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Science China(Life Sciences) 2019年第1期62卷 112-118页

作者： Ziru Jiang Li Jin Weihui Shi Ji Xi Yulian Hu Xinmei Liu jianzhong sheng Guolian Ding Hefeng Huang The International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University Institute of Embryo-Fetal Original Adult Disease Shanghai Key Laboratory for Reproductive Medicine School of Medicine Shanghai Jiao Tong University Department of Pathology and Pathophysiology School of Medicine Zhejiang University

To investigate the effect of basal hormone levels including follicle stimulating hormone(FSH) and estradiol(E2) and age on the pregnancy outcomes in women undergoing in vitro fertilization and embryo transfer(IVF-ET), we designed a retrospective cohort analysis and collected information of patients undergoing IVF between January 2014 and April 2017. We found that young women had much better pregnancy outcomes than older women regardless of the hormone levels. The pregnancy outcomes declined in young women with high basal E2, even though they had normal FSH. Older women with higher FSH had a worse outcome of pregnancy, especially with increased E2 level. The results suggest that a combination of FSH, E2 and age could effectively predict the pregnancy outcome for women undergoing IVF-ET, and we should encourage infertile women with diminished ovarian reserve, especially young infertile women, to try the assisted reproduction as early as possible. After 40-yearold, the cost-benefit ratio should be considered according to the ovarian function.

关键词： follicle stimulating hormone estradiol age pregnancy outcome assisted reproduction

Mechanism underlying the retarded nuclear translocation of androgen receptor splice variants

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Science China(Life Sciences) 2019年第2期62卷 257-267页

作者： Ye Liu Yinyu Wang Fangfang Wang Jiexue Pan Jingjing Xu Jingyi Li Chengliang Zhou Guolian Ding Yanting Wu Xinmei Liu jianzhong sheng Hefeng Huang The International Peace Maternity and Child Health Hospital Shanghai Jiao Tong University School of Medicine The Key Laboratory of Reproductive Genetics (Ministry of Education) Zhejiang University Department of Pathophysiology Zhejiang University School of Medicine

As shown in our previous study, two alternatively spliced androgen receptor(AR) variants, which are exclusively expressed in the granulosa cells of patients with polycystic ovary syndrome, exhibit retarded nuclear translocation compared with wild-type AR. However, researchers have not yet determined whether these abnormalities correlate with heat shock protein 90(HSP90)and importin α(the former is a generally accepted co-chaperone of AR, and the latter is a component of classical nuclear import complexes). Here, these two variants were mainly retained in cytoplasm with HSP90 and importin α in the presence of dihydrotestosterone(DHT), and their levels in nucleus were significantly reduced, according to the immunofluorescence staining. The binding affinity of two AR variants for importin α was consistently decreased, while it was increased in WT-AR following DHT stimulation, leading to reduced nuclear import, particularly for the insertion-AR(Ins-AR). However, the binding affinities of two AR variants for HSP90 were increased in the absence of DHT compared with WT-AR, which functioned to maintain spatial structural stability, particularly for the deletion-AR(Del-AR). Therefore, the retarded nuclear translocation of two AR variants is associated with HSP90 and importin α, and the abnormal binding affinities for them play critical roles in this process.

关键词： androgen receptor splice variants nuclear translocation heat shock protein 90 importin α polycystic ovary syndrome

Intrauterine hyperglycemia impairs endometrial receptivity via up-regulating SGK1 in diabetes

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Science China(Life Sciences) 2022年第8期65卷 1578-1589页

作者： Haiyan Xu Jingyi Li Luyang Jin Dan Zhang Bin Chen Xinmei Liu Xianhua Lin Yiting Huang Zhanghong Ke Juan Liu Lin Gao jianzhong sheng Hefeng Huang Reproductive Medicine Center Ningbo First HospitalNingbo 315100China Key Laboratory of Reproductive Genetics Ministry of Education(Zhejiang University)Hangzhou 310058China Department of Reproductive Endocrinology Women’s HospitalZhejiang University School of MedicineHangzhou 310006China Department of Pathology&Pathophysiology Zhejiang University School of MedicineHangzhou 310058China Shanghai Key Laboratory of Embryo Original Diseases Shanghai 200030China

