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Glycosphingolipids are critical for Dengue virus replication, but not virus binding and entry

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Glycosphingolipids are critical for Dengue virus replication...

Functional characterization of two defensins H1DFS1 & HlDFS2 from a hard tick,Haemaphysalis longicornis

第十届全国免疫学学术大会

作者： jianfeng dai 苏州大学生物医学研究院

Dengue virus(DENV),a member of the flavivirus genus of the Flaviviridae family,causes the most prevalent arthropodborne viral disease of humans ***,there is no approved vaccine,nor any antiviral drugs for the treatment of ***(GSLs),amphipathic molecules consisting of a ceramide and sugar residues,are ubiquitous cellular components of eukaryotic membrane ***,GSLs were reported to involve in virus infection by regulating various steps of viral-host cell ***,the distinct role of GSLs during DENV infection remains *** this study,we used mouse melanoma B16 cells and their GSL-deficient mutant counterpart GM95 cells to study the influence of GSLs on DENV *** have found that GSL-deficient GM95 cells were highly resistant to DENV infection when compared with B16 wild type *** of B16 cells with GSLs synthesis inhibitor or impairing the critical enzyme MT3GAL5 required for the synthesis of GSLs GM3 by RNAi both significantly inhibited dengue virus ***,the decreased DENV infection was partially rescued by addition of exogenous GSLs GM3 into GM95 *** binding and entry,as determined by q PCR of viral genes or TRITClabeled virus imaging,were not impaired in GM95 cells when compared with B16 wt *** further confirmed thatGLSs are critical for DENV RNA replication by directly transfecting a DNA-based dengue virus replicon into B16 and GM95 *** replicon replication efficiency significantly increased in B16 cells when compared to GM95 ***,GSLs GM3 were co-localized with DENV double strand RNA and viral replication complex inside the cytoplasm of B16 *** together,these data indicated that GSLs are not required for DENV virus attachment and endocytosis,however,participate in virus *** GSLs could be a novel strategy to inhibit DENV infection.

关键词： Dengue virus Glycosphingolipids viral replication

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Functional characterization of two defensins H1DFS1 & HlDFS2...

Detection of low-concentration EGFR with a highly sensitive optofluidic resonator

第十二届全国免疫学学术大会

作者： dai jianfeng Soochow University

Background:Ticks are second to mosquitoes as vectors of human arthropod borne *** rely heavily on antimicrobial peptides(AMPs) to defend against microbes and defensins are major components of innate immunity in ***:Two novel defensin genes,named H1DFS1 and H1DFS2,were identified from a cDNA library of the hard tick Haemaphysalis longicornis collected from southeast *** peptides encoded by both genes shares typical features of type-2 arthropod defensin *** expressions of both genes increased in ticks during blood *** synthetic minimum functional peptides H1DFS1 and H1DFS2 showed broad spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria,such as Staphylococcus aureus,Micrococcus luteus,Mycobacterium bovis,Escherichia coli and Borrelia ***,H1DFS1 and H1DFS2 exhibit bactericidal activity to some drug resistant bacteria(Staphylococcus epidermidis and Acinetobacter baumannii).H1DFS1,but not H1DFS2,showed inhibitory activity against fungus Candida albicans.H1DFS1 and H1DFS2 had no significant hemolysis effect on human erythrocytes at low concentrations and did not impair mammalian cell ***,H1DFS1 and H1DFS2 significantly protected mice against lethal infection by Staphylococcus aureus and Micrococcus ***:H1DFS1 and H1DFS2 are two novel functional defensins from the hard tick Haemaphysalis *** showed bactericidal activity against various Gram-positive and Gram-negative bacteria and significantly protect mice against lethal bacterial ***,H1DFS1 and H1DFS2 can be introduced to the medical field as new drug candidates with antibacterial activity.

关键词： Defensin antimicrobial peptide(AMP) antibiotic resistant bacteria tick Haemaphysalis longicorni

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Chinese Optics Letters 2017年第9期15卷 90-93页

作者：尚剑锋戴海浪邹芸陈险峰 State Key Laboratory of Advanced Optical Communication Systems and Networks Department of Physics and AstronomyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Key Laboratory of Crime Science Evidence Shanghai Research Institute of Criminal Science and TechnologyShanghai 200083China

A hollow-core metal-cladding waveguide（HCMW） optofluidic resonator that works based on a free-space coupling technique is designed. An HCMW can excite ultra-high-order modes（UOMs） at the coupled angle, which can be used as an optofluidic resonator to detect alterations of the epidermal growth factor receptor（EGFR）concentration. Theoretical analysis shows that the UOMs excited in the HCMW have a highly sensitive response to the refractive index（RI） variation of the guiding layer. An EGFR solution with a 0.2 ng/mL alteration is detected, and the RI variation caused by the concentration alteration is about 2.5 × 10^（-3）.

