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Flexible wearable sensors:An emerging platform for monitoring of bacterial infection in skin wounds

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Engineered Regeneration 2024年第2期5卷 186-198页

作者： Hao Meng Weicheng Zhong Kui Ma jianlong su Liqian Ma Yaying Hao Yufeng Jiang Xi Liu Xiaobing Fu Cuiping Zhang Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Department PLA General Hospital and PLA Medical CollegeBeijing 100048PR China PLA Key Laboratory of Tissue Repair and Regenerative Medicine Beijing 100048PR China Beijing Key Research Laboratory of Skin Injury Repair and RegenerationBeijing 100048PR China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048PR China College of Graduate Tianjin Medical UniversityTianjin300070PR China Department of Tissue Regeneration and Wound Repair PLA General HospitalBeijingPR China

Persistent inflammatory responses often occur when bacteria and other microorganisms frequently invade and colonize open wounds and eventually result in the formation of chronic ***,achieving real-time detection of invasive bacteria accurately and promptly is essential for efficient wound management and accelerat-ing the healing ***,flexible wearable sensors have garnered significant attention,especially those designed for monitoring real-time biophysical or biochemical signals in wound sites in a minimally invasive *** provide more precise and continuous monitoring data,making them as emerging tools for clinical *** this review,we first discuss the species and community distribution of different types of bacteria in chronic ***,we introduce currently developed techniques for detecting bacteria at wound ***-lowing that,we discuss the recent progress and unresolved issues of various flexible wearable sensors in detecting bacteria at wound *** believe that this review can provide meaningful guidance for the development of flexible wearable sensors for bacteria detection.

关键词： Flexible wearable sensors Chronic wounds Bacterial infections Real-time detection

P-MSC-derived extracellular vesicles facilitate diabetic wound healing via miR-145-5p/CDKN1A-mediated functional improvements of high glucose-induced senescent fibroblasts

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Burns & Trauma 2023年第1期11卷 325-348页

作者： jianlong su Qian Wei Kui Ma Yaxi Wang Wenzhi Hu Hao Meng Qiankun Li Yuehou Zhang Wenhua Zhang Haihong Li Xiaobing Fu Cuiping Zhang Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Division and the 4th Medical Center of Chinese PLA General Hospital 51 Fucheng RoadHaidian DistrictBeijing 100048China School of Medicine NanKai University94Weijin RoadNankai DistrictTianjin 300071China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU05151 Fucheng RoadHaidian DistrictBeijing 100048China Burn and Plastic surgery Zhongda Hospital Affiliated Southeast UniversityDingjiaqiao 87Gulou DistrictNanjing 210009China Department of Wound Repair Institute of Wound Repair and Regeneration MedicineSouthern University of Science and Technology HospitalSouthern University of Science and Technology School of Medicine6019 Xililiuxian RoadNanshan DistrictShenzhen 518055China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and Regeneration51 Fucheng RoadHaidian DistrictBeijing 100048China

Background:Persistent hyperglycaemia in diabetes causes functional abnormalities of human dermal fibroblasts(HDFs),partially leading to delayed skin wound *** vesicles(EVs)containing multiple pro-healing microRNAs(miRNAs)have been shown to exert therapeutic effects on diabetic wound *** present study aimed to observe the effects of EVs derived from placental mesenchymal stem cells(P-MSC-EVs)on diabetic wound healing and high glucose(HG)-induced senescent fibroblasts and to explore the underlying ***:P-MSC-EVs were isolated by differential ultracentrifugation and locally injected into the full-thickness skin wounds of diabetic mice,to observe the beneficial effects on wound healing in vivo by measuring wound closure rates and histological ***,a series of assays were conducted to evaluate the effects of low(2.28 x 1010 particles/ml)and high(4.56 x 1010 particles/ml)concentrations of P-MSC-EVs on the senescence,proliferation,migration,and apoptosis of HG-induced senescent HDFs in ***,miRNA microarrays and real-time quantitative PCR(RT-qPCR)were carried out to detect the differentially expressed miRNAs in HDFs after EVs *** RNA inhibitors,miRNA mimics,and small interfering RNA(siRNA)were used to evaluate the role of a candidate miRNA and its target genes in P-MSC-EV-induced improvements in the function of HG-induced senescent ***:Local injection of P-MSC-EVs into diabetic wounds accelerated wound closure and reduced scar widths,with better-organized collagen deposition and decreased p16INK4a *** vitro,P-MSC-EVs enhanced the antisenescence,proliferation,migration,and antiapoptotic abilities of HG-induced senescent fibroblasts in a dose-dependent ***-145-5p was found to be highly enriched in ***-145-5p inhibitors effectively attenuated the P-MSC-EV-induced functional improvements of senescent ***-145-5p mimics simulated the effects of P-MSC-EVs on funct

