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Monitoring and identification of spoilage-related microorganisms in braised chicken with modified atmosphere packaging during refrigerated storage

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Food Science and Human Wellness 2023年第1期12卷 28-34页

作者： Yang Lei Yali Zhang Yiqun Cheng jichao huang Ming huang Key Laboratory of Meat Processing and Quality Control Ministry of EducationCollege of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China National R&D Center for Poultry Processing Jiangsu Research Center for Livestock and Poultry Products Processing Engineering TechnologyNanjing Huangjiaoshou Food Science and Technology Co.Ltd.Nanjing 211225China College of Environmental Science&Engineering Institute of Functional FoodAnhui Normal UniversityWuhu 241000China College of Engineering Nanjing Agricultural UniversityNanjing 210031China

This study mainly monitored the dominant bacterial populations and identified the spoilage-related microorganisms of braised chicken meat stored under different CO_(2)-modified atmosphere packaging(MAP)during refrigerated storage using a culture-dependent method and 16S rDNA identification.The quality changes and shelf life of the meat were also measured.The growth rate of total viable count(TVC)in braised chicken was slower with an increase of CO_(2) content in MAP,which also occurred in the remaining bacterial species monitored(lactic acid bacteria,Pseudomonas spp.,Brochothrix thermosphacta).The MAP exerted beneficial effects on the quality of braised chicken,as demonstrated by retarding the production of total volatile basic nitrogen(TVB-N)and delaying lipid oxidation(TBARS test).A total of 14 isolates were identified from braised chickens with different packaging at the end of storage,these included P.fragi(6 isolates),P.psychrophila(2 isolates),Enterococcus faecalis(3 isolates),B.thermosphacta(2 isolates),Staphylococcus equorum(1 isolate).

关键词： Braised chicken Modified atmosphere packaging(MAP) 16S rDNA identification Shelf-life

In-package cold plasma treatment of braised chicken: voltage effect

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Food Science and Human Wellness 2022年第4期11卷 845-853页

作者： Yali Zhang Yang Lei Suhong huang Xiaoli Dong jichao huang Ming huang Nanjing Innovation Center of Meat Products Processing Jiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety Controland College of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China College of Engineering Nanjing Agricultural UniversityNanjing 210095China

This study investigated the effect of different dielectric barrier discharge cold plasma(DBD-CP) treatment voltages on the microbiological growth and quality characteristics of braised chicken during storage at(4 ± 1) ℃. Argon-packed samples were subjected to DBD-CP treatment for 3 min at voltages of 50, 60 and 70 k V. As a result, there was no significant(P > 0.05) difference in L*, a*, b* and p H of braised chicken between control and treatment groups at the same storage time. However, the development of lipid oxidation in DBD-CP treatment samples was slower than that in air-packed samples. Compared to air-packed samples, the reduction of total viable count, yeasts and molds, lactic acid bacteria and Pseudomonads spp. in 70 k V treated samples on day 15 were 3.21, 2.41, 2.44 and 1.96 lg(CFU/g), respectively. The results indicate that DBD-CP treatment could extend the shelf life of braised chicken without negative impacts on quality characteristics.

关键词： In-package cold plasma Braised chicken Shelf-life Quality

The Grid Search Algorithm of Tectonic Stress Tensor Based on Focal Mechanism Data and Its Application in the Boundary Zone of China, Vietnam and Laos

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Journal of Earth Science 2016年第5期27卷 777-785页

作者： Yongge Wan Shuzhong Sheng jichao huang Xiang Li Xin Chen Institute of Disaster Prevention Sanhe 065201 China Earthquake Administration of the Guangxi Autonomous Region Nanning 530022 China

Stress field plays a key role in geodynamics. In this study, an algorithm to determine the stress tensor and its confidence range from focal mechanism data by using grid search method was proposed. The experiment uses artificial focal mechanism data which were generated by extensional, compression and strike-slip stress regime and different level of noise, shows that the precision of the estimated stress tensor based on this algorithm is greatly improved compared with traditional algorithms. This algorithm has three advantages：（1） The global optimal solution of the stress tensor is determined by fine grid search of 1o×1o×1o×0.01 and local minimum value is avoided; （2） precision of focal mechanism data can be considered, i.e., different weight of the focal mechanism data contributes differently to the process of determining stress tensor; （3） the confidence range of the determined stress tensor can be obtained by using F-test. We apply this algorithm in the boundary zone of China, Vietnam and Laos, and obtain the stress field with SSE-NNW compressive stress direction and NEE-SWW extensional stress direction. The stress ratio is 0.6, which shows that the eigen values of the stress tensor are nearly in arithmetic sequence. The stress field in this region is consistent with the left-lateral strike slip of the Dienbien-Lauangphrabang arc fault. The result will be helpful in studying the geological dynamic process in this region.

