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Plant-derived nanovesicles: Further exploration of biomedical function and application potential

Acta Pharmaceutica Sinica B 2023年第8期13卷 3300-3320页

作者： Aixue Li Dan Li Yongwei Gu Rongmei Liu Xiaomeng Tang Yunan Zhao Fu Qi jifu wei Jiyong Liu College of Pharmacy Shandong University of Traditional Chinese MedicineJinan 250355China Department of Pharmacy Fudan University Shanghai Cancer CenterShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Department of Pharmacy Jiangsu Cancer HospitalNanjing 210009China Jiangsu Institute of Cancer Research Nanjing 210009China The Affiliated Cancer Hospital of Nanjing Medical University Nanjing 210009China Department of Pharmacy Shanghai Proton and Heavy Ion CenterShanghai 201315China

Extracellular vesicles(EVs)are phospholipid bilayer vesicles actively secreted by cells,that contain a variety of functional nucleic acids,proteins,and lipids,and are important mediums of intercellular *** on their natural properties,EVs can not only retain the pharmacological effects of their source cells but also serve as natural delivery *** them,plant-derived nanovesicles(PNVs)are characterized as natural disease therapeutics with many advantages such as simplicity,safety,eco-friendliness,low cost,and low toxicity due to their abundant resources,large yield,and low risk of immunogenicity in *** review systematically introduces the biogenesis,isolation methods,physical characterization,and components of PNVs,and describes their administration and cellular uptake as therapeutic *** highlight the therapeutic potential of PNVs as therapeutic agents and drug delivery carriers,including anti-inflammatory,anticancer,wound healing,regeneration,and antiaging properties as well as their potential use in the treatment of liver disease and ***,the toxicity and immunogenicity,the current clinical application,and the possible challenges in the future development of PNVs were *** expect the functions of PNVs to be further explored to promote clinical translation,thereby facilitating the development of a new framework for the treatment of human diseases.

关键词： Plant-derived nanovesicles Extracellular vesicles Therapeutic agents Drugdelivery systems Clinical application

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Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer

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Chinese Chemical Letters 2022年第4期33卷 1855-1860页

作者： Yongxin Liu Mengda Cao Zhongxi Huang Changmin Yu Naidi Yang Qiong Wu Liang Shi Wenjie Duan Yi Zhu jifu wei Lin Li wei Huang Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816China Research Division of Clinical Pharmacology&Clinical Allergy Center the First Affiliated Hospital of Nanjing Medical UniversityDepartment of PharmacyJiangsu Institute of Cancer Research&the Affiliated Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Department of Pharmacy Zhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210044China State Key Laboratory of Coordination Chemistry Nanjing UniversityNanjing 210023China Shaanxi Institute of Flexible Electronics(SIFE) Northwestern Polytechnical University(NPU)Xi’an 710072China The First Affiliated Hospital of USTC Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China

Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.

关键词： Gold nanoparticles Magnetic nanocapturer IgE detection Ultrasensitive immunosensor Cancer

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Glutathione depletion based Pt(Ⅳ) hybrid mesoporous organosilica delivery system to conquer cisplatin chemoresistance: A “one stone three birds” strategy

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Chinese Chemical Letters 2024年第10期35卷 278-285页

作者： Linjie Ju Zhongxi Huang Qian Shen Chan Fu Shuanghe Li Wenjie Duan Chenfeng Xu weizhen An Zhiqiang Zhai jifu wei Changmin Yu Guoren Zhou Department of Pharmacy Jiangsu Cancer HospitalThe Affiliated Cancer Hospital of Nanjing Medical UniversityJiangsu Institute of Cancer ResearchNanjing210009China Key Laboratory of Flexible Electronics(KLOFE) School of Flexible Electronics(Future Technologies)&Institute of Advanced Materials(IAM)Nanjing Tech University(NanjingTech)Nanjing211816China Department of Oncology Jiangsu Cancer HospitalThe Affiliated Cancer Hospital of Nanjing Medical UniversityJiangsu Institute of Cancer ResearchNanjing 210009China Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization College of Resources and Environmental SciencesNanjing AgriculturalUniversityNanjing210095China

