Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of <i>Leishmania</i>Genus Parasites

作     者：Pedro Rodríguez Humberto Rojas Miguel Medina Jazzmin Arrivillaga Yosue Francisco Fracehuli Dager Vincent Piscitelli Manuel Caetano Alberto Fernández Jimmy Castillo 

作者机构：Centro de Microscopia Electrónica Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela Departamento de Biología Universidad Simón Bolívar Caracas Venezuela Escuela de Biología Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela Laboratorio de Espectroscopia Laser Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela 

出 版 物：《American Journal of Analytical Chemistry》 (美国分析化学（英文）)

年 卷 期：2013年第4卷第7期

页      面：100-108页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Leishmania Nanoparticles Surface Plasmon Resonance gp63 Protein 

摘      要：In this work we present the preparation and functionalization of gold nanoparticles (AuNPs) for the detection of presence of gp63 glycoprotein in the surface of Leishmania genus parasites. AuNPs were prepared by induced laser ablation in a clean and biologically suitable media. The nanoparticles were functionalized with anti-gp63 lgG antibody in order to study the interaction with the glycoprotein component gp63 (63 kDa) present on the membrane surface of Leishmania genus parasites. The functionalized AuNPs showed potential as a spectrometric indicator of the parasite existence, both by the detection of the presence of the gp63 in solution and through the specific interaction with the parasites in vitro. The specificity of the study opens a new line of research on the use of modified nanoparticles in the development of a fast and easy assay for Leishmaniosis diagnostics.