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DecodeSTORM:A user-friendly ImageJ plug-in for quantitative data analysis in single-molecule localization microscopy

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Journal of Innovative Optical Health Sciences 2023年第6期16卷 77-89页

作者： Qihang Song Cheng Wu jianming huang Zhiwei Zhou Zhen-Li huang Zhengxia Wang Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical Engineering Hainan University Haikou 570228P.R.China School of Computer Science and Technology Hainan University Haikou 570228P.R.China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China

Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular level.In the past decade,several quantitative methods were developed for analyzing SMLM data;however,imaging artifacts in SMLM experiments reduce the accuracy of these methods,and these methods were seldom designed as user-friendly tools.Researchers are now trying to overcome these di±culties by developing easyto-use SMLM data analysis software for certain image analysis tasks.But,this kind of software did not pay su±cient attention to the impact of imaging artifacts on the analysis accuracy,and usually contained only one type of analysis task.Therefore,users are still facing di±culties when they want to have the combined use of different types of analysis methods according to the characteristics of their data and their own needs.In this paper,we report an ImageJ plug-in called DecodeSTORM,which not only has a simple GUI for human–computer interaction,but also combines artifact correction with several quantitative analysis methods.DecodeSTORM includes format conversion,channel registration,artifact correction(drift correction and localization¯ltering),quantitative analysis(segmentation and clustering,spatial distribution statistics and colocalization)and visualization.Importantly,these data analysis methods can be combined freely,thus improving the accuracy of quantitative analysis and allowing users to have an optimal combination of methods.We believe DecodeSTORM is a user-friendly and powerful ImageJ plug-in,which provides an easy and accurate data analysis tool for adventurous biologists who are looking for new imaging tools for studying important questions in cell biology.

关键词： Single-molecule localization microscopy artifact correction quantitative analysis ImageJ plug-in DecodeSTORM

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Effect of Heat Treatment on Microstructure and Mechanical Properties of Multiscale SiC_p Hybrid Reinforced 6061 Aluminum Matrix Composites

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Journal of Wuhan University of Technology(Materials Science) 2024年第1期39卷 174-181页

作者：吴健铭许晓静 ZHANG Xu LUO Yuntian LI Shuaidi huang Lin Institute for Advanced Manufacturing and Modern Equipment Technology Jiangsu UniversityZhenjiang 212013China

The performance of solid solution aging treatment on aluminum matrix composites prepared by powder metallurgy and reinforced with 6061 aluminum alloy powder as matrix;meanwhile, nano silicon carbide particles(nm Si Cp), submicron silicon carbide particles(1 μm Si Cp) and Ti particles were studied. The Al/Si Cp composite powder was prepared by high-energy ball milling, and then cold-pressed, sintered, hotextruded, and then heat-treated with different solution temperatures and aging times for the extruded composites. Optical microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy(EDS), X-ray diffractometer(XRD) and extrusion testing were used to analyze and test the microstructure and mechanical properties of aluminum matrix composites. The results show that after the multi-stage solid solution at 530 ℃×2 h+535 ℃×2 h+540 ℃×2 h, the particles are mainly equiaxed grains and uniformly distributed. There is no reinforcement agglomeration, and the surface is dense and the insoluble phase is basically dissolved. In the matrix, the strengthening effect is good, and the hardness and compressive strength are 179.43 HV and 680.42 MPa, respectively. Under this solution process, when the aluminum matrix composites are aged at 170 ℃ for 10 h, the hardness and compressive strength can reach their peaks and increase to 195.82 HV and 721.48 MPa, respectively.

关键词： aluminum matrix composites Si C particles multiscale hybrid enhancement heat treatment mechanical properties

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番茄褐色皱纹果病毒(ToBRFV)研究进展

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园艺学报 2024年第2期51卷 439-452页

作者：韦建明黄鑫李云洲梁燕贵州大学农学院贵阳550025 西北农林科技大学园艺学院陕西杨凌712100

番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)为帚状病毒科(Virgaviridae)烟草花叶病毒属(Tobamovirus)RNA病毒,可侵染包括番茄在内的多个科属植物,严重威胁安全生产。ToBRFV于2014年首次在以色列发现,随后蔓延至多个... 详细信息

番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)为帚状病毒科(Virgaviridae)烟草花叶病毒属(Tobamovirus)RNA病毒,可侵染包括番茄在内的多个科属植物,严重威胁安全生产。ToBRFV于2014年首次在以色列发现,随后蔓延至多个国家和地区。2021年4月中国将该病毒列为检疫性病毒。本文中对该病毒的基因组结构、寄主范围、发病症状、传播方式、检测方法、寄主抗性以及防御措施等的研究进展进行了归纳总结,以期为防控和抗ToBRFV育种提供参考和借鉴。

