Phylogenetic Analysis of HN Gene of Eight Pigeon NDV Isolates in Guangxi

作     者：Lu Bingxia Liang Jiaxing Duan Qunpeng Chen Zhongwei Jiang Dongfu Lu Jingzhuan Zhou Yingning Bi Bingfen He Ying Qin Yibin Li Bin Su Qianlian Zhao Wu 

作者机构：Guangxi Key Laboratory of Veterinary BiotechnologyGuangxi Veterinary Research Institute 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2018年第10卷第2期

页      面：136-138页

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by Science and Technology Major Projects of Guangxi Province(GKAA17204057) Systematic Research Project of Guangxi Key Laboratory of Veterinary Biotechnology(14-045-31-A-5) Fundamental Research Fund of Guangxi Veterinary Research Institute(GKZX 2016-2) 

主　　题：Pigeon Newcastle disease virus (NDV) HN gene Phylogenetic analysis 

摘      要：[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease（ND）.[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and *** molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino *** belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent *** of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was *** were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV.