Isolation and Identification of Botrytis cinerea in Processing Tomato(Solanum lycopersicum)

作     者：Li Ziqin Yang Hui Zhang Qiang Jin Xinwen 

作者机构：Xinjiang Academy of Agricultural Reclamation SciencesXinjiang 832000China 

出 版 物：《Plant Diseases and Pests》 (植物病虫害研究（英文版）)

年 卷 期：2021年第12卷第1期

页      面：23-26,30页

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：Science and Technology Guidance Program of Xinjiang Academy of Agricultural Reclamation Sciences(2020YJ019) 

主　　题：Tomato grey mold Botrytis cinerea Strain isolation Identification 

摘      要：[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey *** identification,it was determined as *** 18S rDNA and internal transcriptional spacer(ITS)sequences of strain nky007a were amplified for molecular identification and classification.[Result]The 18S rDNA sequence of the strain shared the homology of 99.95%with Botryotinia fuckeliana(EF 110887.1);the ITS rDNA sequence had the homology of 100%with Botryotinia fuckeliana(AB444949.1),and there was no difference in *** tree analysis of strain nky007a indicated that nky007a belonged to *** healthy tomatoes were infected by the strain,the incidence rate of tomato grey mold was up to 95%,indicating the pathogenicity of the strain.[Conclusion]Morphological identification,molecular identification and pathogenicity analysis results show that the strain nky007a is a highly pathogenetic strain ***.