检索结果-南通市图书馆

Alpine grassland degradation intensifies the burrowing behavior of small mammals:evidence for a negative feedback loop

引用

Integrative Zoology 2024年第2期19卷 240-252页

作者： Zaiwei WANG jiawen yan Amy MARTIN Dianne HBRUNTON Jiapeng QU Jin-Sheng HE Weihong JI Zhibiao NAN State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouChina Manaaki Whenua-Landcare Research LincolnNew Zealand School of Natural Sciences Massey University(Albany Campus)AucklandNew Zealand Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina Qinghai Haibei National Field Research Station of Alpine Grassland Ecosystem Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina Institute of Ecology College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking UniversityBeijingChina

Globally,grassland degradation is an acute ecological problem.In alpine grassland on the Tibetan Plateau,increased densities of various small mammals in degraded grassland are assumed to intensify the degradation process and these mammals are subject to lethal control.However,whether the negative impact of small mammals is solely a result of population size or also a result of activity and behavior has not been tested.In this study,we use plateau pika as a model to compare population size,core area of colony,and the number of burrow entrances and latrines between lightly and severely degraded grassland.We test whether the alleged contribution of pika to grassland degradation is a result of increased population size or increased burrowing activities of individuals in response to lower food abundance.We found that grassland degradation resulted in lower plant species richness,plant height,and biomass.Furthermore,the overall population size of pika was not significantly affected by location in lightly and severely degraded grassland.However,pika core areas in severely grassland degradation were significantly larger and had significantly higher densities of burrows and latrines.Our study provides convincing evidence that habitat-induced changes in the behavior of small,burrowing mammals,such as pika,can exacerbate grassland degradation.This finding has significant implications for managing small mammals and restoring degraded grassland ecosystems.

关键词： burrowing behavior grassland degradation plateau pika population size small mammals

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ultrasound-guided prostate percutaneous intervention robot system and calibration by informative particle swarm optimization

引用

Frontiers of Mechanical Engineering 2022年第1期17卷 18-33页

作者： jiawen yan Bo PAN Yili FU State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 150001China

Applying a robot system in ultrasound-guided percutaneous intervention is an effective approach for prostate cancer diagnosis and treatment.The limited space for robot manipulation restricts structure volume and motion.In this paper,an 8-degree-of-freedom robot system is proposed for ultrasound probe manipulation,needle positioning,and needle insertion.A novel parallel structure is employed in the robot system for space saving,structural rigidity,and collision avoidance.The particle swarm optimization method based on informative value is proposed for kinematic parameter identification to calibrate the parallel structure accurately.The method identifies parameters in the modified kinematic model stepwise according to parameter discernibility.Verification experiments prove that the robot system can realize motions needed in targeting.By applying the calibration method,a reasonable,reliable forward kinematic model is built,and the average errors can be limited to 0.963 and 1.846 mm for insertion point and target point,respectively.

关键词： ultrasound image guidance prostate percutaneous intervention parallel robot kinematics identification particle swarm optimization informative value

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The salt-activated CBF1/CBF2/CBF3-GALS1 module fine-tunes galactan-induced salt hypersensitivity in Arabidopsis

引用

Journal of Integrative Plant Biology 2023年第8期65卷 1904-1917页

作者： Jingwei yan Ya Liu jiawen yan Zhihui Liu Heqiang Lou Jiasheng Wu State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China

