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QTL and candidate genes associated with common bacterial blight resistance in the common bean cultivar Longyundou 5 from China

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The Crop Journal 2016年第5期4卷 344-352页

作者： jifeng zhu Jing Wu Lanfen Wang Matthew W.Blair Zhendong zhu Shumin Wang Institute of Crop Science Chinese Academy of Agricultural Sciences Department of Agriculture & Environment Tennessee State University

Common bacterial blight(CBB), caused by Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans(Xff), is a worldwide disease of common bean(Phaseolus vulgaris L.).Longyundou 5, a Chinese cultivar in the Mesoamerican gene pool of common bean, displays resistance to the Xff strain XSC3-1. To identify the genetic mechanisms behind this resistance,we crossed Long 5 with a susceptible genotype to develop a mapping population of F2 *** resistance to CBB was identified at 14 and 21 days after inoculation with Xff strain XSC3-1.A major QTL at 14 and 21 days after inoculation was mapped on chromosome Pv10 with LOD scores of 6.41 and 5.35, respectively. This locus was associated with SAP6, a previouslyidentified and much-used dominant marker, but in a 4.2 cM interval between new codominant markers BMp10s174 and BMp10s244. Ten candidate genes were found between markers BMp10s174 and BMp10s244 on chromosome Pv10 and could encode defense response proteins responding to CBB pathogens. Four pairs each of epistatic QTL for CBB resistance were detected at 14 and 21 days after inoculation. Phenotypic variation explained by the epistatic QTL ranged from 7.19% to 12.15% and 7.72% to 8.80% at 14 and 21 days after inoculation, respectively. These results confirmed the importance of epistasis in CBB resistance in common bean. The adjacent markers found may be more efficient for marker assisted selection in common bean breeding for CBB resistance owing to their closer linkage to the target QTL.

关键词： Common bean(Phaseoius vulgaris L.) Common bacterial blight Quantitative trait locus Days after inoculation

Breeding dominant genic male sterility restorer line of Brassica napus L.

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Oil Crop Science 2020年第2期5卷 90-95页

作者： Junying Zhang Jianxia Jiang Liyong Yang Meiyan Jiang Yanli Li Weirong Wang Chaocai Sun jifeng zhu Xirong Zhou Crop Breeding and Cultivation Research Institute Shanghai Academy of Agricultural SciencesShanghaiChina

To facilate breeding process of Brassica napus,a microspore culture and molecular marker-assisted screening combined system were proposed in this *** early flowering stage,F1 offspring of hybridized combination HY15 A×HF06 was used as donor for microspore culture to analyze effects of colchicine concentration on embryogenic and diploid rates of *** with 50 mg/L colchicine resulted in embryogenic rate of3.56 embryos/bud,which was substantially higher than control(0.78 embryos/bud).A total of 1,387 embryos and 862 single plants were obtained after induction *** detection was performed for the regenerated plants by flow *** rates of microspores treated with 50 mg/L and 70 mg/L colchicine were 17.2%and 21.0%respectively,which was significantly higher than control(10.5%).Totally 108 single plants that doubled successfully were randomly selected and screened using molecular marker *** 54 of108 plants generated a 305 bp amplification product,whereas the other 54 plants showed a 398 bp band,thereby satisfying 1:1 separation ratio(x0.052=0.0093).These coincided with field identification *** of this study indicated that homozygous breeding material could be obtained by microspore culture in a short time,thereby remarkably accelerate breeding.

关键词： Brassica napus L. Microspores Double haploid Restorer line Colchicine

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盐酸伐昔洛韦中空栓的制备及质量控制

郑州大学学报（医学版） 2008年第4期43卷 794-795页

作者：朱继峰郑州大学第一附属医院药剂科郑州450052

目的:制备盐酸伐昔洛韦中空栓并建立其质量控制方法。方法:以半合成脂肪酸甘油酯为基质制成中空栓;采用高效液相色谱法测定主药盐酸伐昔洛韦含量,测定波长为254nm,同时进行线性关系、精密度、稳定性、回收率的测定。结果:盐酸伐昔洛韦... 详细信息

目的:制备盐酸伐昔洛韦中空栓并建立其质量控制方法。方法:以半合成脂肪酸甘油酯为基质制成中空栓;采用高效液相色谱法测定主药盐酸伐昔洛韦含量,测定波长为254nm,同时进行线性关系、精密度、稳定性、回收率的测定。结果:盐酸伐昔洛韦检测质量浓度在10.1～50.5mg/L范围内线性关系良好(Y=0.4001X+4.2351,r=0.9998),平均回收率为99.80%(RSD=0.76%)。结论:本方法简便?准确?重现性好,可用于盐酸伐昔洛韦中空栓的质量控制。

