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用磁偏转SIMS实现超高分辨力的分析

真空科学与技术 1998年第A12期18卷 83-86页

作者： jiang,zx

白细胞介素6对人冠状动脉平滑肌细胞基质金属蛋白酶系统分泌平衡的影响

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中国组织工程研究与临床康复 2007年第41期11卷 8271-8275页

作者：李丽张清华蒋知新高德禄谢声扬李安全南方医科大学广东省广州市510515 解放军第三○五医院心血管内科北京市100017 解放军第三○五医院老年病中心实验室北京市100017 解放军第三○五医院检验科北京市100017

目的:研究表明,白细胞介素6等多种炎症因子与急性冠状动脉综合征斑块的稳定性下降有着密切联系;金属蛋白酶组织抑制因子的分泌失衡,也可能是斑块稳定性的原因之一。实验拟证明白细胞介素6对人冠状动脉平滑肌细胞的基质金属蛋白酶3及金... 详细信息

目的:研究表明,白细胞介素6等多种炎症因子与急性冠状动脉综合征斑块的稳定性下降有着密切联系;金属蛋白酶组织抑制因子的分泌失衡,也可能是斑块稳定性的原因之一。实验拟证明白细胞介素6对人冠状动脉平滑肌细胞的基质金属蛋白酶3及金属蛋白酶组织抑制因子1分泌平衡的影响。方法:实验于2006-12/2007-04在解放军第三○五医院老年病中心实验室完成。①实验材料:人冠状动脉平滑肌细胞(Casecade公司);白细胞介素6(PeProtech公司)。②实验过程:培养人冠状动脉平滑肌细胞,传代至5～7代。一期:分别加入0和10μg/L白细胞介素6(0μg/L为对照组)刺激人冠状动脉平滑肌细胞,分别孵育2,4,8,24,36h,收集细胞培养液上清。二期:分别采用0,5,10,50μg/L白细胞介素6刺激人冠状动脉平滑肌细胞(0μg/L为对照组),6h后收集细胞培养液上清。③实验评估:应用酶联接免疫吸附剂测定方法检测细胞培养液上清内基质金属蛋白酶3、金属蛋白酶组织抑制因子1的表达量。结果:①加入白细胞介素6后,基质金属蛋白酶3、金属蛋白酶组织抑制因子1的表达量在2h时开始增加,并随时间的延长而不断增加;随着白细胞介素6剂量的增加,表达量均不断增加。②基质金属蛋白酶3/金属蛋白酶组织抑制因子1在2h开始升高,8h达到顶峰,而后缓慢下降,与对照组比较,P均<0.05;随着白细胞介素6剂量的增加,表达量均不断升高,10μg/L和50μg/L组与对照组比较,P<0.05。结论:白细胞介素6可使人冠状动脉平滑肌细胞分泌不稳定斑块标记物--基质金属蛋白酶3、金属蛋白酶组织抑制因子1及基质金属蛋白酶3/金属蛋白酶组织抑制因子1增加,且呈剂量和时间依赖效应,说明炎症可能是急性冠状动脉综合征发生发展的机制之一。

关键词：冠状动脉疾病炎症白细胞介素6 基质溶解素1 金属蛋白酶类组织抑制剂组织构建

Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He Li Li Sai Wang Shao-jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

Toward understanding the role of genomic repeat elements in neurodegenerative diseases

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Neural Regeneration Research 2025年第3期20卷 646-659页

作者： Zhengyu An Aidi jiang Jingqi Chen Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina MOE Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence Fudan UniversityShanghaiChina Zhangjiang Fudan International Innovation Center ShanghaiChina

Neurodegenerative diseases cause great medical and economic burdens for both patients and society;however, the complex molecular mechanisms thereof are not yet well understood. With the development of high-coverage sequencing technology, researchers have started to notice that genomic repeat regions, previously neglected in search of disease culprits, are active contributors to multiple neurodegenerative diseases. In this review, we describe the association between repeat element variants and multiple degenerative diseases through genome-wide association studies and targeted sequencing. We discuss the identification of disease-relevant repeat element variants, further powered by the advancement of long-read sequencing technologies and their related tools, and summarize recent findings in the molecular mechanisms of repeat element variants in brain degeneration, such as those causing transcriptional silencing or RNA-mediated gain of toxic function. Furthermore, we describe how in silico predictions using innovative computational models, such as deep learning language models, could enhance and accelerate our understanding of the functional impact of repeat element variants. Finally, we discuss future directions to advance current findings for a better understanding of neurodegenerative diseases and the clinical applications of genomic repeat elements.