关键词： stress tensor grid search focal mechanism uncertainty.

冻藏时间对不同热处理方式鸭胸肉中羧甲基赖氨酸形成的影响

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南京农业大学学报 2024年第2期47卷 359-368页

作者：王倩楠朱宗帅黄天然黄继超黄明南京农业大学食品科技学院江苏南京210095 南京黄教授食品科技有限公司/江苏省畜禽产品加工工程技术研究中心/国家禽肉加工技术研发专业中心江苏南京211225 南京农业大学工学院江苏南京210031

[目的]本文旨在阐明不同热处理方式(烤制、炸制、煮制、煎制、高压炖煮)鸭胸肉经不同时间冻藏(-20℃,分别贮藏0、10、20、30、40 d)后,氧化(脂质和蛋白质氧化)与蛋白质结构改变对羧甲基赖氨酸(CML)形成的影响。[方法]采用酶联免疫、多... 详细信息

[目的]本文旨在阐明不同热处理方式(烤制、炸制、煮制、煎制、高压炖煮)鸭胸肉经不同时间冻藏(-20℃,分别贮藏0、10、20、30、40 d)后,氧化(脂质和蛋白质氧化)与蛋白质结构改变对羧甲基赖氨酸(CML)形成的影响。[方法]采用酶联免疫、多光谱分析、相关分析等技术方法测定经热/非热处理鸭胸肉冻藏期间的脂质氧化、游离氨基、表面疏水性、内源性色氨酸和蛋白质氧化等指标,探究冷冻贮藏对鸭肉肌原纤维蛋白(MP)结构修饰及CML形成的关联机制。[结果]在40 d、-20℃的冻藏条件下,各组冻藏过程中游离态和结合态CML生成量不同,主要受到蛋白质氧化聚集和脂质氧化的影响。在冻藏过程中CML生成量主要与羰基含量、总巯基含量、脂质氧化显著相关(P<0.05)。与其他组相比,高压炖煮组在冻藏期间CML含量最高,炸制与高压炖煮促进鸭胸肉蛋白质羰基化并导致其结构改变,烤制和煮制组促进脂质氧化进程。[结论]热加工肉经冻藏后促进蛋白质分子降解、二次聚集、开链等行为,从而影响CML生成量。

关键词：鸭胸肉冻藏羧甲基赖氨酸氧化结构修饰

Co-seismic fault geometry and slip distribution of the 26 December 2004, giant Sumatra–Andaman earthquake constrained by GPS, coral reef, and remote sensing data

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Earthquake Science 2015年第3期28卷 187-195页

作者： Yongge Wan Zheng-kang Shen Min Wang Yuehua Zeng jichao huang Xiang Li Huawei Cui Xiwei Gao Institute of Disaster-Prevention Department of Earth and Space Sciences University of California Institute of Geology China Earthquake Administration U.S.Geology Survey

We analyze co-seismic displacement field of the 26 December 2004, giant Sumatra–Andaman earthquake derived from Global Position System observations,geological vertical measurement of coral head, and pivot line observed through remote sensing. Using the co-seismic displacement field and AK135 spherical layered Earth model, we invert co-seismic slip distribution along the seismic fault. We also search the best fault geometry model to fit the observed data. Assuming that the dip angle linearly increases in downward direction, the postfit residual variation of the inversed geometry model with dip angles linearly changing along fault strike are plotted. The geometry model with local minimum misfits is the one with dip angle linearly increasing along strike from 4.3oin top southernmost patch to 4.5oin top northernmost path and dip angle linearly increased. By using the fault shape and geodetic co-seismic data, we estimate the slip distribution on the curved fault. Our result shows that the earthquake ruptured ＊200-km width down to a depth of about 60 km.0.5–12.5 m of thrust slip is resolved with the largest slip centered around the central section of the rupture zone78N–108N in latitude. The estimated seismic moment is8.2 9 1022 N m, which is larger than estimation from the centroid moment magnitude（4.0 9 1022 N m）, and smaller than estimation from normal-mode oscillation data modeling（1.0 9 1023 N m）.

