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Improved Isolation and Culture of Embryonic Germ Cells from Guanzhong Dairy Goat

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Agricultural Sciences in China 2006年第7期5卷 550-557页

作者： YANG Wei-feng GE Xiu-guo HUA jin-lian SHEN Wen-zheng DOU Zhong-ying Shaanxi Center of Stem Cells Engineering ＆ Technology Northwest A ＆ F University Yangling 712100 P.R.China

A total of 219 embryonic-germ-cell-like （EG-like） clumps were derived from 15 selected goat fetuses. Isolation of primordial germ cells （PGCs） based on co-culture with primary goat embryonic fibroblast showed no difference from traditional feeder layer-based culture method used in mouse and human. The putative primary EG colonies were multilayer clumps of compact cells with unclear cell-cell boundaries. Three subculture methods of goat EG-like colony, traditional enzymatic digestion, mechanical cutting and combination of the both, were compared in this study. As a result, EG-like colonies traditionally disassociated with collagenase 1V could be subcultured for up to 4 passages. And the mechanically disaggregated EG-like colonies were successfully maintained 9-12 passages with or without enzymatic treatment. The pluripotency of the EG-like colonies was identified by their specific marker staining, spontaneous differentiation and embryoid bodies （EBs） formation in vitro. Most goat EG-like colonies （〉 80%） were AKP positive and immunocytochemically characterized with positive SSEA-1, Oct-4 and c-kit staining but SSEA-4. Under the condition of delaying passage, goat EG-like cells could differentiate into fibroblast-like, epithelium-like, and neuron-like cells. In addition, EBs could be obtained successfully in routine hanging drop culture. The serum free culture system （feeder layer-based） used in this study was suitable for keeping PGCs and EG-like cells in their undifferentiated condition, but failed to converse them to immortal cells. These results indicated that mechanical cutting is an effective method for passaging goat EG cell colonies. However, the microenvironment of conversing EG cells to immortal cells is still unclear.

关键词： goat primordial germ cells embryonic germ cells co-culture mechanical cutting

Identification of reservoir types in deep carbonates based on mixedkernel machine learning using geophysical logging data

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Petroleum Science 2024年第3期21卷 1632-1648页

作者： jin-Xiong Shi Xiang-Yuan Zhao lian-Bo Zeng Yun-Zhao Zhang zheng-Ping Zhu Shao-Qun Dong Key Laboratory of Exploration Technologies for Oil and Gas Resources of the Ministry of Education Yangtze UniversityWuhan4310HubeiChina School of Geosciences Yangtze UniversityWuhan430100HubeiChina College of Geosciences China University of Petroleum(Beijing)Beijing102249China Petroleum Exploration and Production Research Institute of SINOPEC Beijing100083China School of Earth Sciences and Engineering Xi'an Shiyou UniversityXi'an 710065ShaanxiChina

Identification of reservoir types in deep carbonates has always been a great challenge due to complex logging responses caused by the heterogeneous scale and distribution of storage *** cross-plot analysis and empirical formula methods for identifying reservoir types using geophysical logging data have high uncertainty and low efficiency,which cannot accurately reflect the nonlinear relationship between reservoir types and logging ***,the kernel Fisher discriminant analysis(KFD),a kernel-based machine learning technique,attracts attention in many fields because of its strong nonlinear processing ***,the overall performance of KFD model may be limited as a single kernel function cannot simultaneously extrapolate and interpolate well,especially for highly complex data *** address this issue,in this study,a mixed kernel Fisher discriminant analysis(MKFD)model was established and applied to identify reservoir types of the deep Sinian carbonates in central Sichuan Basin,*** MKFD model was trained and tested with 453 datasets from 7 coring wells,utilizing GR,CAL,DEN,AC,CNL and RT logs as input *** particle swarm optimization(PSO)was adopted for hyper-parameter optimization of MKFD *** evaluate the model performance,prediction results of MKFD were compared with those of basic-kernel based KFD,RF and SVM ***,the built MKFD model was applied in a blind well test,and a variable importance analysis was *** comparison and blind test results demonstrated that MKFD outperformed traditional KFD,RF and SVM in the identification of reservoir types,which provided higher accuracy and stronger *** MKFD can therefore be a reliable method for identifying reservoir types of deep carbonates.

