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A Method for Truck Frame Strength Analysis with Simplified Suspension Model

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A Method for Truck Frame Strength Analysis with Simplified S...

IFN-α可以通过抑制miR-181 a水平间接调控SAMHD1的表达

2015中国汽车工程学会年会

作者： changzhong jin Pengbo Wang Beijing Chang’an Auto R＆D Center Chang’an Automobile Co.Ltd.

The truck frame is an important part bearing loads from engine,chassis,body and other *** strength analysis method considers the frame alone,so the stress result cannot reflect the effect of leaf spring deformation under *** paper studies a light truck *** equivalent beam method,frame and simplified suspension model were built to execute strength analysis for bending,torsion,braking and steering cases.A structure optimization scheme is applied for the high-stress regions,which provides a reference for truck frame design.

关键词： frame equivalent beam method strength analysis structure optimization

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中华微生物学和免疫学杂志 2016年第4期36卷 277-280页

作者：饶和平王炜金祥宁靳昌忠衢州职业技术学院医学院 324000 衢州市疾病预防控制中心艾滋病实验室 324000 金华职业技术学院医学院 321000 浙江大学医学院附属第一医院传染病诊治国家重点实验室 310003

目的：研究IFN-α对不育-α-基序结构域和组氨酸/天冬氨酸残基双联体结构域包涵蛋白1（sterile alpha motif and histidine/aspartic acid domain containing protein 1, SAMHD1）表达的诱导,以及miR-181a在此过程中的作用。方法以THP-1和Jurkat细胞为细胞模型,用不同剂量 IFN-α（200 IU/ml和1000 IU/ml）处理后定量 PCR检测 SAMHD1 mRNA 和 miR-181a 的表达水平,并用Western blot检测SAMHD1蛋白的表达水平。转染miR-181a过表达质粒p-181a后再用IFN-α（200 IU/ml）处理细胞,检测SAMHD1表达量的变化。结果 IFN-α可以显著增加SAMHD1 mRNA和蛋白水平的表达,同时抑制miR-181a的表达,尤其以Jurkat细胞结果更为显著。过表达miR-181a能够显著降低IFN-α诱导的SAMHD1表达。结论 IFN-α可以通过抑制miR-181a水平间接调控SAMHD1的表达。

关键词： SAMHD1 IFN-α miR-181 a HIV感染炎症反应

Human cellular miR-1224-3p and miR-1913 seed family microRNAs target on HIV-1 and inhibit virus production

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Detection of the long non-coding RNAs NEAT1 and MALAT1 in the peripheral blood of HIV-1 infected patients

中华医学会第七次全国艾滋病、丙型肝炎学术会议

作者： Lu Xiangyun Yang jin jin changzhong Cheng Linfang Wu Haibo Wang Juan Yang Zongxing Liu Fumin Peng Xiaorong Xie Tiansheng Yao Hangping Wu Nanping State Key Laboratory for Diagnosis and Treatment of Infectious Disease Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseThe First Affiliated HospitalCollege of MedicineZhejiang University

Objective:Increasing evidences indicate that both cellular and viral derived miRNAs are important players of host-virus *** decide to search for novel cellular miRNAs that can directly target on the HIV-1 transcripts and function to repress HIV-1 ***:The terminal 1.9kb of the 3-UTR of HIV-1 YU2 strain and subfragments were cloned to luciferase and GFP-based miRNA re-

关键词： HIV miRNAs miR microRNAs Human cellular miR-1224-3p and miR-1913 seed family microRNAs target on HIV-1 and inhibit virus production

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Detection of the long non-coding RNAs NEAT1 and MALAT1 in th...

