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Identification of S-RNase genotype and analysis of its origin and evolutionary patterns in Malus plants

Journal of Integrative Agriculture 2024年第4期23卷 1205-1221页

作者： Zhao Liu Yuan Gao Kun Wang jianrong feng Simiao Sun Xiang Lu Lin Wang Wen Tian Guangyi Wang Zichen Li Qingshan Li Lianwen Li Dajiang Wang Research Institute of Pomology Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources UtilizationMinistry of Agriculture and Rural AffairsXingcheng 125100China College of Agriculture Shihezi University/Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction CorpsShihezi 832000China

Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibility in Malus *** this experiment,88 Malus germplasm resources,such as Aihuahong,Xishuhaitang,and Reguanzi,were used as *** gene-specific primer combinations were used in the genotype *** amplification using leaf DNA produced a single S-RNase gene fragment in all *** results revealed that 70 of the identified materials obtained a complete S-RNase genotype,while only one S-RNase gene was found in 18 of *** homology comparison and analysis,13 S-RNase genotypes were obtained:S_(1)S_(2)(Aihuahong,etc.),S_(1)S_(28)(Xixian Haitang,etc.),S_(1)S_(51)(Hebei Pingdinghaitang),S_(1)S_(3)(Xiangyangcun Daguo,etc.),S_(2)S_(3)(Zhaiyehaitang,etc.),S_(3)S_(51)(Xishan 1),S_(3)S_(28)(Huangselihaerde,etc.),S_(2)S_(28)(Honghaitang,etc.),S_(4)S_(28)(Bo 11),S_(7)S_(28)(Jiuquan Shaguo),S_(10)S_e(Dongchengguan 13),S_(10)S_(21)(Dongxiangjiao)and S_(3)S_(51)(Xiongyue Haitang).Simultaneously,the frequency of the S gene in the tested materials was *** findings revealed that different S genes had varying frequencies in Malus resources,as well as varying frequencies between intraspecific and interspecific.S_(3) had the highest frequency of 68.18%,followed by S_(1)(42.04%).In addition,the phylogenetic tree and origin evolution analysis revealed that the S gene differentiation was completed prior to the formation of various apple species,that cultivated species also evolved new S genes,and that the S_(50) gene is the oldest S allele in Malus *** S_(1),S_(29),and S_(33) genes in apple-cultivated species,on the other hand,may have originated in ***,***,and ***,*** addition to ***,*** and *** may have also played a role in the origin and evolution of some Chine

关键词： Malus S-RNase genotype self-incompatibility origin and evolution

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Multi-Objective Optimization of VBHF in Deep Drawing Based on the Improved QO-Jaya Algorithm

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Chinese Journal of Mechanical Engineering 2024年第1期37卷 189-202页

作者： Xiangyu Jiang Zhaoxi Hong Yixiong feng jianrong Tan State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027China Key Laboratory of Advanced Manufacturing Technology of Zhejiang Province Zhejiang UniversityHangzhou 310027China Ningbo Innovation Center Zhejiang UniversityNingbo 315100China

Blank holder force(BHF)is a crucial parameter in deep drawing,having close relation with the forming quality of sheet ***,there are different BHFs maintaining the best forming effect in different stages of deep *** variable blank holder force(VBHF)varying with the drawing stage can overcome this problem at an *** optimization of VBHF is to determine the optimal BHF in every deep drawing *** this paper,a new heuristic optimization algorithm named Jaya is introduced to solve the optimization *** improved“Quasi-oppositional”strategy is added to Jaya algorithm for improving population ***,an innovated stop criterion is added for better ***,the quality evaluation criteria for wrinkling and tearing are ***,the Kriging models are developed to approximate and quantify the relation between VBHF and forming defects under random ***,the optimization models are established and solved by the improved QO-Jaya algorithm.A VBHF optimization example of component with complicated shape and thin wall is studied to prove the effectiveness of the improved Jaya *** optimization results are compared with that obtained by other algorithms based on the TOPSIS method.