关键词： Sumatra–Andaman earthquake Fault geometry Co-seismic slip distribution Geodetic data

氧化石墨烯及其复合材料去除水体抗生素的研究进展

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生态环境学报 2024年第1期33卷 144-155页

作者：李丹怡黄显婷李继超李颖洁闫家普林慰北京师范大学自然科学高等研究院广东珠海519087 中国水产科学研究院南海水产研究所/农业农村部水产品加工重点实验室广东广州510300 北京师范大学珠海分校不动产学院广东珠海519087 聊城市政务服务综合受理中心山东聊城252000

由于具有优异的抗菌性,抗生素被广泛应用于医学领域和养殖行业。然而,近年来对抗生素大规模的使用和排放,在自然界造成了大量的累积,引发了一系列水环境污染问题,严重威胁生态系统安全和人类健康。因此,研发可去除水中过量抗生素的材料... 详细信息

由于具有优异的抗菌性,抗生素被广泛应用于医学领域和养殖行业。然而,近年来对抗生素大规模的使用和排放,在自然界造成了大量的累积,引发了一系列水环境污染问题,严重威胁生态系统安全和人类健康。因此,研发可去除水中过量抗生素的材料已成为当前环境领域的重要研究方向之一。氧化石墨烯(graphene oxide,GO)以其高比表面积、良好亲水性、耐酸碱性、制备简便、成本低廉等优势,成为了一种新兴的抗生素去除材料。该文首先概述了现有的GO及其复合材料分类制备方法,包括空间改造、共价键修饰和金属氧化物改性等。其次,综述了近年来GO及其复合材料在抗生素去除领域的应用、影响因素及作用机制。结果表明,基于吸附原理的GO复合材料吸附去除抗生素效果主要受溶液p H、共存离子、吸附剂投加量、抗生素类别及浓度等多种因素的影响,吸附机制主要包括静电相互作用、π-π相互作用、阳离子-π键作用和氢键作用等,通过引入元素、金属和有机化合物等手段可有效提高GO材料的吸附容量;基于光催化降解原理的抗生素去除过程中,光照条件是最重要影响因素,而其中关键活性物质包括光生空穴、羟基自由基和超氧自由基等,掺杂金属纳米粒子与半导体纳米材料等能够优化其光催化性能。最后,通过对GO及其复合材料制备过程、去除机理和应用场景的综合分析,指出其普适性不高、实际应用不足和环境毒性不明等现存问题,并提出未来应加强材料研发、产业应用和毒性效应评估等方面的研究展望。

关键词：氧化石墨烯复合材料抗生素吸附光催化降解去除机理

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焦作煤田地下水环境影响研究

能源与环保 2024年第5期46卷 64-70,77页

作者：黄继超邓晓颖孟飞张公河南省地质局生态环境地质服务中心河南郑州450053

地下水环境问题会导致可利用的地下水资源减少、地下水环境的恶化,从而对人类生活和环境造成严重影响。采用资料收集、开展水文地质调查、评价分析等方法,查明研究区地下水水文地质条件变化特征和矿区水文地质特征,分析地下水环境产生... 详细信息

地下水环境问题会导致可利用的地下水资源减少、地下水环境的恶化,从而对人类生活和环境造成严重影响。采用资料收集、开展水文地质调查、评价分析等方法,查明研究区地下水水文地质条件变化特征和矿区水文地质特征,分析地下水环境产生影响的各种因素。研究表明,由于煤矿开采导致地下水水位下降,已造成局部地区地下水径流场发生改变;水位下降导致河渠及泉水排泄地下水的形式消失;目前地下水位降落漏斗仍在扩大,郇封漏斗与温孟漏斗有相互连通而成为一个大复漏斗的趋势;煤矿开采致使地下水水质污染有向“深度”扩展的趋势;矿区排水造成疏干区扩大会进一步加剧诱发地面沉陷(塌陷)问题的产生。研究结果为保障水资源的可持续利用、促进经济、社会和环境的协调发展提供了科学依据。