The occurrence of acquired resistance to cisplatin(DDP)that induces the toxic drug effects has always been a huge challenge and urgently needs to be resolved in the cancer *** combination of anticancer drugs with different mechanisms can remarkably improve the chemotherapeutic *** that glutathione(GSH)plays as the driving factors in the resistance of DDP,here we have firstly proposed a“three birds,one stone”based nanoplatform to achieve triple synergetic effects simultaneously addressing DDP resistance in non-small cell lung cancer(NSCLC).Specifically,we initially designed and synthesized a DDP prodrug[Pt(Ⅳ)]bridged silsesquioxane precursor(Pt-Si).Then Pt-Si and bis[3-(triethoxysilyl)propyl]diselenide(BTESe PD)were integrated into the framework of mesoporous organosilica nanoparticles(MONs)to obtain a nanocarrier MONPt/*** loading with norcantharidin(NCTD)and modifying with the aptamer AS1411 based G-quadruplex(Apt),the Apt@NCTD@MONPt/Seexhibit impressive tumor homing *** being endocytosed,(Ⅰ)the diselenide and-O-Pt(Ⅳ)-O-rich scaffold can be reduced by the excessive GSH,followed by(Ⅱ)breaking the redox homeostasis via GSH depletion and precise release of the ***,the encapsulated NCTD is also released along with the degradation of the nanocarriers thereby(Ⅲ)achieving the GSH depletion and synergistic anti-tumor effect of NCTD and *** together,we believe this“one stone,three birds”strategy may be a promising paradigm to conquer drug resistance for clinical care.

关键词： MON_(ptse) NSCLC Glutathione depletion Responsive biodegradation Traditional Chinese medicines Synergistictherapy

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VCAM1基因rs2392221单核苷酸多态性与肾移植术后急性排异发生的相关性

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南京医科大学学报（自然科学版） 2022年第4期42卷 518-521页

作者：刘小华魏继福南京医科大学第一附属医院药学部江苏南京210029 南京医科大学附属肿瘤医院药学部江苏南京210000

目的:阐述血管细胞黏附分子1(vascular cell adhesion molecule 1,VCAM1)基因rs2392221单核苷酸多态性(single nucleotide polymorphism,SNP)与肾移植后急性排异(acute rejection,AR)发生的相关性。方法:回顾性分析南京医科大学第一附属医院200例肾移植患者,根据肾移植术后是否发生急性排异分为急性排异(acute rejection,AR)组69例和非AR组131例。通过二代测序对VCAM1 rs2392221基因型进行检测。采用5个遗传模型分析VCAM1 rs2392221对AR的影响。结合临床和人口统计学数据,评估VCAM1 rs2392221基因多态性与肾移植术后急性排异的关联。结果:5个遗传模型研究显示VCAM1 rs2392221与肾移植术后急性排异显著相关,共显性模型CC ***:OR=3.71,95%CI:1.82~7.58;CC ***:OR=5.41,95%CI:1.14~25.75,P<0.001;显性模型OR=3.90,95%CI:1.97~7.72,P<0.001;隐性模型OR=3.57,95%CI:0.77~16.54,P=0.357;超显性模型OR=3.31,95%CI:1.65~6.65,P=0.002;加性模型OR=3.04,95%CI:1.70~5.43,P=0.034。结论:VCAM1 rs2392221与肾移植术后急性排异的发生显著相关,检测VCAM1 rs2392221可能有助于早期诊断急性排异的发生。

关键词：肾移植单核苷酸多态性急性排异

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A new free-hand pedicle screw placement technique with reference to the supraspinal ligament

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The Journal of Biomedical Research 2014年第1期28卷 64-70页

作者： Juming Li Hong Zhao Hao Xie Lipeng Yu jifu wei Min Zong Feng Chen Ziqiang Zhu Ning Zhang Xiaojian Cao Department of Orthopaedics the First Affiliated Hospital of Nanjing Medical University Department of radiology the First Affiliated Hospital of Nanjing Medical University Department of Statistics Nanjing Medical University

