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Investigation of novel zir-conium based perovskite-type mixed conducting membranes for oxygen separation

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Chinese Science Bulletin 2001年第6期46卷 473-477页

作者： jianhua tong Weishen Yang Zongping Shao Guoxing Xiong Liwu Lin State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian China

A series of BaZr0.2Co0.8-χFeχO3-σ materials for oxygen separation were synthesized through a citric and EDTA acid combined complexing method, and their crystal structures, oxygen permeabilities, sintering and sealing abilities were investigated. The results showed that the cubic perovskite structure was formed for materials in the composition range investigated. Oxygen permeation flux and stability, as well as sintering and sealing abilities of the synthesized materials were increased or improved apparently due to the introduction of zirconium. For example, the oxygen permeation flux reached 0.8 mL/min · cm2 when χ = 0.2 or 0.3 at 950℃, and a stable time-related oxygen permeation flux was found for the BaZr0.2Co0.3Fe0.5O3-σ membrane at 800℃.

关键词： perovskite mixed conductor oxygen permeation mem- brane zirconium.

Reduced grain chalkiness and its possible physiological mechanism in transgenic rice overexpressing L-GalLDH

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The Crop Journal 2015年第2期3卷 125-134页

作者： Le Yu Yonghai Liu jianhua tong Junhui Ding Ruozhong Wang Changlian Peng Langtao Xiao College of Life Sciences Zhaoqing University Hunan Provincial Key Laboratory of Phytohormones and Growth Development Hunan Agricultural University College of Life Sciences South China Normal University

Chalkiness is one of the key factors determining rice quality and price. Ascorbic acid(Asc) is a major plant antioxidant that performs many functions in plants. L-Galactono-1,4-lactone dehydrogenase(L-Gal LDH, EC1.***.2.3) is an enzyme that catalyzes the final step of Asc biosynthesis in plants. Here we show that the L-Gal LDH-overexpressing transgenic rice, GO-2,which has constitutively higher leaf Asc content than wild-type(WT) plants, exhibits significantly reduced grain chalkiness. Higher foliar ascorbate/dehydroascorbate(Asc/DHA)ratios at 40, 60, 80, and 100 days of plant age were observed in GO-2. Further investigation showed that the enhanced level of Asc resulted in a significantly higher ribulose-1,5-bisphosphate(Ru BP) carboxylase/oxygenase(Rubisco) protein level in GO-2 at 80 days. In addition, levels of abscisic acid(ABA) and jasmonic acid(JA) were lower in GO-2 at 60, 80, and100 days. The results we present here indicate that the enhanced level of Asc is likely responsible for changing redox homeostasis in key developmental stages associated with grain filling and alters grain chalkiness in the L-Gal LDH-overexpressing transgenic by maintaining photosynthetic function and affecting phytohormones associated with grain filling.

关键词： Grain chalkiness Ascorbic acid Abscisic acid Jasmonic acid Rice

Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry

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Chinese Science Bulletin 2010年第21期55卷 2231-2235页

作者： LIU Xia YANG YanLi LIN WanHuang tong jianhua HUANG ZhiGang XIAO LangTao Hunan Provincial Key Laboratory of Phytohormones and Growth Development Hunan Agricultural University Changsha 410128 China

An efficient method for the simultaneous extraction and quantification of both jasmonic acid(JA)and methyl jasmonate(MeJA) from a single plant sample was *** tissues were firstly extracted by using 100%cold methanol,then the Sep-pak C18 solid-phase extraction(SPE)cartridges were adopted for the purification of sample extract,and finally JA and MeJA were determined by the liquid chromatography tandem mass spectrometry(LC-MS-MS)*** was found that the accuracy was improved by using the extra standard for the estimation and correction of JA and MeJA losses in the extraction and purification *** detection limits for JA and MeJA were 0.03 and 0.075 ng mL-1,respectively,and the average recovery rate of JA and MeJA was 92.48%and 94.30%.The method was found to be reproducible and selective,yielding well isolated and easily detectable peaks for both JA and MeJA and simplifying the time-consuming extraction and ***-performance liquid chromatography(HPLC)system was employed as the control detection method for the LC-MS-MS *** two systems were compared in their specificity and accuracy.

