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Legume-specific SnRK1 promotes malate supply to bacteroids for symbiotic nitrogen fixation

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Molecular Plant 2023年第9期16卷 1396-1412页

作者： Da Guo Peng Liu Qianwen Liu Lihua Zheng Sikai Liu Chen Shen Li Liu Shasha Fan Nan Li jiangli dong Tao Wang College of Biological Sciences China Agricultural UniversityBejing 100193China

Nodulation is an energy-expensive behavior driven by legumes by providing carbon sources to bacteroids and obtaining nitrogen sources in return.The energy sensor sucrose nonfermenting 1-related protein kinase 1(SnRK1)is the hub of energy regulation in eukaryotes.However,the molecular mechanism by which SnRK1 coordinates the allocation of energy and substances during symbiotic nitrogen fixation(SNF)remains unknown.In this study,we identified the novel legume-specific SnRK1α4,a member of the SnRK1 family that positively regulates SNF.Phenotypic analysis showed that nodule size and nitrogenase activity increased in SnRK1α4-overexpressing plants and decreased significantly in snrk1α4 mutants.We demonstrated that a key upstream kinase involved in nodulation,Does Not Make Infection 2(DMI2),can phosphorylate SnRK1α4 at Thr175 to cause its activation.Further evidence clarified that SnRK1α4 phosphorylates the malate dehydrogenases MDH1/2 to promote malate production in the cytoplasm,supplying carbon sources to bacteroids.Therefore,our findings reveal an essential role of the DMI2–SnRK1α4–MDH pathway in supplying carbon sources to bacteroids for SNF and provide a new module for constructing cereal crops with SNF.

关键词： energy sensor SnRK1α4 phosphorylation malate anabolism nodulation Medicago truncatula

The histone methyltransferase SUVR2 promotes DSB repair via chromatin remodeling and liquidliquid phase separation

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Molecular Plant 2022年第7期15卷 1157-1175页

作者： Qianwen Liu Peng Liu Tuo Ji Lihua Zheng Chen Shen Shasha Ran Jinling Liu Yafei Zhao Yiding Niu Tao Wang jiangli dong State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193China Key Laboratory of Forage and Endemic Crop Biology Ministry of EducationSchool of Life SciencesInner Mongolia UniversityHohhotChina

Maintaining genomic integrity and stability is particularly important for stem cells,which are at the top of the cell lineage origin.Here,we discovered that the plant-specific histone methyltransferase SUVR2 maintains the genome integrity of the root tip stem cells through chromatin remodeling and liquid-liquid phase separation(LLPS)when facing DNA double-strand breaks(DSBs).The histone methyltransferase SUVR2(MtSUVR2)has histone methyltransferase activity and catalyzes the conversion of histone H3 lysine 9 monomethylation(H3K9me1)to H3K9me2/3 in vitro and in Medicago truncatula.Under DNA damage,the proportion of heterochromatin decreased and the level of DSB damage marker y-H2AX increased in suvr2 mutants,indicating that MtSUVR2 promotes the compaction of the chromatin structure through H3K9 methylation modification to protect DNA from damage.Interestingly,MtSUVR2 was induced by DSBs to phase separate and form droplets to localize at the damage sites,and this was confirmed by immunofluorescence and fluorescence recovery after photobleaching experiments.The IDR1 and lowcomplexity domain regions of MtSUVR2 determined its phase separation in the nucleus,whereas the IDR2 region determined the interaction with the homologous recombinase MtRAD51.Furthermore,we found that MtSUVR2 drove the phase separation of MtRAD51 to form"DNA repair bodies,"which could enhance the stability of MtRAD51 proteins to facilitate error-free homologous recombination repair of stem cells.Taken together,our study reveals that chromatin remodeling-associated proteins participate in DNA repair through LLPS.

关键词： stem cell droplets H3K9me2 SUVR2 Medicago truncatula

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豆科植物特异SnRK1调控共生固氮的机制

科学通报 2024年第2期69卷 137-139页

作者：郭达刘鹏董江丽王涛中国农业大学生物学院北京100193

豆科植物和根瘤菌之间的共生固氮(symbiotic nitrogen fixation,SNF)是高度耗能的代谢过程,植物每获取1 g固定的氮(N2)需要消耗5.2~18.8 g的碳源[1].尽管这一行为互利于双方,但共生固氮的有效进行依赖于豆科植物持续的碳源供应[2].豆科... 详细信息

豆科植物和根瘤菌之间的共生固氮(symbiotic nitrogen fixation,SNF)是高度耗能的代谢过程,植物每获取1 g固定的氮(N2)需要消耗5.2~18.8 g的碳源[1].尽管这一行为互利于双方,但共生固氮的有效进行依赖于豆科植物持续的碳源供应[2].豆科新型能量感受器GmNAS1/GmNAP1的发现,为设计高效利用作物周围环境中的碳源、提高共生固氮能力提供了理论支撑[3].然而,豆科植物在缺氮时如何分配能量和碳源实现高耗能共生固氮的分子机制尚不清楚[4].豆科植物是否有独特的能量感受器参与这一过程是科学家们一直探究的问题.