关键词： Alzheimer's disease ataxia deep learning long-read sequencing neurodegeneration neurodegenerative diseases Parkinson's disease repeat element structural variant

Gut microbiota shifts in hepatitis B-related portal hypertension after transjugular intrahepatic portosystemic shunt:Mechanistic and clinical implications

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World Journal of Gastroenterology 2025年第3期31卷 134-137页

作者： Qi-Rong jiang Da-Wu Zeng Department of Hepatology Hepatology Research InstituteThe First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina Department of Infectious Diseases The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350004Fujian ProvinceChina

In this article,we provide commentary on the recent article by Zhao et *** focus on the shifts in the gut microbiota of patients with hepatitis B virus(HBV)-associated cirrhosis/portal hypertension(PH)following transjugular intrahepatic portosystemic shunt(TIPS)and the implications for understanding the mechanisms,diagnosis,and *** comparing the gut microbiota composition and dynamic changes before and after TIPS in patients with and without hepatic encephalopathy,the authors found an increase in non-probiotic bacteria in those who developed hepatic encephalopathy post-TIPS,with Morganella species present only in the hepatic encephalopathy *** gut microbiota changes post-TIPS among patients without the occurrence of hepatic encephalopathy suggest potential therapeutic benefits through prophylactic microbiome ***,the specific gut microbiota alterations may hold promise to predict the risk of hepatic encephalopathy in individuals undergoing TIPS for HBVrelated *** these promising findings,future studies are needed to address limitations,including a small sample size,a relatively short evaluation period for gut microbiota alterations,the absence of data on dynamic alterations in gut microbiota post-TIPS and their correlation with blood ammonia levels,and the lack of validation in animal *** conclusion,Zhao et al's study has shed new light on the link of gut microbiota with post-TIPS hepatic encephalopathy,potentially through the intricate gut-liver axis,and has important clinical implications for improving the management of patients with HBV-related PH.

关键词： Hepatitis B virus Cirrhosis Portal hypertension Hepatic encephalopathy Transjugular intrahepatic portosystemic shunt Gut microbiota

人冠状动脉平滑肌细胞表达基质金属蛋白酶3与白细胞介素1β的相关性

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中国组织工程研究与临床康复 2007年第14期11卷 2617-2620页

作者：张清华夏爱祥蒋知新黄平方效民王志津宋成运赵婷解放军第三○五医院老年病中心北京市100017 解放军第三○五医院中心实验室北京市100017 解放军第三○五医院内六科北京市100017

目的:观察人冠状动脉平滑肌细胞表达基质金属蛋白酶3与炎症因子白细胞介素1β的关系。方法:实验于2005-05/2006-08在解放军第三○五医院老年病中心实验室完成。人冠状动脉平滑肌实验用5～7代的细胞,按2.5×108L-1密度接种于25cm2的培养... 详细信息

目的:观察人冠状动脉平滑肌细胞表达基质金属蛋白酶3与炎症因子白细胞介素1β的关系。方法:实验于2005-05/2006-08在解放军第三○五医院老年病中心实验室完成。人冠状动脉平滑肌实验用5～7代的细胞,按2.5×108L-1密度接种于25cm2的培养瓶中,24h后换液,生长接近80%融合时进行如下处理:①20μg/L质量浓度白细胞介素1β培养0,2,4,8,24,36h后收集上清夜和细胞。②不同质量浓度(0,5,20,40μg/L)白细胞介素1β处理6h后收集上清液和细胞。采用细胞总RNATRIzol试剂提取RNA,在紫外分光光度计确定总RNA的浓度和纯度,并在12g/L的琼脂糖凝胶中电泳,凝胶成像分析RNA的质量。应用实时荧光定量聚合酶链反应的方法检测细胞内基质金属蛋白酶3基因的表达量。结果:①细胞总RNA质量及纯度:每份样品取4μL稀释至400μL测定其A260nm/A280nm吸光度比值均在1.8～2.0之间,说明所提RNA纯度较高;电泳图片上可见RNA清晰的18S和28S的条带,无弥散,说明RNA质量较好。②目的基因的表达量:同剂量白细胞介素1β作用下,基质金属蛋白酶3基因的表达量在2h时开始发生上调,8h达高峰,而后开始下降;在不同剂量白细胞介素1β作用下,基质金属蛋白酶3基因的表达量在实验剂量范围内随着白细胞介素1β的剂量加大呈上升趋势(r=0.904,P=0.000),20μg/L和40μg/L白细胞介素1β作用下,基质金属蛋白酶3基因的表达量差异无显著性(P=0.154),其余各剂量间基质金属蛋白酶3基因的表达量差异均有显著性(P<0.05)。结论:炎症因子白细胞介素1β能促进冠状动脉平滑肌细胞中斑块稳定相关标记物基质金属蛋白酶3的表达,可能是炎症在急性冠状动脉综合征发生发展中的作用机制之一。