Diabetes is a complex metabolic disorder which can adversely affect reproductive function. SGK1 is found to be up-regulated in multiple tissues of diabetic patients. However, the effects of diabetes on endometrial SGK1 expression and endometrial receptivity remain unknown. In this study, we established a streptozotocin-induced diabetic mouse model and observed reduced implantation sites, retarded development of pinopodes, increased SGK1, and aberrant expression of LIF and MUC1 in the endometrial epithelium. We injected the uterine lumen of normal mice with high-glucose solution and cultured endometrial cells in high-glucose medium to mimic intrauterine hyperglycemia. Both studies provided compelling evidence that hyperglycemia could lead to diminished embryo implantation and dysregulated SGK1, LIF and MUC1. Additionally, through over-expression of SGK1 in vivo and in vitro, we found that enhanced SGK1 also decreased LIF expression, increased MUC1 expression, and attenuated embryo implantation rate. We further identified that hyperglycemia-activated SMAD2/3 might be responsible for the enhancement of SGK1 and verified directly the interaction between SMAD3 and corresponding SMAD binding elements within SGK1 promoter. Taken together, our study confirmed the association between diabetes-related hyperglycemia and endometrial receptivity defects. Hyperglycemia-induced SGK1 has a tremendous role in this pathological process, rendering it as an attractive therapeutic target for diabetes-related reproductive disorders.

关键词： diabetes endometrial receptivity hyperglycemia serum/glucocorticoid-regulated kinase

A novel preimplantation genetic testing strategy for a subtelomeric genetic disorder:A case study

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Genes & Diseases 2024年第4期11卷 13-15页

作者： Songchang Chen Li Wanng Luting Chen Weihui Shi Junyu Zhang Yuting Hu Yinyu Wang Li Jin jianzhong sheng Feng Zhang Yanting Wu Hefeng Huang Chenming Xu Obstetrics and Gynecology Hospital Institute of Reproduction and DevelopmentFudan UniversityShanghai 200090China The International Peace Maternity and Child Health Hospital School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Research Units of Embryo Original Diseases Chinese Academy of Medical SciencesShanghai 200030China Key Laboratory of Reproductive Genetics(Ministry of Education) Department of Reproductive EndocrinologyWomen's HospitalZhejiang University School of MedicineHangzhouZhejiang 310058China Shanghai Key Laboratory of Embryo Original Diseases Shanghai 200030China Shanghai First Maternity and Infant Hospital Tongji University School of MedicineShanghai 200030China

Preimplantation genetic testing(PGT)is a precise and effective technique for detecting inherited pathogenic mutations to prevent birth ***,there are few reports on PGT for pseudo-autosomal genetic diseases.

关键词： implantation testing precise

Decreased decidual epithelial sodium channel(ENaC) alpha and gamma subunits in recurrent pregnancy loss(RPL)

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Science China(Life Sciences) 2019年第7期62卷 988-990页

作者： Tiantian Yu Chun Feng Pingping Lv Gufeng Xu Lihua Hong Yimeng Xiong Xiaoyan Guo Tong Li jianzhong sheng Hefeng Huang Xinmei Liu International Peace Maternal and Child Health Hospital Shanghai Jiao Tong UniversitySchool of MedicineShanghai 200030China The Second Affiliated Hospital School of MedicineZhejiang UniversityHangzhou 310009China Key Laboratory of Reproductive Genetics Ministry of EducationZhejiang UniversityHangzhou 310006China Department of Reproductive Endocrinology Women's HospitalSchool of MedicineZhejiang UniversityHangzhou 310006China Department of Reproductive Endocrinology Zhejiang Provincial People's HospitalHangzhou Medical CollegeHangzhou 310014China Department of Pathology and Pathophysiology School of MedicineZhejiang UniversityHangzhou 310063China Institute of Embryo-Fetal Original Adult Diseases School of MedicineShanghai Jiao Tong UniversityShanghai 200030China

Dear Editor,Recurrent pregnancy loss (RPL) in early pregnancy is a devastating problem for couples who want to become parents and a difficult challenge for their *** is also referred to as recurrent miscarriage or habitual *** is defined as two or more consecutive clinical miscarriages (CMs) before 20 weeks of gestation (Practice Committee of the American Society for Reproductive Medicine,2012).

关键词： Editor pregnancy Medicine

An alternative splicing variant of mineralocorticoid receptor discovered in preeclampsia tissues and its effect on endothelial dysfunction

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Science China(Life Sciences) 2020年第3期63卷 388-400页

作者： Mengxi Guo Chengliang Zhou Gufeng Xu Lin Tang Yechun Ruan Ying Yu Xianhua Lin Dandan Wu Hao Chen Priscilla Yu Luyang Jin Yinyu Wang Yimei Wu Kamran Ullah Tanzil Ur Rahman Xinmei Liu jianzhong sheng Hsiao-Chang Chan Hefeng Huang Women’s Hospital Zhejiang University School of MedicineHangzhou 310058China International Peace Maternal and Child Health Hospital Shanghai Jiao Tong University School of MedicineShanghai 200030China Epithelial Cell Biology Research Center Faculty of MedicineSchool of Biomedical SciencesThe Chinese University of Hong KongHong KongChina Key Laboratory of Reproductive Genetics Ministry of Education(Zhejiang University)Hangzhou 310058China Department of Pathology and Pathophysiology Zhejiang University School of MedicineHangzhou 310058China Shanghai Key Laboratory of Embryo Original Disease Shanghai 20030China

