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Multi-omics Data Reveal the Effect of Sodium Butyrate on Gene Expression and Protein Modification in Streptomyces

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Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1149-1162页

作者： jiazhen zheng Yue Li Ning Liu Jihui Zhang Shuangjiang Liu Huarong Tan State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Microbial Biotechnology Shandong UniversityQingdao 266237China

Streptomycetes possess numerous gene clusters and the potential to produce a large amount of natural *** deacetylase(HDAC)inhibitors play an important role in the regulation of histone modifications in fungi,but their roles in prokaryotes remain poorly ***,we investigated the global effects of the HDAC inhibitor,sodium butyrate(SB),on marine-derived Streptomyces olivaceus FXJ 8.021,particularly focusing on the activation of secondary metabolite *** antiSMASH analysis revealed 33 secondary metabolite biosynthetic gene clusters(BGCs)in strain FXJ 8.021,among which the silent lobophorin BGC was activated by *** data showed that the expression of genes involved in lobophorin biosynthesis(ge00097–ge00139)and CoA-ester formation(e.g.,ge02824),as well as the glycolysis/gluconeogenesis pathway(e.g.,ge01661),was significantly up-regulated in the presence of *** CoA-ester analysis confirmed that SB triggered the biosynthesis of CoA-ester,thereby increasing the precursor supply for lobophorin *** acetylomic analysis revealed that the acetylation levels on 218 sites of 190 proteins were up-regulated and those on 411 sites of 310 proteins were *** acetylated proteins were particularly enriched in transcriptional and translational machinery components(e.g.,elongation factor GE04399),and their correlations with the proteins involved in lobophorin biosynthesis were established by protein–protein interaction network analysis,suggesting that SB might function via a complex hierarchical.

关键词： Silent gene cluster Streptomyces Sodium butyrate Protein modification Acetylome

Important role of a LAL regulator Sta R in the staurosporine biosynthesis and high-production of Streptomyces fradiae CGMCC 4.576

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Science China(Life Sciences) 2019年第12期62卷 1638-1654页

作者： Hanye Guan Yue Li jiazhen zheng Ning Liu Jihui Zhang Huarong Tan State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Staurosporine, belonging to indolocarbazole compounds, is regarded as an excellent lead compound for synthesizing antitumor agents as a potent inhibitor against various protein kinases. In this study, two separate clusters(cluster A and cluster B),corresponding to biosyntheses of K-252 c(staurosporine aglycone) and sugar moiety, were identified in Streptomyces fradiae CGMCC 4.576 and heterologously expressed in Streptomyces coelicolor M1146 separately or together. Sta R, a cluster-situated LAL family regulator, activates staurosporine biosynthesis by binding to the promoter regions of sta O-sta C and sta G-sta N. The conserved sequences GGGGG and GCGCG were found through gradually truncating promoters of sta O and sta G, and further determined by mutational experiments. Overexpression of sta R with the supplementation of 0.01 g L^–1 Fe SO4 increased staurosporine production to 5.2-fold compared with that of the parental strain Streptomyces fradiae CGMCC 4.576 in GYM medium. Our results provided an approach for improvement of staurosporine production mediated by a positive regulator and established the basis for dissecting the regulatory mechanisms of other indolocarbazole compounds with clinical application value.

关键词： transcriptional regulation lead compound antibiotics antitumor gene cluster

Microglial glutaminase 1 mediates chronic restraint stress-induced depression-like behaviors and synaptic damages

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Signal Transduction and Targeted Therapy 2024年第1期9卷 40-43页

作者： Huili Chen Shengyang Fu Xiangyu Li Meng Shi jiazhen Qian Shu Zhao Ping Yuan Lu Ding Xiaohuan Xia Jialin C.zheng Center for Translational Neurodegeneration and Regenerative Therapy Tongji Hospital affiliated to Tongji University School of Medicine200065 ShanghaiChina Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Translational Research Institute of Brain and Brain-Like Intelligence Shanghai Fourth People’s Hospital affiliated to Tongji University School of Medicine200434 ShanghaiChina