We sought to compare the safety and accuracy of a new free-hand pedicle screw placement technique to that of the conventional technique. One hundred fifty-three consecutive adult patients with simple fracture in the tho- racic or/and lumbar spine were alternately assigned to either the new free-hand or the conventional group. In the new free-hand technique group, preoperative computerized tomography （CT） images were used to calculate the targeted medial-lateral angle of each pedicle trajectory and the pedicle screw was inserted perpendicular to the corresponding supraspinal ligament. In the conventional technique group, the medial-lateral and cranial-caudal angle of each pedicle trajectory was determined by intraoperatively under fluoroscopic guidance. The accuracy rate of pedicle screw placement, the time of intraoperative fluoroscopy, the operating time and the amount of blood loss during operation were respectively compared. All screws were analyzed by using intraoperative ra- diographs, intraoperative triggered electromyography （EMG） monitoring data, postoperative CT data and clinical outcomes. The accuracy rate of pedicle screw placement in the new free-hand technique group and the conven- tional technique group was 96.3% and 94.2% （P 〈 0.05）, respectively. The intraoperative fluoroscopy time of the new technique group was less than that of the conventional technique group （5.37 seconds vs. 8.79 seconds, P 〈 0.05）. However, there was no statistical difference in the operating time and the amount of blood loss during op- eration （P 〉 0.05）. Pedicle screw placement with the free-hand technique which keeps the screw perpendicular to the supraspinal ligament is an accurate, reliable and safe technique to treat simple fracture in the thoracic or lum- bar spine.

关键词： spine fracture pedicle screw placement radiation exposure supraspinal ligament anatomy reference

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Adaptive Evolution of the Spike Gene of SARS Coronavirus: Changes in Positively Selected Sites at Different Epidemic Phases

Adaptive Evolution of the Spike Gene of SARS Coronavirus: Ch...

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2006中国微生物学会第九次全国会员代表大会暨学术年会

作者： Chiyu Zhang, jifu wei, Shaoheng He Department of Biochemistry and Molecular Biology, Jiangsu University School of Medical Technology, Zhenjiang, Jiangsu 212001, China First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China

Background: It was believed that transmission of severe acute respiratory syndrome (SARS) coronavirus (CoV) from animals to humans is the cause of the SARS outbreak worldwide. The spike (S) protein is one of the best characterized proteins of SARS-CoV, and plays a key role in SARS-CoV overcoming the species barrier and accomplishing interspecies transmission from animals to human, suggesting that it

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463例大环内酯类抗生素不良反应分析

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中国药业 2014年第9期23卷 43-45页

作者：姚杰韩莉魏继福南京医科大学第一附属医院药学部江苏南京210029

目的分析大环内酯类抗生素引起的不良反应(ADR)发生的一般规律和特点,促进临床合理用药。方法回顾性分析2003年至2012年收集的463例大环内酯类抗生素ADR报表,按照引起ADR的大环内酯类的品种、患者的性别和年龄、ADR涉及的器官/系统及临... 详细信息

目的分析大环内酯类抗生素引起的不良反应(ADR)发生的一般规律和特点,促进临床合理用药。方法回顾性分析2003年至2012年收集的463例大环内酯类抗生素ADR报表,按照引起ADR的大环内酯类的品种、患者的性别和年龄、ADR涉及的器官/系统及临床表现等进行统计、分析。结果 463例大环内酯类抗生素致ADR所涉及的药物种类有7种;ADR以中老年患者所占比例最大,女性的比例高于男性,主要ADR临床表现有过敏反应、皮疹、消化系统反应等,共引起29例严重ADR,阿奇霉素引起ADR发生例数高于罗红霉素和克拉霉素。结论医师、药师、护士应密切合作,规范诊疗和用药,严格掌握大环内酯类药物的适应证,重视对大环内酯类ADR的用药监护,以减少ADR的发生。

关键词：大环内酯类药品不良反应合理用药

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《国家三级公立医院绩效考核操作手册(2022版)》药事管理指标解读及思考

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中国药房 2022年第13期33卷 1541-1547页

作者：徐思露吴楠蔡宁赵敏潘杰魏继福江苏省肿瘤医院/江苏省肿瘤防治研究所/南京医科大学附属肿瘤医院药学部南京210009 苏州大学附属第二医院药剂科江苏苏州215004

目的解读《国家三级公立医院绩效考核操作手册(2022版)》(以下简称《手册(2022版)》)中关于药事管理的指标修订,为医院推动新版绩效考核方案中药事管理部分的落地与执行提供参考。方法简述《手册(2022版)》药事管理指标内容及修订明细,... 详细信息

