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Journal of Genetics and Genomics 2024年第2期51卷 159-183页

作者： Yuxi Chen Xiao Luo Rui Kang Kaixin Cui jianping ou Xiya Zhang Puping Liang MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen UniversitySun Yat-sen UniversityGuangzhouGuangdong 510630China

Osteoarthritis(OA)is one of the most common degenerative joint diseases worldwide,causing pain,disability,and decreased quality of life.The balance between regeneration and inflammation-induced degradation results in multiple etiologies and complex pathogenesis of OA.Currently,there is a lack of effective therapeutic strategies for OA treatment.With the development of CRISPR-based genome,epigenome,and RNA editing tools,OA treatment has been improved by targeting genetic risk factors,activating chondrogenic elements,and modulating inflammatory regulators.Supported by cell therapy and in vivo delivery vectors,genome,epigenome,and RNA editing tools may provide a promising approach for personalized OA therapy.This review summarizes CRISPR-based genome,epigenome,and RNA editing tools that can be applied to the treatment of OA and provides insights into the development of CRISPR-based therapeutics for OA treatment.Moreover,in-depth evaluations of the efficacy and safety of these tools in human OA treatment are needed.

关键词： Osteoarthritis Genome editing Epigenone editing RNA editing

Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing

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Protein & Cell 2022年第5期13卷 316-335页

作者： Xiya Zhang Tao Li jianping ou Junjiu Huang Puping Liang Center for Reproductive Medicine the Third Affiliated Hospital of Sun Yat-sen UniversitySun Yat-sen UniversityGuangzhou 510630China MOE Key Laboratory of Gene Function and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Reproductive Medicine of Guangdong Province the First Affiliated Hospital and School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Recent advances in genome editing,especially CRISPR-Cas nucleases,have revolutionized both laboratory research and clinical therapeutics.CRISPR-Cas nucleases,together with the DNA damage repair pathway in cells,enable both genetic diversification by classical non-homologous end joining(c-NHEJ)and precise genome modification by homology-based repair(HBR).Genome editing in zygotes is a convenient way to edit the germline,paving the way for animal disease model generation,as well as human embryo genome editing therapy for some life-threatening and incurable diseases.HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template.Here,we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor.Moreover,we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos.Finally,we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.

关键词： homology-based repair(HBR) genome editing disease modeling embryo precision medicine

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军事治理现代化的战略领导力

中国领导科学 2024年第2期 41-50页

作者：欧建平中国人民解放军国防大学原军队建设研究所

军事治理的现代化,首先应当是战略领导力的现代化。现代化的军事治理是一个系统工程,战略层面的军事治理,对于整个系统的军事治理具有决定性意义。深化国防和军队改革,对人民军队战略领导、战略指挥、战略管理体系进行了全新设计,形成... 详细信息

军事治理的现代化,首先应当是战略领导力的现代化。现代化的军事治理是一个系统工程,战略层面的军事治理,对于整个系统的军事治理具有决定性意义。深化国防和军队改革,对人民军队战略领导、战略指挥、战略管理体系进行了全新设计,形成的战略领导力崭新维度、体系架构和合力关系,构建起了军事治理现代化的战略领导力三维模型,为战略层面的军事治理提供了理论框架和实践逻辑。用治理的理念和方式,提升战略领导力的现代化水平,就是要通过修德治本,厚植战略领导底蕴;通过精专治能,提高战略领导专业化水平;通过纠偏治体,巩固和深化改革成果;通过抓统治融,实现战略领导力的整体涌现。

关键词：军事治理战略领导力战略领导战略指挥战略管理

“每部艺术品都是艺术家的自传”——我认识的沈伟及对《诗忆东坡》的反应与解读

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艺术评论 2024年第1期 18-40页

作者：欧建平中国艺术研究院舞蹈研究所

中国东方演艺集团一直是我们引以为傲的国家级艺术院团之一,苏东坡是诗文书画及人格魅力跨越近千年时间与万里空间的北宋文人,而沈伟则是当今世界最具影响力的华人跨界现代舞蹈家之一。因此,中国东方演艺集团特邀他回国,历时近两年,精... 详细信息

中国东方演艺集团一直是我们引以为傲的国家级艺术院团之一,苏东坡是诗文书画及人格魅力跨越近千年时间与万里空间的北宋文人,而沈伟则是当今世界最具影响力的华人跨界现代舞蹈家之一。因此,中国东方演艺集团特邀他回国,历时近两年,精心创作出这部现代舞诗剧《诗忆东坡》,可谓不二的选择。可以从“何谓‘现代舞诗剧’?”“‘东坡’何许人也?”“我认识的沈伟”“对《诗忆东坡》的现场反应”“对《诗忆东坡》的微观解读”“《诗忆东坡》带来的启示与思考”共6个部分,客观、理性地解读这部对我们审美习惯提出挑战的新作品。

