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Defence Technology（防务技术） 2024年第4期34卷 69-77页

作者： Zhou Yu Li Xue Weidong Xu Jun liu Qi Jia jianghua Hu Jidong Wu National Key Laboratory of Lightning Protection and Electromagnetic Camouflage Army Engineering University Nanjing 210007JiangsuChina Unit 31608 of PLA Xiamen 361000FujianChina School of Biological Sciences&Medical Engineering Southeast UniversityNanjing 210096JiangsuChina

Brain functional networks model the brain's ability to exchange information across different regions,aiding in the understanding of the cognitive process of human visual attention during target searching,thereby contributing to the advancement of camouflage *** this study,images with various camouflage effects were presented to observers to generate electroencephalography(EEG)signals,which were then used to construct a brain functional *** topological parameters of the network were subsequently extracted and input into a machine learning model for *** results indicate that most of the classifiers achieved accuracy rates exceeding 70%.Specifically,the Logistic algorithm achieved an accuracy of 81.67%.Therefore,it is possible to predict target camouflage effectiveness with high accuracy without the need to calculate discovery *** proposed method fully considers the aspects of human visual and cognitive processes,overcomes the subjectivity of human interpretation,and achieves stable and reliable accuracy.

关键词： Camouflage effect evaluation Electroencephalography(EEG) Brain functional networks Machine learning

Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris

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Synthetic and Systems Biotechnology 2024年第1期9卷 26-32页

作者： Xueqin Lv Yuting Zhang Lingrui Wang Shixiu Cui Yanfeng liu jianghua Li Guocheng Du Long liu Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China Science Center for Future Foods Jiangnan UniversityWuxi 214122China Yixing Institute of Food Biotechnology Co. LtdYixing 214200China Jiaxing Institute of Future Food Jiaxing314015China Food Laboratory of Zhongyuan Jiangnan UniversityWuxi 214122China

Lactoferricin,a multifunctional peptide located in the N-terminal region of lactoferrin,has a broad-spectrum bacteriostatic *** is a promising candidate as a food additive and immune fortification agent and does not have the risks associated with drug residues and drug ***,we performed promoter and host cell screening to achieve the recombinant expression of lactoferricin in Pichia pastoris,showing an initial titer of 19.5 mg/L in *** X-33 using PAOX1 ***,we constructed a 0030-α hybrid signal peptide by fusing the 0030 signal peptide with the pro-sequence of α-factor secretory signal *** further increased the production of lactoferricin,with a titer of 28.8 mg/L in the fermentation supernatant in the shaking ***,we increased the expression of lactoferricin by fusing it with anionic antioxidant *** neutralization of positive charges yielded a titer of 55.3 mg/L in the shaking flask,and a highest titer of 193.9 mg/L in a 3-L *** antimicrobial activity analysis showed that recombinant-expressed lactoferricin exhibited potent antibacterial activity against Escherichia coli,Bacillus subtilis,and Staphylococcus *** study provides a reference for the construction of microbial cell factories capable of efficiently synthesizing antimicrobial peptides.

关键词： Antimicrobial peptide Lactoferricin Recombinant expression Pichia pastoris

Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch

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Synthetic and Systems Biotechnology 2024年第3期9卷 577-585页

作者： Haoyu Guo Rongzhen Tian Yaokang Wu Xueqin Lv jianghua Li Long liu a b Guocheng Du Jian Chen Yanfeng liu Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China Science Center for Future Foods Jiangnan UniversityWuxi214122China Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology Jiangnan UniversityWuxi214122China Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology Jiangnan UniversityWuxi214122China

Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic *** comparison to plasmid-based multi-copy expression,the utilization of chromosomal multi-copy genes offers increased stability of expression level,diminishes the metabolic burden on host cells,and enhances overall genetic *** this study,we developed the“BacAmp”,a stabilized gene integration expression and copy number amplification system for high-level expression in Bacillus subtilis,which was achieved by employing a combination of repressor and non-natural amino acids(ncAA)-dependent expression system to create a reversible switch to control the key gene recA for homologous *** the reversible switch is turned on,genome editing and gene amplification can be ***,the reversible switch was turned off therefore stabilizing the gene copy *** stabilized gene amplification system marked by green fluorescent protein,achieved a 3-fold increase in gene expression by gene amplification and maintained the average gene copy number at 10 after 110 *** we implemented the gene amplification system for the regulation of N-acetylneuraminic acid(NeuAc)synthesis,the copy number of the critical gene increased to an average of 7.7,which yielded a 1.3-fold NeuAc *** research provides a new avenue for gene expression in synthetic biology and can be applied in metabolic engineering in ***.