关键词： Variable blank holder force Multi-objective optimization QO-Jaya algorithm Algorithm stop criterion

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苹果重要性状全基因组关联分析研究进展

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园艺学报 2024年第7期51卷 1565-1579页

作者：田雯李子琛王霖王大江王昆孙思邈王广艺刘昭路翔冯建荣高源石河子大学农学院特色果树栽培生理与种质资源利用兵团重点实验室新疆石河子832000 中国农业科学院果树研究所农业农村部园艺作物种质资源利用重点实验室辽宁兴城125100

简要介绍了全基因组关联分析(genome-wide association study,GWAS)的基本原理、分析流程及优势,重点阐述了苹果物候期、外观和内在品质、抗生物和非生物胁迫等重要性状相关基因鉴定研究取得的新进展,提出了当前研究的局限性并预测了前... 详细信息

简要介绍了全基因组关联分析(genome-wide association study,GWAS)的基本原理、分析流程及优势,重点阐述了苹果物候期、外观和内在品质、抗生物和非生物胁迫等重要性状相关基因鉴定研究取得的新进展,提出了当前研究的局限性并预测了前景,旨在为培育苹果突破性品种提供理论依据和参考。

关键词：苹果全基因组关联分析遗传育种研究进展

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Nitric Oxide Regulates Mitochondrial Fatty Acids and Promotes CBF Expression of Peach Fruit during Cold Storage

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Phyton-International Journal of Experimental Botany 2022年第2期91卷 409-427页

作者： Xiaoshan Guo Dandan Huang Siyu Wen Yang Bai Shuhua Zhu jianrong feng Department of Horticulture College of AgricultureShihezi UniversityShihezi832000China College of Chemistry and Material Science Shandong Agricultural UniversityTaian271018China

Low-temperature storage is convenient for postharvest preservation of peach fruit,but peach fruit is sensitive to cold damage,which lowers its *** oxide(NO)has the potential to improve the bitter resistance of peach *** this work,peach fruit was treated with 15μmol L^(−1)NO and 5μmol L^(−1)c-PTIO[2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxo-3-oxide],to study changes in mitochondrial fatty acids and expression of the C-repeat binding factor(CBF).The results showed that 15μmol L^(−1)exogenous NO significantly maintained fruit quality,reduced peroxidation of mitochondrial fatty acids,increased the activities of the antioxidants superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbic acid peroxidase(APX),and reduced the content of hydrogen peroxide(H_(2)O_(2)).Meanwhile,NO treatment suppressed the increase in browning index and ion leakage rate,increased the activity of S-nitrosoglutathione reductase(GSNOR),the contents of S-nitrosothiols(SNOs),and the ratios of mitochondrial NAD^(+)/NADH and NADP^(+)/NADPH,increased the expression levels of PpCBF1/5/***,the expression levels of PpCBF2/3/4 were not significantly regulated by exogenous *** treated with c-PTIO showed opposite effects to those treated with exogenous *** results suggest that exogenous NO can improve antioxidant capacity,preserve mitochondrial fatty acids,and upregulate the expression of PpCBF1/5/6 to alleviate cold tolerance and maintain the peach quality during storage.

关键词： Peach cold storage nitric oxide mitochondrial fatty acid CBF

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Synaptonemal complex protein 2 （SYCP2） mediates the association of the centromere with the synaptonemal complex

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Protein & Cell 2017年第7期8卷 538-543页

作者： feng, jianrong Fu, Shijuan Cao, Xuan Wu, Hao Lu, Jing Zeng, Ming Liu, Lin Yang, Xue Shen, Yuequan Nankai Univ State Key Lab Med Chem Biol Tianjin 300071 Peoples R China Nankai Univ Coll Life Sci Tianjin 300071 Peoples R China Synerget Innovat Ctr Chem Sci & Engn Tianjin 300071 Peoples R China

Dear Editor,In neady all diploid eukaryotes, meiosis is the key phase in sexual reproduction. The typical process is completed by a round of DNA replication followed by two consecutive rounds of chromosome cell division （Ma et al., 2014; Page and Hawley, 2004）. Homologous chromosome pairing and seg- regation occur in the first round of meiosis and is unique to mitosis. The second round of meiosis bears a similarity to the separation of sister chromatids.