关键词：番茄番茄褐色皱纹果病毒抗病育种

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物象与观念:礼器对中国早期艺术的介入路径

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南京艺术学院学报（美术与设计） 2024年第1期 105-109页

作者：黄月娥谢建明南京艺术学院人文学院江苏南京210003

早期中国艺术的源流及特征之形成,可以在先秦礼器的逐步发展中找到一条清晰的主线:简单加工物——人格化力量的萌发——群体交流的实现——财富、权力、道德的社会价值表达。礼器历经了先商、商周和西周三个阶段,其价值塑造相互环流,形... 详细信息

早期中国艺术的源流及特征之形成,可以在先秦礼器的逐步发展中找到一条清晰的主线:简单加工物——人格化力量的萌发——群体交流的实现——财富、权力、道德的社会价值表达。礼器历经了先商、商周和西周三个阶段,其价值塑造相互环流,形成中国早期艺术的价值认知和特征,并过渡到秦汉古典帝国时期,形成中国庞大艺术资源的基础。

关键词：早期中国艺术礼器物象介入路径

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中国全球价值链结构性权力与对外直接投资溢出——基于联通网络方法的研究

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经济问题探索 2024年第5期 168-190页

作者：黄孝武焦骜赵鑫郑建明中南财经政法大学金融学院武汉430073 对外经济贸易大学国际商学院北京100029

在全球价值链中,相对于价值链分工地位,各国在其中的结构性权力更能直接地反映出各国在全球价值链中所掌握的主导权。本文从中国的全球价值链参与及对外直接投资活动出发,基于联通网络方法研究了中国价值链结构性权力优势对中国跨境直... 详细信息

在全球价值链中,相对于价值链分工地位,各国在其中的结构性权力更能直接地反映出各国在全球价值链中所掌握的主导权。本文从中国的全球价值链参与及对外直接投资活动出发,基于联通网络方法研究了中国价值链结构性权力优势对中国跨境直接投资溢出的影响。本文发现中国在全球价值链中的结构性权力不断增强,在国际跨境直接投资网络中的中心地位特征日益显著。而且,中国在全球价值链中的相对优势会给东道国带来更大的对外直接投资冲击溢出,这种影响对金融市场开放程度更高,与中国地理距离、制度距离更远,以及中高技术产出比例较高的国家和地区更为明显。本文研究同时将中国视作全球价值链中的主体和国际跨境直接投资母国,从全球价值链布局,以及合理调整对外直接投资结构与规模的角度,为实现“双循环”新发展格局提供了理论与经验证据。

关键词：全球价值链结构性权力跨境国际直接投资网络效应

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Tislelizumab in previously treated, locally advanced unresectable/metastatic microsatellite instability-high/mismatch repair-deficient solid tumors

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Chinese Journal of Cancer Research 2024年第3期36卷 257-276页

作者： Jian Li Ye Xu Aimin Zang Yunong Gao Quanli Gao Yanqiao Zhang Dong Wang jianming Xu Ying Yuan Haiping Jiang Jieer Ying Chunmei Shi Yanhong Deng Jing Wang Tianshu Liu Yi huang Xiaoping Qian Yueyin Pan Ying Cheng Sheng Hu Jin Wang Mengyue Shi Ke Wang Han Hu Lin Shen Department of Medical Oncology the First Affiliated Hospital Zhejiang University School of Medicine Department of Medical Oncology Cancer Hospital of the University of Chinese Academy of Sciences Department of Medical Oncology Fujian Medical University Union Hospital Department of Oncology the Sixth Affiliated Hospital Sun Yat-sen University Department of Medical Oncology Hunan Cancer Hospital Department of Medical Oncology Zhongshan HospitalFudan University Department of Immunotherapy Hubei Cancer Hospital Department of Medical Oncology the Affiliated Hospital of Nanjing University Medical School Department of Medical Oncology Anhui Provincial Hospital Department of Medical Oncology Jilin Cancer Hospital State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational Research Department of Gastrointestinal Oncology Peking University Cancer Hospital&Institute Department of Medical Oncology Hubei Cancer Hospital Clinical Development BeiGene(Shanghai)Co. Ltd. Biostatistics BeiGene(Beijing)Co. Ltd. Department of Oncology Fudan University Shanghai Cancer Center Department of Medical Oncology Affiliated Hospital of Hebei University Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Gynecology Peking University Cancer Hospital&Institute Department of Immunotherapy Henan Cancer Hospital Department of Gastrointestinal Oncology Affiliated Cancer Hospital of Harbin Medical University Department of Gastrointestinal Oncology Chongqing University Cancer Hospital Department of Gastrointestinal Oncology the Fifth Medical Center Chinese PLA General Hospital Department of Medical Oncology the Second Affiliated Hospital Zhejiang University School of Medicine