关键词： Bacillus subtilis Stabilized gene amplification system Homologous recombination recA

Exploring causality with biliary atresia at different levels:two-sample Mendelian randomization study

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World Journal of Pediatric Surgery 2024年第2期7卷 150-157页

作者： Shaowen liu Jiayinaxi Musha Zhiru Wang Xueting Wang Tengfei Li jianghua Zhan Clinical School of Paediatrics Tianjin Medical UniversityTianjinChina Department of General Surgery Tianjin Children's HospitalTianjinChina Urumqi City First People's Hospital(Urumqi Children's Hospital) UrumqiChina

Background In recent years,Mendelian randomization(MR)has been widely used to infer causality of related disease risk ***,this strategy has not been applied to biliary atresia(BA).Methods Genome-wide association studies(GWAS)data of 41 inflammatory cytokines,731 immune cell traits,and 1400 metabolites were obtained from public databases as exposure *** outcome information was obtained from a GWAS meta-analysis of 499 children with BA and 1928 normal *** variance weighting was the primary causality *** Q-test,MR-Egger intercept,MR pleiotropy residual sum and outlier,and'leave-one-out'analyses were used for sensitivity *** MR,MR-Steiger,and Linkage Disequilibrium Score were used to exclude the effects of reverse causality,genetic association,and linkage *** MR results showed that a total of seven traits had potential causal relationships with BA,including three inflammatory cytokines:eotaxin(odds ratio(OR)=1.45,95%confidence interval(CI):1.08 to 1.95,pFDR=0.18),G-CSF(OR=4.21,95%CI:1.75 to 10.13,pFDR=0.05)and MCP-1/MCAF(OR=1.53,95%CI:1.12 to 2.10,pFDR=0.14);three immune cell traits:CD8dim NKT/T cells ratio(OR=0.59,95%CI:0.45 to 0.77,pFDR=0.06),CD8dim NKT counts(OR=0.58,95%CI:0.43 to 0.78,pFDR=0.06),CD8dim NKT/lymphocyte ratio(OR=0.63,95%CI:0.49 to 0.81,pFDR=0.06);one metabolite:X-12261 levels(OR=2.86,95%CI:1.73 to 4.74,pFDR=0.06).Conclusions In this study,eotaxin,G-CSF,MCP-1/MCAF,and X-12261 levels were shown to be risk factors for ***,CD8dim NKT/T cells ratio,CD8dim NKT counts,and CD8dim NKT/lymphocyte ratio were protective factors for *** findings provided a promising genetic basis for the etiology,diagnosis,and treatment of BA.

关键词： protective analysis. diagnosis

Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates

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Environmental Science and Ecotechnology 2024年第5期21卷 396-404页

作者： Yan Zhang Yu Qiu Kai liu Wenjun Zhong jianghua Yang Florian Altermatt Xiaowei Zhang State Key Laboratory of Pollution Control&Resource Reuse School of the EnvironmentNanjing UniversityNanjing210023China Department of Evolutionary Biology and Environmental Studies University of ZurichZürichSwitzerland Department of Aquatic Ecology Eawag:Swiss Federal Institute of Aquatic Science and TechnologyDübendorfSwitzerland Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture and Rural AffairsFreshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxi214081China

The monitoring and management of aquatic ecosystems depend on precise estimates of *** analyses of environmental nucleic acids(eNAs),including environmental DNA(eDNA)and environmental RNA(eRNA),have garnered attention for their cost-effective and non-invasive biomonitoring ***,the accuracy of biodiversity estimates obtained through eNAs can vary among different organismal *** we evaluate the performance of eDNA and eRNA metabarcoding across nine organismal groups,ranging from bacteria to terrestrial vertebrates,in three crosssections of the Yangtze River,*** observe robust complementarity between eDNA and eRNA *** relative detectability of eNAs was notably influenced by major taxonomic groups and organismal sizes,with eDNA providing more robust signals for larger *** eDNA and eRNA exhibited similar cross-sectional and longitudinal ***,the detectability of larger organisms declined in eRNA metabarcoding,possibly due to differential RNA release and decay among different organismal groups or *** underscoring the potential of eDNA and eRNA in large river biomonitoring,we emphasize the need for differential interpretation of eDNA versus eRNA *** highlights the importance of careful method selection and interpretation in biomonitoring studies.