关键词： Reservoir type identification Geophysical logging data Kernel Fisher discriminantanalysis Mixedkernel function Deep carbonates

Diving dynamics identification and motion prediction for marine crafts using field data

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Journal of Ocean Engineering and Science 2024年第4期9卷 391-400页

作者： Yiming Zhong Caoyang Yu Yulin Bai zheng Zeng lian lian School of Oceanography Shanghai Jiao Tong UniversityShanghai 200030China Key Laboratory of Polar Ecosystem and Climate Change Ministry of EducationShanghai Jiao Tong UniversityShanghai 200030China Shanghai Key Laboratory of Polar Life and Environment Sciences Shanghai Jiao Tong UniversityShanghai 200030China State Key Laboratory of Ocean Engineering Shanghai Jiao Tong UniversityShanghai 200240China

Ensuring accurate parameter identification and diving motion prediction of marine crafts is essential for safe navigation,optimized operational efficiency,and the advancement of marine *** this,this paper proposes an instrumental variable-based least squares(IVLS)***,aiming to balance complexity with accuracy,a decoupled diving model is constructed,incorporating nonlinear actuator characteristics,inertia coefficients,and damping ***,a discrete parameter identification matrix is designed based on this dedicated model,and then a IVLS algorithm is innovatively derived to reject measurement ***,the stability of the proposed algorithm is validated from a probabilistic point of view,providing a solid theoretical ***,performance evaluation is conducted using four depth control datasets obtained from a piston-driven profiling float in Qiandao Lake,with desired depths of 30 m,40 m,50 m,and 60 *** on the diving dynamics identification results,the IVLS algorithm consistently shows superior performance when compared to recursive weighted least squares algorithm and least squares support vector machine algorithm across all depths,as evidenced by lower average absolute error(AVGAE),root mean square error(RMSE),and maximum absolute error values and higher determination coefficient(R2).Specifically,for desired depth of 60 m,the IVLS algorithm achieved an AVGAE of 0.553 m and RMSE of 0.655 m,significantly outperforming LSSVM with AVGAE and RMSE values of 8.782 m and 11.117 m,***,the IVLS algorithm demonstrates a remarkable generalization capability with R2 values consistently above 0.95,indicating its robustness in handling varied diving dynamics.

关键词： Marine craft Parameter identification Motion prediction Instrumental variable-based least-squares algorithm Diving dynamics model

Classification of musical hallucinations and the characters along with neural-molecular mechanisms of musical hallucinations associated with psychiatric disorders

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World Journal of Psychiatry 2024年第9期14卷 1386-1396页

作者： Xin lian Wei Song Tian-Mei Si Naomi zheng lian Department of Radiology and Biomedical Imaging Shanxi Bethune HospitalShanxi Academy of Medical SciencesTaiyuan 030032Shanxi ProvinceChina Department of Psychiatry and Behavioral Neurosciences McMaster UniversityHamilton L8S 4L8ONCanada Department of Psychopharmacology Peking University Sixth HospitalBeijing 100191China Department of Psychiatry Hartford HealthCareSt Vincent’s Medical CenterWestportCT 06088United States

BACKGROUND Musical hallucinations(MH)involve the false perception of music in the absence of external stimuli which links with different *** pathomechanisms of MH encompass various *** etiological classification of MH is of particular importance and offers valuable insights to understand MH,and further to develop the effective treatment of *** the recent decades,more MH cases have been reported,revealing newly identified medical and psychiatric causes of *** imaging studies reveal that MH activates a wide array of brain *** up-to-date analysis on MH,especially on MH comorbid psychiatric conditions is *** To propose a new classification of MH;to study the age and gender differences of MH in mental disorders;and neuropathology of *** Literatures searches were conducted using keywords such as“music hallucination,”“music hallucination and mental illness,”“music hallucination and gender difference,”and“music hallucination and psychiatric disease”in the databases of PubMed,Google Scholar,and Web of *** cases were collected and categorized based on their *** t-test and ANOVA were employed(P<0.05)to compare the age differences of MH different etiological *** neuroimaging studies of neural networks regulating MH and their possible molecular mechanisms were *** Among the 357 yielded publications,294 MH cases were *** average age of MH cases was 67.9 years,with a predominance of females(66.8%females vs 33.2%males).MH was classified into eight groups based on their etiological *** analysis of MH cases indicates varying associations with psychiatric *** We carried out a more comprehensive review of MH *** the first time according to our knowledge,we demonstrated the psychiatric conditions linked and/or associated with MH from statistical,biological and molecular point of view.

