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Integrated Au-Nanoroded Biosensing and Regulating Platform for Photothermal Therapy of Bradyarrhythmia

Research 2022年第3期2022卷 31-44页

作者： jiaru fang Dong Liu Dongxin Xu Qianni Wu Hongbo Li Ying Li Ning Hu State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and TechnologySchool of Electronics and Information TechnologyState Key Laboratory of OphthalmologyZhongshan Ophthalmic CenterGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceSun Yatsen UniversityGuangzhou 510006China Stoddart Institute of Molecular Science Department of ChemistryZJU-Hangzhou Global Scientific and Technological Innovation CenterZhejiang UniversityHangzhou 310058China Molecular Cancer Research Center School of MedicineSun Yat-sen UniversityShenzhen 518107China State Key Laboratory of Transducer Technology Chinese Academy of SciencesShanghai 200050China

Bradyarrhythmia is a kind of cardiovascular disease caused by dysregulation of cardiomyocytes,which seriously threatens human ***,treatment strategies of bradyarrhythmia mainly include drug therapy,surgery,or implantable cardioverter defibrillators,but these strategies are limited by drug side effect,surgical trauma,and instability of implanted ***,we developed an integrated Au-nanoroded biosensing and regulating platform to investigate the photothermal therapy of cardiac bradyarrhythmia in ***-nanoroded electrode array can simultaneously accumulate energy from the photothermal regulation and monitor the electrophsiological state to restore normal rhythm of cardiomyocytes in real *** treat the cardiomyocytes cultured on Au-nanoroded device by near-infrared(NIR)laser irradiation,cardiomyocytes return to normal for long term after irradiation of suitable NIR energy and *** with the conventional strategies,the photothermal strategy is more effective and convenient to regulate the ***,mRNA sequencing shows that the differential expression genes in cardiomyocytes are significantly increased after photothermal strategy,which are involved in the regulation of the heart rate,cardiac conduction,and ion *** work establishes a promising integrated biosensing and regulating platform for photothermal therapy of bradyarrhythmia in vitro and provides reliable evidence of photothermal regulation on cardiomyocytes for cardiological clinical studies.

关键词： arrhythmia return conduction

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Semi-Implantable Bioelectronics

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Nano-Micro Letters 2022年第7期14卷 354-408页

作者： jiaru fang Shuang Huang Fanmao Liu Gen He Xiangling Li Xinshuo Huang Hui-jiuan Chen Xi Xie State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and TechnologySchool of Electronics and Information TechnologySun Yat-Sen UniversityGuangzhou 510006People’s Republic of China

Developing techniques to effectively and real-time monitor and regulate the interior environment of biological objects is significantly important for many biomedical engineering and scientific applications, including drug delivery, electrophysiological recording and regulation of intracellular activities. Semi-implantable bioelectronics is currently a hot spot in biomedical engineering research area, because it not only meets the increasing technical demands for precise detection or regulation of biological activities, but also provides a desirable platform for externally incorporating complex functionalities and electronic integration. Although there is less definition and summary to distinguish it from the well-reviewed non-invasive bioelectronics and fully implantable bioelectronics, semi-implantable bioelectronics have emerged as highly unique technology to boost the development of biochips and smart wearable device. Here, we reviewed the recent progress in this field and raised the concept of “Semi-implantable bioelectronics”, summarizing the principle and strategies of semi-implantable device for cell applications and in vivo applications, discussing the typical methodologies to access to intracellular environment or in vivo environment, biosafety aspects and typical applications. This review is meaningful for understanding in-depth the design principles, materials fabrication techniques, device integration processes, cell/tissue penetration methodologies, biosafety aspects, and applications strategies that are essential to the development of future minimally invasive bioelectronics.

关键词： Semi-implantable bioelectronics Cell applications In vivo applications

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Cardiomyocyte electrical-mechanical synchronized model for high-content, dose-quantitative and time-dependent drug assessment

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Microsystems & Nanoengineering 2021年第2期7卷 131-142页

作者： jiaru fang Xinwei Wei Hongbo Li Ning Hu Xingxing Liu Dongxin Xu Tao Zhang Hao Wan Ping Wang Xi Xie The First Affiliated Hospital of Sun Yat-Sen University School of Electronics and Information TechnologyGuangdong Province Key Laboratory of Display Material and TechnologySun Yat-sen UniversityGuangzhou510006China Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of EducationDepartment of Biomedical EngineeringZhejiang UniversityHangzhou310027China State Key Laboratory of Transducer Technology Chinese Academy of SciencesShanghai200050China School of Biomedical Engineering Sun Yat-sen UniversityGuangzhou510006China

