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Development and Application of an RT-PCR Method for Differentiation of PEDV Variant Strains and Classical Strains

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Animal Husbandry and Feed Science 2015年第1期7卷 21-24,60页

作者： Qin Yibin Lu Bingxia Zhao Wu He Ying Li Yingying Li Bin Liang Jiaxing Liang Baozhong Su Qianlian jiang dongfu Zhou Yingning Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Animal Vaccines and New Technology College of Animal Science and Technology Guangxi University

In order to establish a rapid RT-PCR assay for detection of porcine epidemic diarrhea virus（PEDV）,a pair of special primers was designed based on S gene sequence of PEDV published in Gen *** optimization of the reaction system,a rapid RT-PCR method was *** showed that a fragment of 826 bp was successfully amplified only from the variant PEDV by RT-PCR,while the expected target fragment could not be amplified from TGEV,porcine rotavirus,porcine kobuvirus,PRRSV,PRV,CSFV,*** test of RT-PCR indicated that only 11.3 pg nuclear acids could be detected accurately and rapidly.A total of 123 samples collected from different farms in Guangxi Province were detected by the established RT-PCR,the positive rate of PEDV was 67.5%,and the positive rate of new PEDV was 86.7%（72/83）.Therefore,the RT-PCR could be used as an effective tool for differentiating diagnosis of the highly pathogenic PEDV in epidemiological investigations.

关键词： PEDV RT-PCR Differential diagnosis

A Comprehensive Framework for Decoding Salty Taste Information from EEG Signals: Distinguishing Brain Reactions to Saltiness of Comparable Intensity

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Food Science and Human Wellness 2024年

作者： Jingjing Liu Yifei Xu Xingwang Lian Tianming Liu Haohao Ning Xi jiang Shixin Yu Shikun Liu Lu Huang Xiaojun Sun jiangyong Li dongfu Xu School of Life Science and Technology， University of Electronic Science and Technology of China Department of Computer Science， the University of Georgia School of Automation Engineering， Northeast Electric Power University

The perception of salty taste is crucial for individuals to make healthy food choices. Yet， the brain electrophysiological signals underlying salty taste perception have been poorly described. In this study， electroencephalography (EEG) was used to record brain activity induced by NaCl solution as a salty taste stimulus. A combination of a custom delivery device and stimulation paradigm was employed to preserve the salty taste signal clearly. A stimulus-response capture method was proposed that could adapt to individual differences in brain responses to salty taste and accurately segment salty taste response signals. Applying this method to the EEG processing workflow can form a complete data processing framework. The results showed that the neural response induced by salty taste reached a high activity level in the initial stage within a short period (0.2s)， and there was a sustained periodic response within 0.75s after the stimulation. Moreover， the salty taste information in the EEG signal was decoded， and discrimination of two similar concentrations of salty taste solutions was achieved far above the chance level (average identification rate: 89.66%). This study demonstrated experimental paradigms and research methods for understanding salty taste perception， which could provide references for research on other basic tastes.

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Detection and Analysis of Routine Blood Indexes in Guike Pig

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Agricultural Biotechnology 2018年第3期7卷 95-98,103页

作者： Qianlian SU Bin LI Jiaxing LIANG Yingning ZHOU Zhongwei CHEN Yibin QIN Bingxia LU Ying HE Qunpeng DUAN dongfu jiang Jingzhuan LU Wu ZHAO Guangxi Veterinary Research Institute/Guangxi Key kahoratory of Veterinary Biotechnology Nanning 530001 China

[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexe

关键词： Guike pig Landrace pig Enshi black pig Routine blood indexes Detection and analysis

纳米复合W-Cu FGM的致密性及界面结合特征

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粉末冶金材料科学与工程 2016年第2期21卷 326-332页

作者：蒋冬福范景莲刘涛田家敏中南大学粉末冶金国家重点实验室长沙410083

以溶胶-喷雾干燥-热还原制备的纳米晶W-Cu复合粉末为原料,通过球磨改性、叠层压制和一步液相烧结分别制备3种两层梯度复合细晶W-Cu材料(W-10Cu/W-30Cu,W-20Cu/W-30Cu和W-30Cu/W-50Cu),对其致密度、组织成分特征及界面结合性能进行研究... 详细信息

