检索结果-南通市图书馆

Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats

Neural Regeneration Research 2006年第7期1卷 642-644页

作者： Su Liu1, Guangyu Shen1, Guangming Lü2, Xiaosong Gu3 1Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, Nantong 226001, jiangsu province, China 2Department of Human Anatomy, Nantong University, Nantong 226001, jiangsu province, China 3jiangsu Key Laboratory of Nerve Regeneration, Nantong 226001, jiangsu province, China Department of Rehabilitation Medicine Hospital of Nantong University Nantong 226001 Jiangsu Province China Department of Human Anatomy Nantong University Nantong 226001 Jiangsu Province China jiangsu Key Laboratory of Nerve Regeneration Nantong 226001 Jiangsu Province China

BACKGROUND: There are many methods for myelin staining, mordant, or oil-soluble dye or the special reaction of osmic acid with lipoid is used according to different principles. The commonly used methods are classic Well staining, classic lithium carbonate-haematine staining, fast green staining, silver staining, etc. Luxol Fast Blue can brightly stain myelin sheath, and has certain specificity. The background can be very clean if there is proper differentiation, whereas Luxol Fast Blue is cheap and convenient to operate, thus it is an ideal staining reagent for routine myelin sheath. OBJECTIVE: To show the corticospinal tract of normal adult rats with Luxol Fast Blue staining method. DESIGN: A repetitive measurement design. SETTINGS: Institute of Nuerobiology, Nantong University; Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University. MATERIALS: Six healthy adult male SD rats of clean degree, weighing averagely 300 g, were provided by the experimental animal center of Nantong University. 1 g/L Luxol Fast Blue solution was provided by Sigma Company; Leica CM1900 cryostat microtome by Leica Company; Leica DMR microscope by Leica Company. METHODS: The experiment was carried out in the Staff Room of Human Anatomy, Nantong University in May 2005. The rats were given intraperitoneal injection of combined anesthetic (2 mL/kg), then the chest was open for perfusing saline and phosphate buffer containing formamint via heart. Brain and spinal cord were removed after 1 hour then fixed, then changed to phosphate buffer (pH 7.4) containing 300 g/L saccharu at 4 ℃, and stayed overnight, tissue blocks at pyramid, decussation of pyramid and cervical, thoracic, lumbar and sacral segments of spinal cord were removed to prepare continuous horizontal frozen sections (30 μm) after sedimentation, the sections were dried at room temperature. The corticospinal tract of normal adult rats were shown with Luxol Fast Blue staining method, and observed under Leica DMR

关键词： Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

鲍卫忠工作方法的时代价值

引用

人民司法 2024年第4期 21-24页

作者： Party Group of Lincang Intermediate People's Court of Yunnan province 云南省临沧市中级人民法院

2023年12月18日,中共中央宣传部追授鲍卫忠同志“时代楷模”称号。他长期扎根祖国西南边睡,对党忠诚、公正司法,为民族地区繁荣稳定贡献法治力量;他始终珍视民族团结,深入村寨普法释法,依法保护各族群众合法权益,被当地群众亲切地称为... 详细信息

2023年12月18日,中共中央宣传部追授鲍卫忠同志“时代楷模”称号。他长期扎根祖国西南边睡,对党忠诚、公正司法,为民族地区繁荣稳定贡献法治力量;他始终珍视民族团结,深入村寨普法释法,依法保护各族群众合法权益,被当地群众亲切地称为佤山法治“老黄牛”;他严于律已、清正廉洁,彰显了新时代人民法官的政治本色。在24年执法办案和服务群众的司法实践中,鲍卫忠同志积累了丰富的工作经验和方法。鲍卫忠同志的工作方法,集中体现了习近平法治思想人民至上的理论品格与深刻的现实关怀,具有丰富的时代价值。

关键词：习近平法治思想公正司法理论品格中共中央宣传部西南边睡严于律已执法办案现实关怀

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nd, Sr and O Isotopic Study on Spilite-Keratophyre in Xiqiu,,Zhejiang province, China

