Breeding dominant genic male sterility restorer line of Brassica napus L.

作     者：Junying Zhang Jianxia Jiang Liyong Yang Meiyan Jiang Yanli Li Weirong Wang Chaocai Sun Jifeng Zhu Xirong Zhou Junying Zhang;Jianxia Jiang;Liyong Yang;Meiyan Jiang;Yanli Li;Weirong Wang;Chaocai Sun;Jifeng Zhu;Xirong Zhou

作者机构：Crop Breeding and Cultivation Research InstituteShanghai Academy of Agricultural SciencesShanghaiChina 

出 版 物：《Oil Crop Science》 (中国油料作物学报(英文版）)

年 卷 期：2020年第5卷第2期

页      面：90-95页

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by the Youth Talent Development Plan of Shanghai Municipal Agricultural System,China(No.20180103) the National Key Research and Development Program of China(No.2018YFD0100602) the Youth Talent Running Plan of Shanghai Academy of Agricultural Sciences,China(No.2017020) 

主　　题：Brassica napus L. Microspores Double haploid Restorer line Colchicine 

摘      要：To facilate breeding process of Brassica napus,a microspore culture and molecular marker-assisted screening combined system were proposed in this *** early flowering stage,F1 offspring of hybridized combination HY15 A×HF06 was used as donor for microspore culture to analyze effects of colchicine concentration on embryogenic and diploid rates of *** with 50 mg/L colchicine resulted in embryogenic rate of3.56 embryos/bud,which was substantially higher than control(0.78 embryos/bud).A total of 1,387 embryos and 862 single plants were obtained after induction *** detection was performed for the regenerated plants by flow *** rates of microspores treated with 50 mg/L and 70 mg/L colchicine were 17.2%and 21.0%respectively,which was significantly higher than control(10.5%).Totally 108 single plants that doubled successfully were randomly selected and screened using molecular marker *** 54 of108 plants generated a 305 bp amplification product,whereas the other 54 plants showed a 398 bp band,thereby satisfying 1:1 separation ratio(x0.052=0.0093).These coincided with field identification *** of this study indicated that homozygous breeding material could be obtained by microspore culture in a short time,thereby remarkably accelerate breeding.