HIV-1感染者免疫活化对宿主限制因子SAMHD1表达和HIV-1 DNA水平的影响

中华医学会第七次全国艾滋病、丙型肝炎学术会议

作者： jin changzhong Peng Xiaorong Xie Tiansheng Lu Xiangyun Liu Fumin Wu Haibo Yang Zongxing Wang Juan Cheng Linfang Wu Nanping State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalSchool of MedicineZhejiang University Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

Objectives:The Long non-coding RNAs(lncRNAs)in HIV-1 infection are not well *** we detected two lncRNAs,NEAT1 and MALAT1,in peripheral blood mononuclear cells(PBMC)and plasma of HIV-1 infected ***:Fifty nine HIV-1 infected patients and 21 healthy controls were recruited,of whom 31 patients were HAART-na?ve and 28 patients received HAART for more

关键词： HIV HAART Detection of the long non-coding RNAs NEAT1 and MALAT1 in the peripheral blood of HIV-1 infected patients

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实用医学杂志 2021年第22期37卷 2862-2865页

作者：饶和平姚水洪王炜靳昌忠衢州职业技术学院医学院“衢州市病毒致瘤研究科技创新团队” 浙江衢州324000 衢州市疾病预防控制中心艾滋病实验室浙江衢州324000 浙江大学医学院附属第一医院传染病诊治国家重点实验室杭州310003

目的研究HIV-1感染者CD4;T细胞免疫活化与外周血宿主限制因子SAMHD1表达水平和HIV-1 DNA的关系。方法选取未接受抗病毒治疗的HIV-1感染者57例,接受抗病毒治疗且病毒载量低于检测限的HIV-1感染者62例,健康对照48例。分离外周血单个核细胞... 详细信息

目的研究HIV-1感染者CD4;T细胞免疫活化与外周血宿主限制因子SAMHD1表达水平和HIV-1 DNA的关系。方法选取未接受抗病毒治疗的HIV-1感染者57例,接受抗病毒治疗且病毒载量低于检测限的HIV-1感染者62例,健康对照48例。分离外周血单个核细胞(PBMC)和CD4;T细胞,提取总RNA,RT-PCR法检测SAMHD1 mRNA表达水平及血浆HIV-1 RNA水平,流式细胞术检测CD4;T和CD8;T细胞数量及CD4;T细胞免疫活化水平,ELISA检测血浆干扰素α水平,PCR法检测外周血HIV-1 DNA水平。结果未接受抗病毒治疗的HIV-1感染者CD4;T细胞免疫活化水平最高,抗病毒治疗组显著降低,但仍高于健康对照组(P <0.01)。未接受抗病毒治疗组外周血HIV-1 DNA水平显著高于抗病毒治疗组(P <0.01)。HIV-1感染者PBMCs中SAMHD1 mRNA水平显著高于健康对照组(P <0.01),而CD4;T细胞中SAMHD1 mRNA表达水平显著低于健康对照组(P <0.01)。CD4;T细胞SAMHD1 mRNA表达水平与免疫活化呈负相关,但与HIV-1 DNA水平无相关性。结论 HIV-1感染者CD4;T细胞SAMHD1表达水平降低,与免疫活化水平负相关,但与HIV-1 DNA水平无直接相关。

关键词： SAMHD1 HIV-1 HIV-1 DNA 免疫活化抗逆转录病毒治疗

Shor's algorithm does not factor large integers in the presence of noise

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Science China(Information Sciences) 2024年第7期67卷 305-320页

作者： jin-Yi CAI College of Letters and Science University of Wisconsin

We consider Shor's quantum factoring algorithm in the setting of noisy quantum gates. Under a generic model of random noise for(controlled) rotation gates, we prove that the algorithm does not factor integers of the form pq when the noise exceeds a vanishingly small level in terms of n—the number of bits of the integer to be factored, where p and q are from a well-defined set of primes of positive density. We further prove that with probability 1-o(1) over random prime pairs(p, q), Shor's factoring algorithm does not factor numbers of the form pq, with the same level of random noise present.

关键词： random noise Shor's algorithm rotation gates quantum computing prime factorization

Semi-analytical Solutions for Wellbores with Graded Skin Zones in Poroelastic Media

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Acta Mechanica Solida Sinica 2024年第3期37卷 385-395页

作者： Zhiqiang Fan Zhihe jin School of Mechanics Civil Engineering and ArchitectureNorthwestern Polytechnical UniversityXi’an710072China Department of Mechanical Engineering University of MaineOronoME04469USA

In analyzing the complex interaction between the wellbore and the reservoir formation,the hydromechanical properties of the region proximal to the wellbore,referred to as the“wellbore skin zone”,play a pivotal role in determining flow dynamics and the resulting formation *** models of the wellbore skin zone generally assume a constant permeability throughout,resulting in a sharp permeability discontinuity at the skin-reservoir *** paper introduces a model for a wellbore with a continuously graded skin zone of finite thickness within a poroelastic *** solutions are derived using the Laplace transform method,addressing both positive and negative skin *** results are presented to illustrate the effects of graded permeability/skin zone thickness on pore pressures and stresses around a *** results highlight a distinct divergence in stress and pore pressure fields when comparing wellbores with negative skin zones to those with positive skin zones or no skin at all.