引用

Acta Geologica Sinica(English Edition) 1992年第2期66卷 165-176页

作者： Shen Weizhou Zhang Bangtong Ling Hongfei Lai Mingyian Yang Jiedong Tao Xiancong Department of Earth Sciences, Nanjing University, Nanjing, jiangsu Modern Analysis Centre, Nanjing University, Nanjing, jiangsu Department of Earth Sciences Nanjing University Nanjing Jiangsu China

Nd, Sr and O isotopic study on the spilite-keratophyre sequence in Xiqiu shows that its ∈_(Nd) values are inthe range of 4.02-5.26, and its ∈_(Sr) values, +1.4-2.6. According to the points of these data in the ∈_(Nd)-T,∈_(Sr)-T and ∈_(Nd)-∈_(Sr) diagrams, the spilite-keratophyre is interpreted as being slightly contaminated by crustalmaterials. Its δ^(18)O values are 3.9-5.0‰. The depletion of ^(18)O in the rocks resulted from the influence ofseawater hydrothermal alteration during or soon after the rock formation. Based on the isotopic characteristicsand available geochemical data, it is believed that the spilite-keratophyre was formed in the well-developedisland-arc environment during the Late Proterozoic subduction of the palaeo-Pacific plate beneath thesoutheastern margin of the Yangtze massif.

关键词： Sr and O Isotopic Study on Spilite-Keratophyre in Xiqiu China Zhejiang province Nd

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analysis of the co-seismic responses of the fluid well pattern system in jiangsu province to the Wenchuan and Tohoku earthquakes

引用

Earthquake Science 2012年第3期25卷 263-274页

作者： Jiangrong Zheng1, Haolin Jiang1 and Zhengran He2 1 Earthquake Administration of jiangsu province, Nanjing 210014, China 2 School of Information Science and Engineering, Southeast University, Nanjing 210049, China 1. Earthquake Administration of jiangsu province Nanjing 210014 China2. School of Information Science and Engineering Southeast University Nanjing 210049 China

This thesis discusses the earthquake reflecting ability of the observation well pattern system of jiangsu province, China, which has been digitally renovated, and probes into the cause of the major differences in the earthquake reflecting abilities of well holes at different measurement points. This is achieved through the analysis of the co-seismic responses to the Wenchuan (2008; Ms8.0, China) and Tohoku (2011; Ms9.0, Japan) earthquakes. We found that the co-seismic response of water level from regional well holes in jiangsu province was stronger than that of water temperature. The water-level co-seismic response follows a consistent law and is closely related to the earthquake magnitude. The co-seismic response of water temperature strongly varied among well points, and was more often manifested as a slow restorative change. The co-seismic responses also varied based on tectonic elements. The response in central and northern jiangsu was weaker than that of southern jiangsu, possibly due to the thicker loess cover layer in central jiangsu which makes it less effective at capturing the micro-changes of stress-strain states relative to the hilly land in the south. The more complicated geological structure in southern jiangsu makes it contribute to greater changes in the state of underground water after a minor disturbance.

关键词： fluid well pattern system 2008 Ms8.0 Wenchuan earthquake 2011 MS9.0 Tohoku earthquake features of co-seismic responses

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mitochondrial uncoupling protein 2 expression in colon cancer and its clinical significance

引用

World Journal of Gastroenterology 2010年第45期16卷 5773-5778页

作者： Xiao-Yi Kuai, Ze-Yu Ji, Hong-Jie Zhang,Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, jiangsu province, China Department of Gastroenterology First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road Nanjing 210029 Jiangsu Province China. hjzhang06@***