Plant growth and development are significantly hampered in saline environments,limiting agricultural productivity.Thus,it is crucial to unravel the mechanism underlying plant responses to salt stress.β-1,4-Galactan(galactan),which forms the side chains of pectic rhamnogalacturonan I,enhances plant sensitivity to high-salt stress.Galactan is synthesized by GALACTAN SYNTHASE1(GALS1).We previously showed that Na Cl relieves the direct suppression of GALS1 transcription by the transcription factors BPC1 and BPC2 to induce the excess accumulation of galactan in Arabidopsis(Arabidopsis thaliana).However,how plants adapt to this unfavorable environment remains unclear.Here,we determined that the transcription factors CBF1,CBF2,and CBF3 directly interact with the GALS1 promoter and repress its expression,leading to reduced galactan accumulation and enhanced salt tolerance.Salt stress enhances the binding of CBF1/CBF2/CBF3 to the GALS1 promoter by inducing CBF1/CBF2/CBF3 transcription and accumulation.Genetic analysis suggested that CBF1/CBF2/CBF3 function upstream of GALS1 to modulate salt-induced galactan biosynthesis and the salt response.CBF1/CBF2/CBF3 and BPC1/BPC2 function in parallel to regulate GALS1 expression,thereby modulating the salt response.Our results reveal a mechanism in which salt-activated CBF1/CBF2/CBF3 inhibit BPC1/BPC2-regulated GALS1 expression to alleviate galactan-induced salt hypersensitivity,providing an activation/deactivation fine-tune mechanism for dynamic regulation of GALS1 expression under salt stress in Arabidopsis.

关键词： Arabidopsis thaliana BPC1/BPC2 CBF1/CBF2/CBF3 β-1,4-galactan GALS1 salt stress

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Arabidopsis mitochondrial single-stranded DNA-binding proteins SSB1 and SSB2 are essential regulators of mtDNA replication and homologous recombination

引用

Journal of Integrative Plant Biology 2022年第10期64卷 1952-1965页

作者： Jie Qian Min Zheng Lingyu Wang Yu Song jiawen yan Yi‐Feng Hsu School of Life Sciences Southwest UniversityChongqing 400715China

Faithful DNA replication is one of the most essential processes in almost all living organisms.However,the proteins responsible for organellar DNA replication are still largely unknown in plants.Here,we show that the two mitochondriontargeted single-stranded DNA-binding(SSB)proteins SSB1 and SSB2 directly interact with each other and act as key factors for mitochondrial DNA(mt DNA)maintenance,as their single or double loss-of-function mutants exhibit severe germination delay and growth retardation.The mt DNA levels in mutants lacking SSB1 and/or SSB2 function were two-to four-fold higher than in the wild-type(WT),revealing a negative role for SSB1/2 in regulating mt DNA replication.Genetic analysis indicated that SSB1 functions upstream of mitochondrial DNA POLYMERASE IA(POLIA)or POLIB in mt DNA replication,as mutation in either gene restored the high mt DNA copy number of the ssb1-1 mutant back to WT levels.In addition,SSB1 and SSB2 also participate in mitochondrial genome maintenance by influencing mt DNA homologous recombination(HR).Additional genetic analysis suggested that SSB1 functions upstream of ORGANELLAR SINGLE-STRANDED DNABINDING PROTEIN1(OSB1)during mt DNA replication,while SSB1 may act downstream of OSB1and MUTS HOMOLOG1 for mt DNA HR.Overall,our results yield new insights into the roles of the plant mitochondrion-targeted SSB proteins and OSB1 in maintaining mt DNA stability via affecting DNA replication and DNA HR.

关键词： Arabidopsis mitochondria mtDNA replication mtDNA recombination SSB

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

General closed-form inverse kinematics for arbitrary three-joint subproblems based on the product of exponential model

引用

Frontiers of Mechanical Engineering 2022年第2期17卷 85-101页

作者： Tao SONG Bo PAN Guojun NIU jiawen yan Yili FU State Key Laboratory of Robotics and System Harbin Institute of TechnologyHarbin 150001China School of Mechanical Engineering and Automation Zhejiang Sci-Tech UniversityHangzhou 310018China