关键词： Poroelastic Graded permeability Analytical solution Wellbore Skin zone

Advanced Algorithm for Parameters Estimation of Negative Binomial Distribution with High Dimensional Sparse Group Structure

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Journal of Systems Science & Complexity 2024年第5期37卷 2173-2195页

作者： LI Meiqi jin Baisuo School of Management University of Science and Technology of China

Negative binomial regression is a powerful technique for modeling count data, particularly when dealing with overdispersion. However, estimating the parameters for large-dimensional sparse models is challenging due to the complexity of optimizing the mean and dispersion parameter of the negative binomial distribution. To address this issue, the authors propose a novel approach that employs two iterations of the majorize-minimize(MM) algorithm, one for estimating the dispersion parameter and the other for estimating the mean parameters. These approaches improve the convergence speed and stability of the algorithm. The authors also use group penalty for variable selection, which enhances the accuracy and efficiency of the algorithm. The proposed method provides an explicit solution,simplifies the iteration process, and maintains good stability while ensuring algorithm ***, the authors apply the proposed algorithm to the zero-inflated model and demonstrate its promising predictive performance on specific data sets. The research has important implications for count data modeling and analysis in various fields, such as data mining, machine learning, and bioinformatics.

关键词： Group structure majorize-minimize (MM) negative binomial

Combined Treatment with Bojungikgi-tang(Buzhong Yiqi Decoction)and Riluzole Attenuates Cell Death in TDP-43-Expressing Cells

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Chinese Journal of Integrative Medicine 2024年第7期30卷 616-622页

作者： Eun jin Yang Department of KM Science Research Korea Institute of Oriental MedicineDaejeonYuseong-gu34054Republic of Korea

Objective To examine the effect of combined treatment with Bojungikgi-tang(BJIGT,Buzhong Yiqi Decoction)and riluzole(RZ)in transactive response DNA-binding protein 43(TDP-43)stress granule(SG)cells,a amyotrophic lateral sclerosis(ALS)cell line using transcriptomic and molecular *** TDP-43 SG cells were pretreated with BJIGT(100µg/mL),RZ(50µmol/L),and combined BJIGT(100µg/mL)/RZ(50µmol/L)for 6 h before treatment with lipopolysaccharide(LPS,200µmol/L).Cell viability assay was performed to elucidate cell toxicity in TDP-43 SC cells using a cell-counting kit-8(CCK8)assay *** expression levels of cell death-related proteins,including Bax,caspase 1,cleaved caspase 3 and DJ1 in TDP-43 SG cells were examined by Western blot *** autophagy-related proteins,including pmTOR/mTOR,LC3b,P62,ATG7 and Bcl-2-associated athanogene 3(Bag3)were investigated using immunofluorescence and immunoblotting *** Cell viability assay and Western blot analysis showed that combined treatment with BJIGT and RZ suppressed LPS-induced cell death and expression of cell death-related proteins,including Bax,caspase 1,and DJ1(P<0.05 or P<0.01).Immunofluorescence and immunoblotting assays showed that combined treatment with BJIGT and RZ reduced LPS-induced formation of TDP-43 aggregates and regulated autophagy-related protein levels,including p62,light chain 3b,Bag3,and ATG7,in TDP-43-expressing cells(P<0.05 or P<0.01).Conclusion The combined treatment of BJIGT and RZ might reduce inflammation and regulate autophagy dysfunction in TDP-43-induced ALS.

关键词： Bojungikgi-tang Buzhong Yiqi Decoction transactive response DNA-binding protein 43 autophagy oxidative stress amyotrophic lateral sclerosis