AIM: To detect the expression of mitochondrial uncoupling protein 2 (UCP2) in colon cancer and analyze the relation between UCP2 expression and clinical pathological features of colon ***: Fifteen colon tissue samples and 15 its adjacent tissue samples were obtained from colon cancer patients during surgical interventions. UCP2 expression was detected with immunohistochemical method in 10 normal controls, 10 hyperplastic polyp patients, 20 tubular adenoma patients and 78 colon cancer patients. Patients with rectal cancer were excluded. Quantitative reverse transcription polymerase chain reaction and Western blotting were used to detect UCP2 expressions in colon cancer tissue samples and its adjacent tissue samples. Relation between UCP2 expression and clinical pathological features of colon cancer was also analyzed. RESULTS: The UCP2 mRNA expression level was fourfold higher in colon cancer tissue samples than in its adjacent tissue samples. The UCP2 protein expression level was three-fold higher in colon cancer tissue samples than in its adjacent normal tissue samples. The UCP2 was mainly expressed in cytoplasm. The UCP2 was not expressed in normal colon mucosa. Strong positive staining for UCP2 with a diffuse distribution pattern was identified throughout the mucosa in colon cancer tissue samples with a positive expression rate of 85.9%. The UCP2 expression level was higher in colon cancer tissue samples at clinical stages Ⅲ and Ⅳ than in those at stageⅠ+ Ⅱ. Univariate analysis showed that the high UCP2 expression level was significantly correlated to colon cancer metastasis (hazard ratio = 4.321, confidence interval = 0.035-0.682, P = 0.046). CONCLUSION: UCP2 is highly expressed in human colon cancer tissue and may be involved in colon cancer metastasis.

关键词： Mitochondrial uncoupling protein 2 Colon cancer Uncoupling protein 2 Clinicopathologic characteristics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

公司僵局预防机制研究——以公司治理中双层股权结构的构建为路径

引用

人民司法 2024年第13期 81-86页

作者：江苏省苏州市吴江区人民法院课题组吴岚不详

公司僵局的形成对公司利益及股东权益均会造成严重损害。股东股权转让、异议股东回购、法院强制股权转让、司法解散等事后救济措施固然能起到化解公司僵局的作用,但事前预防机制的有效设立,能够最大程度降低僵局对公司运营的影响。在发... 详细信息

公司僵局的形成对公司利益及股东权益均会造成严重损害。股东股权转让、异议股东回购、法院强制股权转让、司法解散等事后救济措施固然能起到化解公司僵局的作用,但事前预防机制的有效设立,能够最大程度降低僵局对公司运营的影响。在发挥公司章程自治作用、明确股东的责任义务以及强化企业信息披露的理念引导下,公司采用双层股权结构治理可以破除公司僵局。采取双层股权结构的公司可以通过明确表决权的比例、幅度和适用范围,在章程中约定合理的固定期限型日落条款以及持续性的信息披露,有效整合控制偏好型股东和利益偏好型股东的异化需求,以灵活配置的方式满足不同股东的竞争利益,优化公司治理结构,促成决议的有效形成,避免公司治理停滞。

关键词：事前预防公司治理结构预防机制责任义务公司僵局信息披露股东权益严重损害

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clinical　Use　of　Medicinal　Cake　Moxibustion　Apparatus　and　Acupuncture　and　Moxibustion　Clothes　and　Quilt

引用

中国针灸 1995年第S2期15卷 381-382页

作者： Zhuang Hanguang, Zhao Yan(Baipu Central Hospital, Rugao City, jiangsu province.226511)(Nanyang Institute of Science and Technology,Henan province)

ＣｌｉｎｉｃａｌＵｓｅｏｆＭｅｄｉｃｉｎａｌＣａｋｅＭｏｘｉｂｕｓｔｉｏｎＡｐｐａｒａｔｕｓａｎｄＡｃｕｐｕｎｃｔｕｒｅａｎｄＭｏｘｉｂｕｓｔｉｏｎＣｌｏｔｈｅｓａｎｄＱｕｉｌｔ￥ＺｈｕａｎｇＨａｎｇｕａｎｇ，Ｚｈａ... 详细信息

ＣｌｉｎｉｃａｌＵｓｅｏｆＭｅｄｉｃｉｎａｌＣａｋｅＭｏｘｉｂｕｓｔｉｏｎＡｐｐａｒａｔｕｓａｎｄＡｃｕｐｕｎｃｔｕｒｅａｎｄＭｏｘｉｂｕｓｔｉｏｎＣｌｏｔｈｅｓａｎｄＱｕｉｌｔ￥ＺｈｕａｎｇＨａｎｇｕａｎｇ，ＺｈａｏＹａｎ（ＢａｉｐｕＣｅｎｔｒａｌ...