The inverse kinematics problems of robots are usually decomposed into several Paden–Kahan subproblems based on the product of exponential model. However, the simple combination of subproblems cannot solve all the inverse kinematics problems, and there is no common approach to solve arbitrary three-joint subproblems in an arbitrary postural relationship. The novel algebraic geometric (NAG) methods that obtain the general closed-form inverse kinematics for all types of three-joint subproblems are presented in this paper. The geometric and algebraic constraints are used as the conditions precedent to solve the inverse kinematics of three-joint subproblems. The NAG methods can be applied in the inverse kinematics of three-joint subproblems in an arbitrary postural relationship. The inverse kinematics simulations of all three-joint subproblems are implemented, and simulation results indicating that the inverse solutions are consistent with the given joint angles validate the general closed-form inverse kinematics. Huaque III minimally invasive surgical robot is used as the experimental platform for the simulation, and a master–slave tracking experiment is conducted to verify the NAG methods. The simulation result shows the inverse solutions and six sets given joint angles are consistent. Additionally, the mean and maximum of the master–slave tracking experiment for the closed-form solution are 0.1486 and 0.4777 mm, respectively, while the mean and maximum of the master–slave tracking experiment for the compensation method are 0.3188 and 0.6394 mm, respectively. The experiments results demonstrate that the closed-form solution is superior to the compensation method. The results verify the proposed general closed-form inverse kinematics based on the NAG methods.

关键词： inverse kinematics Paden-Kahan subproblems three-joint subproblems product of exponential closed-form solution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

论醉酒型危险驾驶罪定罪情节的发展与完善

引用

北京政法职业学院学报 2024年第2期 26-31页

作者：刘娜颜佳文青岛科技大学法学院山东省青岛市市北区人民检察院政治部

醉驾在我国入刑后,其定罪的标准经历了从单一定罪情节到多元定罪情节的转变,主要包括血液中酒精含量、隔夜醉酒驾驶、醉酒后短距离挪车等方面。但是,对于该罪的定罪情节,尚未形成全国一致的明确标准,不利于该罪的司法实践统一。本文提出... 详细信息

醉驾在我国入刑后,其定罪的标准经历了从单一定罪情节到多元定罪情节的转变,主要包括血液中酒精含量、隔夜醉酒驾驶、醉酒后短距离挪车等方面。但是,对于该罪的定罪情节,尚未形成全国一致的明确标准,不利于该罪的司法实践统一。本文提出,可以在遵循抽象危险犯判断的基本原则的前提下,统一该罪酒精含量、隔夜醉酒驾驶、醉酒挪车三项主要定罪情节的认定标准,以期统一该罪定罪情节的认定标准,发挥更好的社会治理效果。

关键词：醉酒型危险驾驶罪抽象危险犯酒精含量隔夜驾驶醉酒挪车

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prognostic value of the long noncoding RNA AFAP1-AS1 in cancers

引用

Oncology and Translational Medicine 2023年第3期9卷 133-146页

作者： Lixiu Zhu jiawen yan Guoqiang Xu Qiaoli Wang Tianrui Xu Ruixue Cao Chuanzheng Sun yan Xi Wei Xiong Department of Radiotherapy Yunnan Cancer HospitalThe Third Affiliated Hospital of Kunming Medical UniversityKunming 650000China Department of Radiotherapy People's Hospital of Yuxi CityThe Sixth Affiliated Hospital of Kunming Medical UniversityYuxi 653100China Department of Head and Neck Surgery Yunnan Cancer HospitalThe Third Affiliated Hospital of Kunming Medical UniversityKunming 650000China