Objective: The open-label, phase Ⅱ RATIONALE-209 study evaluated tislelizumab(anti-programmed cell death protein 1 antibody) as a tissue-agnostic monotherapy for microsatellite instability-high(MSI-H)/mismatch repair-deficient(dMMR) tumors.Methods: Adults with previously treated, locally advanced unresectable or metastatic MSI-H/dMMR solid tumors were enrolled. Patients received tislelizumab 200 mg intravenously every 3 weeks. Objective response rate(ORR; primary endpoint), duration of response(DoR), and progression-free survival(PFS) were assessed by independent review committee(Response Evaluation Criteria in Solid Tumors v1.1).Results: Eighty patients were enrolled and treated; 75(93.8%) patients had measurable disease at baseline. Most had metastatic disease and received at least one prior therapy for advanced/metastatic disease(n=79; 98.8%). At primary analysis(data cutoff July 8, 2021; median follow-up 15.2 months), overall ORR [46.7%; 95% confidence interval(95% CI), 35.1-58.6; one-sided P<0.0001] and ORR across tumor-specific subgroups [colorectal(n=46):39.1%(95% CI, 25.1–54.6); gastric/gastroesophageal junction(n=9): 55.6%(95% CI, 21.2-86.3); others(n=20):60.0%(95% CI, 36.1-80.9)] were significantly greater with tislelizumab vs. a prespecified historical control ORR of 10%; five(6.7%) patients had complete responses. Median DoR, PFS, and overall survival were not reached with long-term follow-up(data cutoff December 5, 2022; median follow-up 28.9 months). Tislelizumab was well tolerated with no unexpected safety signals. Treatment-related adverse events(TRAEs) of grade ≥3 occurred in 53.8% of patients; 7.5% of patients discontinued treatment due to TRAEs.Conclusions: Tislelizumab demonstrated a significant ORR improvement in patients with previously treated,locally advanced unresectable or metastatic MSI-H/dMMR tumors and was generally well tolerated.

关键词： Biomarkers DNA mismatch repair immune checkpoint inhibitors microsatellite instability phase II clinical trials programmed cell death 1 receptor

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Salicylic acid sensitizes cervical cancer cells to radiotherapy by activating AMPK/TSC2/mTOR pathway

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Radiation Medicine and Protection 2022年第1期3卷 9-15页

作者： Shun Lu Wenjing Ye Jie Zhou Guangming Yi jianming huang Siyao Deng Minglun Li Yimin Li Yanqiong Song Jiayu Zhang Lichun Wei Guiquan Zhu Jinyi Lang Department of Radiation Oncology Sichuan Cancer Hospital&InstituteSichuan Cancer CenterSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610042China Department of Radiological Protection Radiation Oncology Key Laboratory of Sichuan ProvinceChengdu 610042China School of Medicine University of Electronic Science and Technology of ChinaChengdu 610042China The Third Hospital of Mianyang Sichuan Mental Health CenterMianyangSichuan 621054China Department of Radiation Oncology University HospitalLMUMunichGermany Department of Radiation Oncology Xijing HospitalAir Force Medical UniversityXi'anShaanxi 610599China State Key Laboratory of Oral Diseases National Clinical Research Centre for Oral DiseasesDepartment of Head and Neck OncologyWest China Hospital of StomatologySichuan UniversityChengdu 610041China

Objective:To investigate the radio-sensitizing effect of salicylic acid(SA)on human cervical cancer cells and its potential molecular mechanism.Methods:Cervical cancer cells were treated with SA and ionizing radiation.The expression ofγ-H2AX was evaluated by immunofluorescence(IF)assay.Cell cycle and apoptosis were analyzed by flow cytometry.Western blot was performed to detect the protein level of AMPK/TSC2/mTOR pathway.Results:SA inhibited basal proliferation of cervical cancer cells in a dose and time dependent manner.In addition,SA increased radiation-induced DNA damage,promoted apoptosis,triggered a redistribution of cell cycle from G2-M phase to G1-S phase of cervical cancer cells,and hence increased cell sensitivity to radiation.Moreover,SA treatment elevated the expression levels of p-AMPKα(t=3.996,P<0.05)and p-TSC2(t=5.308,P<0.05),whereas the level of p-mTOR(t=10.160,P<0.05)was significantly decreased.Conclusion:SA enhances the radiosensitivity of cervical cancer cells by targeting AMPK/TSC2/mTOR signaling pathway,and might serve as a promising therapeutic strategy to improve the efficacy of radiotherapy for cervical cancer.