以溶胶-喷雾干燥-热还原制备的纳米晶W-Cu复合粉末为原料,通过球磨改性、叠层压制和一步液相烧结分别制备3种两层梯度复合细晶W-Cu材料(W-10Cu/W-30Cu,W-20Cu/W-30Cu和W-30Cu/W-50Cu),对其致密度、组织成分特征及界面结合性能进行研究与分析。结果表明:3种梯度材料各均质层都达到高致密(相对密度>98%);梯度材料具有明显的梯度组织,界面结合完好,Cu相呈连续网状结构,包裹在均匀分布的细小W晶粒周围;成分呈阶梯式变化,各层成分因Cu相的迁移和流失与初始设计值有一定的偏差;材料力学性能呈现梯度性,界面显微硬度处在两层显微硬度之间,结合强度高于各自富Cu层的拉伸强度,表明纳米复合W-Cu功能梯度材料各成分层之间有着优良的结合性。

关键词：纳米W-Cu 梯度材料致密性成分变化界面结合性结合强度

维普期刊数据库

Phylogenetic Analysis of HN Gene of Eight Pigeon NDV Isolates in Guangxi

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Animal Husbandry and Feed Science 2018年第2期10卷 136-138页

作者： Lu Bingxia Liang Jiaxing Duan Qunpeng Chen Zhongwei jiang dongfu Lu Jingzhuan Zhou Yingning Bi Bingfen He Ying Qin Yibin Li Bin Su Qianlian Zhao Wu Guangxi Key Laboratory of Veterinary Biotechnology Guangxi Veterinary Research Institute

[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease（ND）.[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and *** molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino *** belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent *** of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was *** were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV.

关键词： Pigeon Newcastle disease virus (NDV) HN gene Phylogenetic analysis

Detection of Porcine Epidemic Diarrhea Virus in Guangxi Province from 2011 to 2014 and Sequence Analysis of Its M Gene

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Animal Husbandry and Feed Science 2016年第1期8卷 12-17,38页

作者： Lu Bingxia Qin Yibin He Ying Li Yingying Liang Jiaxing Li Keyu Li Bin Su Qianlian Zhou Yingning jiang dongfu Lu Jingzhuan Bi Bingfen Liang Baozhong Duan Qunpeng Zhao Wu Guangxi Key Laboratory of Animal Vaccines and New Technology Guangxi Veterinary Research Institute College of Animal Science and Technology Guangxi University

Detection of pigs epidemic diarrhea virus （PEDV） was conducted on 331 piglets diarrhea fecal samples collected in Nanning, Yulin and other 12 areas of Guangxi Province from January of 2011 to April of 2014 by the method of reverse transcription-polymerase chain reaction （RT-PCR）. The results showed that the positive samples of PEDV were 210 and the positive rate was 63.44%. The clone and sequencing of M gene was carried out on 25 positive samples. PEDV reference strains were selected from GeneBank to conduct the sequence homology alignment analysis and the phylogenetic tree of M gene. The M gene homology and amino acid sequence identity between 25 isolated strains and 51 reference strains were 96.0% - 99.6% and 94.3% - 99.6%, respectively. The genetic variation anal- ysis of M gene showed that the genetic relationship of PEDV prevalent strains in Guangxi Province from 2013 to 2014 was close to that of the prevalent strains in Bei- jing, Anhui, Wuhan, Hebei and Guangdong from 2010 to 2013, and which were far from that of the Chinese early isolates CH/S （GenBank number： JN547228 ）, vaccine strain CV777 （GenBank number： AF353511 ） and Attenuated DR13 （GenBank number： JQ023162）. Indicating that the PEDV strains prevalent in Guan- gxi in recent years showed significant variation with the early isolates.

关键词： Porcine epidemic diarrhea virus (PEDV) M gene Genetic variation

Isolation of Swine Corynebacteria and Analysis of 16S rDNA Gene

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Animal Husbandry and Feed Science 2016年第6期8卷 339-343页

作者： Qin Yibin He Pingping Lu Bingxia He Ying Li Bin Liang Jiaxing Duan Qunpeng Chen Zhongwei Bi Bingfen Zhou Yingning Su Qianlian jiang dongfu Li Keyu Lu Jingzhuan Zhao Wu Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Animal Vaccines and New Technology Nanning 530001 China Guangxi Institute of Fishery Sciences Nanning 530021 China College of Animal Science and Technology Guangxi University Nanning 530005 China

A strain of gram-positive bacillus was isolated from suppurative lung organs of nursery pigs in a pig farm, which was further characterized by morphological observation, cultivation test, biochemical test, drug sensitivity test, pathogenicity test and 16S rDNA gene cloning and sequence analysis. The results showed that the isolate grew well in rabbit blood agar plate and horse serum tryptone soybean agar （TSA） plate under aerobic condition, which could lead to mortality of mice and were susceptible to cephalosporin antibiotics and fluoroquinolone antibiotics. Phylogenetic analysis showed that the isolate had close genetic evolutionary relationships with Corynebacterium bacteria, and the sequence of 16S rRNA gene shared the homology of 91.7% -98.3% with the representative strain of coryne- bacteria, indicating the isolated strain was eorynebactefium.