Objective This meta-analysis explored whether the expression of actin filament-associated protein 1 antisense RNA 1(AFAP1-AS1)is related to the prognosis and clinicopathological features of patients with cancer.Methods PubMed,EMBASE,and Cochrane Library were systematically searched.Hazard ratios(HRs)with 95%confidence intervals(CIs)were used to assess the prognostic value based on overall survival(OS),disease-free survival(DFS),and progression-free survival(PFS).Odds ratios(ORs)with 95%CIs were used to determine the relationships between AFAP1-AS1 and clinicopathological features,such as large tumor size(LTS),high tumor stage(HTS),poor histological grade(PHG),lymph node metastasis(LNM),and distant metastasis(DM).Results Thirty-five eligible articles and 3433 cases were analyzed.High AFAP1-AS1 expression,compared to low AFAP1-AS1 expression,correlated with significantly shorter OS(HR=2.15,95%CI=1.97-2.34,P<0.001),DFS(HR=1.37,95%CI=1.19-1.57,P<0.001),and PFS(HR=1.97,95%CI=1.56-2.50,P<0.001)in patients with cancer.In various cancers,elevated AFAP1-AS1 expression was significantly associated with LTS(OR=2.76,95%CI=2.16-3.53,P<0.001),HTS(OR=2.23,95%CI=1.83-2.71,P<0.001),and PHG(OR=1.39,95%CI=1.08-1.79,P=0.01)but not LNM(OR=1.59,95%CI=0.88-2.85,P=0.12)or DM(OR=1.81,95%CI=0.90-3.66,P=0.10).Conclusion High AFAP1-AS1 expression was associated with prognostic and clinicopathological features,suggesting that AFAP1-AS1 is a prognostic biomarker for human cancers.

关键词： long noncoding RNA(lncRNA) actin filament-associated protein 1 antisense RNA 1(AFAP1-AS1) prognostic meta-analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

华中樱磷酸甲羟戊酸激酶基因PcoPMK克隆与表达分析

引用

中南林业科技大学学报 2024年第3期44卷 117-125页

作者：舒东膂李建挥禹霖柏文富杨扬胡景堃严佳文湖南省植物园湖南长沙410116

【目的】克隆华中樱磷酸甲羟戊酸激酶基因PcoPMK的cDNA序列,分析该基因在华中樱‘YS01’不同发育阶段花瓣中的表达情况及其编码蛋白质的结构特征和理化性质,为进一步研究PcoPMK基因在华中樱萜烯类物质合成途径中的功能提供参考。【方法... 详细信息

【目的】克隆华中樱磷酸甲羟戊酸激酶基因PcoPMK的cDNA序列,分析该基因在华中樱‘YS01’不同发育阶段花瓣中的表达情况及其编码蛋白质的结构特征和理化性质,为进一步研究PcoPMK基因在华中樱萜烯类物质合成途径中的功能提供参考。【方法】参照近缘物种的PMK同源基因cDNA保守序列设计特异性引物,应用RT-PCR技术扩增PcoPMK基因的cDNA序列,并进行克隆测序,同时应用定量RT-PCR技术分析该基因在‘YS01’不同发育阶段花瓣中的表达情况。应用ORF Finder在线工具预测基因的开放阅读框及编码的蛋白质序列;分别应用ExPASy、SOPMA、Swiss model和CDD在线工具分析蛋白质的理化性质、二级、三级结构和功能结构域;分别应用DeepTMHMM、SINALP 4.0 Server和PredictProtein在线程序预测蛋白质的跨膜结构、信号肽和亚细胞定位;应用MEGA7.0软件构建基因的系统进化树。【结果】华中樱PcoPMK基因开放阅读框序列大小为1530 bp,编码509个氨基酸,GenBank登录号为OR373074。编码蛋白质的相对分子量为55.199 kD,理论等电点为5.68,属于亲水性的稳定蛋白,定位于细胞质。PcoPMK蛋白与扁桃、烟草和拟南芥等代表性物种PMK同源蛋白的序列相似度较高,含有3个典型且保守的蛋白结构功能域和1个ATP结合位点,无跨膜螺旋和信号肽,属于非分泌蛋白。PcoPMK基因在华中樱‘YS01’盛开期花瓣中的相对表达量显著高于花蕾期和半开期(P<0.05),与同时期花瓣中萜烯类香气物质的含量变化规律一致。【结论】本研究成功克隆了华中樱PcoPMK基因,初步推测其可能在花瓣萜烯类物质合成途径中发挥重要作用,为进一步研究该基因的生物学功能提供了参考。