关键词： Diabetic wounds Mesenchymal stem cells Extracellular vesicles miR-145-5p CDKN1A Senescence Fibroblast Glucose

MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1

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Signal Transduction and Targeted Therapy 2023年第3期8卷 1224-1236页

作者： Qiankun Li Wenzhi Hu Qilin Huang Jie Yang Bingmin Li Kui Ma Qian Wei Yaxi Wang jianlong su Mengli sun Shengnan Cui Rungong Yang Haihong Li Xiaobing Fu Cuiping Zhang Department of Tissue Repair and Regeneration The First Medical CenterChinese PLA General HospitalBeijing 100853China Research Center for Tissue Repair and Regeneration Medical Innovation Research Department and the Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Dermatology Department The Fourth Medical CenterChinese PLA General HospitalBeijing 100048China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationBeijing 100048China School of Medicine NanKai UniversityTianjing 300071China Department of Wound Repair and Dermatologic surgery Taihe HospitalHubei University of MedicineShiyan 442000China

Unhealable diabetic wounds need to be addressed with the help of newer,more efficacious *** combined with biomaterials for sustained delivery of therapeutic agents are expected to bring new hope for chronic wound ***,the engineered exosomes modified for efficiently loading miR146a and attaching to silk fibroin patch(SFP)were demonstrated to promote diabetic wound *** fibroin binding peptide(SFBP)was screened through phage display,and SFBP-Gluc-MS2(SGM)and pac-miR146a-pac fusion protein were *** designed exosomes(SGM-Exos,miR146a-Exos,and SGM-miR146a-Exos)were isolated from the engineered placental mesenchymal stem cells(PMSCs)transduced with SGM or/and pac-miR146a-pac *** signals indicated SGM-Exo@SFP markedly increased the binding rate and the stability of ***,the loading efficiency of miR146a in SGM-miR146a-Exos was ten-fold higher than that in *** to untreated,SGM-miR146a-Exo-only treated,and SFP-only treated groups,SGM-miR146a-Exo@SFP drived wound healing associated with less inflammation,collagen deposition,and *** transcriptomics analysis suggested anti-inflammatory and regenerative effects with SGM-miR146a-Exo@SFP ***,we show efficient exosome@biomaterial-based miRNA delivery systems for regenerative medicine and tissue engineering.

关键词： miR146a wound healing

Development of the Highly Homogeneous Magnetic Field for Simulative Gyrotron by HTS

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Southwestern Institute of Physics Annual Report

Southwestern Institute of Physics Annual Report 2004年第1期 152-154页

作者： HOU Binglin ZHU Xuewu LIN jianlong su Bin

The low temperature superconducting magnet system, with the highly homogeneous magnetic field, has been applied extensively, and has had important effects on many domains. With the development of the cryogenic and superconducting techniques, it is possible that high temperature superconducting magnet with highly homogeneous magnetic field is manufactured by high temperature superconductor（HTS）. Therefore, a simulative gyrotron applied into war, with highly homogeneous magnetic field, is designed and manufactured by high temperature superconducting material.

