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Optimization of Cottonseed Meal De-gossypol Process by Extrusion via Genetic Algorithm Based on Artificial Neural Network

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Animal Husbandry and Feed Science 2016年第1期8卷 51-53,57页

作者： Zhang Qiang Wu Hongbin jin xinwen Institute of Agro-Products Processing Science and Technology Xinjiang Academy of Agricultural and Reclamation Science Key Laboratory of Agro-products Processing Science and Technology Xinjiang Academy of Agricultural and Reclamation Science

[ Objective] The paper aimed to optimize cottonseed meal de-gossypol process by extrusion. [ Method ] The artificial neural network （ANN） was used to stimulate the degradation of free gossypol in cottonseed meal by extrusion process, and a three-layer back propagation neural network was established to predict the degradation of free gossypol. [ Result] The result of 10-fold cross validation showed that the ANN with the training function as traingdx at hidden layer with eight neurons gave the smallest mean square error （MSE）. ANN predicted results were very close to the experimental results with correlation coefficient （R2 ） of 0.994 1 and RMSE of 0.497 1. A genetic algorithm （GA） based on the established neural network model was also used for optimizing de-gossypol process. The re- sults of GA showed that the optimal conditions of de-gossypol were puffing temperature 131℃, water ratio 51% , rotational speed 158 r/rain, and feeding speed 136 kg/h, while under this condition the degradation rate of free gossypol was 90.50%, which was close to the predicted result of CA with the small average relative er- ror of 1.38%. [ Conclusion] These results suggested that the GA based on a neural network model might be an excellent tool for optimizing cottonseed meal de-gos- sypol process.

关键词： Cottonseed meal Extrusion De-gossypol Neural network Genetic algorithm

Preliminary Localization of Resistance Gene against Tomato Gray Mold in Xinjiang

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Plant Diseases and Pests 2017年第6期8卷 31-34页

作者： Zhang Qiang Dai Wenting jin xinwen Xinjiang Academy of Agricultural and Reclamation Science

The genetic linkage map of F1 progeny of tomato variety " TS-107" and susceptible cultivar " TS-100" in Xinjiang was constructed. The inheritance of resistance genes against gray mold in disease-resistant cultivar " TS-107" was studied. Genetic mapping of resistance genes through Bulked Segregation Analysis( SSA) and Simple Sequence Recognition( SSR) markers provided germplasm resources for resistance breeding against gray mold. The results showed that the resistance of tomato " TS-107" to gray mold was controlled by a dominant gene. Eleven SSR polymorphic markers were screened and the gene was located between LGVII markers SSR12510 and ECM123 by linkage analysis. There was a partial segregation phenomenon in the No. 327 and No. 328 markers with significant difference,and the two markers had partial segregation sites.

关键词： Tomato Gray mold resistance gene SSR marker Genetic mapping

Effect of Extrusion Temperature on Free Gossypol and Nutritional Components of Cottonseed Meal

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Animal Husbandry and Feed Science 2016年第2期8卷 94-97页

作者： Zhang Qiang Wu Hongbin jin xinwen Institute of Ago-Products Processing Science and Technology Xinjiang Academy of Agricultural and Reclamation Science Shihezi 832000 China Key Laboratory of Agro-products Processing Science and Technology Xinjiang Academy of Agrlcultural and Reclamation Science Shihezi 832000 China

[ Objective] The paper aimed to study effects of extrusion temperature on free gossypol and nutritional components of cottonseed meal. [ Method ] Dif- ferent extrusion temperatures （90, 100, 110, 120 and 130℃ ） were designed, and contents of free gossypol and nutritional components in cottonseed meal were de- termined. [ Result] The optimal temperature for extrusion of cottonseed meal was 120℃, the degradation rate of free gossypol no longer increased with the increas- ing temperature when the temperature exceeded 120℃. The nitrogen solvable index （NSI） in cottonseed meal reduced quickly with the increase of extrusion temper- ature, and the contents of crude fiber, available lysine and total lysine in cottonseed meal decreased significantly, but the contents of total amino acids and essential amino acid reached maximums at the extrusion temperature of 120℃. The content of branched-chain amino acids also reached a maximum at 120℃. [ Conclusion] The extrusion temperature of 120℃ was preferred for lowering free gossypol significantly when maintaining higher nutritional value of cottonseed meal. The paper provided a certain technical basis for extrusion processing of cottonseed meal.