关键词： Down Syndrome Synaptonemal Complex Synaptonemal Complex Formation Diplotene Stage Synaptonemal Complex Structure

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新疆南疆5个杏品种开花的生物学特性

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中国果树 2024年第3期 48-53页

作者：康江芳代英姿杨志峰唐章虎王绍鹏张大海冯建荣石河子大学农学院特色果树栽培生理与种质资源利用兵团重点实验室新疆832000 新疆农业科学院园艺作物研究所

以新疆南疆库买提等5个杏品种为试材,对其花期、花粉生活力、雌蕊败育率、柱头可授性及坐果率进行了调查,为该地区杏栽培授粉品种配置及高产稳产提供参考。结果表明:5个品种中,大白油杏花芽萌动期最早,吾候其和大白油杏始花期均最早,吾... 详细信息

以新疆南疆库买提等5个杏品种为试材,对其花期、花粉生活力、雌蕊败育率、柱头可授性及坐果率进行了调查,为该地区杏栽培授粉品种配置及高产稳产提供参考。结果表明:5个品种中,大白油杏花芽萌动期最早,吾候其和大白油杏始花期均最早,吾候其盛花期最早;同时品种间花芽萌动期变异系数最大,为51.35%,末花期变异系数最小,为5.66%。库买提花粉萌发率最高,与晚熟佳娜丽存在显著差异。雌蕊败育率在不同品种间差异显著,雌蕊败育率最高的是大白油杏,最低的是库买提。柱头最佳授粉时期具有差异,其中,库买提、晚熟佳娜丽、吾候其分别在花后第2 d和第3 d柱头达极强可授性。坐果率与雌蕊败育率呈极显著负相关关系,与花粉生活力呈正相关关系。5个杏品种花期持续6~9 d,吾候其、库买提的花粉生活力、坐果率均较强;大白油杏、晚熟佳娜丽雌蕊败育率均较高;库买提、晚熟佳娜丽、吾候其的柱头在最佳授粉时期均表现出极强的可授性。

关键词：杏花期雌蕊败育柱头可授性花粉生活力

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苹果响应盐胁迫相关miRNA的深度测序数据集

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农业大数据学报 2024年第1期6卷 14-23页

作者：刘昭高源王昆孙思邈代瑛姿路翔田雯王大江冯建荣石河子大学农学院/特色果树栽培生理与种质资源利用兵团重点实验室新疆石河子832000 中国农业科学院果树研究所/农业农村部园艺作物种质资源利用重点实验室辽宁兴城125100

土壤盐渍化已成为限制农作物产量与质量的重要因素之一,影响着全世界范围内的农作物产量。苹果作为一种重要的水果作物,盐胁迫的危害及耐盐砧木的缺乏已成为威胁现代苹果产业健康可持续发展的重要问题。因此,研究苹果对盐胁迫的反应和... 详细信息

土壤盐渍化已成为限制农作物产量与质量的重要因素之一,影响着全世界范围内的农作物产量。苹果作为一种重要的水果作物,盐胁迫的危害及耐盐砧木的缺乏已成为威胁现代苹果产业健康可持续发展的重要问题。因此,研究苹果对盐胁迫的反应和适应具有重要的现实意义和紧迫性。本研究分别以0%、0.2%和0.6%NaCl处理0、4、8和12天后的珠美海棠实生苗为试验材料,从叶片及根系中提取总RNA,构建RNA-seq文库,共鉴定获得575个miRNA,包括315个已知mdm-miRNA和260个新miRNA。此外,Differential Expression(DE) miRNA对盐胁迫反应具有组织特异性表达模式。同时提供珠美海棠miRNA深度测序的数据集,结合生物信息学方法对原始数据质量控制,获得高质量序列并利用BLAST软件将预测靶基因序列与NR、Swiss-Prot、Pfam等数据库比对,获得响应盐胁迫相关基因的注释信息。GO分析表明与盐胁迫有关miRNA及靶基因主要参与生物过程中对刺激的反应。本研究为珠美海棠的利用提供理论数据基础,并为耐盐砧木的选育提供有力支持。

关键词：苹果盐胁迫 miRNA 深度测序生物信息学

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Control of temperature dependent viscosity for manufacturing of Bi-doped active fiber

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International Journal of Extreme Manufacturing 2024年第3期6卷 522-530页

作者： Rui Duan Jingfei Chen Hao Ke Tianxia Wei Ke Zhang Xueliang Li Xu feng Qiuju Zheng Zhixue He jianrong Qiu Shifeng Zhou State Key Laboratory of Luminescent Materials and Devices School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou 510640People’s Republic of China Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and DevicesGuangzhou 510640People’s Republic of China School of Materials Science and Engineering Qilu University of TechnologyJinanPeople’s Republic of China Peng Cheng Laboratory Shenzhen 518000People’s Republic of China College of Optical Science and Engineering State Key Laboratory of Modern Optical InstrumentationZhejiang UniversityHangzhou 310027People’s Republic of China