关键词： HTS Highly homogeneous magnetic field Gyrotron

托卡马克等离子体的高精度快响应平衡控制系统的研究

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自动化学报 1999年第4期25卷 524-527页

作者：苏建龙潘垣刘保华中国科学院等离子体物理研究所

为了实现高功率波加热下等离子体的水平位移平衡控制，针对ＨＴ－６Ｍ托卡马克装置薄壁真空室的特点，成功地研制了由载流等离子体、薄壁真空室和反馈场磁体所组成的平衡控制系统．用１ＭＷ大功率ＧＴＯ直流斩波器作为反馈控制磁场电源... 详细信息

为了实现高功率波加热下等离子体的水平位移平衡控制，针对ＨＴ－６Ｍ托卡马克装置薄壁真空室的特点，成功地研制了由载流等离子体、薄壁真空室和反馈场磁体所组成的平衡控制系统．用１ＭＷ大功率ＧＴＯ直流斩波器作为反馈控制磁场电源，并采用Ｂａｎｇ－Ｂａｎｇ控制模式的技术，使该系统响应快、控制精度高．在各种实验条件下，等离子体的实际水平位置控制在±３ｍｍ以内，相应的控制精度小于１．５％．

关键词：核聚变等离子体托卡马克装置平衡控制系统

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电磁法仪器高精度GPS同步系统设计

中南大学学报（自然科学版） 2016年第12期47卷 4035-4040页

作者：于生宝何建龙苏发李刚孙长玉吉林大学仪器科学与电气工程学院吉林长春130061

为了提高电磁法勘探仪器同步系统的同步精度和抗干扰性,提出一种新的基于全球定位系统(GPS)和恒温晶振(OCXO)的同步系统方案。通过对GPS秒脉冲特性和OCXO频率特性的分析,采用卡尔曼滤波对秒脉冲中的随机噪声进行滤波处理,提高其精度。... 详细信息

为了提高电磁法勘探仪器同步系统的同步精度和抗干扰性,提出一种新的基于全球定位系统(GPS)和恒温晶振(OCXO)的同步系统方案。通过对GPS秒脉冲特性和OCXO频率特性的分析,采用卡尔曼滤波对秒脉冲中的随机噪声进行滤波处理,提高其精度。并以处理后的秒脉冲信号作为基准在线校正OCXO基本时钟,保证其输出频率的精度。采用具有电磁兼容设计的MC9S12XS128微控制器实现秒脉冲的卡尔曼滤波和OCXO在线PID校正,提高系统的抗干扰能力。研究结果表明:GPS锁定时同步脉冲精度稳定在25 ns以内。该方案提高同步系统的同步精度和抗干扰性,同时可以提高仪器野外工作的效率。

关键词：电磁法仪器 GPS同步卡尔曼滤波 OCXO校正

川东南焦石坝地区海相泥页岩含气量预测方法探讨

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石油物探 2016年第4期55卷 597-605页

作者：陈超屈大鹏王明飞苏建龙中国石油化工股份有限公司勘探分公司四川成都610041

泥页岩的含气量反映了页岩气是否高产富集,是页岩气勘探选区及评价的关键参数之一。国内外学者在单井页岩含气量的测定和预测方面进行了大量的研究,但是综合利用地球物理技术进行页岩气含气量纵向及平面预测及评价的研究较少。以四川盆... 详细信息

泥页岩的含气量反映了页岩气是否高产富集,是页岩气勘探选区及评价的关键参数之一。国内外学者在单井页岩含气量的测定和预测方面进行了大量的研究,但是综合利用地球物理技术进行页岩气含气量纵向及平面预测及评价的研究较少。以四川盆地东南缘志留系海相页岩气勘探井为基础,统计分析认为有机碳含量(CTOC)及压力系数是影响页岩含气量的关键指标,建立了页岩含气量多元计算模型。首先基于岩性密度与总有机碳预测模型,采用高精度叠前密度反演技术,实现CTOC的地震定量预测;其次通过叠前速度反演技术,利用改进的Fillippone地层压力系数计算公式进行压力系数的预测;最后综合CTOC及压力系数进行页岩总含气量的预测。该方法较CTOC单参数模型预测精度明显提高,且在构造复杂、压力系数差异明显的地区具有很好的推广应用前景。