关键词： Cottonseed meal Extrusion temperature Free gossypol Nutritional components

Bioinformatics Analysis on Pathogenic Genes of Botrytis cinerea Pers.

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Agricultural Biotechnology 2018年第1期7卷 57-61,86页

作者： Qiang ZHANG Wenting DAI xinwen jin Xuhui WANG Xinjiang Agricultural Reclamation Academy of Science Shihezi 832000 China Organisms Energy Research Institute in XinJiang Academy of Agriculture Science Urumqi 830091 China

With reported pathogenic genes of Botrytis cirterea Pers. and unknown proteins related to pathogencity as experimental materials, the pathogenic genes were studied by bioinformatics methods, and these unknown proteins were analyzed with bioinforrnaties tools and softwares, so as to lay a theoretical basis for the control of tomato grey mould at molecular level in future. The results showed that through the alignment analysis by DNAMAN software, three pairs of unknown pro- teins with higher comprehensive matching degrees were obtained, and these proteins were predicted to be coded by hrp gene cluster of type Ili secretion system; through Bioedit and ProtParam, the amino acid composition, size, molecular weight, isoelectrie point and hydrophobicity of the each of the six unknown proteins were obtained; and corresponding secondary structures were obtained using DNAMAN. The results of this study could guide deep research of such genes.

关键词： Tomato Grey mould Pathogenic gene Bioinformatics

SSR Molecular Marker for Late Blight Resistance Gene in Tomato CLN2037

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Agricultural Biotechnology 2018年第2期7卷 1-4,14页

作者： Qiang ZHANG Wenting DAI xinwen jin Xuhui WANG Xinjiang Academy of Agricultural and Reclamation Science Organisms Energy Research Institute Xinjiang Academy of Agriculture ScienceUrumqi 830091China

[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] BCr,BCs and F2 were constructed from tomato CLN2037 and susceptible cultivar T2-03,and their resistance to late blight was identified and analyzed by inoculating the disease in the seedling stage of tomatoes. The genetic linkage analysis was made based on the genetic linkage map constructed by ICu GI using group segregation method and 90 pairs of tomato SSR primers. [Results]The resistance of CLN2037 to late blight was controlled by a single recessive gene. The polymorphic bands amplified by primer DM0231 had a linkage relationship with resistance genes. The size of the polymorphism fragment was 226 bp with the genetic linkage distance of 2. 67 c M,and the disease resistance gene was localized on CCPB272-03741.[Conclusions]DM0231 could be used as a molecular marker for molecular breeding of tomato against late blight,which laid the foundation for further fine localization of late blight resistance genes.

关键词： Tomato Late blight Disease-resistant gene SSR molecular markerHome

Isolation and Identification of Botrytis cinerea in Processing Tomato(Solanum lycopersicum)

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Plant Diseases and Pests 2021年第1期12卷 23-26,30页

作者： Li Ziqin Yang Hui Zhang Qiang jin xinwen Xinjiang Academy of Agricultural Reclamation Sciences Xinjiang 832000China

[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey *** identification,it was determined as *** 18S rDNA and internal transcriptional spacer(ITS)sequences of strain nky007a were amplified for molecular identification and classification.[Result]The 18S rDNA sequence of the strain shared the homology of 99.95%with Botryotinia fuckeliana(EF 110887.1);the ITS rDNA sequence had the homology of 100%with Botryotinia fuckeliana(AB444949.1),and there was no difference in *** tree analysis of strain nky007a indicated that nky007a belonged to *** healthy tomatoes were infected by the strain,the incidence rate of tomato grey mold was up to 95%,indicating the pathogenicity of the strain.[Conclusion]Morphological identification,molecular identification and pathogenicity analysis results show that the strain nky007a is a highly pathogenetic strain ***.

