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Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of <i>Leishmania</i>Genus Parasites

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American Journal of Analytical Chemistry 2013年第7期4卷 100-108页

作者： Pedro Rodríguez Humberto Rojas Miguel Medina Jazzmin Arrivillaga Yosue Francisco Fracehuli Dager Vincent Piscitelli Manuel Caetano Alberto Fernández jimmy castillo Centro de Microscopia Electrónica Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela Departamento de Biología Universidad Simón Bolívar Caracas Venezuela Escuela de Biología Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela Laboratorio de Espectroscopia Laser Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela

In this work we present the preparation and functionalization of gold nanoparticles (AuNPs) for the detection of presence of gp63 glycoprotein in the surface of Leishmania genus parasites. AuNPs were prepared by induced laser ablation in a clean and biologically suitable media. The nanoparticles were functionalized with anti-gp63 lgG antibody in order to study the interaction with the glycoprotein component gp63 (63 kDa) present on the membrane surface of Leishmania genus parasites. The functionalized AuNPs showed potential as a spectrometric indicator of the parasite existence, both by the detection of the presence of the gp63 in solution and through the specific interaction with the parasites in vitro. The specificity of the study opens a new line of research on the use of modified nanoparticles in the development of a fast and easy assay for Leishmaniosis diagnostics.

关键词： Leishmania Nanoparticles Surface Plasmon Resonance gp63 Protein

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A Prototype of a Semantic Platform with a Speech Recognition System for Visual Impaired People

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Journal of Intelligent Learning Systems and Applications 2015年第4期7卷 87-92页

作者： jimmy Rosales-Huamaní José castillo-Sequera Fabricio Puente-Mansilla Gustavo Boza-Quispe National University of Engineering Lima Peru Alcala University Madrid Spain

In the world, 10% of the world population suffer with some type of disability, however the fast technological development can originate some barriers that these people have to face if they want to access to technology. This is particularly true in the case of visually impaired users, as they require special assistance when they use any computer system and also depend on the audio for navigation tasks. Therefore, this paper is focused on making a prototype of a semantic platform with web accessibility for blind people. We propose a method to interaction with user through voice commands, allowing the direct communication with the platform. The proposed platform will be implemented using Semantic Web tools, because we intend to facilitate the search and retrieval of information in a more efficient way and offer a personalized learning. Also, Google APIs (STT (Speech to Text) and TTS (Text to Speech)) and Raspberry Pi board will be integrated in a speech recognition module.

关键词： Semantic Web Ontology ASR Raspberry Pi

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