关键词： Pathomechanism Etiological factors Classification Gender difference Neuropathway Psychotic musical hallucination and non-psychotic musical hallucination Neuropathway Biological and molecular mechanism

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马克思主义方法论在思政教育研究中的创新

山西财经大学学报 2023年第S2期45卷 133-135页

作者：金正连重庆工业职业技术学院重庆401120

学校在开展思想政治教育活动时,需要转变学生的一些思想政治观念,同时也要对学生的思想观和价值观进行积极的培养。教师要充分借助马克思主义方法论进行教学,提高自己的专业性以及对马克思主义方法论的理解,从而促进思想教育的发展与创新。

关键词：马克思主义方法论思想政治教育创新精神

A self-centering and stiffness-controlled MEMS accelerometer

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Microsystems & Nanoengineering 2024年第1期10卷 27-40页

作者： Yiming jin Zhipeng Ma Ziyi Ye Mingkang Li Xudong zheng Zhonghe jin School of Aeronautics and Astronautics Zhejiang UniversityHangzhou 310013China Key Laboratory of Micro/Nano-Satellite Research of Zhejiang Province Hangzhou 310007PR China

This paper presents a high-performance MEMS accelerometer with a DC/AC electrostatic stiffness tuning capability based on double-sided parallel plates(DSPPs).DC and AC electrostatic tuning enable the adjustment of the effective stiffness and the calibration of the geometric offset of the proof mass,respectively.A dynamical model of the proposed accelerometer was developed considering both DC/AC electrostatic tuning and the temperature *** on the dynamical model,a self-centering closed loop is proposed for pulling the reference position of the forceto-rebalance(FTR)to the geometric center of *** self-centering accelerometer operates at the optimal reference position by eliminating the temperature drift of the readout circuit and nulling the net electrostatic tuning *** stiffness closed-loop is also incorporated to prevent the pull-in instability of the tuned low-stiffness accelerometer under a dramatic temperature ***-time adjustments of the reference position and the DC tuning voltage are utilized to compensate for the residue temperature drift of the proposed *** a result,a novel controlling approach composed of a self-centering closed loop,stiffness-closed loop,and temperature drift compensation is achieved for the accelerometer,realizing a temperature drift coefficient(TDC)of approximately 7μg/℃ and an Allan bias instability of less than 1μg.

关键词： stiffness electrostatic centering

Software Defect Prediction Method Based on Stable Learning

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Computers, Materials & Continua 2024年第1期78卷 65-84页

作者： Xin Fan jingen Mao liangjue lian Li Yu Wei zheng Yun Ge College of Aerospace Engineering Nanjing University of Aeronautics and AstronauticsNanjing210012China School of Software Nanchang Hangkong UniversityNanchang330029China Software Testing and Evaluation Center Nanchang Hangkong UniversityNanchang330029China

The purpose of software defect prediction is to identify defect-prone code modules to assist software quality assurance teams with the appropriate allocation of resources and *** previous software defect prediction studies,transfer learning was effective in solving the problem of inconsistent project data ***,target projects often lack sufficient data,which affects the performance of the transfer learning *** addition,the presence of uncorrelated features between projects can decrease the prediction accuracy of the transfer learning *** address these problems,this article propose a software defect prediction method based on stable learning(SDP-SL)that combines code visualization techniques and residual *** method first transforms code files into code images using code visualization techniques and then constructs a defect prediction model based on these code *** the model training process,target project data are not required as prior *** the principles of stable learning,this paper dynamically adjusted the weights of source project samples to eliminate dependencies between features,thereby capturing the“invariance mechanism”within the *** approach explores the genuine relationship between code defect features and labels,thereby enhancing defect prediction *** evaluate the performance of SDP-SL,this article conducted comparative experiments on 10 open-source projects in the PROMISE *** experimental results demonstrated that in terms of the F-measure,the proposed SDP-SL method outperformed other within-project defect prediction methods by 2.11%-44.03%.In cross-project defect prediction,the SDP-SL method provided an improvement of 5.89%-25.46% in prediction performance compared to other cross-project defect prediction ***,SDP-SL can effectively enhance within-and cross-project defect predictions.

