 NS5 B基因的重组病毒 Ac NS5 B.提取重组病毒基因组 DNA进行Southern分析 ,发现有一条 1.8kb的 DNA片段与标记的探针发生杂交 .Ac NS5 B感染 Sf9细胞后 ,在细胞中表达了分子量为 6 6× 10 3的蛋白 ,用 Western blot方法检查这种蛋白质 ,能与兔抗 HCVNS5

关键词：丙型肝炎病毒 NS5B基因重组杆状病毒昆虫细胞基因表达 RNA多聚酶

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基于可调Q因子小波变换的芯片图像去噪研究

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仪表技术与传感器 2021年第12期 106-110页

作者：樊博田瑞金旭荣郭林明国网宁夏营销服务中心(计量中心) 宁夏银川750011 四川大学电气工程学院四川成都610065

噪声会降低芯片图像的字符识别准确率,为了去除芯片图像的噪声干扰,并保留芯片图像的字符细节,提出了基于参数优化的可调Q因子小波变换(TQWT)的芯片图像去噪方法。首先根据图片的弱纹理区域,使用极大似然估计来估计噪声强度,进而优化TQW... 详细信息

噪声会降低芯片图像的字符识别准确率,为了去除芯片图像的噪声干扰,并保留芯片图像的字符细节,提出了基于参数优化的可调Q因子小波变换(TQWT)的芯片图像去噪方法。首先根据图片的弱纹理区域,使用极大似然估计来估计噪声强度,进而优化TQWT的参数。然后,根据最优参数,使用TQWT对含噪图像沿水平和竖直方向分解,剔除高频噪声子带,使用剩余子带进行重构去噪。实验表明,优化参数后的TQWT能将噪声完全分离,可以在去噪的同时保留字符的细节信息,在视觉效果、峰值信噪比和结构相似性上都优于对比算法。

关键词：可调Q因子小波变换噪声估计图像去噪芯片图像

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Application of ultrasound- assisted headspace liquid phase microextraction in the determination of volatile halohydrocarbons in real water samples by gas chromatography

Application of ultrasound- assisted headspace liquid phase m...

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中国化学会第十四届有机分析及生物分析学术研讨会

作者： YAO jin-rong CHENG jing xu Hui~* (Key Laboratory of Pesticide & Chemical Biology,Ministry of Education,College of Chemistry,Central China Normal University,Wuhan 430079,People’s Republic of China)

Volatile halohydrocarbons (VHHs) usually can be determined in drinking *** potential hazard has aroused public attention to the determination of *** the present study,a novel analytical method for extraction of three VHHs (CHCl,CHCl and CCl) from real water samples has been described by combining of ultrasound-assist- ed headepace liquid-phase microextraction (UAHS-LPME) with gas chromatography microelectron capture detection (C,C-μECD).At the base of the conventional HS-LPME,this new technique has been improved in some aspects:a) A micro-insert (2.00 μL) was used to suspend the extractant microdrop instead of the microsyringe,b) Ultrasonic vi- bration was used for extraction instead of the magnetic stirrer,c) Ice bath was used to obtain the relatively low temper- ature of *** the assistant of ultrasonic extraction,three VHHs are headspace extracted into the organic solvent,which suspended on the bottom of a *** extraction,the organic solvent loaded with analytes is injected into *** order to obtain the best extraction efficiency,a series of parameters are investigated and optimized *** the optimal condition,4 μL hexamethylene was used as the extractant to extract the analytes,the initial sample temperature was 30℃,10 mL sample solution was imported into 15 mL vial,after ultrasonic extraction for 20 min,the analytes were injected into GC for *** new technique has been applied to determine three VHHs (CH Cl,CHCl and CCl) in East River and tap *** presented protocol is compared with conventional HS-LPME,the extraction efficiency of this new method is 4.4,6.9,3.7 times higher than that of conventional meth- od for CHCl,CHCl and CCl,respectively,and the enrichment factor is 100,72 and *** linear calibration craves in the concentration range of 1.00～30.00 μg/L,0.20～40.00 μg/L and 0.05～5.00 μg/L were obtained for the three *** limits of detection (LOD) ranged fiom 0.007 to 0.490 μg/L are obt

关键词： Ultrasound-assisted headspace liquid-phase microextraction Gas chromatography Volatile halohydrocar-bons

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