Dear Editor,Major depressive disorder(MDD)is one of the most common psychiatric illnesses that significantly increase the risk of suicide.1 Stress-triggered dysfunctions of microglia have been identified as a commonly occurred pathological feature of MDD.1–3 Microglial dysfunction contributes to the pathogenesis of MDD via immunoresponses/neuroinflammation-mediated neural damage and pathological synapse loss-mediated neural circuit disruption.1 Although the involvement of microglia in MDD has been widely investigated,the molecular mechanisms underlying microglial dysfunction remain largely ***,we identified glutaminase 1(Gls1)as one key protein that participates in microglial dysfunction.4–6 Gls1 catalyzes the hydrolysis of glutamine to produce glutamate in the brain.4 Besides its well-known role in excitatory neurotoxicity,we found Gls1 up-regulation in microglia in animal models of Alzheimer’s disease and ischemic stroke.4,7 Gls1 activates microglia to overproduce cytokines and release inflammatory extracellular vesicles,therefore leading to neuroinflammation in animal models of Alzheimer’s disease and ischemic stroke.4–6 More importantly,Gls1 has been found to be up-regulated in the brains of MDD patients,and microglial Gls1 deficiency mitigated LPS-induced depression-like behaviors.8 However,LPS exposure is not an appropriate model to mimic MDD phenotypes,leaving the involvement of Gls1 in MDD an undetermined question.

关键词： involvement inflammation media

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纳米级过硫酸铵微胶囊的制备及性能

石油学报（石油加工） 2020年第4期36卷 720-727页

作者：李晓丹李光辉郑家桢周伟康燕山大学车辆与能源学院河北秦皇岛066004

通过原位聚合法成功制备了以过硫酸铵为芯材、聚吡咯为壳材的微胶囊,并通过扫描电镜、激光粒度仪、红外光谱和拉曼光谱分析微胶囊的形貌和结构。结果表明:微胶囊的粒径分布集中在100~300 nm,且聚吡咯成功包覆过硫酸铵。微胶囊在水中分... 详细信息

通过原位聚合法成功制备了以过硫酸铵为芯材、聚吡咯为壳材的微胶囊,并通过扫描电镜、激光粒度仪、红外光谱和拉曼光谱分析微胶囊的形貌和结构。结果表明:微胶囊的粒径分布集中在100~300 nm,且聚吡咯成功包覆过硫酸铵。微胶囊在水中分散时的电导率的变化过程说明微胶囊的释放过程为渗透释放;且随着甘油量的增加,微胶囊吸水速率加快,内部固体过硫酸铵溶解速率提高,对应过硫酸铵的释放速率提高。将过硫酸铵与等质量的微胶囊分别加入聚合物压裂液中,通过高温降黏实验,发现该微胶囊能延迟破胶7 h以上。

关键词：微胶囊核壳型破胶剂原位聚合法

改性纳米硅颗粒强化高温泡沫的性能及机理研究

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材料导报 2023年第7期37卷 243-247页

作者：郑家桢裴海华张贵才单景玲蒋平中国石油大学(华东)石油工程学院山东青岛266580 中国石油大学(华东)非常规油气开发教育部重点实验室山东青岛266580

高温环境下常规两相泡沫的泡沫稳定性很差,不能满足高温油藏生产需求。本工作以改性纳米硅颗粒(NP)强化α-烯烃磺酸钠(AOS)的泡沫性能,通过Waring Blender法对NP强化泡沫体系的泡沫性能进行评价,研究了NP稳定AOS泡沫的机理和NP强化泡沫... 详细信息