关键词：华中樱磷酸甲羟戊酸激酶基因 RT-PCR 基因克隆

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

一种智能变电站风险评估模型研究与分析

引用

微型电脑应用 2024年第1期40卷 84-87页

作者：闫佳文周磊蒋春悦陈长金刘哲国网河北省电力有限公司培训中心河北石家庄050031 北京科东电力控制系统有限责任公司北京100192

针对当前智能变电站具备信息物理系统的特点,考虑到传统变电站风险评估缺乏信息系统风险分析,提出一种CPS下的变电站风险评估模型。通过分析变电站可能存在的故障因素,设计基于CPS的变电站风险转移概率模型。根据概率转移模型建立变电... 详细信息

针对当前智能变电站具备信息物理系统的特点,考虑到传统变电站风险评估缺乏信息系统风险分析,提出一种CPS下的变电站风险评估模型。通过分析变电站可能存在的故障因素,设计基于CPS的变电站风险转移概率模型。根据概率转移模型建立变电站风险评估模型,并用改进的缎蓝园丁鸟优化算法(SBO)算法求解评估模型,从而量化变电站风险指数。在实验中,以典型IEEE智能CPS变电站进行仿真,仿真结果验证了所提模型的有效性及实用性。

关键词：智能变电站信息物理系统风险评估缎蓝园丁鸟优化算法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Rapid Identification Method for Germplasm Materials Resistant to Citrus Huanglongbing Disease

引用

Agricultural Biotechnology 2020年第6期9卷 64-69页

作者： yan TANG Xiaoxiao WU Binghai LOU Ping LIU Chuanwu CHEN Ying NIU Gebi ZHANG jiawen yan Chongling DENG Guangxi Academy of Specialty Crops/Guangxi Citrus Breeding and Cultivation Research Center of Engineering Technology Guilin 541004China College of Life Science Guangxi Normal UniversityGuilin 541004China

[Objectives]This study was conducted to develop a rapid identification method for citrus germline materials resistant to Huanglongbing disease and lay a basis for accelerating citrus breeding for resistance to Huanglongbing and increasing the breeding efficiency.[Methods]Thirty-six citrus germplasms suspected to be resistant to citrus Huanglongbing disease were collected.The method of direct high grafting to citrus trees infected with Huanglongbing pathogen was adopted.The resistance of the test materials was identified and evaluated by field symptoms combined with quantitative PCR.It was defined as the top grafting identification method.[Results]The test materials that were grafted in spring started to germinate after one month,and three months late(June 5,2018)typical mottled yellowing on leaves was observed on KH-14 for the first time.After four months(July 5,2018)of top grafting,typical mottled yellowing occurred on 23 materials,and 11 materials showed no such symptom.After six months(September 4,2018)of top grafting,although the growth of KH-18,KH-12,KHY-4,KHY-5 and KHY-6 were normal,yellowing was observed on their leaves.Only KH-21 grew well,and showed no yellow shoots and yellowing leaves.It was identified as the material with resistance to Huanglongbing disease.Quantitative PCR tests on the above six materials showed that KH-21 was negative and other five were positive.Real-time fluorescence quantitative PCR test indicated that the average Huanglongbing bacteria amount in KH-21 was 1870.0 cell/μg DNA,and the average Huanglongbing bacteria amount in the control material was 372285.5 cell/μg DNA,indicating KH-21 was resistant to Huanglongbing bacteria.[Conclusions]The method for infecting bacteria by top grafting takes six months,can detect large amount of seedlings,and is time-saving,efficient,cost-saving and accurate.This method can quickly identify the resistance of citrus varieties to citrus Huanglongbing disease,and can be popularized and used in the identification of citrus Huanglongbing disease resis

关键词： Citrus Huanglongbing disease Resistance Germplasm material Method for infecting trees with bacteria by top grafting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：