关键词：含气量 CTOC 叠前反演 Fillippone地层压力系数计算公式速度反演

人蜕膜间充质干细胞来源外泌体对高糖老化人真皮成纤维细胞功能的影响及其可能机制

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中华烧伤与创面修复杂志 2022年第2期38卷 170-183页

作者：苏建隆马奎张翠萍付小兵南开大学医学院天津300071 解放军总医院医学创新研究部创伤修复与组织再生研究中心北京100853 中国医学科学院严重创伤救治和组织修复与再生医学创新单元2019RU051 北京100048

目的建立人真皮成纤维细胞(HDF)高糖老化模型,探讨人蜕膜间充质干细胞(dMSC)来源外泌体对高糖老化HDF增殖、迁移、凋亡的影响及其可能机制。方法采用实验研究方法。收集2021年1—3月解放军总医院第四医学中心收治的4例男性包茎患者(18~2... 详细信息

目的建立人真皮成纤维细胞(HDF)高糖老化模型,探讨人蜕膜间充质干细胞(dMSC)来源外泌体对高糖老化HDF增殖、迁移、凋亡的影响及其可能机制。方法采用实验研究方法。收集2021年1—3月解放军总医院第四医学中心收治的4例男性包茎患者(18~22岁)环切术后废弃包皮组织,分离培养获取原代HDF。取第6代HDF,按照随机数字表法分为低糖组和高糖组,分别采用低糖完全培养基和高糖完全培养基进行每72小时换液、不传代培养,10 d后取细胞,于接种后24 h,采用β-半乳糖苷酶试剂盒检测细胞衰老情况;于接种后48 h,采用蛋白质印迹法检测细胞衰老相关蛋白p16、p53表达情况;于接种后24、48、72 h,采用细胞计数试剂盒8(CCK-8)法检测细胞增殖情况;于接种后48 h,采用脱氧尿嘧啶核苷(EdU)染色法检测细胞增殖情况,采用流式细胞术检测细胞周期及凋亡情况;于接种后24 h,采用Transwell实验测定细胞迁移能力。取人dMSC培养48~72 h,采用差速高速离心法获取其外泌体,采用透射电子显微镜观察dMSC外泌体形态,采用纳米颗粒追踪分析法检测dMSC外泌体的粒径分布,采用蛋白质印迹法检测dMSC外泌体标志蛋白CD9、肿瘤易感基因101(TSG101)的表达。取dMSC外泌体及前述高糖完全培养基诱导老化的HDF共孵育24 h,采用PKH67试剂盒检测细胞摄取外泌体的情况。取前述高糖完全培养基诱导老化的HDF,同前分为单纯高糖组、高糖+低浓度外泌体组、高糖+高浓度外泌体组,分别于高糖完全培养基中加入等体积的磷酸盐缓冲液、终质量浓度为50μg/mL dMSC外泌体、终质量浓度为100μg/mL dMSC外泌体进行常规细胞培养。分组后同前于对应时间点采用CCK-8法和EdU染色法、流式细胞术、Transwell实验分别检测细胞增殖、细胞周期和凋亡及细胞迁移情况。根据前述结果,另取经高糖完全培养基诱导老化的HDF,分为单纯高糖组、高糖+高浓度外泌体组并同前处理。分组培养48 h,采用实时荧光定量反转录PCR法检测单纯高糖组和高糖+高浓度外泌体组细胞衰老相关的微小RNA-145-5p(miR-145-5p)、miR-498、miR-503-5p及其靶基因钙/钙调素依赖性蛋白激酶1D(CAMK1D)、人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(PTEN基因)和细胞周期蛋白D1的mRNA表达情况。对数据行析因设计方差分析、单因素方差分析、LSD-t检验和独立样本t检验。结果接种后24 h,高糖组HDFβ-半乳糖苷酶阳性染色率为(38.4±4.2)%,明显高于低糖组的(16.5±2.2)%(t=4.65,P<0.01)。接种后48 h,高糖组HDF的衰老相关蛋白p16和p53的表达量均明显高于低糖组(t值分别为11.85、3.02,P<0.05或P<0.01)。接种后24、48、72 h,高糖组HDF的增殖活性均明显低于低糖组(t值分别为4.13、9.90、15.12,P<0.01)。接种后48 h,高糖组HDF的EdU阳性染色率明显低于低糖组(t=3.83,P<0.05)。接种后48 h,高糖组HDF周期的G2/M+S亚群在3个亚群(G0/G1、S和G2/M)的占比明显低于低糖组(t=8.74,P<0.01)。接种后24 h,高糖组HDF穿过Transwell滤膜到达下室的细胞数量为(37±6)个,明显少于低糖组的(74±7)个(t=8.42,P<0.01)。接种后48 h,高糖组HDF凋亡率明显高于低糖组(t=8.48,P<0.01)。dMSC外泌体为边缘清晰、大小分布均匀的杯状或者圆形囊泡,粒径基本处于80~200 nm。dMSC外泌体标志性蛋白CD9、TSG101表达均呈阳性。共孵育24 h,外泌体被HDF摄入胞内,主要分布于细胞核周围。分组培养24、48、72 h,高糖+低浓度外泌体组和高糖+高浓度外泌体组HDF增殖活性均明显高于单纯高糖组(t值分别为6.36、6.10、7.76,8.92、12.17、10.74,P<0.01),高糖+高浓度外泌体组HDF增殖活性均明显高于高糖+低浓度外泌体组(t值分别为7.92、4.82、4.72,P<0.01)。分组培养48 h,与单纯高糖组比较,高糖+低浓度外泌体组和高糖+高浓度外泌体组HDF EdU阳性染色率均明显升高(t值分别为5.32、9.88,P<0.01);与高糖+低浓度外泌体组比较,高糖+高浓度外泌体组HDF EdU阳性染色率显著升高(t=5.27,P<0.01)。分组培养48 h,与单纯高糖组比较,高糖+低浓度外泌体组和高糖+高浓度外泌体组HDF中的G0/G1期亚群占比均显著降低(t值分别为3.81、4.31,P<0.05),G2/M+S亚群占比均明显升高(t值分别为3.