关键词：豆科植物共生固氮周围环境 NAP1 感受器根瘤菌代谢过程高效利用

Differentiation trajectories and biofunctions of symbiotic and un-symbiotic fate cells in root nodules of Medicago truncatula

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Molecular Plant 2022年第12期15卷 1852-1867页

作者： Qinyi Ye Fugui Zhu Fanghao Sun Tai-Cheng Wang Jiale Wu Peng Liu Chen Shen jiangli dong Tao Wang State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Grassland Science and Technology China Agricultural UniversityBeijing 100193China

The root nodule is a complex symbiotic nitrogen fixation factory,in which cells are highly heterogeneous.However,the differentiation trajectories and interconnection of nodule cells remain largely unknown.In this study,we set up a modified protocol for nodule protoplast preparation and performed a single-cell RNA sequencing profiling of the indeterminate Medicago truncatula nodule.We designated 13 cell clusters with specific expression patterns in 14-day post inoculation nodules and constructed a spatial and functional cellular map based on experimental data and bioinformatic analyses.Pseudotime analysis further revealed that two groups of apical meristematic cells develop into symbiotic and un-symbiotic fate cells along their particular trajectories.Biofunction analysis of each cell cluster revealed their particularity and interrelation,especially that the un-infected cells in nitrogen fixation zone are also involved in nitrogen assimilation by undertaking the asparagine synthesis.Collectively,our data offer an important resource for investigating the mechanism of nodule organogenesis and symbiotic nitrogen fixation.

关键词： single-cell RNA sequencing scRNA-seq nodule organogenesis symbiotic nitrogen fixation indeterminate nodule legume plant

The Chromosome-Level Genome Sequence of the Autotetraploid Alfalfa and Resequencing of Core Germplasms Provide Genomic Resources for Alfalfa Research

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Molecular Plant 2020年第9期13卷 1250-1261页

作者： Chen Shen Huilong Du Zhuo Chen Hongwei Lu Fugui Zhu Hong Chen Xiangzhao Meng Qianwen Liu Peng Liu Lihua Zheng Xiuxiu Li jiangli dong Chengzhi Liang Tao Wang State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural UniversityBeijingChina State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina

Alfalfa(Medicago sativa)is one of the most important forage crops in the world;however,its molecular genetics and breeding research are hindered due to the lack of a high-quality reference genome.Here,we report a de novo assembled 816-Mb high-quality,chromosome-level haploid genome sequence for‘Zhongmu No.1’alfalfa,a heterozygous autotetraploid.The contig N50 is 3.92 Mb,and 49165 genes are annotated in the genome.The alfalfa genome is estimated to have diverged from M.truncatula approximately 8 million years ago.Genomic population analysis of 162 alfalfa accessions revealed high genetic diversity,weak population structure,and extensive gene flow from wild to cultivated alfalfa.Genome-wide association studies identified many candidate genes associated with important agronomic traits.Furthermore,we showed that MsFTa2,a Flowering Locus T homolog,whose expression is upregulated in salt-resistant germplasms,may be associated with fall dormancy and salt resistance.Taken together,these genomic resources will facilitate alfalfa genetic research and agronomic improvement.

关键词： alfalfa genome assembly population gen etics GW AS Flowering Locus T

Resonance-assisted light–control–light characteristics of SnS_2 on a microfiber knot resonator with fast response

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Photonics Research 2018年第12期6卷 1137-1143页

作者： HUIHUI LU ZHONGMIN WANG ZHIJIN HUANG JUN TAO HANQING XIONG WENTAO QIU HEYUAN GUAN HUAZHUO dong jiangli dong WENGUO ZHU JIANHUI YU YONGCHUN ZHONG YUNHAN LUO JUN ZHANG ZHE CHEN Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications Department of Optoelectronic EngineeringJinan University Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes Jinan University

An all-optical light–control–light functionality with the structure of a microfiber knot resonator (MKR) coated with tin disulfide (SnS_2) nanosheets is experimentally demonstrated. The evanescent light in the MKR [with a resonance Q of ～59,000 and an extinction ratio (ER) of ～26 dB] is exploited to enhance light–matter interaction by coating a two-dimensional material SnS_2 nanosheet onto it. Thanks to the enhanced light–matter interaction and the strong absorption property of SnS_2, the transmitted optical power can be tuned quasi-linearly with an external violet pump light power, where a transmitted optical power variation rate ΔT with respect to the violet light power of ～0.22 dB∕mW is obtained. In addition, the MKR structure possessing multiple resonances enables a direct experimental demonstration of the relationship between resonance properties (such as Q and ER), and the obtained ΔT variation rate with respect to the violet light power. It verifies experimentally that a higher resonance Q and a larger ER can lead to a higher ΔT variation rate. In terms of the operating speed, this device runs as fast as ～3.2 ms. This kind of all-optical light–control–light functional structure may find applications in future all-optical circuitry, handheld fiber sensors, etc.

