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Chinese Chemical Letters 2021年第11期32卷 3514-3517页

作者： Qing Yu Yunyun Liu jie-Ping wan College of Chemistry and Chemical Engineering Jiangxi Normal UniversityNanchang 330022China

The perfluoroalkylsulfonylation(CF_(3)SO_(2),C_(2)F_(5)SO_(2)and CHF_(2)SO_(2))in the enaminone C-H bonds has been developed simply via the promotion of molecular iodine by using stable and cheap sodium perfluoroalkyl sulfinates as coupling partners.The stereoselective synthesis of E-configuratedα-perfluoroalkylsulfonyl enaminones has been realized via unprecedented C-H bond elaboration and C=C double bond configuration inversion by free radical process.

关键词： C-H Functionalization Enaminone Perfluoroalkyl sulfonylation Metal-free Configuration inversion

Conditional Genome Editing in the Mammalian Brain Using CRISPR-Cas9

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Neuroscience Bulletin 2021年第3期37卷 423-426页

作者： Haojie Sun jie Zheng Ming Yi You wan Neuroscience Research Institute Department of NeurobiologySchool of Basic Medical SciencesPeking UniversityBeijing 100083China Key Laboratory for Neuroscience Ministry of Education/National Health Commission of ChinaPeking UniversityBeijing 100083China Department of Pathophysiology Huazhong University of Science and TechnologyWuhan 430030China

Neuronal ensembles with distinct morphological,biochemical,and functional identities are organized into complex circuits in the mammalian brain,and malfunction of specific neuronal types in different networks contributes to diverse pathological symptoms.Taking memory as an example,specific memories are held in a subset of neurons,referred to as engram cells.

关键词： Mammalian networks organized

Lifetime estimation of IGBT module using square-wave loss discretization and power cycling test

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Nuclear Science and Techniques 2022年第10期33卷 126-138页

作者： Jie Wang Da-Qing Gao wan-Zeng Shen Hong-Bin Yan Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China

The insulated gate bipolar transistor(IGBT)module is one of the most age-affected components in the switch power supply, and its reliability prediction is conducive to timely troubleshooting and reduction in safety risks and unnecessary costs. The pulsed current pattern of the accelerator power supply is different from other converter applications;therefore, this study proposed a lifetime estimation method for IGBT modules in pulsed power supplies for accelerator magnets. The proposed methodology was based on junction temperature calculations using square-wave loss discretization and thermal modeling.Comparison results showed that the junction temperature error between the simulation and IR measurements was less than 3%. An AC power cycling test under real pulsed power supply applications was performed via offline wearout monitoring of the tested power IGBT module. After combining the IGBT4 PC curve and fitting the test results,a simple corrected lifetime model was developed to quantitatively evaluate the lifetime of the IGBT module,which can be employed for the accelerator pulsed power supply in engineering. This method can be applied to other IGBT modules and pulsed power supplies.

关键词： IGBT module Junction temperature Power cycling test Lifetime prediction Power loss discretization

OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield

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The Crop Journal 2022年第2期10卷 397-406页

作者： Jie Wang Renjing wan Haipeng Nie Shaowu Xue Zhongming Fang Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Agricultural SciencesGuizhou UniversityGuiyang 550025GuizhouChina Center of Applied Biotechnology Wuhan University of BioengineeringWuhan 430415HubeiChina College of Life Science and Technology Huazhong Agricultural UniversityWuhan 430070HubeiChina

Rice has a large number of nitrate or peptide transporter family(NPF) genes, but the effects of most members on rice growth and development are unknown. We report that Os NPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of Os NPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica cultivars.Os NPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In c RNA-injected Xenopus laevis oocytes, Os NPF5.16 transport of nitrate at high nitrate concentration depended on p H. Overexpression of Os NPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of Os NPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of Os NPF5.16 expression showed the opposite effects. Regulating Os NPF5.16 expression has potential for improving rice grain yield.

关键词： Rice OsNPF5.16 Nitrate transporter Natural variation Growth Grain yield

An overview of flow field computational methods for hydrodynamic noise prediction

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Journal of Hydrodynamics 2022年第6期34卷 994-1005页

作者： Wei-wen Zhao Zhi Pan Lian-jie Yu De-cheng wan Computational Marine Hydrodynamics Lab(CMHL) School of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Wuhan Second Ship Design and Research Institute Wuhan430205China

Recently,the hydrodynamic noise is becoming a research hotspot because it not only affects the concealment and comfort of ships,but also affects the living condition of underwater mammals.Accurate prediction of hydrodynamic noise requires that the detailed flow field has been simulated temporally and spatially with high fidelity method.In this paper,we introduce the current issues and challenges for the prediction of hydrodynamic noise,and provide an overview to several detailed flow field simulation methods which aim to resolve these issues.The overview could point the future directions for hydrodynamic noise prediction.