关键词：间质干细胞外泌体细胞衰老葡萄糖溶液,高渗成纤维细胞

基于卡尔曼滤波的GM(1,1)模型在三维激光扫描边坡沉降变形分析中的应用

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地理信息世界 2018年第4期25卷 69-74,85页

作者：党星海苏建龙兰州理工大学甘肃兰州730050 兰州理工大学建筑勘察设计研究院甘肃兰州730050

利用三维地面激光扫描技术进行边坡变形监测时,由于其监测的数据容易受树木、行人、行车、电线杆等外界因素的干扰,所得到的结果是一组含噪声较多的边坡沉降时间序列。通过卡尔曼滤波对初始监测数据进行去噪处理,可以平滑掉曲线上波动... 详细信息

利用三维地面激光扫描技术进行边坡变形监测时,由于其监测的数据容易受树木、行人、行车、电线杆等外界因素的干扰,所得到的结果是一组含噪声较多的边坡沉降时间序列。通过卡尔曼滤波对初始监测数据进行去噪处理,可以平滑掉曲线上波动较大的尖点,得到更合理的沉降曲线,有效提高模型的预测精度。基于以上理论,本文采用三维激光扫描仪对西北某湿陷性黄土边坡进行扫描,将得到的点云数据采用重心法提取特征点的高程坐标,再对数据采用卡尔曼滤波算法进行滤波去噪处理,通过建立基于卡尔曼滤波算法的新预测模型来预测其沉降变形。通过工程实例表明:建立的新模型的拟合和预测精度要优于传统灰色预测模型,具有较好的借鉴意义,为以后三维激光扫描技术在边坡变形监测中的应用提供指导。

关键词：边坡变形监测三维激光扫描技术卡尔曼滤波点云噪声灰色预测模型