高温环境下常规两相泡沫的泡沫稳定性很差,不能满足高温油藏生产需求。本工作以改性纳米硅颗粒(NP)强化α-烯烃磺酸钠(AOS)的泡沫性能,通过Waring Blender法对NP强化泡沫体系的泡沫性能进行评价,研究了NP稳定AOS泡沫的机理和NP强化泡沫高温下的封堵能力。结果表明,2.0%NP强化的0.5%AOS泡沫具有十分稳定的泡沫性能,高温热老化处理后泡沫性能稳定;其稳泡机理在于NP能在气液界面上形成不可逆吸附,同时NP能与AOS分子发生吸附,有效提高起泡溶液的黏度、表面扩张弹性模量和储能模量,增强液膜强度,延缓泡沫的排液、歧化和聚并;NP强化泡沫在1000 mD和3000 mD渗透率时均具有优秀的封堵性能,其阻力因子远大于AOS泡沫,具有高温调驱应用潜力。

关键词：多相泡沫纳米硅颗粒流变性表面扩张黏弹性调驱剂

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稠油蒸汽驱封窜剂/驱油剂组合调驱技术

油田化学 2022年第1期39卷 87-92页

作者：单景玲裴海华郑伟谢昊君郑家桢张贵才中国石油大学(华东)石油工程学院山东青岛266580 非常规油气开发教育部重点实验室(中国石油大学(华东)) 山东青岛266580 中海油研究总院有限责任公司北京100028

针对稠油蒸汽驱汽窜严重问题,研究了高温封窜剂+驱油剂组合调驱技术,以高温栲胶体系为蒸汽封窜剂,以磺酸盐表面活性剂SLB和阴-非离子表面活性剂CY复配体系为高温驱油剂,采用非均质双管物理模拟实验评价了封窜剂+驱油剂组合调驱提高蒸汽... 详细信息

针对稠油蒸汽驱汽窜严重问题,研究了高温封窜剂+驱油剂组合调驱技术,以高温栲胶体系为蒸汽封窜剂,以磺酸盐表面活性剂SLB和阴-非离子表面活性剂CY复配体系为高温驱油剂,采用非均质双管物理模拟实验评价了封窜剂+驱油剂组合调驱提高蒸汽驱采收率效果。结果表明,高温栲胶封窜剂在250℃蒸汽驱冲刷15 PV后封堵率仍可达90%以上,封堵效果显著;SLB/CY(复配比2∶1)高温驱油剂经过300℃老化后仍可使油水界面张力降到10^(-3)mN/m以下。采用封窜剂+驱油剂组合调驱体系可有效封堵高渗管汽窜通道,保证后续注入驱油剂进入低渗管,最终综合采收率可以提高23.58%,有效地改善非均质地层蒸汽驱的开发效果。

关键词：稠油蒸汽驱封窜剂高温驱油剂提高采收率

混合溶剂中混合电解质活度系数的研究——HCl+NaCl+PrOH+H2O体系,298.15K

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物理化学学报 1985年第5期1卷 476-481页

作者：初明晨杨家振梁春余郑溪娟诸若瑶辽宁大学化学系辽宁大学化学系沈阳沈阳大学

电动势法是测定混合电解质活度系数时常用的主要方法,曾用这种方法对混合电解质水溶液活度系数作过火量的研究。可是,在有机溶剂和水的混合溶剂中混合电解质的电动势数据和活度系数数据却很少。Akerlof等人在这方面进行了开创性的研究工... 详细信息

电动势法是测定混合电解质活度系数时常用的主要方法,曾用这种方法对混合电解质水溶液活度系数作过火量的研究。可是,在有机溶剂和水的混合溶剂中混合电解质的电动势数据和活度系数数据却很少。Akerlof等人在这方面进行了开创性的研究工作,他们在恒定总

