Multi-omics Data Reveal the Effect of Sodium Butyrate on Gene Expression and Protein Modification in Streptomyces

作     者：Jiazhen Zheng Yue Li Ning Liu Jihui Zhang Shuangjiang Liu Huarong Tan Jiazhen Zheng;Yue Li;Ning Liu;Jihui Zhang;Shuangjiang Liu;Huarong Tan

作者机构：State Key Laboratory of Microbial ResourcesInstitute of MicrobiologyChinese Academy of SciencesBeijing 100101China College of Life SciencesUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Microbial BiotechnologyShandong UniversityQingdao 266237China 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2023年第21卷第6期

页      面：1149-1162页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0711[理学-系统科学] 07[理学] 08[工学] 

基　　金：supported by the National Key R&D Program of China(Grant No.2020YFA0907800) the National Natural Science Foundation of China(Grant Nos.32170043 and 82173720) 

主　　题：Silent gene cluster Streptomyces Sodium butyrate Protein modification Acetylome 

摘      要：Streptomycetes possess numerous gene clusters and the potential to produce a large amount of natural *** deacetylase(HDAC)inhibitors play an important role in the regulation of histone modifications in fungi,but their roles in prokaryotes remain poorly ***,we investigated the global effects of the HDAC inhibitor,sodium butyrate(SB),on marine-derived Streptomyces olivaceus FXJ 8.021,particularly focusing on the activation of secondary metabolite *** antiSMASH analysis revealed 33 secondary metabolite biosynthetic gene clusters(BGCs)in strain FXJ 8.021,among which the silent lobophorin BGC was activated by *** data showed that the expression of genes involved in lobophorin biosynthesis(ge00097–ge00139)and CoA-ester formation(e.g.,ge02824),as well as the glycolysis/gluconeogenesis pathway(e.g.,ge01661),was significantly up-regulated in the presence of *** CoA-ester analysis confirmed that SB triggered the biosynthesis of CoA-ester,thereby increasing the precursor supply for lobophorin *** acetylomic analysis revealed that the acetylation levels on 218 sites of 190 proteins were up-regulated and those on 411 sites of 310 proteins were *** acetylated proteins were particularly enriched in transcriptional and translational machinery components(e.g.,elongation factor GE04399),and their correlations with the proteins involved in lobophorin biosynthesis were established by protein–protein interaction network analysis,suggesting that SB might function via a complex hierarchical.