关键词：高效液相色谱法茉莉酸甲酯串联质谱法植物样品测定同时提取 HPLC 提取纯化

ELECTRIC FIELD MICROSENSOR BASED ON THE STRUCTURE OF PIEZOELECTRIC INTERDIGITATED CANTILEVER BEAMS

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Journal of Electronics(China) 2014年第6期31卷 497-504页

作者： Feng Ke tong jianhua Wang Yu Fang Dongming Xia Shanhong State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences

This paper presents the design, fabrication, and preliminary experimental result of an electric field microsensor based on the structure of piezoelectric interdigitated cantilevers with staggered vertical vibration mode. The working principle of this electric field microsensor is demonstrated, and the induced charges and structural parameters of this microsensor are simulated by the finite element method. The electric field microsensor was fabricated by Micro-Electro Mechanical Systems(MEMS) technique. Each cantilever is a multilayer compound structure(Al/Si3N4/ Pt/PZT/Pt/ Ti/SiO 2/Si), and Piezoelectric, PieZ oelectric ceramic Transducer(PZT)(PbZ rxTi(1–x)O3) layer, prepared by sol-gel method, is used as the piezoelectric material to drive the cantilevers vibrating. This electric field microsensor was tested under the DC electric field with the field intensity from 0 to 5×104 V/m. The output voltage signal of the electric field microsensor has a good linear relationship to the intensity of applied electric field. The performance could be improved with the optimized design of structure, and reformative fabrication processes of PZT material.

关键词： Micro-ElectroM echanical Systems(MEMS) Electric field microsensor Piezoelectric PieZ oelectric ceramic Transducer(PZT) driven

MESOPOROUS TIO_2 NANO-SPHERES: ELECTROSPRAY COMBINED SOL-GEL FABRICATION AND APPLICATION TO ORGANIC PHOSPHORUS DEGRADATION

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Journal of Electronics(China) 2013年第3期30卷 313-317页

作者： Dong Tian tong jianhua Bian Chao Zhao Ling He Xiuli Xia Shanhong State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences

In this work, electrospray technique combined sol-gel method was used to prepare porous TiO2 film. X-ray Photoelectron Spectroscopy (XPS), X-Ray Diffraction (XRD), and Scanning Electron Microscope (SEM) analyses were conducted to examine the chemical composition, phase structure, and surface morphology of the sprayed TiO2 film. After calcined at 450℃ in air atmosphere for 2 h, mesoporous TiO2 nano-spheres clusters were formed on the surface of silicon wafer and the average size of nano-spheres was 250 nm. Ti presented as Ti 4+ oxidation state in TiO2 film, and the TiO2 film exhibited the anatase phase. The sprayed porous TiO2 films were employed as photocatalyst to degrade organic phosphorus in water samples. Compared with the TiO2 film prepared by Sol-Gel spin-coating method, the porous TiO2 film deposited by electrospray combined sol-gel method showed higher photocatalytic activity.

关键词： Sol-gel Electrospray Mesoporous TiO2 nano-spheres Organic phosphorus degradation

The alteration in the architecture of a T-DNA insertion rice mutant osmtd1 is caused by up-regulation of Micro RNA156f

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Journal of Integrative Plant Biology 2015年第10期57卷 819-829页

作者： Qing Liu Gezhi Shen Keqin Peng Zhigang Huang jianhua tong Mohammed Humayun Kabir Jianhui Wang Jingzhe Zhang Genji Qin Langtao Xiao Hunan Provincial Key Laboratory of Phytohormones and Growth Development Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization Hunan Agricultural University Crop Institute Shanghai Academy of Agricultural Sciences Horticulture Institute Sichuan Academy of Agricultural Sciences State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University

Plant architecture is an important factor for crop production. Some members of microRNA156 (miR156) and their target genes SQUAMOSA Promoter-Binding Protein-Like (SPL) were identified to play essential roles in the establishment of plant architecture. However, the roles and regulation of miR156 is not well understood yet. Here, we identified a T-DNA insertion mutant Osmtd1 (Oryza sativa multi-tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T-DNA insertion in Osmtd1. Further analysis revealed that the T-DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and OsmiR156f was significantly upregulated in Osmtd1. Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of OsmiR156f in wild-type rice phenocopied Osmtd1. We showed that the expression of OsSPL3, OsSPL12, and OsSPL14 were significantly downregulated in Osmtd1 or OsmiR156f overexpressed lines, indicating that OsSPL3, OsSPL12, and OsSPL14 were possibly direct target genes of OsmiR156f. Our results suggested that OsmiR156f controlled plant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein.

关键词： Oryza sativa OsmiR156f plant architecture protease inhibitor T-DNA insertion

Endovascular stent-induced alterations in host artery mechanical environments and their roles in stent restenosis and late thrombosis

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Regenerative Biomaterials 2018年第3期5卷 177-187页

作者： Jinxuan Wang Xuepu Jin Yuhua Huang Xiaolin Ran Desha Luo Dongchuan Yang Dongyu Jia Kang Zhang jianhua tong Xiaoyan Deng Guixue Wang Key Laboratory of Biorheological Science and Technology Ministry of EducationState and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqingChina Institute for Biomedical Engineering&Nano Science Shanghai East HospitalTongji University School of MedicineShanghaiChina Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education School of Biological Science and Medical EngineeringBeihang UniversityBeijingChina