关键词：中国东方演艺集团苏东坡沈伟 “三合一”知识结构《诗忆东坡》

Mode-dependent dynamic gain of all-fiber FM-EDFA under various pump manipulation

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Chinese Optics Letters 2024年第2期22卷 128-134页

作者：方翼鸿张心怡黄锡恒曾研许鸥李建平付松年秦玉文 Institute of Advanced Photonics Technology School of Information EngineeringGuangdong University of TechnologyGuangzhou 510006China Guangdong Provincial Key Laboratory of Information Photonics Technology Guangdong University of TechnologyGuangzhou 510006China Key Laboratory of Photonic Technology for Integrated Sensing and Communication Ministry of EducationGuangdong University of TechnologyGuangzhou 510006China

The dynamic gain of a few-mode erbium-doped fiber amplifier(FM-EDFA)is vital for the long-haul mode division multiplexing(MDM)transmission.Here,we investigate the mode-dependent dynamic gain of an FM-EDFA under various manipulations of the pump mode.First,we numerically calculate the gain variation with respect to the input signal power,where a modedependent saturation input power occurs under different pump modes.Even under the fixed intensity profile of the pump laser,the saturation input power of each spatial mode is different.Moreover,high-order mode pumping leads to a compression of the linear amplification region,even though it is beneficial for the mitigation of the differential modal gain(DMG)arising in all guided modes.Then,we develop an all-fiber 3-mode EDFA,where the fundamental mode of the pump laser can be efficiently converted to the LP_(11)mode using the all-fiber mode-selective coupler(MSC).In comparison with the traditional LP_(01)pumping scheme,the DMG at 1550 nm can be mitigated from 1.61 dB to 0.97 dB under the LP_(11)mode pumping,while both an average gain of 19.93 dB and a DMG of less than 1 dB can be achieved from 1530 nm to 1560 nm.However,the corresponding signal input saturation powers are reduced by 0.3 dB for the LP_(01)mode and 1.6 dB for the LP_(11)mode,respectively.Both theoretical and experimental results indicate that a trade-off occurs between the DMG mitigation and the extension of the linear amplification range when the intensity profile of pump laser is manipulated.

关键词： few-mode erbium-doped fiber amplifier differential modal gain saturation input power

ON q-WIENER INDEX OF UNICYCLIC GRAPHS

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Annals of Applied Mathematics 2018年第3期34卷 263-268页

作者： Xing Feng jianping ou Shaihua Liu Faculty of Science Jiangxi University of Science and Technology Department of Math. Wuyi UniversityJiangmen

The q-Wiener index of unicyclic graphs are determined in this work. As an example of its applications, an explicit expression of q-Wiener index of caterpillar cycles is presented.

关键词： q-Wiener index unicyclic graphs caterpillar cycles

拮抗剂方案新鲜胚胎移植和冻融胚胎移植临床结局比较

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中国临床解剖学杂志 2024年第2期42卷 181-185页

作者：孙丽李欣欣李跃葛明晓欧建平葛淑琦中山大学附属第三医院生殖医学中心广州510630 中山大学附属第三医院中医科广州510630

目的比较拮抗剂方案中行新鲜胚胎移植和冻融胚胎移植的临床结局。方法回顾性分析2575例患者行拮抗剂方案助孕的临床资料,其中鲜胚移植周期1328例,冻胚移植周期1247例。结果冻胚移植组的血清AMH值、AFC显著高于鲜胚移植组,差异有统计... 详细信息

目的比较拮抗剂方案中行新鲜胚胎移植和冻融胚胎移植的临床结局。方法回顾性分析2575例患者行拮抗剂方案助孕的临床资料,其中鲜胚移植周期1328例,冻胚移植周期1247例。结果冻胚移植组的血清AMH值、AFC显著高于鲜胚移植组,差异有统计学意义(P0.05)。冻胚移植组hCG日FSH低于鲜胚移植组,E2、P高于鲜胚移植组,差异有统计学意义(P0.05)。冻胚移植组的单胎出生体重显著高于鲜胚移植组,差异有统计学意义(P<0.05)。结论拮抗剂方案中冻胚移植亦可获得较好的临床结局,可作为一种有效的补充方案。

关键词：拮抗剂方案新鲜胚胎移植冻融胚胎移植全胚冷冻

脾门血管阻断技术在腹腔镜脾部分切除术中的应用

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中华肝胆外科杂志 2024年第4期30卷 299-301页