关键词： Software defect prediction code visualization stable learning sample reweight residual network

Clinical observation on 90 cases of primary dysmenorrhea treated by buccal acupuncture therapy:a randomized controlled study

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Journal of Traditional Chinese Medicine 2024年第1期44卷 172-181页

作者： YANG Yi YE Huijun zheng Huiling jin Lihua Department of Obstetrics and Gynecology the Second Affiliated Hospital of Zhejiang Chinese Medical UniversityXinhua Hospital of Zhejiang ProvinceHangzhou 310005China

OBJECTIVE:To explore the short-term and long-term efficacy of buccal acupuncture therapy in the treatment of patients with primary dysmenorrhea(PD).METHODS:A total of 90 patients with PD who were admitted to the Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine from January 2020 to December 2021 were included in this study and randomly divided into three groups,including the observation group,the control group,and the placebo group,with 30 patients in each *** in the observation group were treated according to the syndrome types of Traditional Chinese Medicine,those in the control group were treated with Saridon,and those in the placebo group received a *** visual analog scale(VAS)scores of these patients were evaluated at different time points(before treatment,5 min after needle retention,30 min after needle retention,1 h after treatment,2 h after treatment,1 d after treatment,and 2 d after treatment).The Cox Menstrual Symptom Scale(CMSS)scores were assessed before the treatment and after three courses of ***,these two scales were also evaluated during ***,the pain score and adverse reactions of these patients were assessed during ***:After treatment,the VAS and CMSS scores in the observation group decreased significantly(P<0.05)5 and 30 min after needle retention and after ***:Buccal acupuncture therapy is verified to have short-term efficacy in the treatment of *** addition,this therapy has an immediate analgesic effect and long-term efficacy in reducing the severity and frequency of PD.

关键词： buccal acupuncture therapy primary dysmenorrhea pain score randomized controlled trial

Coupling analysis method of grouting construction with deformation response of adjacent existing tunnel

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Underground Space 2024年第2期15卷 312-330页

作者： Ping-wei Jiang Zhi-hong Zhang Hong zheng jin-kun Huang Key Laboratory of Urban Security and Disaster Engineering of China Ministry of Education Beijing University of TechnologyBeijing 100124China China MCC17 Group Co.LTD MaanshanAnhui 243000China School of Naval Architecture Ocean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

In the new tunnel under close distance through the existing tunnel risk source,the grouting scheme developed to compensate for stratum losses is still based on empirical methods,relying on the overburden thickness of existing *** can potentially lead to an excessively high or low probability of uplift of existing *** a coupled deformation analysis method between the grouting construction and adjacent existing tunnels is of great theoretical significance for developing grouting *** order to reasonably limit the design parameters of grouting construction,based on the theory of fluid–solid coupling elastic pore-column expansion and the theory of random media,the calculation method of stratum displacement which simultaneously considers the coexistence of grout compaction expansion and permeability diffusion mode is derived,and the accuracy of the calculation method is verified by engineering *** accuracy of this calculation method was verified through engineering *** with the deformation coordination condition,the existing tunnel is regarded as an elastic Euler-Bernoulli continuous beam,and the finite element coupling balance equation of the interaction between the existing tunnel and the surrounding soil is *** on this,a coupling calculation model of the grouting construction and the deformation response of the adjacent existing tunnel is *** with three times of grouting construction examples in the shield tunneling project of Beijing Metro Line 12 under the existing airport line,the reliability of the coupling calculation model to determine the grouting construction parameters is *** calculation parameters in the coupling calculation model have clear physical meanings,which can provide a theoretical basis for the grouting design of similar risk source projects.

关键词： Grouting Close-range underpass Tunnel Random medium theory Euler-Bernoulli continuous beam