关键词： Cake

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Susceptibility to hepatocellular carcinoma associated with null genotypes of GSTM] and GSTT1

引用

World Journal of Gastroenterology 2000年第2期6卷 228-230页

作者： Jian Chao Bian Fu Min Shen Li Shen Tian Ru Wang Xiao Hong Wang Gong Chao Chen Jin Bing Wang Department of Epidemiology,Shanghai Medical University,Shanghai 200032,China Haimen Municipal Anti-Epidemic and Health Station,Haimen 226201,jiangsu province,China Qidong Institute for Liver Cancer,Qidong 226200,jiangsu province,China 上海医科大学上海江苏海门市卫生防疫站江苏海门江苏启东肝癌防治所江苏启东

AIM In order to study the association betweenthe null genotypes of GSTM1 and GSTT1 and thegenetic susceptibility to hepatocellularcarcinoma(HCC).METHODS The genotypes of GSTM1 and GSTT1of 63 cases of HCC and 88 controls were detectedwith the multiple PCR *** The frequency of GSTM1 nullgenotype was 57.1% among the cases,and42.0% among the controls,the difference beingstatistically significant(χ~2=3.35,P=0.067),but χ~2 value approaching the significance *** odds ratio was 1.84(95% Cl= 0.91-3.37).The frequency of GSTT1 non-null genotype was87.3% among the cases and 52.5% among thecontrols,the difference being statisticallysignificant(χ~2=11.42,P=0.0007274).The oddsratio was 4.13(95% Cl=1.64-10.70).According to the cross analysis,the GSTT1 non-null genotype was more closely associated withHCC than GSTM1 null genotype,and these twofactors play an approximate additive interactionin the occurrence of *** The persons with GSTM1 nullgenotype and GSTT1 non-null genotype have theincreased risk to HCC.

关键词： liver neoplasms carcinoma hepatocellular GSTM1 GSTT1 null genotypes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The strategy of University Athletics DevelopmentIn China——From the university athletics Development pattern in jiangsu province

The strategy of University Athletics DevelopmentIn China——...

引用

第4届全国青年体育科学学术会议

作者： You Lai-ju Suzhou University of Science and Technology, Physical Culture Dept. Suzhou, jiangsu province

＜正＞Purpose of research: Owning to the gradual transformation and diversity of the system and mechanism of athletics in our country, universities are increasingly revealing their status and advantages in training athletic personnel. As a super-province both in economy and culture, jiangsu has the largest number of

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Equilibrium equations for nonlinear buckling analysis of drill-strings in 3D curved well-bores

引用

Science China(Technological Sciences) 2009年第3期52卷 590-595页

作者： TAN MeiLan1 & GAN LiFei2 1 Faculty of Science, jiangsu University, Zhenjiang 212013, China 2 94973 Unit in Hangzhou of Zhejiang province, Hangzhou 310021, China 1. Faculty of Science Jiangsu University Jiangsu Zhenjiang 212013 China 2. 94973 Unit in Hangzhou of Zhejiang province Hangzhou 310021 China

With the development of drilling technology, the oil/gas well has evolved from its early vertical straight form to the inclined, horizontal, plane curved, or even 3D curved well-bore. Understanding of the buck- ling behavior of a drill-string in a well-bore is crucial for the success of a drilling operation. Therefore, equilibrium equations for analyzing the buckling behavior of a drill-string in a 3D curved well-bore are required. Based on Love’s equilibrium equations for a curved and twisted rod in space, a set of equi- librium equations for the nonlinear buckling analysis of a drill-string in a 3D curved well-bore are de- rived by introducing a radial constraint of the well-bore. The proposed formulae can account for the well curvature and tortuosity. Thus, it can be used to analyze the buckling behaviors of a drill-string constrained in a well-bore and subjected to axial compression, torsion at its upper end, and gravity simultaneously. It is worth noting that the existing equations in the literature for a drill-string in a straight and plane curved well-bore with a constant curvature are a special case of the proposed model. Thus, the present model can provide a theoretical basis for the nonlinear buckling analysis of a drill-string constrained in a 3D curved well-bore.

关键词： nonlinear buckling tortuosity constraint equilibrium equations drill-strings 3D curved well-bores

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：