关键词：冠状动脉疾病白细胞介素1 基质金属蛋白酶类

Current status of drug therapy for chronic hepatitis B

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World Journal of Gastroenterology 2025年第2期31卷 1-12页

作者： Chuang jiang Zhi-Hong Zhang Jia-Xin Li Division of Liver Surgery Department of General Surgery and Laboratory of Liver Surgeryand State Key Laboratory of Biotherapy and Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of General Surgery Dafang County People's HospitalBijie 551600Guizhou ProvinceChina

In this editorial,we comment on the article by Meng et *** hepatitis B(CHB)is a significant global health problem,particularly in developing *** B virus(HBV)infection is one of the most important risk factors for cirrhosis and hepatocellular *** and treatment of HBV are key measures to reduce *** present,drug therapy can effectively control virus replication and slow disease progression,but completely eliminating the virus remains a ***-HBV treatment is a long-term process,and there are many kinds of antiviral drugs with different mechanisms of action,it is essential to evaluate the safety and efficacy of these drugs to reduce side effects and improve patients’*** will summarize the current status of CHB drug treatment,hoping to provide a reference for the selection of clinical antiviral drugs.

关键词： Chronic hepatitis B Hepatitis B virus Anti virus Drug treatment Therapy

Specific Heat and Interface Energy of Nanostructured ZrAlNiCuCo Alloy

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Journal of Materials Science & Technology 1997年第4期13卷 284-288页

作者： jiang, Q Sui, zx Li, JC Xu, XY Dept. of Materials Science and Engineering Jilin University of Technology Changchun 130025 China

The specific heat of the nanostructured crystals, the glass, the liquid and the crystals for a ZrAlNiCuCo alloy has been measured by using the differential scanning calorimeter. The specific heat value and its temperature coefficient of the nanostructured crystals are larger than that of the relaxed glass and of the crystals. Through measuring crystallization enthalpies and specific heat of the alloy in different states, the enthalpy differences among the crystals, the liquid and the nanostructured crystals are calculated and the interface energies of nanostructured crystals are estimated. The stability and the grain size limit of the nanostructured crystals are discussed.

关键词： Nanostructured materials

Molecular modeling of rice Rubisco

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Chinese Journal of Chemistry 1997年第4期15卷 353-360页

作者： Dai, WW jiang, GF He, YN Xia, zx Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

Molecular model of rice Rubisco has been constructed by molecular modelling method on a computer graphics workstation using graphics software TOM-FRODO and QUANTA/CHARMm, based on the three-dimensional structure of spinach Rubisco. The molecular modelling was carried out in the following procedure: amino-acid sequence substitution; manual adjustment of the initial model; and energy minimization. The three-dimensional structure of the quaternary complex Rubisco CO2 . Mg2+. CABP of rice Rubisco has also been modelled and optimized using the program package XPLOR. The main-chain structure of rice Rubisco does not show obvious difference from that of spinach Rubisco, and the side-chains are located in the most favorable positions and orientations. The active-site structures are basically the same for the two species. The mutant Leu335-->Arg of rice Rubisco has been designed using the graphics software TURBO-FRODO and the program package XPLOR. Energy computation suggests that this mutation might increase the carboxylation activity.

关键词： rice Rubisco molecular modeling energy minimization mutant design