关键词： Environmental nucleic acids Aquatic biodiversity Organismal size Species detectability

A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger

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Synthetic and Systems Biotechnology 2024年第2期9卷 209-216页

作者： Yangyang Li Cen Li Yishan Fu Quan Zhang Jianing Ma Jingwen Zhou jianghua Li Guocheng Du Song liu National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing Jiangnan UniversityWuxi214122China Science Center for Future Foods Jiangnan UniversityWuxi214122China School of Biotechnology Jiangnan University1800 Lihu RoadWuxi214122China Jiangsu Provisional Research Center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Life Sciences/Institute of Agro-bioengineeringGuizhou UniversityGuiyang550025China Dalian Research Institute of Petroleum and Petrochemicals SINOPECDalian116000China School of Chemical Engineering Dalian University of TechnologyDalian116000China

Aspergillus niger is a highly versatile fungal strain utilized in industrial *** expression levels of recombinant genes in *** can be enhanced by increasing the copy ***,given the prolonged gene editing cycle of ***,a“one-step”strategy facilitating the simultaneous integration of recombinant genes into multiple genomic loci would provide a definitive *** our previous study,a visual multigene editing system(VMS)was designed to knock out five genes,employing a tRNA-sgRNA array that includes the pigment gene albA and the target *** upon this system,hybrid donor DNAs(dDNAs)were introduced to establish a clustered regularly interspaced short palindromic repeats(CRISPR)-based multiplex integration ***,a CRISPR-Cas9 homology-directed repair(CRISPR-HDR)system was constructed in *** by co-transforming the CRISPR-Cas9 plasmid(with a highly efficient sgRNA)and the dDNA,resulting in precise integration of recombinant xylanase gene xynA into the target loci(theβ-glucosidase gene bgl,the amylase gene amyA,and the acid amylase gene ammA).Subsequently,the length of homology arms in the dDNA was optimized to achieve 100%editing efficiency at each of the three gene *** achieve efficient multiplex integration in ***,the CRISPR plasmid pLM2 carrying a sgRNA-tRNA array was employed for concurrent double-strand breaks at multiple loci(bgl,amyA,ammA,and albA).Hybrid dDNAs were then employed for repair,including dDNA1-3(containing xynA expression cassettes without selection markers)and dDNAalbA(for albA knockout).Among the obtained white colonies(RLM2′),23.5%exhibited concurrent replacement of the bgl,amyA,and ammA genes with xynA(three copies).Notably,the xynA activity obtained by simultaneous insertion into three loci was 48.6%higher compared to that obtained by insertion into only the bgl ***,this multiple integration toolkit successfully enhanced the expression of endogenous pectinase pelA and

关键词： Multiplex integration Aspergillus niger CRISPR-Cas9 homologous direct repair White colony

Ultrahigh-brightness 50 MeV electron beam generation from laser wakefield acceleration in a weakly nonlinear regime

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Matter and Radiation at Extremes 2024年第3期9卷 4-10页

作者： Zhongtao Xiang Changhai Yu Zhiyong Qin Xuhui Jiao Jiahui Cheng Qiaoxuan Zhou Gatie Axi jianghua Jie Ya Huang Jintan Cai Jiansheng liu Department of Physics Shanghai Normal UniversityShanghai 200234China

We propose an efficient scheme to produce ultrahigh-brightness tens of MeV electron beams by designing a density-tailored plasma to induce a wakefield in the weakly nonlinear regime with a moderate laser energy of 120 *** this scheme,the second bucket of the wakefield can have a much lower phase velocity at the steep plasma density down-ramp than the first bucket and can be exploited to implement longitudinal electron injection at a lower laser intensity,leading to the generation of bright electron beams with ultralow emittance together with low energy ***-dimensional particle-in-cell simulations are carried out and demonstrate that high-quality electron beams with a peak energy of 50 MeV,ultralow emittance of28 nm rad,energy spread of 1%,charge of 4.4 pC,and short duration less than 5 fs can be obtained within a 1-mm-long tailored plasma density,resulting in an ultrahigh six-dimensional brightness B6D,n of2×1017 A/m2/0.1%.By changing the density parameters,tunable bright electron beams with peak energies ranging from 5 to 70 MeV,a small emittance of B0.1 mm mrad,and a low energy spread at a few-percent level can be *** bright MeV-class electron beams have a variety of potential applications,for example,as ultrafast electron probes for diffraction and imaging,in laboratory astrophysics,in coherent radiation source generation,and as injectors for GeV particle accelerators.