The pathophysiology of preeclampsia(PE)remains *** spiral artery remodeling dysfunction and PE offspring cardiovascular future development has been a worldwide *** collected placental and umbilical artery samples from normotensive and PE *** receptor(MR)and its alternative splicing variant(ASV)expression and their biological effects on PE were *** MR ASV was found to be highly expressed in all PE samples and slightly expressed in about half of the normotensive samples(umbilical artery,~57.58%;placenta,~36.84%).The MR ASV expression was positively associated with blood pressure in both *** MR ASV protein changed the aldosterone-induced expression pattern of MR target genes related to ion exchanges and cell signaling *** MR ASV can also impair the proliferation,migration,and tube formation ability of endothelial *** findings indicate that MR ASV in PE placenta plays a pathogenic role in PE pathophysiology,especially in endothelial dysfunction,and the existence of the MR ASV in PE umbilical artery provides a new direction in the study of PE offspring with increased risk of cardiovascular diseases.

关键词： preeclampsia mineralocorticoid receptor alternative splicing variant offspring endothelial dysfunction

妊娠期不良暴露对子代骨骼肌功能影响的研究进展

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浙江大学学报（医学版） 2024年第3期53卷 271-279页

作者：刘睿盛建中黄荷凤浙江大学医学院附属第四医院浙江大学“一带一路”国际医学院国际健康医学研究院生殖医学中心浙江义乌322000 浙江大学医学院生殖遗传教育部重点实验室浙江杭州310006 上海市胚胎源性疾病重点实验室中国医学科学院胚胎源性疾病研究创新单元上海200030 复旦大学附属妇产科医院生殖与发育研究所上海200030

骨骼肌作为机体最重要的肌肉之一,在维持机体代谢功能、能量稳态、运动功能和内分泌功能中发挥重要作用。妊娠期是人体骨骼肌生成和发育的关键时期。妊娠期母体遭受到的不良环境暴露会对子代骨骼肌的健康产生不同的影响。妊娠期肥胖会... 详细信息

骨骼肌作为机体最重要的肌肉之一,在维持机体代谢功能、能量稳态、运动功能和内分泌功能中发挥重要作用。妊娠期是人体骨骼肌生成和发育的关键时期。妊娠期母体遭受到的不良环境暴露会对子代骨骼肌的健康产生不同的影响。妊娠期肥胖会通过影响胎儿骨骼肌代谢及炎症相关通路的改变介导子代骨骼肌中脂质形成;妊娠期高糖高脂等不良饮食习惯会影响子代骨骼肌线粒体的自噬功能,降低子代骨骼肌质量;妊娠期营养缺乏会通过影响子代骨骼肌的表观遗传学修饰影响其发育;妊娠糖尿病可能通过上调子代骨骼肌中微RNA(miR)-15a和miR-15b水平影响其功能;妊娠期环境内分泌干扰物暴露可能通过干扰子代骨骼肌胰岛素受体相关信号通路损害骨骼肌功能。本文综述了临床和模式动物研究中妊娠期母体不良暴露对子代骨骼肌功能的影响及可能机制,以期为子代骨骼肌出生缺陷的防治提供科学依据。

关键词：孕期不良暴露子代骨骼肌发育源性疾病综述

Development of Water Resources Management

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Development of Water Resources Management

作者： Sun Jichang Zu Leiming Zhang Yanming Zhang Wenjie Cui jianzhong sheng Jinbao Sun Qingqiao

As a branch of water resources study,water resources management is a new intersectional subject which involves multiple *** the development of water resources and scientific technology,the,scope of water resources has...

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基层央行微服务容器技术研究与应用探索

金融科技时代 2019年第8期27卷 57-59页

作者：盛建忠中国人民银行南通市中心支行

IT应用与项目建设向分布式架构转型是人民银行科技部门今后的重要工作之一。面对快速发展的分布式技术,本文结合基层央行的实际应用需求,对微服务容器技术在基层央行的应用场景进行了研究,并尝试通过DaoCloud这一容器管理和运维工具来搭... 详细信息

IT应用与项目建设向分布式架构转型是人民银行科技部门今后的重要工作之一。面对快速发展的分布式技术,本文结合基层央行的实际应用需求,对微服务容器技术在基层央行的应用场景进行了研究,并尝试通过DaoCloud这一容器管理和运维工具来搭建Docker服务集群,以提高业务系统运行的连续性与保障性,为业务应用系统建设向分布式架构转型提供了有效的探索路径。

关键词：分布式架构微服务容器技术应用场景

Gestational Diabetes Mellitus Induces Altered Protein Expression Profile of the Umbilical Arteries as Revealed by iTRAQ-based Quantitative Proteomics.

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Gestational Diabetes Mellitus Induces Altered Protein Expres...