关键词： Hydrodynamic noise turbulent fluctuation wall-bounded flow two-phase flow noise reduction

Gut microbiota bridges the iron homeostasis and host health

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中国科学:生命科学英文版 2023年第9期66卷 1952-1975页

作者： Lanling Xiao Rui Tang Jie Wang Dan wan Yulong Yin Liwei Xie State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationGuangdong Open Laboratory of Applied MicrobiologyInstitute of MicrobiologyGuangdong Academy of SciencesGuangzhou 510070China Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 101408China Department of Psychiatry The First Affiliated Hospital of Jinan UniversityGuangzhou 510000China Department of Endocrinology and Metabolism Zhujiang HospitalSouthern Medical UniversityGuangzhou 510280China School of Public Health Xinxiang Medical UniversityXinxiang 453003China Department of Stomatology Shunde HospitalSouthern Medical University(The First People’s Hospital of ShundeFoshan)Foshan 528308China Department of Life Sciences Imperial College LondonLondon SW72AZUnited Kingdom

The gut microbiota acts as a symbiotic microecosystem that plays an indispensable role in the regulation of a number of metabolic processes in the host by secreting secondary metabolites and impacting the physiology and pathophysiology of numerous organs and tissues through the circulatory system. This relationship, referred to as the “gut-X axis”, is associated with the development and progression of disorders, including obesity, fatty liver and Parkinson's disease. Given its importance, the gut flora is a vital research area for the understanding and development of the novel therapeutic approaches for multiple disorders. Iron is a common but necessary element required by both mammals and bacteria. As a result, iron metabolism is closely intertwined with the gut microbiota. The host's iron homeostasis affects the composition of the gut microbiota and the interaction between host and gut microbiota through various mechanisms such as nutrient homeostasis, intestinal peaceability,gut immunity, and oxidative stress. Therefore, understanding the relationship between gut microbes and host iron metabolism is not only of enormous significance to host health but also may offer preventative and therapeutic approaches for a number of disorders that impact both parties. In this review, we delve into the connection between the dysregulation of iron metabolism and dysbiosis of gut microbiota, and how it contributes to the onset and progression of metabolic and chronic diseases.

关键词： gut microbiota iron metabolic syndrome diseases

Investigating the impacts of spatial-temporal variation features of air density on assessing wind power generation and its fluctuation in China

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Science China(Technological Sciences) 2023年第6期66卷 1797-1814页

作者： REN GuoRui wanG Wei wan jie HONG Feng YANG Ke YU DaRen School of Control and Computer Engineering North China Electric Power UniversityBeijing 102206China School of Electrical Engineering and Automation Harbin Institute of TechnologyHarbin 150001China Xi’an Thermal Power Research Institute Co. Ltd.Xi’an 710054China School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China

Air density plays an important role in assessing wind resource.Air density significantly fluctuates both spatially and temporally.But literature typically used standard air density or local annual average air density to assess wind resource.The present study investigates the estimation errors of the potential and fluctuation of wind resource caused by neglecting the spatial-temporal variation features of air density in China.The air density at 100 m height is accurately calculated by using air temperature,pressure,and humidity.The spatial-temporal variation features of air density are firstly analyzed.Then the wind power generation is modeled based on a 1.5 MW wind turbine model by using the actual air density,standard air densityρst,and local annual average air densityρsite,respectively.Usingρstoverestimates the annual wind energy production(AEP)in 93.6%of the study area.Humidity significantly affects AEP in central and southern China areas.In more than 75%of the study area,the winter to summer differences in AEP are underestimated,but the intra-day peak-valley differences and fluctuation rate of wind power are overestimated.Usingρsitesignificantly reduces the estimation error in AEP.But AEP is still overestimated(0-8.6%)in summer and underestimated(0-11.2%)in winter.Except for southwest China,it is hard to reduce the estimation errors of winter to summer differences in AEP by usingρsite.Usingρsitedistinctly reduces the estimation errors of intra-day peak-valley differences and fluctuation rate of wind power,but these estimation errors cannot be ignored as well.The impacts of air density on assessing wind resource are almost independent of the wind turbine types.

关键词： air density spatial-temporal variation power curve wind resource assessment fluctuation ERA-5

Reproductive polyphenism and its advantages in aphids:Switching between sexual and asexual reproduction

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Journal of Integrative Agriculture 2020年第6期19卷 1447-1457页

作者： YAN Shuo wanG wan-xing SHEN jie Department of Entomology/MOA Key Lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijing 100193P.R.China Key Lab of Biology and Genetic Improvement of Root and Tuber Crops Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Reproductive polyphenism,which allows one genotype to produce sexual and asexual morphs,is an extreme case of phenotypic plasticity and is commonly observed in aphids.Aphids are typical species that switch these reproductive modes,and the pathway orientation is triggered by the environmental conditions(mainly photoperiod and temperature).The typical arm ual life of aphids in eludes a successi on of parthenogenetic gen erations duri ng the spri ng and summer and a single sexual generation in autumn.In this review,we describe how the environmental cues orientate the reproductive mode of aphids from photoperiodic perception to endocrine regulation,and how juvenile hormones may act on the target cells(oocytes)to initiate the gametogenesis and embryogenesis in sexual and asexual reproduction.We also discuss the paradox of sex,especially the advantages of sexual reproduction in aphids.With the recent development of genomic resources in aphids,many potential genes involved in the reproductive polyphenism will enter the public's awareness.In particular,we describe a novel RNAi method in aphids,which may provide a molecular technique for determining the developmental fate and multiple reproductive strategies.