Cardiovascular stent restenosis remains a major challenge in interventional treatment of cardiovascular occlusive *** the changes in arterial mechanical environment due to stent implantation are the main causes of the initiation of restenosis and thrombosis,the mechanisms that cause this initiation are still not fully *** this article,we reviewed the studies on the issue of stent-induced alterations in arterial mechanical environment and discussed their roles in stent restenosis and late thrombosis from three aspects:(i)the interaction of the stent with host blood vessel,involve the response of vascular wall,the mechanism of mechanical signal transmission,the process of re-endothelialization and late thrombosis;(ii)the changes of hemodynamics in the lumen of the vascular segment and(iii)the changes of mechanical microenvironment within the vascular segment wall due to stent *** review has summarized and analyzed current work in order to better solve the two main problems after stent implantation,namely in stent restenosis and late thrombosis,meanwhile propose the deficiencies of current work for future reference.

关键词： stents restenosis hemodynamics local mechanical environment

Executive function during transient ischemic attacks

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Neural Regeneration Research 2007年第3期2卷 142-145页

作者： jianhua Zhao tong Li Yanxia Liu Ping Zhang Haiqing Yan Department of Neurology the First Affiliated Hospital of Xinxiang Medical College Xinxiang 453100 Henan Province China

BACKGROUND： Recent researches demonstrate that damage of executive function is an early manifestation of vascular cognitive disorder. OBJECTIVE： To investigate the executive functions of patients with transient ischemic attack （TIA）. DESIGN： Case control. SETTING： Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College. PARTICIPANTS： A total of 83 TIA patients （46 males and 37 females, aged 32 - 74 years） were selected from Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College from July 2005 to December 2006. The diagnosis of TIA was established by the criteria of cerebrovascular diseases made by the Fourth National Cerebrovascular Disease Meeting. In these cases, forty-five patients （54%） were involved in internal carotid artery, and thirty-eight patients （46%） were involved in vertebral basilar system. Fifty healthy adults （Control group： 28 males and 22 females, aged 32 - 74 years） were chosen from retiree in community and family of patients. All of them were right-handedness, without cerebrovascular disease, cerebral trauma, inborn oligophrenia, and heating and visual disorders; also they had no anxiety and depression nearly one week. In addition, all the subjects cooperated with examination. METHODS： After TIA diagnosis, all patients received neuropsychological examination, including attention and inhibition, working memory, flexibility, planning and diversion, based on Stroop tests （C and CW） and the Wisconsin card sorting tests （WCST）. ① WCST test： The edition revised by Nelson was used. We would take the times of sorting, incorrect response, persistent incorrect response, and randomly incorrect response as the index of evaluation.② Stroop tests： The edition revised by Trenarry was used and this test had two types： form color and form color-word. Their incorrect response and response time were recorded for data analysis in the end. MAIN OUTCOME MEASURES： Results of WCST test and Stroop tests. RESULTS： A

关键词： ischemic attack, transient neuropsychological tests case control studies

Nanomaterials-mediated lysosomal regulation:a robust protein-clearance approach for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 424-439页

作者： Mengqi Hao Jianjian Chu Tinglin Zhang tong Yin Yuankai Gu Wendanqi Liang Wenbo Ji jianhua Zhuang Yan Liu Jie Gao You Yin Department of Neurology Second Affiliated Hospital of Naval Medical University(Shanghai Changzheng Hospital)ShanghaiChina School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghaiChina Changhai Clinical Research Unit Shanghai Changhai HospitalNaval Medical UniversityShanghaiChina Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurology Shanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina

Alzheimer’s disease is a debilitating,progressive neurodegenerative disorder characterized by the progressive accumulation of abnormal proteins,including amyloid plaques and intracellular tau tangles,primarily within the ***,crucial intracellular organelles responsible for protein degradation,play a key role in maintaining cellular *** studies have suggested a link between the dysregulation of the lysosomal system and pathogenesis of neurodegenerative diseases,including Alzheimer’s *** the normal physiological function of lysosomes hold the potential to reduce the pathological burden and improve the symptoms of Alzheimer’s ***,the efficacy of drugs in treating Alzheimer’s disease is limited,with major challenges in drug delivery efficiency and ***,nanomaterials have gained widespread use in Alzheimer’s disease drug research owing to their favorable physical and chemical *** review aims to provide a comprehensive overview of recent advances in using nanomaterials(polymeric nanomaterials,nanoemulsions,and carbon-based nanomaterials)to enhance lysosomal function in treating Alzheimer’s *** review also explores new concepts and potential therapeutic strategies for Alzheimer’s disease through the integration of nanomaterials and modulation of lysosomal *** conclusion,this review emphasizes the potential of nanomaterials in modulating lysosomal function to improve the pathological features of Alzheimer’s *** application of nanotechnology to the development of Alzheimer’s disease drugs brings new ideas and approaches for future treatment of this disease.

关键词： Alzheimer’s disease autophagy dysfunction lysosomal acidification lysosomal system nanomaterials neurodegenerative diseases