作者：林杰区活辉刘清波马靖陈坚平王卫东南方医科大学顺德医院(佛山市顺德区第一人民医院)肝胆胰脾外科佛山528300

回顾性分析2021年1月至2022年12月在南方医科大学顺德医院行腹腔镜脾部分切除术(LPS)的8例脾良性占位病变患者的临床资料,其中男性4例,女性4例,年龄48(28,69)岁。术中均应用脾门血管阻断技术。8例患者均顺利完成LPS,无中转开腹或行脾切... 详细信息

回顾性分析2021年1月至2022年12月在南方医科大学顺德医院行腹腔镜脾部分切除术(LPS)的8例脾良性占位病变患者的临床资料,其中男性4例,女性4例,年龄48(28,69)岁。术中均应用脾门血管阻断技术。8例患者均顺利完成LPS,无中转开腹或行脾切除术患者,手术时间162.5(141.3,180.0)min,术中出血量60(20,100)ml,术后无腹腔出血、腹腔感染、胰瘘、残留脾梗死等严重并发症发生,均顺利恢复出院。术后随访3个月,均未见病变复发,未见明显脾梗死,血小板均正常。提示脾门血管阻断技术在LPS中安全、有效。

关键词：南方医科大学脾切除术脾梗死阻断技术病变复发腹腔出血胰瘘腹腔镜

Comparison of Pregnancy outcomes of High-Quality D5- and D6-Blastocyst Transfer in Hormone-Replacement Frozen-Thawed Cycles

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International Journal of Clinical Medicine 2017年第11期8卷 565-571页

作者： Weijie Xing Liuhong Cai Li Sun jianping ou Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen University Guangzhou China

This study aimed to assess pregnancy outcomes after high-quality D5- and D6-blastocyst transfer in frozen cycles of in vitro fertilization and embryo transfer and to further evaluate whether there was a difference in blastocyst development potentials with different developmental speeds and in pregnancy outcomes. A retrospective analysis was conducted to analyze 247 frozen cycles in our center from September 2015 to July 2017, which were divided into two groups: a D5-FET group with 193 cycles of D5-blastocyst transfer, and a D6-FET group with 54 cycles of D6-blastocyst transfer. Hormone replacement method was utilized to prepare frozen-cycle endometria. Pregnancy outcomes were analyzed and compared between these two groups. The mean ages of the two groups were 31.45 ± 4.43 years and 31.98 ± 4.84 years, respectively, with no statistically significant differences (P > 0.05). The difference in the endometrial thickness during transfer was also not statistically significant. The implantation rate in the D5-FET group was 60.13%, significantly higher than that in the D6-FET group (31.58%, P P ound in the abortion rate and ectopic pregnancy rate between the two groups. The implantation, biochemical pregnancy, and clinical pregnancy rates of the blastocyst D5 were all superior to those of the blastocyst D6. In clinics, therefore, D5-blastocyst transfer could be prioritized for embryo transfer.

关键词： Frozen Cycle Hormone Replacement Therapy Blastocyst Transfer

Double-Lumen Needle Follicular Flushing System versus Single-Lumen Aspiration Needle in IVF/ICSI Patients with Poor Ovarian Response: A Meta-Analysis

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Advances in Sexual Medicine 2017年第4期7卷 167-178页

作者： Weijie Xing jianping ou Liuhong Cai Xin Tao Center for Reproductive Medicine The Third Affiliated Hospital of Sun Yat-sen University Guangzhou China

Background: The present study performed a meta-analysis to comprehensively analyze existing randomized controlled trials (RCT) involving the use of double-lumen needle in patients with poor ovarian response to explore whether double-lumen needle was good for specific patients. Methods: The PubMed, EMBASE, Cochrane Library databases and two randomized controlled trials registration centers were thoroughly searched until April 2017. The clinical outcomes of IVF/ICSI cycles were compared between two groups with double-lumen needle and single-lumen needle. Results: Four RCT studies were included in this present meta-analysis. The oocytes yield was similar in two groups (OR 0.88, 95%CI 0.66 - 1.16;I2 = 4%). The procedure time with double-lumen needle was significantly longer than that with single-lumen needle (IV = 1.98, 95%CI 0.95 - 3.00;I2 = 86%). The fertilization rate with double-lumen needle was lower than that with single-lumen needle (OR 0.66, 95%CI 0.44 - 0.97;I2 = 0%). There was no significant difference of live birth rate in two groups (OR 0.76, 95%CI 0.32 - 1.76;I2 = 41%). Conclusion: Double-lumen needle could not benefit patients with a POR in terms of the number of oocytes retrieved, oocyte recovery rate, normal fertilization rate, clinical pregnancy rate, and live birth rate, compared with single-lumen needle.

关键词： Double-Lumen Needle Single-Lumen Needle Poor Ovarian Response