关键词：盐酸伐昔洛韦中空栓制备质量控制

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光纤阵列中纤芯间距的检测系统

红外与激光工程 2011年第4期40卷 728-732页

作者：陈立国朱吉锋王明月哈尔滨工业大学机器人研究所黑龙江哈尔滨150001

在光通讯器件中,由光纤阵列和光波导封装而成的光波导分路器芯片,有着广泛的应用。光纤阵列与光波导封装的关键技术在于实现光纤阵列与光波导的低损耗耦合。然而,光纤阵列中纤芯分布的均匀性将会直接影响到耦合的性能,因此,需要对光纤... 详细信息

在光通讯器件中,由光纤阵列和光波导封装而成的光波导分路器芯片,有着广泛的应用。光纤阵列与光波导封装的关键技术在于实现光纤阵列与光波导的低损耗耦合。然而,光纤阵列中纤芯分布的均匀性将会直接影响到耦合的性能,因此,需要对光纤阵列的纤芯分布进行检测,以选择分布均匀的阵列用于和光波导的耦合。系统利用显微视觉和纳米微驱动平台搭建硬件平台,利用最小二乘算法和亚像素图像处理技术提取纤芯的中心,实现纤芯间距的在线检测,纤芯间距的检测精度为50 nm。实验验证了该系统能提高测量的效率和精度,具有很强的实际应用价值。

关键词：光纤阵列显微视觉最小二乘法亚像素

Hepatocyte FoxO1 restrain local NKT cell and hepatic inflammation by control of CD1d expression

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Hepatocyte FoxO1 restrain local NKT cell and hepatic inflamm...

New insight into the origin and evolution of the flora and fauna of Hainan Island, China

第十四届全国免疫学学术大会

作者： Zhigang Lei Sha Zhou Pan Gu Junling Wang jifeng zhu Xiaojun Chen Yalin Li Chuan Su Nanjing Medical University

The liver participates in multiple functions,responsible for metabolism,nutrient storage,detoxification,and production of serum proteins and bile,which are mostly performed by hepatocytes and critical for maintaining physiological *** addition,the liver engages various mechanisms to perpetuate a tolerogenic milieu in response to constant exposure of nonself molecules derived from nutrients or resident bacterial products with inflammatory *** evidence has shown that hepatocytes maintain local immunotolerance through communicating with immune cells in the liver,but the underlying mechanisms remain poorly *** head box O(FoxO) transcription factors and FoxO1 in particular,are critical liver homeostasis regulators to orchestrate gene expression upon cellular *** we aimed to investigate the involvement of hepatocyte FoxO1 in the maintenance of immunological *** generated hepatocyte-specific FoxO1 knockout(FoxO1 △ hepa) mice and found that FoxO1 △ hepa mice had a significantly higher level of serum ALT,as well as local proinflammatory ***,FoxO1 △ hepa mice accumulated more NKT cells and neutrophils in the ***,FoxO1 △ hepa mice with NKT cell depletion had reduced accumulation of neutrophils and attenuated hepatic inflammation,confirming an important role of NKT cells in this *** studies revealed the mechanisms for FoxO1-mediated control of hepatocyte CD1 d expression,which is critical for local NKT cell ***,the protective effect of hepatocyte FoxO1 against liver inflammation was demonstrated in murine model of hepatic ***,our findings uncover a previously unrecognized function of hepatocytes for maintaining liver immunotolerance under both physiological and pathological conditions,that relies on FoxO1-dependent communication between hepatocytes and local immune cells.

关键词： FoxO1 CD1d NKT Neutrophils inflammation

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Science China Earth Sciences 2025年第1期68卷 52-61页

作者： Hua zhu Center for Integrative Conservation Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Yunnan International Joint Laboratory of Southeast Asia Biodiversity Conservation Yunnan Key Laboratory for the Conservation of Tropical Rainforests and Asian Elephants

The origin of the biota of Hainan Island, China, remains a fundamental scientific problem. Comparative studies on the biota, geological tectonics, and paleomagnetism proposed that during the Eocene, Hainan Island connected Vietnam and Guangxi, and then moved to the southeast to reach its present location. This review mainly discusses its biota origin from molecular phylogeny and palaeobotanical research. The formation and evolution of the biological groups of Hainan Island were identified via research on their genomes, phylogeny, and molecular biogeography. This research showed that the genera and species that appeared and diverged early in their evolution(i.e., before the Miocene) can be explained by the land connection between Hainan Island, Vietnam, and Guangxi during the Eocene-Oligocene. According to Southeast Asian tectonic theory, in the Middle Miocene(at about 15 million years ago), Hainan Island had moved to its present position. The population and genetic divergence of species with late divergence times(after 15 Ma) can be reasonably explained by the close proximity(or land connection) between Hainan Island and the Leizhou Peninsula, enabling genetic communication between Hainan Island and the Chinese mainland, but have exceptional cases. Conclusions have been obtained through palaeobotanical studies, showing that in the Eocene-Oligocene period, southwest China and Hainan Island had subtropical vegetation and flora. However, the fossil group in southeast China(in Maoming, near present-day Hainan Island) in the Late Eocene indicated a tropical rainforest vegetation and flora. This suggests that in the Paleogene, Hainan Island may have been located near or on the southwest border of Guangxi and northern Vietnam, rather than in its present location. The floristic division of the flora of Hainan Island basically presents a northeast-southwest inclined distribution pattern. This pattern can be explained by the counterclockwise rotation of Hainan Island during i