Bi-activated photonic materials are promising for various applications in high-capacity telecommunication,tunable laser,and advanced bioimaging and *** various Bi-doped material candidates have been explored,manufacturing of Bi heavily doped fiber with excellent optical activity remains a long-standing ***,a novel viscosity evolutional behavior mediated strategy for manufacturing of Bi-doped active fiber with high dopant solubility is *** intrinsic relation among the evolution of Bi,reaction temperature and viscosity of the glass system is ***,the effective avenue to prevent the undesired deactivation of Bi during fiber drawing by tuning the temperature dependent viscosity evolution is *** applying the strategy,for the first time we demonstrate the success in fabrication of heavily doped Bi active ***,the principal fiber amplifier device is constructed and broadband optical signal amplification is *** findings indicate the effectiveness of the proposed temperature dependent viscosity mediated strategy for developing novel photonic active fiber,and they also demonstrate the great potential for application in the next-generation high-capacity telecommunication system.

关键词： infrared luminescence active fiber optical amplification

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联会复合体蛋白SYCP2 C-末端的表达、结晶及晶体初步分析

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南开大学学报（自然科学版） 2017年第6期50卷 43-47页

作者：冯建荣南开大学生命科学学院天津300071

联会复合体蛋白2(SYCP2)作为减数分裂过程中表达的特异性蛋白,是联会复合体轴向组分之一.许多研究表明SYCP2对联会复合体正确组装/去组装可能起着重要作用,尤其是C末端,但具体机制尚不清楚.对鼠源的SYCP2-CTR进行了表达、纯化并用坐滴... 详细信息

联会复合体蛋白2(SYCP2)作为减数分裂过程中表达的特异性蛋白,是联会复合体轴向组分之一.许多研究表明SYCP2对联会复合体正确组装/去组装可能起着重要作用,尤其是C末端,但具体机制尚不清楚.对鼠源的SYCP2-CTR进行了表达、纯化并用坐滴气相扩散法进行了结晶和优化.在日本PEK收集了1套分辨率为0.4 nm的数据.晶体的空间群为C2,晶胞参数a=16.325 1,b=7.426,7 c=14.823 nm;α=γ=90?,β=119.464?.1个不对称单位4个分子,其溶剂量约为46%.

关键词： SYCP2 联会复合体减数分裂结晶

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新疆中亚生态群杏品种果实与叶片形态特征相关性

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新疆农业科学 2020年第1期57卷 120-126页

作者：徐乐冯建荣章世奎石河子大学农学院新疆石河子832003 新疆农业科学院国际科技合作交流处乌鲁木齐830091 新疆农业科学院轮台果树资源圃新疆轮台841600

【目的】研究新疆中亚生态群杏品种果实和叶片的形态特性及相关性,为新品种选育提供理论依据。【方法】以隶属于该生态群的80个新疆杏品种为研究对象,测定其在果实成熟期的叶面积、叶片长、叶片宽、叶周长、单果重、果实纵横侧径,分析... 详细信息

【目的】研究新疆中亚生态群杏品种果实和叶片的形态特性及相关性,为新品种选育提供理论依据。【方法】以隶属于该生态群的80个新疆杏品种为研究对象,测定其在果实成熟期的叶面积、叶片长、叶片宽、叶周长、单果重、果实纵横侧径,分析这些指标的分布特性和彼此间的相关性。【结果】80个新疆中亚生态群杏品种中,77.5%的杏品种叶面积分布在19.8~30.3 cm 2,最大叶面积为44.18 cm^2,最小叶面积为12.82 cm^2,叶面积整体偏小;86.3%杏品种叶周长分布在16.7~22.7 cm,最大叶周长为24.44 cm,最小叶周长为13.66 cm;63.8%杏品种单果重分布在16.1~32.1 g FW,最大单果重为48.07 g FW,最小单果重为12.13 g FW,果实整体偏小。叶面积与叶片宽之间相关性最高(0.910**),叶周长与叶片长之间相关性最高(0.870**),单果重与果实横径之间相关性最高(0.913**)。【结论】叶面积和叶周长与果实的单果重之间极显著正相关,可作为预测果实单果重的重要指标。

关键词：杏中亚生态群果实叶片形态特征

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