关键词： Tomato grey mold Botrytis cinerea Strain isolation Identification

Effect of Different Pretreatments on Explosion Puffing Drying of Hami Melon at Modified Temperature and Pressure in Xinjiang

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Asian Agricultural Research 2016年第9期8卷 64-66,70页

作者： Wenting JIA Hui YANG Hongbin WU Hong WU xinwen jin Institute of Agro-product Processing/Key Laboratory of Agro-product Processing Xinjiang Academy of Agricultural and Reclamation Science

This paper studies the influence of three different pretreatments( blanching,citric acid soaking,and Na Cl soaking) on the moisture content,degree of puffing,color,hardness and crispness of Hami melon under explosion puffing drying at modified temperature and pressure in Xinjiang. The results show that using 60 s blanching pretreatment can help to reduce the moisture content of puffed product while maintaining product color; 2. 5% citric acid pretreatment can make products maintain high degree of puffing and crispness,but reduce product color; 2%Na Cl penetrating fluid plays a significant role in maintaining product color,while maintaining crispness.

关键词： Pretreatment Xinjiang Hami melon Modified temperature and pressure

Screening of Tomato Resources Resistant to Tomato Gray Mold

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Agricultural Biotechnology 2018年第6期7卷 29-31页

作者： Qiang ZHANG Wenting DAI Hongbin WU Hui YANG xinwen jin Institute of Agro-products Processing Science and Technology Xinjiang Academy of Agricultural Reclamation Science

This study was conducted to screen tomato resources resistant to gray mold for the first time by in-vitro stem inoculation *** results showed that Solanum habrochaites T2-07-316 was highly resistant to gray mold,and had the relative stem infection rate and relative stem rot expansion rate of 0 on the 6^(th) day after *** were also other seven transgenic common tomato materials which showed higher resistance to gray mold,with a relative stem infection rate in the range of 15.00%-38.33% and a relative stem rot expansion rate in the range of 10.22%-23.57%.Among them,T2-07-337 had the best resistance.

关键词： Tomato Gray mold Screening of resistant resources In-vitro stem inoculation

Genetic Analysis of Resistance to Tomato Gray Mold( Botrytis cinerea)

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Agricultural Biotechnology 2019年第1期8卷 40-42页

作者： Qiang ZHANG Wenting DAI Hongbin WU Hui YANG xinwen jin Institute of Agro-products Processing Science and Technology Xinjiang Academy of Agricultural Reclamation Science

The study used four tomato varieties,03786( susceptible),05237( susceptible),C2985( resistant) and Wva536( resistant) with different resistance,to formulate combinations according to Griffing( I) complete diallel cross method. And Botrytis cinerea was inoculated to investigate the incidence of gray mold. The results showed that:(1) there were significant differences in disease resistance between different varieties and different combinations.(2) There were significant differences in general combining ability and special combining ability between the tested varieties,and Wva536 and C2985 had a higher general combining ability effect,and were both relatively good parents when formulating resistant combinations. Combinations Wva536 × 03786 and Wva536 × 05237 had the highest special combining ability,and thus have the value of further research and utilization.(3) The additive effect in resistance inheritance was located at the primary position,and partial dominance also existed as well as cytoplasmic effect.(4) The broad-sense heritability and narrow-sense heritability were,respectively,89. 73% and82. 15%,which were higher,indicating that the parents had a greater effect on offsprings. The resistance genes can be expressed in offsprings through gene accumulation,and should be selected in the early generation.

关键词： Tomato Diallel cross Tomato gray mold Combining ability Genetical analysis of resistance