关键词： Detection of low-concentration EGFR with a highly sensitive optofluidic resonator

Defensins as a promising class of tick antimicrobial peptides: a scoping review

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Infectious Diseases of Poverty 2022年第3期11卷 84-84页

作者： Jiahui Wu Xia Zhou Qiaoqiao Chen Zhiqiang Chen Jinyu Zhang Lele Yang Yuxuan Sun Guohui Wang jianfeng dai Tingting Feng Institute of Biology and Medical Sciences Jiangsu Key Laboratory of Infection and ImmunitySoochow UniversitySuzhouChina 不详 School of Life Science and Technology Weifang Medical UniversityWeifangChina

Background: Ticks are hematophagous parasites that transmit an extensive range of pathogens to their vertebrate *** can destroy invading microorganisms or alleviate infection via their rudimentary but orchestrated innate immune *** peptides(AMPs)are important components of tick innate *** these humoral effector molecules,defensins are well-studied and widely identified in various species of Ixodidae(hard ticks)and Argasidae(soft ticks).This review was aimed at presenting the characterization of tick defensins from structure-based taxonomic status to antimicrobial *** text: All published papers written in English from 2001 to May 2022 were searched through PubMed and Web of Science databases with the combination of relevant terms on tick *** on identification and characterization of tick defensins were *** the 329 entries retrieved,57 articles were finally eligible for our scoping *** defensins mainly belong to the antibacterial ancient invertebrate-type defensins of the cis-defensins *** are generally small,cationic,and amphipathic,with six cysteine residues forming three intra-molecular disulfide *** defensins primarily target membranes of a variety of pathogens,including Gram-positive and Gram-negative bacteria,fungi,viruses,and *** tick defensins have a high degree of variability,we summarize their common biological properties and enumerate representative *** with the various and potent antimicrobial activities,the role of tick defensins in determining vector competence is ***: Due to their broad-spectrum antimicrobial activities,tick defensins are considered novel candidates or targets for controlling infectious diseases.

关键词： Tick Antimicrobial peptide Defensin Antimicrobial activity

Study on Transmission Planning of Cascade Hydropower Stations along Lancang River Basin

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Electricity 2012年第1期23卷 26-31页

作者： Cui Wenjia Du Zhongming dai jianfeng China Power Engineering Consulting Group Corporation

Most of the hydropower projects in Southwest China and the adjacent foreign regions will be put into operation between 2015 and 2020, which will bring some difficulties for reasonable accommodation and delivery of electric energy. In this paper the author studies the development scale, development schedule, accommodation and transmission schemes of the cascade hydropower stations along the Lancang River basin, one of the five large basins in China, based on the load characteristics of grids at both the sending end and the receiving end, the strategy of complementary utilization of thermal power and hydropower, the advanced transmission technologies, and the optimal economic performance. The study results show that, the cascade hydropower stations on the upper reaches of the Lancang River in Yunnan should mainly serve Guangdong Province, with proper planning of partly serving Yunnan Province during dry seasons. The transmission schemes should adopt UHVDC, UHVAC, and single-tower double-circuited HVDC transmission scheme according to the transmission capacity and distance.

关键词：梯级水电站澜沧江流域输电规划特高压直流输电中国西南地区传输方案澜沧江上游水电项目

Preparation and particle size characterization of Cu nanoparticles prepared by anodic arc plasma

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Rare Metals 2006年第2期25卷 172-176页

作者： WEI Zhiqiang XIA Tiandong FENG Wangjun dai jianfeng WANG Qing LI Weixue YAN Pengxun School of Sciences Lanzhou University of Technology Lanzhou 730050 China College of Materials Science and Engineering Lanzhou University of Technology Lanzhou 730050 China Institute of Plasma and Metal Materials Lanzhou University Lanzhou 730000 China

Copper nanoparticles were successfully prepared in large scale by means of anodic arc discharging plasma method in inert atmosphere. The particle size, specific surface area, crystal structure, and morphology of the samples were characterized by X-ray diffraction （XRD）, BET equation, transmission electron microscopy （TEM）, and the corresponding selected area electron diffraction （SAED）. The experimental results indicate that the crystal structure of the samples is fcc structure the same as that of the bulk materials. The specific surface area is 11 m^2/g, the particle size distribution is 30 to 90 nm, and the average particle size is about 67 nm obtained from TEM and confirmed from XRD and BET results. The nanoparticles with uniform size, high purity, narrow size distribution and spherical shape can be prepared by this convenient and effective method.