关键词： weakly nonlinear acceleration

High glucose promotes macrophage M1 polarization through miR-32/Mef2d/cAMP signaling pathway

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Genes & Diseases 2024年第2期11卷 539-541页

作者： Cong Chen Jingsong Cao Yan Gao Xiaoping Xie Zhibo Zhao Qing Yuan Yuqi He Xuyu Zu jianghua liu The First Affiliated Hospital Institute of Endocrinology and MetabolismCenter for Clinical Research in DiabetesHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China The First Affiliated Hospital Department of Laboratory MedicineHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China The First Affiliated Hospital Institute of OncologyHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China

Chronic inflammation is a crucial inducerof diabetesvascular *** is that high glucose easily induces macrophage activation.1 Macrophages are the principal participants in innate immunity and exist in all human *** pathological vascular,infiltrated macrophages secrete inflammatory factors leading to an increase in plaque stability.?In macrophage polarization,autophagy plays an important *** of macrophage autophagy could induce macrophage polarization from the M1 phenotype to M2 phenotype and inhibit inflammatory reactions.3 Our previous research found that high glucose condition promotes miR-32 expression and macrophage M1 polarization,4 but the mechanism of miR-32 promoting macrophage M1 polarization is *** this study,we found that,under a high-glucose condition,miR-32/Mef2d/cAMP signaling promoted M1 macrophage polarization via inhibited *** results provide a theoretical and experimental basis for the prevention and treatment of T2D vascular inflammation.

关键词： macrophage miR prevention

Clinical characteristics and outcomes of hospitalized kidney transplant recipients with COVID-19 infection in China during the Omicron wave:a single-center cohort study

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第6期25卷 529-540页

作者： Duo LV Xishao XIE Qinyun YANG Zhimin CHEN Guangjun liu Wenhan PENG Rending WANG Hongfeng HUANG jianghua CHEN Jianyong WU Department of Clinical Pharmacy the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Zhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research Hangzhou 310003China Kidney Disease Center the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Key Laboratory of Kidney Disease Prevention and Control Technology Zhejiang ProvinceHangzhou 310003China Institute of Nephrology Zhejiang UniversityHangzhou 310003China Zhejiang Clinical Research Center of Kidney and Urinary System Disease Hangzhou 310003China The Third Grade Laboratory under the National State Administration of Traditional Chinese MedicineHangzhou 310003China Information Center the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Background:Following the short-term outbreak of coronavirus disease 2019(COVID-19)in December 2022 in China,clinical data on kidney transplant recipients(KTRs)with COVID-19 are ***:We conducted a single-center retrospective study to describe the clinical features,complications,and mortality rates of hospitalized KTRs infected with COVID-19 between Dec.16,2022 and Jan.31,*** patients were followed up until Mar.31,***:A total of 324 KTRs with COVID-19 were *** median age was 49 *** median time between the onset of symptoms and admission was 13 ***,azvudine,and nirmatrelvir/ritonavir were administered to 67(20.7%),11(3.4%),and 148(45.7%)patients,***-nine(9.0%)patients were treated with more than one antiviral ***-eight(14.8%)patients were treated with tocilizumab and 53(16.4%)patients received baricitinib *** acute kidney injury(AKI)occurred in 81(25.0%)patients and 39(12.0%)patients were admitted to intensive care *** infections were observed in 55(17.0%)***(15.4%)patients lost their *** 28-d mortality rate of patients was 9.0%and 42(13.0%)patients died by the end of *** Cox regression analysis identified that cerebrovascular disease,AKI incidence,interleukin(IL)-6 level of>6.8 pg/mL,daily dose of corticosteroids of>50 mg,and fungal infection were all associated with an increased risk of death for hospitalized ***:Our findings demonstrate that hospitalized KTRs with COVID-19 are at high risk of *** administration of immunomodulators or the late application of antiviral drugs does not improve patient survival,while higher doses of corticosteroids may increase the death risk.

关键词： Coronavirus disease 2019(COVID-19) Kidney transplant Hospitalization Mortality