Cardiovascular diseases have emerged as a significant threat to human ***,drug development is a time-consuming and costly process,and few drugs pass the preclinical assessment of safety and *** existing patch-clamp,Ca2+imaging,and microelectrode array technologies in cardiomyocyte models for drug preclinical screening have suffered from issues of low throughput,limited long-term assessment,or inability to synchronously and correlatively analyze electrical and mechanical ***,we develop a high-content,dose-quantitative and time-dependent drug assessment platform based on an electrical-mechanical synchronized(EMS)biosensing *** microfabricated EMS can record both firing potential(FP)and mechanical beating(MB)signals from cardiomyocytes and extract a variety of characteristic parameters from these two signals(FP–MB)for further *** system was applied to test typical ion channel drugs(lidocaine and isradipine),and the dynamic responses of cardiomyocytes to the tested drugs were recorded and *** high-throughput characteristics of the system can facilitate simultaneous experiments on a large number of ***,a database of various cardiac drugs can be established by heat map analysis for rapid and effective screening of *** EMS biosensing system is highly promising as a powerful tool for the preclinical development of new medicines.

关键词： electrical consuming patch

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Recognition of high-specificity hERG K+ channel inhibitor-induced arrhythmia in cardiomyocytes by automated template matching

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Microsystems & Nanoengineering 2021年第2期7卷 107-119页

作者： Hao Wang Hongbo Li Xinwei Wei Tao Zhang Yuting Xiang jiaru fang Peiran Wu Xi Xie Ping Wang Ning Hu The First Affiliated Hospital of Sun Yat-sen University School of Electronics and Information TechnologyState Key Laboratory of Optoelectronic Materials and TechnologiesGuangdong Province Key Laboratory of Display Material and TechnologySun Yat-sen UniversityGuangzhou510006China Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of EducationDepartment of Biomedical EngineeringZhejiang UniversityHangzhou310027China The Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou510655China State Key Laboratory of Transducer Technology Chinese Academy of SciencesShanghai200050China

Cardiovascular disease(CVD)is the number one cause of death in *** induced by gene mutations,heart disease,or hERG K+channel inhibitors is a serious CVD that can lead to sudden death or heart *** cardiomyocyte-based biosensors can record extracellular potentials and mechanical beating ***,parameter extraction and examination by the naked eye are the traditional methods for analyzing arrhythmic beats,and it is difficult to achieve automated and efficient arrhythmic recognition with these *** this work,we developed a unique automated template matching(ATM)cardiomyocyte beating model to achieve arrhythmic recognition at the single beat level with an interdigitated electrode impedance detection *** ATM model was established based on a rhythmic template with a data length that was dynamically adjusted to match the data length of the target beat by spline *** performance of the ATM model under long-term astemizole,droperidol,and sertindole treatment at different doses was *** results indicated that the ATM model based on a random rhythmic template of a signal segment obtained after astemizole treatment presented a higher recognition accuracy(100%for astemizole treatment and 99.14%for droperidol and sertindole treatment)than the ATM model based on arrhythmic *** believe this highly specific ATM method based on a cardiomyocyte beating model has the potential to be used for arrhythmia screening in the fields of cardiology and pharmacology.

关键词： channel template matching

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Tumor-on-a-chip:from bioinspired design to biomedical application

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Microsystems & Nanoengineering 2021年第3期7卷 27-49页

作者： Xingxing Liu jiaru fang Shuang Huang Xiaoxue Wu Xi Xie Ji Wang Fanmao Liu Meng Zhang Zhenwei Peng Ning Hu The First Affiliated Hospital of Sun Yat-Sen University State Key Laboratory of Optoelectr onic Materials and TechnologiesGuangdong Province Key Laboratory of Display Material and TechnologySchool of Electronics and Information TechnologySun Yat-Sen University510006 GuangzhouChina State Key Laboratory of Transducer Technology Chinese Academy of Sciences200050 ShanghaiChina

Cancer is one of the leading causes of human death,despite enormous efforts to explore cancer biology and develop anticancer *** main challenges in cancer research are establishing an efficient tumor microenvironment in vitro and exploring efficient means for screening anticancer drugs to reveal the nature of cancer and develop *** tumor microenvironment possesses human-specific biophysical and biochemical factors that are difficult to recapitulate in conventional in vitro planar cell models and in vivo animal ***,model limitations have hindered the translation of basic research findings to clinical *** this review,we introduce the recent progress in tumor-on-a-chip devices for cancer biology research,medicine assessment,and biomedical applications in *** emerging tumor-on-a-chip platforms integrating 3D cell culture,microfluidic tech no logy,and tissue engineeri ng have successfully mimicked the pivotal structural and functional characteristics of the in vivo tumor *** recent advances in tumor-on-a-chip platforms for cancer biology studies and biomedical applications are detailed and an a lyzed in this *** review should be valuable for further understanding the mechanisms of the tumor evolution process,screening anticancer drugs,and developing cancer therapies,and it addresses the challenges and potential opportunities in predicting drug screening and cancer treatment.