关键词： metal materials Cu nanoparticles anodic arc plasma particle size structure

基于电力CPS联合仿真的主配一体化电网负荷转供方法研究

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太阳能学报 2023年第11期44卷 530-537页

作者：杨志淳沈煜杨帆张顺道戴剑丰国网湖北省电力有限公司电力科学研究院武汉430077 东南大学电气工程学院南京210096 南京邮电大学自动化学院、人工智能学院南京210032

分布式光伏和风电等分布式电源的大量并网、电力信息物理系统(CPS)的快速发展等对电力系统的运行调度带来不可估算的影响,针对紧急状态下提高电力系统供电可靠性的问题,该文提出面向主配一体化电网的负荷转供方法。首先,考虑光伏和风电... 详细信息

分布式光伏和风电等分布式电源的大量并网、电力信息物理系统(CPS)的快速发展等对电力系统的运行调度带来不可估算的影响,针对紧急状态下提高电力系统供电可靠性的问题,该文提出面向主配一体化电网的负荷转供方法。首先,考虑光伏和风电自身特点建立分布式发电模型;其次,根据负荷重要性对负荷进行分类,并计及通讯延时计算负荷恢复率;然后,以切负荷与网损为目标函数,并考虑网络约束,通过构建遗传算法对目标函数寻优求解;最后,在电力CPS联合仿真平台上进行验证,结果表明所提方法能更接近实际运行工况,具有更好的负荷恢复效果,从而减小社会经济损失。

关键词：光伏发电风电分布式发电信息物理系统遗传算法负荷转供

Identification of an Immunosuppressive Tick Protein, tCystatin, from Dermacentor silvarum collected from Northeast China

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Identification of an Immunosuppressive Tick Protein, tCystat...

第十一届全国免疫学学术大会

作者： Sun Ta dai jianfeng Institute of Biology and Medical Sciences Soochow University

Background: Ticks are blood-feeding arthropods and transmit numerous human pathogens. Ticks secrete immunosuppressant molecules which can inhibit host inflammatory and immune responses and provide survival advantages to pathogens at tick bleeding sites in hosts. Mammalian Cathepsins play important roles in innate and adaptive immune responses, include in the physiological process of apoptosis;antigen processing and presentation. Cystatins are widely expressed in animals and other species which are native inhibitors of cathepsins. Methods and Results: In this study, we characterized a novel tick secreted protein, t Cystatin, from Dermacentor silvarum collected from Northeast China. Recombinant t Cystatin inhibited enzymatic activity of human Cathepsin L, B and S in a dose dependent manner. t Cystatin suppressed inflammatory cytokine production from mouse bone marrow derived macrophage which stimulated with LPS or Borrelia burgdorferi. t Cystatin inhibited the activation and the surface expression of CD80 and CD86 of bone marrow derived macrophage and dendritic cells. Meanwhile, t Cystatin also impaired antigen degradation in DCs. In mouse inflammatory diseases models, t Cystatin can relief the joint inflammation induced by Complete Freund’s adjuvant. t Cystatin also suppressed Borrelia burgdorferi induced joint swelling and antibody production. Conclusions: These data suggested that t Cystatin is a novel immunosuppressive protein and can be potentially used in treatment of inflammatory diseases.

关键词： cathepsins tick cystatin immunosuppressant

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数控机械加工效率优化措施探讨

五金科技 2023年第6期51卷 62-65页

作者：戴建峰衢州市工程技术学校(衢州市技师学院) 浙江衢州324000

制造业是国民经济的基础产业,在国民经济发展以及维护国家经济安全中发挥着举足轻重的作用。当前我国已经成为首屈一指的制造大国,制造业产值居全球首位,是美国、日本、德国制造业产值的总和。推动制造业由大到强是当前我国制造业发展... 详细信息

制造业是国民经济的基础产业,在国民经济发展以及维护国家经济安全中发挥着举足轻重的作用。当前我国已经成为首屈一指的制造大国,制造业产值居全球首位,是美国、日本、德国制造业产值的总和。推动制造业由大到强是当前我国制造业发展的核心任务,而数控机械加工效率优化则在增强制造业竞争力中发挥着重要的作用。受设备因素、工艺因素、时间因素、管理因素、信息因素、人员因素等多重因素的影响,当前数控机械加工面临着效率不高的问题。文章基于此,以数控机械加工效率优化措施为研究内容,从严格数控机械的选择、改进数控机械的工艺、控制单件加工的时间、优化数控机械的管理、深化信息技术的应用、加强专业人才的培养五个方面提出策略。

关键词：数控机械加工效率优化措施