关键词： aphids parthenogenesis embryogenesis gametogenesis photoperiodism

Intracellular and extracellular enzymatic responsive micelle for intelligent therapy of cancer

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Nano Research 2023年第2期16卷 2851-2858页

作者： Dong wan Qinan Zhu Jianxin Zhang Xi Chen Fangzhou Li Yi Liu jie Pan School of Chemical Engineering and Technology Tiangong UniversityTianjin 300387China School of Chemistry Tiangong UniversityTianjin 300387China CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China

Recently,the incidence of cancer keeps increasing,seriously endangers human health,and has evolved into the main culprit of human death.Conventional chemotherapeutic drugs,such as paclitaxel and doxorubicin(DOX),have some disadvantages,including low therapeutic effect,poor water solubility,high toxic side effects,short blood circulation time in the body,and so on.To improve the anti-tumor effect of the drug in vivo and reduce its side effects on the body,researchers have designed and developed a variety of responsive nanocarriers.In this work,we synthesized D-α-tocopherol polyethylene glycol 3350 succinate(TPGS3350)-Gly-Pro-Leu-Gly-Val-Arg(GPLGVR)-DOX(TPD)prodrugs in response to extracellular enzymes of matrix metalloproteinase(MMP-9)in the tumor microenvironment and FA-Asp-Glu-Val-Asp(DEVD)-DOX(FPD)prodrugs responsive to intracellular enzymes of caspase-3.Then,intracellular and extracellular enzyme-responsive TPD&FPD micelles with DOX(TPD&FPD&D)were successfully prepared through dialysis method.The outer layer of TPGS3350 can prolong the blood circulation time of micelles in vivo,followed by accumulation of micelles at tumor tissue through enhanced permeability and retention(EPR)effect.The peptide of GPLGVR can be cleaved by MMP-9 enzymes to remove the outer layer of TPGS3350,exposing the targeting molecule of folate,and then the micelles are engulfed by tumor cells through folate receptor-mediated endocytosis.After entering the tumor cells,the free DOX loaded in the micelles is released,which induces tumor cell apoptosis to activate caspase-3 in the cells,cutting the peptide DEVD to accelerate the intracellular release of the DOX,which further enhances cytotoxicity to improve antitumor effect.

关键词： cancer micelle peptide enzyme-responsive folate

Light-activated arginine-rich peptide-modified nanoparticles for deep-penetrating chemo-photo-immunotherapy of solid tumor

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Nano Research 2023年第7期16卷 9804-9814页

作者： Yonghua Gong Jinyang Zhang Yan Lu Dong wan jie Pan Guilei Ma Key Laboratory of Biomaterials and Nanotechnology for Cancer Immunotherapy The Tianjin Key Laboratory of BiomaterialsInstitute of Biomedical EngineeringPeking Union Medical College&Chinese Academy of Medical SciencesTianjin 300192China School of Environmental and Chemical Engineering Tiangong UniversityTianjin 300387China

Poor permeation of drugs and“immune-cold”tumor microenvironment in solid tumors are the two major challenges which lead to the inefficient therapeutic efficacy for cancer treatment.Here,light-activated penetrable nanoparticles(PEGVAL&DOX&ICG@RNPs)for co-delivery of the chemotherapeutic drug doxorubicin(DOX),the photosensitizer agent indocyanine green(ICG),and the angiotensin II receptor blockers valsartan(VAL)were developed to achieve deep drug penetration and synergistic photo-chemo-immunotherapy of solid tumor.Studies showed that under the first-wave of laser irradiation,the polyethylene glycol(PEG)hydrophilic layer as an“inert”surface could detach from the nanoparticles,release VAL and expose the arginine-rich peptide modified-cores that can facilitate deep drug penetration via a transcytosis pathway.When exposed to the second-wave of laser irradiation,the synergistic chemo-photo-immunotherapy can be achieved.As expected,in 4T1 tumorbearing mice,PEG-VAL&DOX&ICG@RNPs treatment could effectively inhibit the growth of tumors,down-regulateα-smooth muscle actin expression level of cancer-associated fibroblasts cells in tumors,induce dendritic cells(DCs)maturation,and promote intratumoral infiltration of cytotoxic T lymphocytes.Moreover,combination therapy by PEG-VAL&DOX&ICG@RNPs and anti-PD-1 monoclonal antibody can elicit memory T cell response for preventing tumor recurrence and metastasis in vivo.This work provides a promising delivery strategy to overcome the current limitations of nanomedicine for achieving more effective therapeutic index of“immune-cold”solid tumor treatment.

关键词： light-activated nanoparticles transcytosis deep penetration chemo-photo-immunotherapy solid tumor