OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield

作     者：Jie Wang Renjing Wan Haipeng Nie Shaowu Xue Zhongming Fang Jie Wang;Renjing Wan;Haipeng Nie;Shaowu Xue;Zhongming Fang

作者机构：Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)College of Agricultural SciencesGuizhou UniversityGuiyang 550025GuizhouChina Center of Applied BiotechnologyWuhan University of BioengineeringWuhan 430415HubeiChina College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第2期

页      面：397-406页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by the National Key Research and Development Program(2016YFD0100700) the Wuhan Science and Technology Project(2020020601012259) Hubei Natural Science Foundation(2020CFB117) the National Natural Science Foundation of China(31301250) the Talent Project from Guizhou Education Department(Qian jiao he KY zi(2021)024) the Key Cultivation Project of Guizhou University(201903) the Talent Project from Thousands of Innovative and Entrepreneurial in Guizhou Province。 

主　　题：Rice OsNPF5.16 Nitrate transporter Natural variation Growth Grain yield 

摘      要：Rice has a large number of nitrate or peptide transporter family(NPF) genes, but the effects of most members on rice growth and development are unknown. We report that Os NPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of Os NPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica cultivars.Os NPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In c RNA-injected Xenopus laevis oocytes, Os NPF5.16 transport of nitrate at high nitrate concentration depended on p H. Overexpression of Os NPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of Os NPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of Os NPF5.16 expression showed the opposite effects. Regulating Os NPF5.16 expression has potential for improving rice grain yield.