关键词： Molecular phylogeny Biogeography Paleobotany Origin of flora and fauna Hainan Island of China

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多层螺旋CT诊断急性胸腹部创伤的准确性及临床价值分析

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影像研究与医学应用 2021年第24期5卷 10-12页

作者：朱纪峰巨野县北城医院放射科山东菏泽274900

目的:研究多层螺旋CT诊断急性胸腹部创伤的准确性及临床价值。方法:选取2018年12月-2020年12月巨野县北城医院接收的急性胸腹部创伤患者58例,分别实施常规诊断和多层螺旋CT诊断,对比两种诊断方式的诊断准确性、误诊率、漏诊率。结果:多... 详细信息

目的:研究多层螺旋CT诊断急性胸腹部创伤的准确性及临床价值。方法:选取2018年12月-2020年12月巨野县北城医院接收的急性胸腹部创伤患者58例,分别实施常规诊断和多层螺旋CT诊断,对比两种诊断方式的诊断准确性、误诊率、漏诊率。结果:多层螺旋CT诊断胸腹部创伤的诊断准确率98.28%显著高于常规诊断的75.86%(P<0.05);多层螺旋CT诊断的误诊率1.72%、漏诊率0.00%均低于常规诊断13.79%、10.34%,差异有统计学意义(P<0.05)。结论:实施多层螺旋CT诊断方法,可直接反映出急性胸腹部创伤患者的病情,为临床诊治夯实基础,降低了误诊率和漏诊率,具有较高的诊断准确性,很大程度上提高了诊断水平,在临床中值得应用和推广。

关键词：多层螺旋CT 急性胸腹部创伤诊断准确性临床价值

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切开法重睑术的研究进展

中华整形外科杂志 2022年第7期38卷 827-833页

作者：朱吉凤刘新昊杨天荣重庆医科大学附属永川医院烧伤整形科重庆402160 重庆医科大学附属第一医院整形烧伤外科重庆400016

切开法是临床上应用最广泛的重睑术式,具体的手术方法有很多,主要的差别是对不同层次进行缝合可产生不同的重睑效果。该文从皮肤、眼轮匝肌、睑板、眶隔、上睑提肌腱膜等组织间所采用的不同缝合方法的角度,对切开法重睑术的研究进展进... 详细信息

切开法是临床上应用最广泛的重睑术式,具体的手术方法有很多,主要的差别是对不同层次进行缝合可产生不同的重睑效果。该文从皮肤、眼轮匝肌、睑板、眶隔、上睑提肌腱膜等组织间所采用的不同缝合方法的角度,对切开法重睑术的研究进展进行综述,并总结了其优缺点,为临床提供参考,以便术者能更好地了解和研究重睑术。

关键词：眼睑成形术切开法上睑提肌腱膜眼轮匝肌

颞骨高分辨CT(HRCT)及三维重建在先天性内耳畸形中的应用价值

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中国医学文摘（耳鼻咽喉科学） 2022年第2期37卷 103-104,110页

作者：朱纪峰巨野县北城医院山东菏泽274900

目的观察在先天性内耳畸形诊断实践中应用颞骨高分辨CT(HRCT)及三维重建的临床价值作用。方法选取36例于2018年1月-2021年1月期间收治的先天性感音神经性耳聋患者,均行颞骨高分辨率CT(HRCT)薄层扫描操作,将所获得原始数据剪薄三维重... 详细信息

目的观察在先天性内耳畸形诊断实践中应用颞骨高分辨CT(HRCT)及三维重建的临床价值作用。方法选取36例于2018年1月-2021年1月期间收治的先天性感音神经性耳聋患者,均行颞骨高分辨率CT(HRCT)薄层扫描操作,将所获得原始数据剪薄三维重建及后处理,深入分析测量获得数据。结果 36例患者中,有25例(43耳)发现存在内耳畸形症状,畸形部位:耳蜗总计14耳,前庭总计7耳,半规管总计5耳,前庭导水管扩大总计12耳,内听道总计5耳。结论颞骨HRCT及三维重建图像后处理技术可将患者内耳结构及伴发畸形进行充分清晰展现,为临床诊治先天性内耳畸形提供科学参考依据。

关键词：颞骨高分辨CT 三维重建先天性内耳畸形临床价值