关键词： response fast experimental optical multiple pump can tin

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产前超声诊断胎儿食管重复囊肿1例

中国医学影像技术 2024年第3期40卷 473-474页

作者：董江里刘冬梅于佳丽欧阳湘南刘可萱林勤徐赣琼中南大学湘雅二医院超声诊断科湖南长沙410011 中南大学湘雅二医院超声影像医学研究室湖南长沙410011 湖南省医学影像临床医学研究中心湖南长沙410011 湖南省儿童医院超声科湖南长沙410007

孕妇28岁,孕1产0,外院疑诊胎儿食管闭锁,无特殊家族遗传病史。孕26周超声:胎儿生长发育与孕周相符,左心房后方紧邻食管边界清楚、形态不规则12.0mm×7.0mm囊性包块(图1A),其内未见血流信号,观察期间形态未见变化;考虑食管异常,食管闭锁... 详细信息

孕妇28岁,孕1产0,外院疑诊胎儿食管闭锁,无特殊家族遗传病史。孕26周超声:胎儿生长发育与孕周相符,左心房后方紧邻食管边界清楚、形态不规则12.0mm×7.0mm囊性包块(图1A),其内未见血流信号,观察期间形态未见变化;考虑食管异常,食管闭锁并食管气管瘘?孕27^(+1)周复查见包块增大至15.7mm×9.0mm,与气管或左右支气管未见明显交通(图1B),考虑食管重复囊肿(esophageal duplication cyst,EDC)。

关键词：胎儿食管囊肿超声检查,产前

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无座力炮动不平衡冲量测试方法研究

兵工自动化 2024年第6期43卷 36-39页

作者：贾琪邱奖利支凯艳杨江郭浩董长宏中国兵器工业试验测试研究院陕西渭南714200

针对无座力炮动不平衡冲量的测试存在操作过程繁琐且不易满足测量精度要求的问题,设计一种新的测试装置。通过低阻尼滑轨和高精度传感器测试出无座力炮发射时的速度、位移,根据动量原理算出无座力炮的动不平衡冲量。试验验证结果表明:... 详细信息

针对无座力炮动不平衡冲量的测试存在操作过程繁琐且不易满足测量精度要求的问题,设计一种新的测试装置。通过低阻尼滑轨和高精度传感器测试出无座力炮发射时的速度、位移,根据动量原理算出无座力炮的动不平衡冲量。试验验证结果表明:该测试系统平均测试误差为0.48%,可成功应用于无座力炮动不平衡冲量测试。

关键词：无座力炮动不平衡冲量低阻尼滑轨高精度传感器

Clinical efficacy and safety of linezolid in intensive care unit patients

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Journal of Intensive Medicine 2023年第1期3卷 65-72页

作者： Aijia Ma Meiling dong jiangli Cheng Xuelian Liao Wei dong Chang Liu Chenggong Hu Jing Yang Yan Kang Department of Critical Care Medicine West China Hospital of Sichuan UniversityChengduSichuan 610041China

Background:To characterize the population of critically ill patients and infections treated with linezolid in the intensive care unit(ICU),and to evaluate the clinical efficacy and safety of linezolid therapy.Methods::This multi-center,observational,real-world study was conducted across 52 hospitals between June 9,2018,and December 28,2019.Patients who met the following inclusion criteria were included:(1)admitted to the ICU,(2)of any age group,and(3)having a clinical or laboratory diagnosis of a Gram-positive bacterial infection.Clinical efficacy was categorized as success(cured or improved),failed,or non-evaluable.Adverse events and serious adverse events were recorded during treatment.Results::A total of 366 ICU patients who met the inclusion criteria were evaluated.Linezolid was used as second-and first-line treatment in 232(63.4%)and 134(36.6%)patients,respectively.The most common isolated strain was Staphylococcus aureus(methicillin-resistant Staphylococcus aureus:n=37/119,31.1%;methicillin-susceptible Staphylococcus aureus:n=15/119,12.6%);this was followed by Enterococci(vancomycin-resistant Enterococci:n=8/119,6.7%;vancomycin-susceptible Enterococci:n=11/119,9.2%)and Streptococcus pneumoniae(multidrug-resistant:n=4/119,3.4%;non-multidrug resistant:n=2/119,1.7%).The main infection sites where pathogens were detected included the lung(n=216/366,59.6%),skin and soft tissue(n=104/366,28.4%),and blood(n=50/366,13.7%).Clinical success was achieved in 301(82.2%)patients;34(9.3%)were cured and 267(73.0%)improved;treatment failure and non-evaluable outcomes were observed in 29(7.9%)in 36(9.8%)patients,respectively.Linezolid-related adverse events were reported in 8(2.2%)patients.No treatment-related serious adverse events were reported.Conclusions::Based on real-world results,linezolid was found to be effective and safe in the treatment of Gram-positive bacterial infections in critically ill patients.

关键词： Intensive care unit Linezolid Real-world study Gram-positive bacterial infection