目的解读《国家三级公立医院绩效考核操作手册(2022版)》(以下简称《手册(2022版)》)中关于药事管理的指标修订,为医院推动新版绩效考核方案中药事管理部分的落地与执行提供参考。方法简述《手册(2022版)》药事管理指标内容及修订明细,并对修订内容进行解读,提出相关建议。结果与结论《手册(2022版)》延续《手册(2020版)》的绩效考核范围、指标架构和顺序,聚焦合理用药与药品费用控制。《手册(2022版)》更加强调加强基本药物、集中采购中选药品的配备使用,以及进一步减轻患者医药费用负担等内容。建议三级公立医院科学设置临床科室基本药物、集中采购中选药品、辅助用药、抗菌药物使用指标,完善相关激励机制和绩效考核制度;加强基本药物、集中采购政策解读和合理用药宣传培训;优化院内用药目录和药品处方集;通过制定用药规范,加大处方审核、合理用药点评和考核力度;运用信息化手段建立和完善药品使用监测评价和预警制度以规范临床用药行为;在保证合理的基础上加强基本药物、集中采购中选药品的使用;通过合理用药管控以控制药品费用不合理增长,使次均药品费用增幅逐步降低。

关键词：三级公立医院绩效考核药事管理指标基本药物药品集中采购

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生物催化技术在阿托伐他汀手性侧链合成中的应用

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科技通报 2013年第3期29卷 80-87页

作者：魏积福毛芳芳王亚军郑裕国浙江工业大学生物与环境工程学院生物工程研究所杭州310014

他汀类药物是一类20世纪80年代上市的羟甲戊二酰辅酶A还原酶(HMG-CoA)抑制剂类药物,广泛用于临床治疗骨质疏松、高胆固醇血症。近年来,他汀类药物还统治着全球降胆固醇药物市场。自2006年以来,仅阿托伐他汀的销售额就突破百亿美元,是全... 详细信息

他汀类药物是一类20世纪80年代上市的羟甲戊二酰辅酶A还原酶(HMG-CoA)抑制剂类药物,广泛用于临床治疗骨质疏松、高胆固醇血症。近年来,他汀类药物还统治着全球降胆固醇药物市场。自2006年以来,仅阿托伐他汀的销售额就突破百亿美元,是全球销量最高的降血脂药物。随着生物技术、酶工程的持续发展,生物催化已成为手性药物制造的关键技术。本文综述了近10年来酶法不对称合成和化学-酶法组合催化在阿托伐他汀手性药效基团合成中的应用进展,并分析了各工艺的技术优势及存在的问题。

关键词：阿托伐他汀降血脂药物药效基团手性技术生物催化

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基于SERVQUAL模型建立消化道肿瘤围手术期临床药学服务质量评价指标体系的研究

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实用药物与临床 2024年第2期27卷 121-126页

作者：李双鹤魏继福黄凌莉江苏省肿瘤医院江苏省肿瘤防治研究所南京医科大学附属肿瘤医院药学部江苏南京210009 中国药科大学基础医学与临床药学学院江苏南京211198

目的构建适合消化道肿瘤手术患者的临床药学服务质量评价指标体系。方法在SERVQUAL模型的基础上建立适合于消化道肿瘤围手术期临床药学服务质量评价的指标体系,应用德尔菲法(Delphi法)对16名专家进行两轮咨询。计算问卷回收率、各条目... 详细信息

目的构建适合消化道肿瘤手术患者的临床药学服务质量评价指标体系。方法在SERVQUAL模型的基础上建立适合于消化道肿瘤围手术期临床药学服务质量评价的指标体系,应用德尔菲法(Delphi法)对16名专家进行两轮咨询。计算问卷回收率、各条目的均值、标准差、满分比、变异系数,以及专家权威程度和专家协调系数,并对结果进行分析后,形成最终的用于评价消化道肿瘤围手术期临床药学服务质量评价指标体系。结果两轮咨询的专家积极系数均为100%,专家权威系数分别为0.75和0.89。第二轮专家咨询中,一、二、三级指标专家协调程度分别为0.669、0.456和0.505。通过对指标的筛选与修改,最终形成了由5个一级指标、15个二级指标、33个三级指标构成的评价指标体系。结论本研究中,专家的权威程度较高,意见趋于一致,具有较好的专家协调性。评价指标体系的结果较为科学、合理和可靠,能够为评价消化道肿瘤围手术期的临床药学服务质量提供参考依据。

关键词： SERVQUAL模型临床药学消化道肿瘤围手术期服务质量评价体系

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