关键词： Coryrtebacterium Isolation and identification 16S rRNA Gene analysis

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贞苓增免散对罗非鱼的安全性评价

中国畜牧兽医 2021年第11期48卷 4004-4013页

作者：何文娜肖婷何颖周英宁蒋冬福卢敬专陈忠伟胡庭俊广西大学动物科学技术学院南宁530004 广西壮族自治区兽医研究所广西兽医生物技术重点实验室南宁530001

试验旨在确定贞苓增免散在临床推荐剂量下对罗非鱼的安全性,研究罗非鱼使用剂量安全范围并综合评价药物的疗效,为临床合理用药提供重要依据与指导。试验分别设置空白组(基础饲粮)、1倍剂量组(含贞苓增免散0.4%)、3倍剂量组(含贞苓增免散... 详细信息

试验旨在确定贞苓增免散在临床推荐剂量下对罗非鱼的安全性,研究罗非鱼使用剂量安全范围并综合评价药物的疗效,为临床合理用药提供重要依据与指导。试验分别设置空白组(基础饲粮)、1倍剂量组(含贞苓增免散0.4%)、3倍剂量组(含贞苓增免散1.2%)、5倍剂量组(含贞苓增免散2.0%)和10倍剂量组(含贞苓增免散4.0%)共5个组。连续饲喂30 d,分别在第15和30天检测罗非鱼的临床体征、血液生理生化指标、器官指数,并进行组织病理学观察。结果表明,各剂量组罗非鱼活动、摄食、体色、排泄等均良好,与空白组罗非鱼没有明显区别。0~15 d,增重率和特定生长率在1倍剂量组均最高,且均显著或极显著高于空白组(P<0.05;P<0.01),但其随着贞苓增免散剂量增加而减少;15~30 d贞苓增免散各剂量组增重率均极显著高于空白组(P<0.01)。各剂量组罗非鱼的血液生理生化指标中部分指标与空白组相比有显著差异,但均在正常范围内。0~15 d时各剂量组和15~30 d时1倍剂量组的罗非鱼脾脏指数均显著高于空白组(P<0.05)。显微镜下观察各组罗非鱼肝脏和脾脏组织切片,均未发现药物引起的细胞毒性变化。以上结果说明贞苓增免散在10倍推荐剂量及以下连续饲喂罗非鱼30 d具有较好的安全性,将其作为饲料添加剂能提高罗非鱼生长性能、增强免疫力,对推动罗非鱼养殖快速高效发展具有重要意义。

关键词：贞苓增免散罗非鱼生理指标生化指标

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文山松毛虫肠道微生物区系及其感染CPV后的变化

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中国生物防治学报 1998年第1期14卷 49-50页

作者：陈世维陈尔厚索启恒段兆尧胡光辉江东福马萍钱家康云南省微生物所云南省林科院

文山松毛虫肠道微生物区系及其感染ＣＰＶ后的变化①陈世维陈尔厚索启恒段兆尧胡光辉江东福马萍钱家康（云南省林科院，昆明６５０２０４）（云南省微生物所，昆明６５０２０１）ＩＮＴＥＳＴＩＮＡＬＭＩＣＲＯＦＬＯＲＡＯＦＤＥＮＤ... 详细信息

文山松毛虫肠道微生物区系及其感染ＣＰＶ后的变化①陈世维陈尔厚索启恒段兆尧胡光辉江东福马萍钱家康（云南省林科院，昆明６５０２０４）（云南省微生物所，昆明６５０２０１）ＩＮＴＥＳＴＩＮＡＬＭＩＣＲＯＦＬＯＲＡＯＦＤＥＮＤＲＯＬＩＭＵＳＰＵＮＣＴＡＴＵＳＷ...

关键词：文山松毛虫肠道微生物区系肠胃微生物区系 CPV