关键词： Cervical cancer Salicylic acid Radiosensitivity AMPK/TSC2/mTOR pathway

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面向深部地热岩体的弹性波逆时偏移成像方法

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中国石油大学学报（自然科学版） 2024年第1期48卷 55-62页

作者：黄建平杨秀金张鑫王扬州陈亮高建明中国石油大学(华东)地球科学与技术学院山东青岛266580 山东能源集团南美有限公司山东青岛266000

地热能作为一种清洁能源正受到全世界的日益关注,因此对于深部地热岩体的高精度成像格外重要。传统的弹性波逆时偏移方法是利用耦合纵横波直接成像,从而得到耦合波场的成像结果;然而这种方法可能会出现串扰假象,导致成像结果不够清晰;... 详细信息

地热能作为一种清洁能源正受到全世界的日益关注,因此对于深部地热岩体的高精度成像格外重要。传统的弹性波逆时偏移方法是利用耦合纵横波直接成像,从而得到耦合波场的成像结果;然而这种方法可能会出现串扰假象,导致成像结果不够清晰;为了解决这个问题,采用基于解耦的弹性波方程,实现纵横波场的分离;通过利用内积成像条件,对两个典型的干热岩模型进行数值测试。结果表明,相较于耦合波场成像结果,基于解耦方程分离得到的弹性波成像剖面具有更清晰的同相轴,深部能量更加均衡,该方法能够实现对深部地热岩体的高质量成像。

关键词：深部地热岩体弹性波成像纵横波分离逆时偏移

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SiC颗粒包覆氧化铝隔绝膜的性能

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有色金属工程 2024年第3期14卷 10-14页

作者：郭睿刘建明黄凌峰王帅北矿新材科技有限公司北京102206 北京市工业部件表面强化与修复工程技术研究中心北京102206 特种涂层材料与技术北京市重点实验室北京102206

为了防止SiC颗粒与过渡金属在高温下混合应用时发生反应,使用流化床PECVD在名义粒度180μm SiC颗粒表面镀覆完整的Al_(2)O_(3)隔绝膜。通过SEM、XRD等对膜层的形态、组成和物相进行了表征,利用混合烧结法模拟SiC颗粒与过渡金属在高温下... 详细信息

为了防止SiC颗粒与过渡金属在高温下混合应用时发生反应,使用流化床PECVD在名义粒度180μm SiC颗粒表面镀覆完整的Al_(2)O_(3)隔绝膜。通过SEM、XRD等对膜层的形态、组成和物相进行了表征,利用混合烧结法模拟SiC颗粒与过渡金属在高温下混合应用的状态。当膜层厚度在4μm以上,膜层主要成分为无定形氧化铝和少量含碳杂质。在700℃/1060℃的温度和空气氛围中保温12 h,膜层发生相变,生成γ-Al_(2)O_(3)/α-Al_(2)O_(3)。经高温真空腐蚀实验验证,证实膜层能有效防止SiC与Ni等过渡金属反应,提高了SiC颗粒高温应用的性能。

关键词：流化床 PECVD 氧化铝膜隔绝膜

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共同富裕视域下浙江乡村旅游发展——以丽水松阳为例

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温州职业技术学院学报 2024年第1期24卷 15-21页

作者：周鑫蓉黄建明浙江广厦建设职业技术大学浙江东阳322100

共同富裕背景下,旅游业发展有了新机遇、新方向、新使命和新要求。浙江松阳践行“绿水青山就是金山银山”的理念,大力发展乡村旅游,为旅游业发展提供了可借鉴经验。乡村旅游产业发展是否兴旺,社区居民满意度是衡量的根本标尺。浙江乡村... 详细信息

共同富裕背景下,旅游业发展有了新机遇、新方向、新使命和新要求。浙江松阳践行“绿水青山就是金山银山”的理念,大力发展乡村旅游,为旅游业发展提供了可借鉴经验。乡村旅游产业发展是否兴旺,社区居民满意度是衡量的根本标尺。浙江乡村旅游地要构建产业融合的乡村旅游发展模式,探索乡村旅游吸引物产权制度,培育乡村旅游领军人才,深度挖掘乡村文化,做好乡村旅游新媒体营销;要把握时代赋予的重大历史机遇,积极响应共同富裕的要求,进行产业升级,扩大其经济和社会效益,提升居民满意度,促进共同富裕示范区建设。

关键词：共同富裕乡村旅游丽水松阳

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