关键词：地下水环境煤矿开采防治措施焦作市

Glycolysis and acute lung injury:A review

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Asian Pacific Journal of Tropical Medicine 2023年第11期16卷 490-497页

作者： Yang Yi Jun Chen Nan Li Yue huang jichao Peng Xiaoran Liu College of Emergency Trauma Hainan Medical UniversityHaikouChina Key Laboratory of Hainan Trauma and Disaster Rescue the First Affiliated Hospital of Hainan Medical UniversityHaikouChina Key Laboratory of Emergency and Trauma Ministry of Education Hainan Medical UniversityHaikouChina Danzhou People’s Hospital DanzhouChina

Acute lung injury is featured as diffuse pulmonary edema and persistent hypoxemia caused by lung or systemic injury.It is believed that these pathological changes are associated with damage to the alveolar epithelium and vascular endothelium,recruitment of inflammatory cells,and inflammatory factor storms.In recent years,the metabolic reprogramming of lung parenchymal cells and immune cells,particularly alterations in glycolysis,has been found to occur in acute lung injury.Inhibition of glycolysis can reduce the severity of acute lung injury.Thus,this review focuses on the interconnection between acute lung injury and glycolysis and the mechanisms of interaction,which may bring hope for the treatment of acute lung injury.

关键词： Acute lung injury Glycolysis Hypoxia-inducible factor 1 Endothelium Macrophages

二十二碳六烯酸对蛛网膜下腔出血大鼠海马神经细胞凋亡及JNK蛋白的影响

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中华行为医学与脑科学杂志 2024年第5期33卷 439-444页

作者：黄啸元麦麦提依明·托合提乌拉别克·毛力提张诚吴永刚王继超新疆维吾尔自治区人民医院神经外科乌鲁木齐830001

目的:探讨二十二碳六烯酸(docosahexaenoic acid,DHA)对蛛网膜下腔出血(subarachnoid hemorrhage,SAH)大鼠神经细胞凋亡及c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)蛋白的影响。方法:48只SPF级雄性SD大鼠按照随机数字表法分为对照组、假手术组、模型组和DHA干预组,每组12只。模型组和DHA组大鼠通过视交叉池内注射自体血(0.3 mL)法建立SAH模型,DHA干预组大鼠在SAH模型建立后3 h给予腹腔注射DHA(35 mg/kg),假手术组大鼠在视交叉池内注射0.3 mL 0.9%氯化钠溶液,对照组大鼠正常饲养。各组大鼠均在24 h后进行神经行为功能评估;利用TUNEL染色法观察大鼠神经细胞凋亡情况,Western blot观察磷酸化JNK(phosphorylated JNK,p-JNK)以及凋亡相关蛋白Bax、Bcl-2的表达情况。使用GraphPad Prism 7.0软件进行统计分析,多组间比较采用单因素方差分析,进一步两两比较采用Tukey检验。结果:(1)4组大鼠的神经行为学评分差异有统计学意义(F=103.60,P0.05)。4组大鼠海马组织Bcl-2、p-JNK蛋白表达水平及Bax/Bcl-2比值均差异有统计学意义(F=8.48,5.69,5.39,均P0.05);模型组大鼠p-JNK蛋白水平(1.93±0.25)及Bax/Bcl-2比值(2.05±0.86)均高于假手术组[(1.42±0.33),(1.05±0.26)](均P<0.05),Bcl-2蛋白水平(1.04±0.23)低于假手术组(1.61±0.16)(P<0.05);DHA干预组大鼠p-JNK蛋白水平(1.43±0.33)及Bax/Bcl-2比值(1.19±0.30)均低于模型组(均P<0.05),Bcl-2蛋白水平(1.42±0.28)高于模型组(P<0.05)。结论:DHA能够抑制p-JNK的激活,减轻蛛网膜下腔出血后的神经细胞凋亡,并改善SAH后大鼠的神经功能。

关键词：二十二碳六烯酸蛛网膜下腔出血神经细胞凋亡 c-Jun氨基末端激酶大鼠