关键词：活度系数电动势热力学性质混合电解质 HCl+NaCl+PrOH+H2O 混合溶剂

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稠油蒸汽驱封窜剂/洗油剂复合调驱技术

油田化学 2020年第4期37卷 697-700页

作者：舒展裴海华张贵才曹旭郑家祯蒋平中国石油大学(华东)石油工程学院山东青岛266580

针对稠油蒸汽驱波及系数低、洗油效率低的问题,用石油树脂颗粒、阴离子型表面活性剂和水溶性高分子聚合物制备了耐高温封窜剂,将羧酸盐表面活性剂和磺酸盐表面活性剂按质量比1.5:1复配制得洗油剂。通过物理模拟实验研究了封窜剂和洗油... 详细信息

针对稠油蒸汽驱波及系数低、洗油效率低的问题,用石油树脂颗粒、阴离子型表面活性剂和水溶性高分子聚合物制备了耐高温封窜剂,将羧酸盐表面活性剂和磺酸盐表面活性剂按质量比1.5:1复配制得洗油剂。通过物理模拟实验研究了封窜剂和洗油剂复合调驱技术,考察了封窜剂的注入性和封堵性,以及复合调驱体系提高蒸汽驱采收率的能力。结果表明,封窜剂注入性和封堵性较好,在250℃高温下具有较好的耐冲刷性能。对于渗透率大于3.0μm^2的地层,封窜剂具有较好的注入性能;对于渗透率约为5.0μm^2的岩心,封堵率均能保持在90%以上,并且在250℃蒸汽冲刷20 PV后,封堵率降低值低于2%。洗油剂可使油水界面张力达到10^(-3) mN/m数量级,在90~250℃的洗油效率大于40%。蒸汽驱中单独使用洗油剂仅能提高采收率14%,而采用封窜剂和洗油剂复合调驱体系可提高采收率26%。稠油蒸汽驱中采用封窜剂和洗油剂复合调驱技术可有效封堵汽窜通道,提高驱油效率,改善稠油油藏蒸汽驱的开发效果。图2表4参15。

关键词：稠油蒸汽驱汽窜封窜剂洗油剂采收率

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三相泡沫调驱体系提高蒸汽驱采收率

油田化学 2022年第1期39卷 82-86,92页

作者：郑家桢张贵才裴海华单景玲蒋平吴晗中国石油大学(华东)石油工程学院山东青岛266580 非常规油气开发教育部重点实验室(中国石油大学(华东)) 山东青岛266580

为解决蒸汽驱过程中发生的蒸汽超覆和汽窜问题,以磺酸盐表面活性剂ZAS、聚醚磺酸盐表面活性剂ZCP-1和改性纳米硅颗粒NS复配研制了配方为0.5%ZAS/ZCP-1(复配比3∶1)+1.0%NS的三相泡沫调驱体系,并通过实验评价该体系的泡沫性能、耐温性能... 详细信息

为解决蒸汽驱过程中发生的蒸汽超覆和汽窜问题,以磺酸盐表面活性剂ZAS、聚醚磺酸盐表面活性剂ZCP-1和改性纳米硅颗粒NS复配研制了配方为0.5%ZAS/ZCP-1(复配比3∶1)+1.0%NS的三相泡沫调驱体系,并通过实验评价该体系的泡沫性能、耐温性能、封堵性能和驱油性能。研究结果表明,该三相泡沫体系的泡沫性能优异,常温下泡沫体积为680 mL、析液半衰期为26.67 min、泡沫半衰期为12 h,300℃热老化处理后性能稳定;该三相泡沫体系在300℃高温条件下可以稳定存在且具有较好的封堵性能,能选择性封堵高渗透层,对不同渗透率(1000×10^(-3)~4000×10^(-3)μm^(2))填砂管的阻力因子均大于30,阻力因子随着岩心渗透率的增加而增大。在渗透率级差为1∶2的非均质条件下可有效改善吸汽剖面,大幅提高低渗模型采收率,综合采收率可提高17.93%,具有良好的提高蒸汽驱采收率潜力。

关键词：三相泡沫蒸汽驱调驱剂改性纳米硅颗粒提高采收率