关键词： drugs integrating establishing

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Active Micro-Nano-Collaborative Bioelectronic Device for Advanced Electrophysiological Recording

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Nano-Micro Letters 2024年第7期16卷 244-264页

作者： Yuting Xiang Keda Shi Ying Li Jiajin Xue Zhicheng Tong Huiming Li Zhongjun Li Chong Teng jiaru fang Ning Hu Department of Chemistry Zhejiang-Israel Joint Laboratory of Self-Assembling Functional MaterialsZJU-Hangzhou Global Scientific and Technological Innovation CenterZhejiang UniversityHangzhou 310058People’s Republic of China School of Basic Medical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053People’s Republic of China General Surgery Department Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Children’s HealthHangzhou 310052People’s Republic of China Department of Lung Transplantation and General Thoracic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003People’s Republic of China Department of Orthopedics The Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322005People’s Republic of China School of Electronics and Information Technology Sun Yat-Sen UniversityGuangzhou 510006People’s Republic of China Department of Obstetrics and Gynecology The Tenth Affiliated HospitalSouthern Medical UniversityDongguan 523059People’s Republic of China Dongguan Key Laboratory of Major Diseases in Obstetrics and Gynecology Dongguan 523059People’s Republic of China

The development of precise and sensitive electrophysiological recording platforms holds the utmost importance for research in the fields of cardiology and *** recent years,active micro/nano-bioelectronic devices have undergone significant advancements,thereby facilitating the study of *** distinctive configuration and exceptional functionality of these active micro-nano-collaborative bioelectronic devices offer the potential for the recording of high-fidelity action potential signals on a large *** this paper,we review three-dimensional active nano-transistors and planar active micro-transistors in terms of their applications in electroexcitable cells,focusing on the evaluation of the effects of active micro/nano-bioelectronic devices on electrophysiological *** forward to the possibilities,challenges,and wide prospects of active micro-nano-devices,we expect to advance their progress to satisfy the demands of theoretical investigations and medical implementations within the domains of cardiology and neuroscience research.

关键词： Active micro/nano collaborative bioelectronic device Three-dimensional active nano-transistor Planar active microtransistor Electrophysiology

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心肌细胞电位传感器在海洋生物毒素检测中的研究

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传感技术学报 2016年第8期29卷 1127-1132页

作者：方佳如王琴黎洪波苏凯麒潘宇祥王平浙江大学生物传感器国家专业实验室生物医学工程教育部重点实验室生仪学院杭州310027

目前海洋毒素对人类健康的威胁越加严重,尤其是麻痹性毒素。提出了一种基于细胞电位传感器检测贝类的生物毒性的新方法,采用心肌细胞与微电子加工技术的微阵列芯片(MEA)相结合,构建了新型的心肌细胞电位传感器。通过心肌细胞与MEA芯片... 详细信息

目前海洋毒素对人类健康的威胁越加严重,尤其是麻痹性毒素。提出了一种基于细胞电位传感器检测贝类的生物毒性的新方法,采用心肌细胞与微电子加工技术的微阵列芯片(MEA)相结合,构建了新型的心肌细胞电位传感器。通过心肌细胞与MEA芯片的紧密耦合,实现了实时监测心肌细胞电位的变化,输出稳定和一致性好的胞外场电位信号。通过检测海洋中危害较大的石房蛤麻痹性毒素(STX),分析细胞的信号特征参数,实验结果表明,STX毒素对心肌细胞的电生理活动有明显的抑制作用,对脉冲幅值和脉冲频率有明显的浓度依赖性抑制作用。常规标准的生物毒性检测方法小鼠生物法的检出限为40 g/100 g,而心肌细胞电位传感器可检测的下限为1.004 ng/m L,检测范围为25 nmol/L^1 600 nmol/L,通过对比表明该方法具有较好的检出限且检测方法相对简单,易于规范化和标准化。通过进一步的完善,该方法在海洋毒素的快速检测应用方面具有良好的发展前景。

关键词：细胞传感器微电极阵列海洋毒素心肌细胞电位检测胞外场电位信号

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基于微纳传感调控技术的细胞胞内电子学的研究进展

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生命科学仪器 2022年第4期20卷 4-12页

作者：徐冬馨方佳如胡宁中山大学电子与信息工程学院广州510006 浙江大学杭州国际科创中心化学系杭州310058

建立可靠的电生理记录平台对于心脏病学和神经科学的研究至关重要。近十年来,基于微纳传感调控技术的器件被开发用于电生理平台的搭建,其独特的形貌优势和新颖的加工方式,使得高通量、高保真的电信号记录有望被实现。本综述分别从微纳... 详细信息

建立可靠的电生理记录平台对于心脏病学和神经科学的研究至关重要。近十年来,基于微纳传感调控技术的器件被开发用于电生理平台的搭建,其独特的形貌优势和新颖的加工方式,使得高通量、高保真的电信号记录有望被实现。本综述分别从微纳传感调控器件的材料、结构、加工和穿膜策略等方面进行了总结,并对其在心肌细胞和神经细胞电生理检测上的应用进行了分析。在微纳传感调控技术所面临的机遇挑战下,对其广阔的发展前景进行了展望,希望借助其快速发展来推动更高层次的电生理平台搭建,以满足实验研究以及临床应用的需求。

关键词：微纳传感调控技术细胞内电生理电兴奋细胞纳米生物界面

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基于鼠精细胞的味觉阻抗传感器用于苦味物质检测的研究

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传感技术学报 2016年第12期29卷 1785-1790页

作者：田玉兰苏凯麒邱先鑫方佳如秦臻李蓉王平浙江大学生物传感器国家专业实验室生物医学工程教育部重点实验室生仪学院杭州310027

本文设计了一种基于雄鼠精细胞的细胞阻抗传感器用于苦味物质的特异性检测。雄鼠精细胞内含有大量T2Rs受体(G蛋白偶联受体)可以对苦味物质产生特异性响应,苦味受体被苦味物质激活后产生的响应会引起细胞形态骨架的变化,从而可以被细胞... 详细信息

本文设计了一种基于雄鼠精细胞的细胞阻抗传感器用于苦味物质的特异性检测。雄鼠精细胞内含有大量T2Rs受体(G蛋白偶联受体)可以对苦味物质产生特异性响应,苦味受体被苦味物质激活后产生的响应会引起细胞形态骨架的变化,从而可以被细胞阻抗传感器检测。本文探索了实验的最佳细胞密度,检测了苯硫脲和奎宁两种常见的苦味物质的响应,苯硫脲检测范围为10μmol/L^200μmol/L,检出限为4μmol/L;奎宁检测范围为62.5μmol/L^1 000μmol/L,检出限为40μmol/L。传感器阻抗值增量与苦味物质浓度呈一定的线性相关性。此外,论文对该味觉传感器的特异性进行了测试分析,表明了基于细胞传感器的检测方法为代替动物和人的苦味测试提供了一种可能的新的手段。

关键词：苦味检测细胞阻抗传感器精细胞

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木蝴蝶内生真菌分离鉴定及促生功能初步研究

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云南农业大学学报（自然科学版） 2023年第5期38卷 878-885页

作者：许娟娟房佳茹周士家李伟孙正海西南林业大学园林园艺学院云南省面向南亚东南亚经济林全产业链联合研发中心云南省观赏草集成利用国际联合研发中心云南省高效经济林培育示范型国际科技合作基地云南昆明650224 云南吉成园林科技股份有限公司云南弥勒652339

【目的】解析木蝴蝶[Oroxylum indicum(L.)Vent.]内生真菌的种类及其促生功能。【方法】以木蝴蝶树皮、种子、果皮和叶片为材料分离鉴定内生真菌,依据生理指标对分离的内生真菌促生活性进行分析;选取部分代表性内生真菌转接到黄豆植株... 详细信息

【目的】解析木蝴蝶[Oroxylum indicum(L.)Vent.]内生真菌的种类及其促生功能。【方法】以木蝴蝶树皮、种子、果皮和叶片为材料分离鉴定内生真菌,依据生理指标对分离的内生真菌促生活性进行分析;选取部分代表性内生真菌转接到黄豆植株进行培养,以黄豆产量指标验证其实际促生效果。【结果】从木蝴蝶树皮、种子、果皮和叶片中分离得到50株内生真菌,隶属于13属23种,其中果皮部位分离菌株最多,炭疽属(Colletotrichum)为优势菌属;9株代表性真菌均具有产吲哚乙酸和固氮的能力,其中6株真菌还具有溶磷和解钾特性。转接栽培试验结果表明:9株菌处理后黄豆果荚数增加5%~75%,产量提高37%~231%;其中壳青霉(Penicillium crustosum)促生效果最好。【结论】本研究首次开展了木蝴蝶内生真菌的分离研究,并对其功能进行了验证,其中壳青霉促生效果最好,转接黄豆后可提高其果荚数和产量,具有开发为微生物菌肥的潜力。

关键词：木蝴蝶内生真菌纯培养分离促生功能

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